Publication Date:
2011-06-12
Description:
In the blowfly Phormia regina , experience of simultaneous feeding with d -limonene exposure inhibits proboscis extension reflex (PER) due to decreased tyramine (TA) titer in the brain. To elucidate the molecular mechanism of TA signaling pathway related to the associated feeding behavior, we cloned cDNA encoding the octopamine/TA receptor (PregOAR/TAR). The deduced protein is composed of 607 amino acid residues and has 7 predicted transmembrane domains. Based on homology and phylogenetic analyses, this protein belongs to the OAR/TAR family. The PregOAR/TAR was mainly expressed in head, with low levels of expression in other tissues at adult stages. Gene expression profile is in agreement with a plethora of functions ascribed to TA in various insect tissues. The immunolabeled cell bodies and processes were localized in the medial protocerebrum, outer layer of lobula, antennal lobe, and subesophageal ganglion. These results suggest that decrease of TA level in the brain likely affects neurons expressing PregOAR/TAR , causing mediation of the sensitivity in the sensillum and/or output of motor neurons for PER. Content Type Journal Article Pages 1-4 DOI 10.1007/s00114-011-0806-z Authors Yuko Ishida, Department of Biology, Graduate School of Science, Kobe University, 1–1 Rokkodai-cho, Nada-ku, Kobe, 657–8501 Japan Mamiko Ozaki, Department of Biology, Graduate School of Science, Kobe University, 1–1 Rokkodai-cho, Nada-ku, Kobe, 657–8501 Japan Journal Naturwissenschaften Online ISSN 1432-1904 Print ISSN 0028-1042
Print ISSN:
0028-1042
Electronic ISSN:
1432-1904
Topics:
Biology
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Chemistry and Pharmacology
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Natural Sciences in General
