ALBERT

Description:    High-performance counter-current chromatography has been used for the separation of delphinidin-3- O -sambubioside, cyanidin-3- O -sambubioside and p -coumaric acid from crude extract of cranberry. The separation was performed with a two-phase solvent system composed of butanol/0.05% aqueous trifluoroacetic acid/methanol at a volume ratio of 4:5:1. The two-phase solvent system was selected following the determination of partition coefficients ( K ) in a range of solvent systems using a robotic solvent system selection method. Analytical scale CCC confirmed that this phase system separated the components from a crude cranberry extract (40 mg scale) with acceptable purities. Preparative CCC of 400 mg of crude yielded 4.2 mg of p -coumaric acid at a purity of over 98%, 3.6 mg of delphinidin-3- O -sambubioside at a purity of over 97% and 4.5 mg of cyanidin-3- O -sambubioside at a purity of 73%, which was further purified by preparative high-performance liquid chromatography to yield 3 mg cyanidin-3- O -sambubioside at 95% purity. The identification of delphinidin-3- O -sambubioside, cyanidin-3- O -sambubioside and p -coumaric acid was performed by ESI-MS, 1H-NMR and 13C-NMR spectra. Content Type Journal Article Pages 1-7 DOI 10.1007/s10337-011-2076-5 Authors Yun Wei, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, Beijing, 100029 China M. Razwan Sardar, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, Beijing, 100029 China Ian A. Sutherland, Advanced Bioprocessing Centre, Brunel Institute for Bioengineering, Brunel University, Uxbridge, Middlesex, UB8 3PH UK Derek Fisher, Advanced Bioprocessing Centre, Brunel Institute for Bioengineering, Brunel University, Uxbridge, Middlesex, UB8 3PH UK Journal Chromatographia Online ISSN 1612-1112 Print ISSN 0009-5893