Publication Date:
2018
Description:
〈sec〉〈st〉Synopsis〈/st〉〈p〉〈textbox textbox-type="graphic"〉〈p〉〈inline-fig〉〈/inline-fig〉〈/p〉〈/textbox〉〈/p〉
〈p〉Maternal transcripts are degraded during oocyte maturation. A new mouse model demonstrates that 〈i〉Cnot6l〈/i〉, one of four genes encoding catalytic subunits of the mRNA deadenylation complex CCR4–NOT, is preferentially expressed in oocytes and mediates meiosis-coupled maternal mRNA decay.〈/p〉
〈p〉 〈l type="unord"〉〈li〉〈p〉Genetic deletion of 〈i〉Cnot6l〈/i〉 impaired deadenylation and degradation of a subset of maternal mRNAs during mouse oocyte maturation.〈/p〉〈/li〉
〈li〉〈p〉〈i〉Cnot6l〈/i〉-deficient female mice were severely subfertile.〈/p〉〈/li〉
〈li〉〈p〉Aberrant translation of undegraded mRNAs in the 〈i〉Cnot6l〈/i〉 knockout caused microtubule–chromosome organization defects, activation of spindle assembly checkpoint and meiotic cell cycle arrest at prometaphase.〈/p〉〈/li〉
〈li〉〈p〉Recruitment of distinct RNA-binding adaptor proteins by different CCR4–NOT subunits ensures stage-specific degradation of maternal mRNAs.〈/p〉〈/li〉
〈li〉〈p〉CNOT6L and other CCR4–NOT components are important downstream effectors of ERK1 and ERK2 in regulating spindle assembly and meiotic cell cycle progression in oocytes.〈/p〉〈/li〉〈/l〉 〈/p〉〈/sec〉
Print ISSN:
0261-4189
Electronic ISSN:
1460-2075
Topics:
Biology
,
Medicine
