ISSN:
1573-4919
Schlagwort(e):
DNA binding proteins
;
DNase-I hypersensitive sites
;
chromatin transcription
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
,
Medizin
Notizen:
Summary Brief digestion of sarcoma-180 chromatin (≤ 5%) by pancreatic DNase-I releases six non-histone proteins with Mol. wt. 21.5K, 26K, 72.5K, 77K, 90K and 120K dalton and pI values ranging from 4.7 to 12.4. The protein of Mol. wt. 77K dalton was obtained at high alkaline range of pH = 12.4. The antibodies against these proteins induce dose dependent inhibition in transcription of native chromatin. Results suggest a role of these proteins in positive control of gene transcription in sarcoma-180 cells.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00229684
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