ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The N-terminal portion (amino acids 1303–1367) of the type 1 ryanodine receptor D2 region is thought to be critical for excitation–contraction coupling in skeletal muscle. A segment of the D2 region (amino acids 1317–1355) was expressed as a glutathione S-transferase fusion protein (GST-D2) and then crystallized at room temperature using ammonium sulfate as precipitant. Using a newly developed cryo-soaking method, complete native data sets were measured to a resolution of 2.2 Å using synchrotron radiation. The crystal was found to be hexagonal, belonging to space group P6322, with unit-cell parameters a = b = 116.1, c = 77.9 Å.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444999008574