ISSN:
1573-5001
Keywords:
RN4 structure determination
;
coupling constants
;
13C block labelled RNA
;
chemical synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract A new experiment for the measurement of nJ(C,P) coupling constants along the phosphodiester backbone in RNA and DNA based on a quantitative-J HCP experiment is presented. In addition to coupling constants, in which a carbon atom couples to only one phosphorus atom, both the intraresidual 3J(C4′i,Pi) and the sequential 3J(C4′i,Pi+1) for the C4′ resonances that couple to two phosphorus atoms can be obtained. Coupling constants obtained by this new method are compared to values obtained from the P-FIDS experiment. Together with 3J(H,P) coupling constants measured using the P-FIDS experiment, the backbone angles β and ∈ can be determined.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008258226683