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Crystallization and preliminary crystallographic analysis of two β-mannanase isoforms from Thermomonospora fusca KW3 (1996)

Hilge, M. ; Gloor, S. ; Winterhalter, K. ; [et al.]

Copenhagen : International Union of Crystallography (IUCr)

Acta crystallographica 52 (1996), S. 1224-1225

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ISSN: 1399-0047

Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001

Topics: Chemistry and Pharmacology , Geosciences , Physics

Notes: Three β-mannanase isoforms were isolated from the supernatant of a thermophilic actinomycete culture from Thermomonospora fusca KW3. Two of the isoforms (Q1, Q 1.1) were crystallized by the hanging-drop method at room temperature using ammonium sulfate as a precipitant. The isoforms form rod-shaped colorless crystals. Both belong to the orthorhombic space group P212121. The cell dimensions are a = 46.7, b = 61.1, and c = 128.2 Å for isoform Q1, and a = 43.8, b = 46.2, and c = 132.8 Å for isoform Q1.1. The asymmetric unit of either isoform contains one mannanase molecule. Native data have been collected to 2.2 Å resolution for Q1 and to 1.65 Å resolution for Q1.1 using synchrotron radiation.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1107/S0907444996009006

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