ISSN:
1076-5174
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
The analysis of oligonucleotides by mass spectrometry should give information on both the molecular mass and sequence. Fast atom bombardment mass spectrometry gives both in one step. Until now glycerol, thioglycerol and triethanolamine have been used as matrices for the analysis of oligonucleotides by fast atom bombardment mass spectrometry (FABMS), but there have been no comparative studies for matrix selection for these specific analytes. In this work, the influence of diethanolamine, glycerol, 1-thioglycerol and triethanolamine on the analysis of unprotected trimer up to heptamer oligoribonucleotides was studied using negative-ion FABMS on a sector-type mass spectrometer. Collision-induced dissociation MS/MS measurements were performed and the fragmentation patterns in MS and MS/MS were compared.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jms.1190300314
