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Nanospectrofluorometry inside single living cell by scanning near-field optical microscopy (2001)

Lei, F. H. ; Shang, G. Y. ; Troyon, M.

Woodbury, NY : American Institute of Physics (AIP)

Applied Physics Letters 79 (2001), S. 2489-2491

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ISSN: 1077-3118

Source: AIP Digital Archive

Topics: Physics

Notes: Near-field fluorescence spectra with subdiffraction limit spatial resolution have been taken in the proximity of mitochondrial membrane inside breast adenocarcinoma cells (MCF7) treated with the fluorescent dye (JC-1) by using a scanning near-field optical microscope coupled with a confocal laser microspectrofluorometer. The probe–sample distance control is based on a piezoelectric bimorph shear force sensor having a static spring constant k=5 μN/nm and a quality factor Q=40 in a physiological medium of viscosity η=1.0 cp. The sensitivity of the force sensor has been tested by imaging a MCF7 cell surface. © 2001 American Institute of Physics.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1063/1.1409586

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