ISSN:
0006-3592
Keywords:
Saccharomyces cerevisiae
;
δ sequences cloned genes
;
integration
;
stability
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The chromosomal δ sequences of the yeast Saccharomyces cerevisiae were employed as recombination sites to integrate the bacterial neor gene and the yeast SUC2 gene into the yeast genome. A dominate selection method employing the aminoglycoside antibiotic G418 was used. Transformation efficiencies and growth behaviors of the transformants were studied. Transformants were obtained with more than 40 integrations; the majority of insertions were tandem with a maximum of three different insertion sites utilized at one time. After 70-100 generations of growth in nonselective medium, the high copy number SUC2-neor integrants were found to be unstable; only minor instability was observed for the neor and low copy number SUC2-neor integrants. © 1996 John Wiley & Sons, Inc.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
