ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Amino acids
;
O-phthaldialdehyde-2-mercaptoethanol
;
Apples
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A rapid and sensitive method for the simultaneous determination of primary amino acids in apple is described. After sample preparation, amino acids were derivatized with o-phthaldialdehyde/2-mercaptoethanol and separated on a reversed phase column with a gradient of phosphate buffer-tetrahydrofuran-methanol as the mobile phase. Detection was carried out with a fluorescence detector at excitation and emission wavelengths of 340 nm and 425 nm respectively. Recovery studies showed good results for all substances (91–109%) (with coefficients of variation ranging, from 0.1 to 9.0%). This method was applied to the monitoring of amino acids during the ripening of apples.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02268703
