ISSN:
1432-203X
Keywords:
Pollen
;
Polygalacturonase
;
Promoter
;
Brassica
;
Transformation
;
GUS
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A 647-bp 5′-flanking fragment obtained from genomic clone Sta 44G(2) belonging to a family of polygalacturonase genes expressed inBrassica napus pollen was fused to theβ-glucuronidase (GUS) marker gene. This fusion construct was introduced intoB. napus plants viaAgrobacterium tumefaciens transformation. Analysis of the transgenicB. napus plants revealed that this promoter fragment is sufficient to direct GUS expression specifically in the anther and that GUS activity increases in pollen during maturation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01146776