ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Bovine salivary proteins have been shown to be correlated with bloat susceptibility in cattle. Due to the presence of the high molecular weight mucoprotein in whole saliva these proteins have been difficult to purify. This difficulty has now been overcome by using electrophoretic procedures. The purification protocol consisted of electrophoretic concentration of the saliva, separation of the proteins by polyacrylamide gel electrophoresis, excision of the protein bands followed by electroelution and concentration. Purified proteins were obtained from bands 4, 5, 6, 7, 8 and 9/10. The molecular weights of the intact proteins were between 40 300 and 112 400 on gel chromatography and the subunit molecular weights were between 15000 and 65 400. The isoelectric points were within the pH range of 3.6 to 5.1. Antisera were produced against protein bands 7 and 8 and these were used to determine antigenic relationships between proteins and as a means of quantifying the proteins by the Laurell rocket immunoassay.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150080705
