Publication Date:
1999-01-05
Description:
Whether a single major histocompatibility complex (MHC)-bound peptide can drive the positive selection of large numbers of T cells has been a controversial issue. A diverse population of self peptides was shown to be essential for the in vivo development of CD4 T cells. Mice in which all but 5 percent of MHC class II molecules were bound by a single peptide had wild-type numbers of CD4 T cells. However, when the diversity within this 5 percent was lost, CD4 T cell development was impaired. Blocking the major peptide-MHC complex in thymus organ culture had no effect on T cell development, indicating that positive selection occurred on the diverse peptides present at low levels. This requirement for peptide diversity indicates that the interaction between self peptides and T cell receptors during positive selection is highly specific.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Barton, G M -- Rudensky, A Y -- New York, N.Y. -- Science. 1999 Jan 1;283(5398):67-70.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Molecular and Cellular Biology Program of the University of Washington and Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9872742" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigen Presentation
;
CD4-Positive T-Lymphocytes/cytology/*immunology/metabolism
;
CD8-Positive T-Lymphocytes/cytology/immunology/metabolism
;
Cells, Cultured
;
Histocompatibility Antigens Class II/*immunology/metabolism
;
Lymphocyte Activation
;
Lymphocyte Culture Test, Mixed
;
Mice
;
Mice, Knockout
;
Mice, Transgenic
;
Peptides/*immunology/metabolism
;
Receptors, Antigen, T-Cell/*immunology
;
Recombinant Fusion Proteins/metabolism
;
Spleen/immunology
;
Thymus Gland/immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
