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Application of a thermalin situ reaction for fluorimetric detection of carbohydrates on NH2-layers (1990)

Klaus, R. ; Fischer, W. ; Hauck, H. E.

Springer

Chromatographia 29 (1990), S. 467-472

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ISSN: 1612-1112

Keywords: Thin-layer chromatography ; NH2-layers ; Carbohydrates ; Fluorescence detection

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary Based on experience in the purely thermally induced (no analytical reagents) fluorimetric detection of glucose and fructose after thin-layer chromatographic separation on NH2-layers, the application of this method to other sugars is described. After separation with mobile phases containing pyridine, this “dry”in situ derivatization permits a very simple qualitative and quantitative method of detection for the 20 carbohydrates studied.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02261396

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