Publication Date:
1990-06-29
Description:
In Rous sarcoma virus (RSV)-infected chickens, wounding leads to tumor formation with nearly 100% frequency in tissues that would otherwise remain tumor-free. Identifying molecular mediators of this phenomenon should yield important clues to the mechanisms involved in RSV tumorigenesis. Immunohistochemical staining showed that TGF-beta is present locally shortly after wounding, but not unwounded controls. In addition, subcutaneous administration of recombinant transforming growth factor-beta 1 (TGF-beta 1) could substitute completely for wounding in tumor induction. A treatment protocol of four doses of 800 nanograms of TGF-beta resulted in v-src-expressing tumors with 100% frequency; four doses of only 10 nanograms still led to tumor formation in 80% of the animals. This effect was specific, as other growth factors with suggested roles in wound healing did not elicit the same response. Epidermal growth factor (EGF) or TGF-alpha had no effect, and platelet-derived growth factor (PDGF) or insulin-like growth factor-1 (IGF-1) yielded only occasional tumors after longer latency. TGF-beta release during the wound-healing response may thus be a critical event that creates a conducive environment for RSV tumorigenesis and may act as a cofactor for transformation in this system.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sieweke, M H -- Thompson, N L -- Sporn, M B -- Bissell, M J -- New York, N.Y. -- Science. 1990 Jun 29;248(4963):1656-60.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cell and Molecular Biology Division, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2163544" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies
;
Chickens
;
Epidermal Growth Factor/pharmacology
;
Humans
;
Immunoenzyme Techniques
;
Insulin-Like Growth Factor I/pharmacology
;
Platelet-Derived Growth Factor/pharmacology
;
Recombinant Proteins/pharmacology
;
Sarcoma, Avian/complications/*pathology
;
Swine
;
Transforming Growth Factors/analysis/*pharmacology
;
Wound Healing
;
Wounds and Injuries/complications/*pathology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
