ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary 4-O-\-d-Glucopyranosyl-d-xylose (GX) was synthesized from equimolar amounts of d-xylose and α-d-glucose-1-phosphate (G-1-P) using acetone-treated cells of Cellvibrio gilvus. It was found that ethanol treatment of acetone-treated cells selectively removed phosphoglucomutase activity, which competes with cellobiose phosphorylase for G-1-P in the synthetic reaction. The yield of synthesis was 60%, based on d-xylose used. GX was purified by charcoal column chromatography with a 32% yield based on d-xylose. Nuclear magnetic resonance and fast atm bombardment mass data of GX are presented. The possibility for this saccharide to be used as a new foodstuff is also discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00166776
