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1.
PAPER CURRENT
An electrochemical peptide-based Ara h 2 antibody sensor fabricated on a nickel (II)-nitriloacetic acid self-assembled monolayer using a His-tagged peptide (2014)
Publication Date: 2014-04-23
Description: Publication date: Available online 21 April 2014 Source:Analytica Chimica Acta Author(s): Anita J. Zaitouna , Rebecca Y. Lai We report, for the first time, the use of a Ni(II)-nitriloacetic acid (NTA) self-assembled monolayer (SAM) in the fabrication of an electrochemical peptide-based (E-PB) sensor for detection of anti-Ara h 2 antibodies. We compared the performance of the sensor fabricated on a Ni(II)-NTA SAM using a His-tagged peptide with the sensor fabricated using the conventional approach via direct immobilization of a thiolated peptide. While both sensors responded only to the correct antibody in the presence of random antibodies, we observed differences between the sensors. Specifically, the detection limit of the His-tagged sensor was 1pM, significantly lower than the 200pM detection limit of the conventional thiolated sensor. More importantly, unlike our previously developed E-PB sensors, both sensors were found to be regenerable and reusable. The thiolated sensor can be readily regenerated using guanidine hydrochloride; whereas the His-tagged sensor can be regenerated by direct displacement of the His-tagged probes using Ni(II) ions. Overall, our results show that both approaches are well-suited for E-PB sensor fabrication; more importantly, specific sensor properties such as detection limit and dynamic range can be tuned by simply using a different probe immobilization method. Graphical abstract
Print ISSN: 0003-2670
Electronic ISSN: 1873-4324
Topics: Chemistry and Pharmacology
Published by Elsevier
2.
PAPER CURRENT
Enhanced electrochemiluminescence from luminol at carboxyl graphene for detection of alpha fetoprotein (2014)
Publication Date: 2014-04-23
Description: Publication date: Available online 21 April 2014 Source:Analytical Biochemistry Author(s): Xiaojian Li , Qingfang Guo , Wei Cao , Yueyun Li , Bin Du , Qin Wei In this paper, a novel sensitive electrochemiluminescence (ECL) immunosensor was constructed by carboxyl graphene (GR) for enhancing luminol-O2 system emission. Herein, carboxyl GR was used to enhance the ECL intensity of luminol which had excellent electron-transfer ability and good solubility. The sensing platform was constructed by depositing carboxyl GR on electrodes and immobilizing antibodies on the surface of carboxyl GR through amidation. The specific immunoreaction between alpha fetoprotein (AFP) and antibodies resulted in the decrease of ECL intensity and the intensity decreased linearly with AFP concentrations in the range of 5 pg·mL-1-14 ng·mL-1 with a detection limit of 2.0 pg·mL-1. The proposed immunosensor exhibits high specificity, good reproducibility and longtime stability. It may become a promising technique for protein detection.
Print ISSN: 0003-2697
Electronic ISSN: 1096-0309
Topics: Biology , Chemistry and Pharmacology
Published by Elsevier
3.
PAPER CURRENT
Comparison of NIR and ATR-IR spectroscopy for the determination of the antioxidant capacity of Primulae flos cum calycibus (2014)
Publication Date: 2014-04-23
Description: Anal. Methods, 2014, Accepted ManuscriptDOI: 10.1039/C4AY00669K, PaperStefan Alexander Schonbichler, Georg Friedrich Falser, Shah Hussain, Lukas Bittner, Gudrun Abel, Michael Popp, Gunther K Bonn, Christian HuckIn this study near-infrared (NIR) and attenuated-total-reflectance infrared (ATR-IR) spectroscopy in hyphenation with partial least squares (PLS) regression were utilized to determine the antioxidant capacity of Primulae flos cum calycibus...The content of this RSS Feed (c) The Royal Society of Chemistry
Print ISSN: 1759-9660
Electronic ISSN: 1759-9679
Topics: Chemistry and Pharmacology
4.
PAPER CURRENT
Improved Reagents for Newborn Screening of Mucopolysaccharidosis Types I, II, and VI by Tandem Mass Spectrometry (2014)
Publication Date: 2014-04-23
Description: Analytical ChemistryDOI: 10.1021/ac5004135
Print ISSN: 0003-2700
Electronic ISSN: 1520-6882
Topics: Chemistry and Pharmacology
5.
PAPER CURRENT
Quantitative Aspects of Normalized Differential Reflectance Spectroscopy: Pt(111) in Aqueous Electrolytes (2014)
Publication Date: 2014-04-23
Description: Analytical ChemistryDOI: 10.1021/ac403895z
Print ISSN: 0003-2700
Electronic ISSN: 1520-6882
Topics: Chemistry and Pharmacology
6.
PAPER CURRENT
Comparative Study of Sensitivity, Linearity, and Resistance to Inhibition of Digital and Nondigital Polymerase Chain Reaction and Loop Mediated Isothermal Amplification Assays for Quantification of Human Cytomegalovirus (2014)
Publication Date: 2014-04-23
Description: Analytical ChemistryDOI: 10.1021/ac500208w
Print ISSN: 0003-2700
Electronic ISSN: 1520-6882
Topics: Chemistry and Pharmacology
7.
PAPER CURRENT
Preconcentration of trace gold and silver on titanium dioxide nanotubes and their determination by inductively coupled plasma mass spectrometry (2014)
Publication Date: 2014-04-23
Description: Anal. Methods, 2014, Advance ArticleDOI: 10.1039/C4AY00353E, PaperShizhong Chen, Xinle Guo, Yan Zhang, Dengbo LuBased on titanium dioxide nanotubes (TDNTs) as a solid phase extraction adsorbent, a microcolumn preconcentration method coupled with inductively coupled plasma mass spectrometry was developed for the determination of trace gold and silver in environmental and biological samples.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry
Print ISSN: 1759-9660
Electronic ISSN: 1759-9679
Topics: Chemistry and Pharmacology
8.
PAPER CURRENT
A G-quadruplex DNAzyme chemiluminescence aptasensor based on the target triggered DNA recycling for sensitive detection of adenosine (2014)
Publication Date: 2014-04-23
Description: Anal. Methods, 2014, Advance ArticleDOI: 10.1039/C4AY00251B, PaperZhidan Chu, Liangliang Zhang, Yong Huang, Shulin ZhaoA G-quadruplex DNAzyme chemiluminescence aptasensor based on the target triggered DNA recycling was constructed for sensitive detection of adenosine.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry
Print ISSN: 1759-9660
Electronic ISSN: 1759-9679
Topics: Chemistry and Pharmacology
9.
PAPER CURRENT
Amplified fluorescence sensing of miRNA by combination of graphene oxide with duplex-specific nuclease (2014)
Publication Date: 2014-04-23
Description: Anal. Methods, 2014, Advance ArticleDOI: 10.1039/C4AY00345D, PaperShuang Guo, Fan Yang, Yulin Zhang, Yong Ning, Qunfeng Yao, Guo-Jun ZhangA graphene oxide-based fluorescent sensing system for sensitive detection of miRNA with a duplex-specific nuclease-aided signal amplification strategy.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry
Print ISSN: 1759-9660
Electronic ISSN: 1759-9679
Topics: Chemistry and Pharmacology
10.
PAPER CURRENT
A fluorescent sensor with high selectivity for Ca2+ against Mg2+ in seawater (2014)
Publication Date: 2014-04-23
Description: Anal. Methods, 2014, Advance ArticleDOI: 10.1039/C4AY00312H, CommunicationDaying Liu, Jing Qi, Zhiqiang Yu, Xiaoyan Liu, Renwei Yang, Haitao Yang, Hexi Chang, Huarui He, Guangming YangA new 4-amino-1,8-naphthalimide-based fluorescent sensor with phenyliminodiethoxyacetic acid as a receptor was synthesized and characterized. It can be used for continuous monitoring of the calcium ion concentration in seawater with high selectivity against magnesium ions.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry
Print ISSN: 1759-9660
Electronic ISSN: 1759-9679
Topics: Chemistry and Pharmacology