Publication Date:
2018-11-03
Description:
A mosaic and mottle symptom was observed on several cucumber cultivations in Java, Indonesia. The study was aimed to characterize the causal virus of typical Tobamovirus- like symptoms. Symptomatic leaves were collected randomly from each location in Nganjuk, Kediri, Tulungagung (East Java), Brebes and Klaten (Central Java), Kulon Progo (Yogyakarta), and Subang, Indramayu, Bogor (West Java). The diseases frequency was determined by dot blot immuno binding assays (DIBA) test using specific antiserum of Cucumber green mottle mosaic virus (CGMMV). Furthermore, the Tobamovirus was detected by RT-PCR, then cloned into TA-cloning vector, and the DNA was sequenced. The disease frequency of CGMMV in East Java, Central Java, Yogyakarta, and West Java was up to 24.44%, 9.09%, 0%, and 5.49%, respectively. CGMMV was unable to be amplified by specific primer, however it was able to be amplified by universal primer of Tobamovirus with sized ± 800 bp. Nucleotide sequ...
Print ISSN:
1755-1307
Electronic ISSN:
1755-1315
Topics:
Geography
,
Geosciences
,
Physics
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