ISSN:
1432-0991
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Diffusion coefficients and mean diameters of estuarine bacterial isolates and particulates from Bayboro Harbor water, St. Petersburg, FL, were measured by photon correlation spectroscopy (PCS) employing a Coulter N4 Submicron Particle Analyzer. Mean diameters of formalin-killed bacteria by PCS agreed with those obtained from Nomarski Interference and epifluorescence microscopy. Diffusion coefficients of motile bacteria were 1.3–4.4 times greater than for formalin-killed bacteria, and PCS-estimated diameters were accordingly smaller. Analysis of Bayboro Harbor water by PCS indicated a highly variable distribution of particle diameters in unfiltered water samples. Filtration through 3-, 1-, 0.4-, and 0.2-μm Nucleopore filters resulted in progressively smaller, reproducible particle diameter distributions, which again agreed with measurements made by epifluorescence microscopy.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01576040
