ISSN:
0741-0581
Keywords:
Ultrathin frozen sections
;
Electron microscopy
;
Immunocytochemistry
;
Cryobiology
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Natural Sciences in General
Notes:
A description of ultrathin frozen sectioning is presented. Small pieces of lightly-fixed but unembedded tissue are cryoprotected in 80% sucrose, mounted on chucks and frozen in liquid nitrogen. Frozen sections approximately 100 nm thick for electron microscopy are cut at about -90°C, using a cryosectioning unit mounted on a conventional ultramicrotome. Sections are picked up from the surface of the knife with a droplet of 80% sucrose, and are applied to membrane-coated EM grids at room temperature. The mounted sections are then used for EM immunocytochemistry. Essentially the same sectioning procedure can provide frozen sections 1 μm thick for light microscope immunocytochemistry.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jemt.1060020512
