Publication Date:
1995-12-22
Description:
Reliable germline transformation is required for molecular studies and ultimately for genetic control of economically important insects, such as the Mediterranean fruit fly (medfly) Ceratitis capitata. A prerequisite for the establishment and maintenance of transformant lines is selectable or phenotypically dominant markers. To this end, a complementary DNA clone derived from the medfly white gene was isolated, which showed substantial similarity to white genes in Drosophila melanogaster and other Diptera. It is correlated with a spontaneous mutation causing white eyes in the medfly and can be used to restore partial eye color in transgenic Drosophila carrying a null mutation in the endogenous white gene.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zwiebel, L J -- Saccone, G -- Zacharopoulou, A -- Besansky, N J -- Favia, G -- Collins, F H -- Louis, C -- Kafatos, F C -- New York, N.Y. -- Science. 1995 Dec 22;270(5244):2005-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8533095" target="_blank"〉PubMed〈/a〉
Keywords:
*ATP-Binding Cassette Transporters
;
Amino Acid Sequence
;
Animals
;
Animals, Genetically Modified
;
Base Sequence
;
Cloning, Molecular
;
Diptera/chemistry/*genetics
;
*Drosophila Proteins
;
Drosophila melanogaster/genetics
;
Eye Color/genetics
;
Eye Proteins/chemistry/*genetics
;
*Genes, Insect
;
Genetic Markers
;
Insect Hormones/chemistry/genetics
;
Molecular Sequence Data
;
Mutation
;
Phenotype
;
Sequence Alignment
;
*Transformation, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
