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Production of somatic hybrids of moss by electrofusion (1985)

Watts, J. W. ; Doonan, J. H. ; Cove, D. J. ; [et al.]

Springer

Molecular genetics and genomics 199 (1985), S. 349-351

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ISSN: 1617-4623

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Mixtures of protoplasts of two auxotrophic mutants of Physcomitrella patens, one requiring thiamine (aneurine), the other p-aminobenzoic acid, have been subjected to electrofusion. The protoplasts were aligned in an alternating electric field (500 KHz, 20 V RMS/cm) and induced to fuse by a brief DC pulse (800 V/cm, time constant lms). After culture, first on complete medium and then on selective medium, hybrid plants were obtained at a frequency of 3%. One hybrid with a morphology typical of polyploidy was also observed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00330279

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