Publication Date:
1992-05-01
Description:
A DNA-nicking activity was detected in the sera of patients with various autoimmune pathologies and was shown to be a property of autoantibodies. The DNA hydrolyzing activity, which was purified by affinity and high-performance liquid chromatography, corresponded in size to immunoglobulin M (IgM) and IgG and had a positive response to antibodies to human IgG. The DNA hydrolyzing autoantibodies were stable to acid shock and yielded a DNA degradation pattern that was different from that of deoxyribonuclease (DNase) I and blood DNase.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shuster, A M -- Gololobov, G V -- Kvashuk, O A -- Bogomolova, A E -- Smirnov, I V -- Gabibov, A G -- New York, N.Y. -- Science. 1992 May 1;256(5057):665-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉V.A. Engelhardt Institute of Molecular Biology, Academy of Sciences of Russia, Moscow.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1585181" target="_blank"〉PubMed〈/a〉
Keywords:
Acetates/pharmacology
;
Acetic Acid
;
Autoantibodies/isolation & purification/*metabolism
;
Autoimmune Diseases/*metabolism
;
Chromatography, High Pressure Liquid
;
DNA/*metabolism
;
DNA Polymerase I/metabolism
;
Deoxyribonuclease I/metabolism
;
Electrophoresis, Agar Gel
;
Humans
;
Immunoglobulin G/isolation & purification/metabolism
;
Immunoglobulin M/isolation & purification/metabolism
;
Kinetics
;
Lupus Erythematosus, Systemic/immunology
;
Plasmids
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
