ISSN:
1745-4565
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The pork tonsils are an important carrier of Salmonella, which could be involved in the contamination of pork products during the slaughter process. This paper reports a 23S rRNA fluorescent in situ hybridization (FISH) method used as a rapid screening tool for Salmonella detection in tonsils of slaughtered pigs and its comparison with the conventional culture method. As a rapid screening method, the FISH technique would reduce the high volume of negative samples that are routinely analyzed, indicating presumptive positive samples in a real and practical time. The use of a Sal3 probe allowed the rapid (7 h) Salmonella detection in 16 (34%) of 47 naturally contaminated tonsils, without pre-enrichment. Salmonella was isolated by the culture method in six samples that were also FISH-positive samples, and FISH failed to identify only one of the culture-positive samples. The results indicate the potential of this technique as a rapid screening method for detecting Salmonella in tonsils from pork slaughtered for consumption.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1745-4565.2005.00563.x