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  • Artikel  (853)
  • Triticum aestivum  (853)
  • Springer  (853)
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  • Springer  (853)
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  • 1
    Digitale Medien
    Digitale Medien
    Early signs of disruption of wheat anther development associated with the induction of male sterility by meiotic-stage water deficit (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 40-48 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Key words Male sterility ; Starch ; Triticum aestivum ; Water stress ; Anther ; Pollen
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Water deficit during meiosis in microspore mother cells of wheat (Triticum aestivum L.) induces male sterility, which reduces grain yield. In plants stressed during meiosis and then re-watered, division of microspore mother cells seems to proceed normally, but subsequent pollen development is arrested. Stress-affected anthers generally lack starch. We employed light microscopy in conjunction with histochemistry to compare the developmental anatomy of water-stress-affected and normal anthers. The earliest effects of stress, detectable between meiosis and young microspore stages, were the degeneration of meiocytes, loss of orientation of the reproductive cells, and abnormal vacuolization of tapetal cells. Other effects observed during subsequent developmental stages were deposition of starch in the connective tissue where it is normally not present, hypertrophy of the middle layer or endothecial cells, and deposition of sporopollenin-like substances in the anther loculus. The resulting pollen grains lacked both starch and intine. These results suggest that abnormal degeneration of the tapetum in water-stressed anthers coupled with a loss of orientation of the reproductive cells could be part of early events leading to abortion of microspores.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004970050066
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  • 2
    Digitale Medien
    Digitale Medien
    Gene transfer to inflorescence and flower meristems using ballistic micro-targeting (1994)
    Springer
    Sexual plant reproduction 7 (1994), S. 135-143 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Flower ; Meristem ; Gene transfer Particle bombardment ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Direct gene transfer to floral meristems could contribute to cell-fate mapping, to the study of flower-specific genes and promoters, and to the production of transgenic gametes via the transformation of sporogenic tissues. Despite the wide potential of its applications, direct gene transfer to floral meristems has not been achieved so far because of the lack of suitable technology. We show in this paper that ballistic micro-targeting is the technique of choice for this purpose, and in this way, we were able to transfer genes efficiently into excised wheat immature spikes. Particle size was adjusted for optimal penetration into the L1 and L2 cell layers of the spikes with limited cell damage. Spikes at different developmental stages were shot either with a plasmid containing two genes involved in anthocyanin biosynthesis or with a plasmid bearing the uidA (β-glucuronidase) gene. The transient expression of these marker genes was observed in the different developmental stages tested and in cells of both the L1 and the L2 layers. The transient expression of the uidA gene was significantly increased when the sucrose concentration in the culture medium was increased from 0.06 to 0.52 M. At the highest concentration, 100% of the targeted spikes expressed the uidA gene, with an average of 69 blue cells per spike. Twelve days after microtargeting, multicellular sectors showing transgene expression and containing up to 17 cells were found in 85% of the shot immature inflorescences. This indicated that targeted cells survived particle bombardment. Sectors were found in primordia of both vegetative and reproductive organs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00230582
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  • 3
    Digitale Medien
    Digitale Medien
    Accumulation of secondary compounds in barley and wheat roots in response to inoculation with an arbuscular mycorrhizal fungus and co-inoculation with rhizosphere bacteria (1999)
    Springer
    Mycorrhiza 8 (1999), S. 241-246 
    Details
    ISSN: 1432-1890
    Schlagwort(e): Key words Arbuscular mycorrhiza ; Hordeum vulgare ; Triticum aestivum ; Glomus intraradices ; Mycorrhiza-helper bacteria ; Secondary compounds
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Colonization of Hordeum vulgare L. cv. Salome (barley)and Triticum aestivum L. cv. Caprimus (wheat) roots by the arbuscular mycorrhizal fungus Glomus intraradices Schenck & Smith leads to de novo synthesis of isoprenoid cyclohexenone derivatives with blumenin [9-O-(2′-O-β-glucuronosyl)-β-glucopyranoside of 6-(3-hydroxybutyl)-1,1,5-trimethyl-4-cyclohexen-3-one] as the major constituent and to transient accumulation of hydroxycinnamate amides (4-coumaroylagmatine and -putrescine). Accumulation of these compounds in mycorrhizal wheat roots started 2 weeks after sowing together with the onset of arbuscule formation and proceeded with mycorrhizal progression. Highest levels were reached in 3- to 4-week-old secondary roots (root branches of first and higher order) characterized by the formation of vesicles. In the final developmental stages, the fungus produced massive amounts of spores, enclosing the stele of older root parts (older than 5 weeks) characterized by cortical death. In these root parts, the secondary compounds were detected in trace amounts only, indicating that they were located in the cortical tissues. Some rhizosphere bacteria tested, i.e. Agrobacterium rhizogenes, Pseudomonas fluorescens, and Rhizobium leguminosarum, markedly stimulated both fungal root colonization and blumenin accumulation, thus, acting as mycorrhiza-helper bacteria (MHB). Application of blumenin itself strongly inhibited fungal colonization and arbuscule formation at early stages of mycorrhiza development. This was associated with a markedly reduced accumulation of the hydroxycinnamate amides 4-coumaroylputrescine and -agmatine. The results suggest that both the isoprenoid and the phenylpropanoid metabolism are closely linked to the developmental stage and the extent of fungal colonization. Their possible involvement in the regulation of mycorrhiza development is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s005720050240
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  • 4
    Digitale Medien
    Digitale Medien
    Root respiratory quotient and nitrate uptake in hydroponically grown non-mycorrhizal and mycorrhizal wheat (1999)
    Springer
    Mycorrhiza 9 (1999), S. 57-60 
    Details
    ISSN: 1432-1890
    Schlagwort(e): Key words Glomus mosseae ; Hydroponics ; Nitrate uptake ; Root respiration ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Oxygen and CO2 fluxes were measured in hydroponically grown mycorrhizal and non-mycorrhizal Triticum aestivum L. cv. Hano roots. The NO3 – uptake of the plants was used to estimate the amount of root respiration attributable to ion uptake. Plants were grown at 4 mM N and 10 μM P, where a total and viable mycorrhizal root colonisation of 48% and 18%, respectively, by Glomus mosseae (Nicol. and Gerd.) Gerd. and Trappe (BEG 107) was observed. The O2 consumption and NO3 – uptake rates were similar and the CO2 release was higher in mycorrhizal than in non-mycorrhizal wheat. This resulted in a significantly higher respiratory quotient (RQ, mol CO2 mol–1 O2) in mycorrhizal (1.27±0.13) than in non-mycorrhizal (0.79±0.05) wheat. As the biomass and N and P concentrations in mycorrhizal and non-mycorrhizal wheat were the same, the higher RQ resulted from the mycorrhizal colonisation and not differences in nutrition per se.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s005720050263
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  • 5
    Digitale Medien
    Digitale Medien
    Embryogenic cell suspensions of Triticum aestivum x Leymus angustus F1 hybrids: characterization and plant regeneration (1992)
    Springer
    Plant cell reports 11 (1992), S. 81-85 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Cell suspension ; Somatic embryogenesis ; Triticum aestivum ; Leymus angustus ; Plant regeneration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Embryogenic cell suspension cultures were established from Triticum aestivum X Leymus angustus F1 hybrids, using compact nodular calli derived from inflorescence segments. Calli originating from leaf segments did not give rise to stable cell suspensions. Growth measurements of the cell suspensions revealed that they continued rapid growth up to 10 days after subculturing. Flow cytometric studies of the cell cycle over a 7 day culture period showed that the majority of cells were in G1 phase while the rest were either in S or G2. During the 7 days of culture, no significant differences in DNA distribution patterns were observed. The cells from suspension cultures produced somatic embryos when they were transferred to different solid media. The embryos germinated and gave rise to plantlets which were successfully rooted and transferred to soil.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00235258
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  • 6
    Digitale Medien
    Digitale Medien
    Plant regeneration from long term suspension culture-derived protoplasts of hexaploid wheat (Triticum aestivum L.) (1992)
    Springer
    Plant cell reports 11 (1992), S. 262-265 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Triticum aestivum ; embryogenic suspension cultures ; protoplasts ; regeneration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Highly regenerable callus cultures have been obtained from immature embryos of hexaploid wheat cv. Oderzo. Friable fast growing calli were induced at high frequency. Suspensions were initiated from the most friable callus lines: they became established in about two months. Suspensions consisted of cell aggregates of 30 to 1000 um in diameter. Upon plating on MS hormone-free medium, suspensions regenerated green plantlets, and their regenerative capability was maintained for at least 10 months. Protoplasts were isolated from 7–8 day old suspension cultures with a yield of 4–6×106 protoplasts/g fresh weight cells. Protoplast culture was either in liquid medium or in a bead-type system with agarose beads. First divisions were detected at day 5. At day 14 visible colonies were detected and the plating efficiency was evaluated between 2 and 8% over the initial number of protoplasts plated. Protoplast-derived calli were cultured in the presence of 1 mg/l IAA and 0.5 mg/l zeatin and were used for reinitiating new suspension cultures. Upon plating onto MS hormone-free medium, with or without the addition of 0.1 mg/l GA3, calliclones were induced to differentiate. Regeneration of complete plantlets, with shoot and roots took about two months. Plantlets were grown in sterile conditions until 12–15 cm height, and were subsequently transplanted in soil.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00235078
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  • 7
    Digitale Medien
    Digitale Medien
    Cryopreservation of immature spring wheat zygotic embryos using an abscisic acid pretreatment (1993)
    Springer
    Plant cell reports 12 (1993), S. 89-94 
    Details
    ISSN: 1432-203X
    Schlagwort(e): cryopreservation ; Triticum aestivum ; abscisic acid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Spring wheat (Triticum aestivum L.) zygotic embryos were successfully cryopreserved, without the addition of exogenous cryoprotectants, using only an abscisic acid (ABA) pretreatment. Optimum survival was obtained when embryos were cultured in vitro for 10 days on semisolid Murashige and Skoog (MS) nutrient medium supplemented with 0.5 mg/L (±) ABA prior to cryopreservation. The embryos resumed growth within three days when returned to MS medium devoid of ABA but containing 2mg/L 2,4-dichlorophenoxyacetic acid. The embryogenic calli produced from these embryos exhibited normal plant regeneration on auxin-free media. Changes in dw/fw ratio, as well as the esterified fatty acid and sucrose concentrations correlated positively with the development of tolerance to cryopreservation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00241941
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  • 8
    Digitale Medien
    Digitale Medien
    Plant regeneration from isolated microspores of Triticum aestivum (1993)
    Springer
    Plant cell reports 12 (1993), S. 149-153 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Triticum aestivum ; microspore culture ; ovary co-culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Wheat microspores were isolated, without prior anther culture, from a range of genotypes and cultured to regenerate self-fertile plants. Microspores were isolated using a microblender and competent microspores were enriched by gradient centrifugation. The use of maltose as the sole carbohydrate in the culture medium and co-culture of microspores with wheat or barley ovaries were critical for development of microspore-derived embryos. Results were also improved when spikes were pretreated by emersion of the basal ends of detached heads in water at 25°C for 2d. This procedure leads to highly reproducible production of plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00239096
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  • 9
    Digitale Medien
    Digitale Medien
    In vitro development of wheat (Triticum aestivum L.) from zygote to plant via ovule culture (1997)
    Springer
    Plant cell reports 16 (1997), S. 663-667 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key words Embryogenesis ; Ovule culture ; Triticum aestivum ; Zygote
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Ovules of the wheat breeding line Veery #5 were excised and transferred to culture within 24 h after pollination. When ovules were cultured on Phytagel-solidified medium, and the pericarp removed exclusively at the micropylar tip and the abaxial side, zygotes from up to 79.2% of the ovules underwent embryogenesis with the same developmental pattern as found in planta. Embryos from more than 50% of the cultured ovules germinated when transferred to regeneration medium. More than 100 plantlets were randomly chosen for transfer to soil, all of which developed to phenotypically normal and fertile plants. With this system, the entire process of zygotic embryogenesis can be studied using living material. Furthermore, the method could be used as an embryo rescue technique for plant breeding purposes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990050298
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  • 10
    Digitale Medien
    Digitale Medien
    Effects of light on the accumulation of abscisic acid and expression of an early cysteine-labeled metallothionein gene in microspores of Triticum aestivum during induced embryogenic development (1997)
    Springer
    Plant cell reports 16 (1997), S. 458-463 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key words Abscisic acid ; Anther culture ; Light ; Metallothionein ; Pollen embryogenesis ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A cloned cDNA to the wheat (Triticum aestivum) early cysteine-labeled metallothionein has many characteristics of a molecular marker for pollen embryogenesis in this plant. This transcript was not detected in uninucleate microspores at the time of culture or in pollen at any stage during normal ontogeny; its mRNA did begin to increase in embryogenic microspores within 6 h of culture, peaked at around 24 h, declined, then leveled off through the 21-day-old embryoid stage. Additionally, the accumulation of the embryoid-abundant EcMt gene transcript showed a direct and positive correlation with an increase of ABA in embryogenic microspores and developing pollen embryoids. Irradiating cultures with high intensity white light or with far-red, or blue light, suppressed EcMt transcript accumulation and the ability of microspores to form embryoids; however, light did not affect ABA concentrations during the early stages of culture. These results suggest that although a promoter of pollen embryogenesis in bread wheat, ABA alone cannot maintain the sporophytic differentiation of microspores subjected to inhibitory regimes of light in vitro. Whether or not light acts directly or indirectly in suppressing EcMt gene expression and pollen embryogenesis remains unknown.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01092766
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  • 11
    Digitale Medien
    Digitale Medien
    The pattern of amyloplast DNA accumulation during wheat endosperm development (1987)
    Springer
    Planta 171 (1987), S. 416-421 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Amyloplast DNA ; DNA accumulation ; Endosperm development ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The accumulation of amyloplast DNA during endosperm development was studied in two cultivars of spring wheat, Triticum aestivum L. ‘Chinese Spring’ (CS) and ‘Spica’, small and relatively larger-grained cultivars, respectively. Endosperms were isolated between 9 and 45 days post anthesis (dpa) and the amyloplast DNA content of endosperm nucleic-acid extracts was measured by quantitative hybridisation with a homologous chloroplast-DNA probe. The endosperm cells of CS and Spica accumulated amyloplast DNA during development in a similar way. In both cultivars there was a large increase in the amount of plastid DNA (ptDNA) per endosperm between 9 and about 15 dpa, after which there was no further increase. Because nuclear DNA continued to accumulate until 24 dpa, the percentage contribution of amyloplast DNA to total DNA fluctuated in both cultivars during development, reaching maxima at 12 dpa of about 1.00% and 0.85%, and dropping to apparently constant levels of 0.60% and 0.52% in CS and Spica, respectively, by 24 dpa. In both cultivars, the average number of ptDNA copies per amyloplast was calculated to increase from about 10 copies at 9 dpa to about 50 copies in the mature amyloplasts at 31 dpa. However, the heavier endosperms of Spica contain more cells than those of CS and the varieties therefore differed in the amount of ptDNA that accumulated per endosperm: Spica endosperms accumulated 110 ng of ptDNA by 15 dpa, compared with only 85 ng in CS. The apparent accumulation of ptDNA copies in wheat amyloplasts during endosperm development contrasts with the decline in chloroplast-DNA copies in wheat chloroplasts during leaf development.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00398688
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  • 12
    Digitale Medien
    Digitale Medien
    Increasing grain protein content of hard red winter wheat (Triticum aestivum L.) by mutation breeding (1983)
    Springer
    Theoretical and applied genetics 65 (1983), S. 41-46 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Wheat ; Protein ; Mutation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Poor adaptability or functional quality of much germplasm used for breeding high-protein hard red winter wheats prompted mutagenesis as an alternative means of increasing grain protein content. Four hard red winter wheat genotypes — KS644 (‘Triumph// Concho/Triumph’), ‘Kaw’, ‘Parker’, and ‘Shawnee’ — were treated with 0.40 M ethyl methanesulfonate (EMS). Advanced lines (M8-M10) were selected that had a 3-year mean grain protein advantage of 0.7% to 2.0% over controls. Increased grain protein content was generally associated with decreased grain yield and kernel weight, but some high-protein mutant lines had yields or kernel weights similar to those of original genotypes. Changes in height and lodging induced by EMS were generally favorable, most mutants being shorter and lodging less than controls, but blooming date was generally delayed, a deleterious change. One line also changed from resistant to segregating for wheat soil-borne mosaic virus. Mutant lines might be utilized in cross-breeding programs, particularly if negative pleiotropic effects and linkages are absent.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00276260
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  • 13
    Digitale Medien
    Digitale Medien
    Protein accumulation, RNA and soluble amino nitrogen content in developing endosperm of two varieties of Triticum aestivum with high and low protein seed (1977)
    Springer
    Theoretical and applied genetics 50 (1977), S. 73-77 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Wheat ; Endosperm ; Protein Synthesis ; RNA Level ; Amino Acid Translocation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The kinetics of protein accumulation, the variation in RNA, the soluble amino nitrogen content of developing endosperm of two varieties of Triticum aestivum, with high and low protein content in the mature seed, suggest a possible relation between maintenance of the RNA content and the ability to synthesize protein. A sudden halt in protein accumulation is observed as the RNA starts to decrease. The hypothesis is also advanced that maintenance of the RNA content might, in turn, be dependent on the presence, in the endosperm of developing wheat seed, of a certain level of soluble amino nitrogen which could then play the role of limiting factor for protein synthesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00277249
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  • 14
    Digitale Medien
    Digitale Medien
    The peroxidase isozymes of the wheat kernel: tissue and substrate specificity and their chromosomal location (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 701-706 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Peroxidases ; Tissue and substrate-specificity ; Chromosomal location
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Peroxidase isozymes were studied in the Triticum aestivum L. kernel and in nullisomic-tetrasomic and ditelocentric combinations of ‘Chinese Spring’ wheat. Analyses were carried out on different parts of dry kernels (embryo plus scutellum and endosperm) using polyacrylamide and starch gel electrophoresis, different electrophoretic buffer systems and various staining methods. The peroxidase isozymes showed a low substrate-specificity and a high tissue-specificity. The embryo plus scutellum and the endosperm always presented different peroxidase patterns. Endosperm peroxidases were associated with chromosome arms 7DS, 4BL and 7AS; whereas the embryo plus scutellum isozymes were related to chromosome arms 3AL, 3BL and 3DS. The different results obtained using various electrophoretic techniques are due to the buffer system used. All staining procedures employed revealed the same peroxidase isozymes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00260779
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  • 15
    Digitale Medien
    Digitale Medien
    Extent of genetic variability of endosperm esterases in Triticum aestivum L. 2n=6x=42 (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 728-734 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Esterases ; Genetic variability ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Genetic variability of endosperm esterase has been studied in 42 cultivars of Triticum aestivum L. 2n=6x=42. Different techniques, including sequential electrophoresis and electrofocusing, have been used with various substrates and esterase inhibitors. The electrophoretic patterns in each cultivar are described. Chromosomal location using the nullitetrasomic and ditelosomic lines of Chinese Spring was carried out in order to relate and/or locate the esterase genes to specific chromosomes. Most of the esterase isozymes located were in the long arm of the chromosomes of the homoeology group 3; but we have found six located in the short arms, five of them in the chromosome 3AS and one in the 3DS. This location increases the number of esterase genes described, because no esterase genes had been described so far in short arms of chromosomes of the homoeology group 3. The genetic control is discussed and, according to our results, between 12 and 15 loci, organized in five “compound loci”, control the endosperm esterases in wheat. Also one “modifier” gene modifying the mobility of two esterase bands and present in all the cultivars studied is postulated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00262570
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  • 16
    Digitale Medien
    Digitale Medien
    Hybridization between Triticum aestivum L. and Agropyron michnoi Roshev. (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 312-316 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Agropyron michnoi ; Intergeneric hybrid ; Chromosome pairing ; Self-fertile
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Intergeneric hybrids between Triticum aestivum cv ‘Chinese Spring’ (2n=6x=42, AABBDD) and Agropyron michnoi Roshev. (2n=4x=28, PPPP) were obtained by embryo culture. Their spike characteristics were similar to those of common wheat but, unlike their parents, they were long-awned. The average meiotic chromosome pairing at MI of F1 hybrids was: 6.39 I +3.75 rodII+8.64 ringII+0.81 III+0.30 IV+0.04 V, the bivalent and multivalent formation of which was much higher than expected from the genomic formulae. It is especially worthwhile to note that the F1 hybrids were self-fertile, self set being 0.15%, and seeds were easily obtained from the backcross of f1 plants with hexaploid and tetraploid wheats; here the seed set was more than 20.0%. The polyploid taxa and the position of A. Michnoi in Agropyron are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228669
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  • 17
    Digitale Medien
    Digitale Medien
    Determination of the transmission frequency of chromosome 4S l of Aegilops sharonensis in a range of wheat genetic backgrounds (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 519-523 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Preferential transmission ; Triticum aestivum ; Aegilops sharonensis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The transmission of chromosome 4S l from Aegilops sharonensis was observed in a range of wheat genetic backgrounds. Chromosome 4S l was transmitted at a very high frequency (at least 97.8%) in all crosses. The genetic background appears to only have a small effect on transmission. The frequency of transmission of chromosome 4S l was the same in each genetic background through both the male and female gametes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219443
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  • 18
    Digitale Medien
    Digitale Medien
    Seed set after pollination with in-vitro-matured, isolated pollen of Triticum aestivum (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 576-580 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Pollen culture ; Pollen maturation ; Pollination ; Triticum aestivum ; Gliadins
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Immature pollen of two varieties of Triticum aestivum, at the stage right after the first pollen mitosis, was isolated from individual anthers and cultured in microcultures of microliter droplets. In a specifically designed medium, some of the pollen grains developed to maturity. These were applied to excised stigmas on agar, where they produced pollen tubes. Application to flowers in vivo led to seed set. Pollen was matured in vitro from a variety that produced a different protein banding pattern on SDS-PAGE as compared to the variety that was pollinated. The protein banding in the produced seeds showed the hybrid pattern, demonstrating that the seeds were not produced by self-pollination in this in-breeding species but by pollination with the in-vitro-matured pollen.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226721
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  • 19
    Digitale Medien
    Digitale Medien
    Dosage response of rye genes in a wheat background (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 1-5 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Secale cerale ; Alien gene expression ; Endosperm protein
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A series of hexaploid wheat lines containing zero, two or four doses of rye chromosome arm 1RS was used to investigate the response to changes in dosage by the rye genes when in a wheat background. The quantity of protein produced by the secalin protein genes contained on 1RS was directly related to the number of copies of 1RS present in the line. No response could be detected by representative wheat proteins suggesting that the increase in secalin protein observed was due to an increase in mRNA produced when four copies of the secalin gene was present. These results suggest that increasing the dosage of alien genes introgressed into wheat may be a useful tool for enhancing their expression. Mention of a trade name or proprietary product does not constitute a guarantee, warranty or recommendation of the product by the U.S. Department of Agriculture or the University of Missouri and does not imply its approval to the exclusion of other products that may be suitable.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223973
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  • 20
    Digitale Medien
    Digitale Medien
    Production and cytogenetic analysis of BC1, BC2, and BC3 progenies of an intergeneric hybrid between Triticum aestivum (L.) Thell. and tetraploid Agropyron cristatum (L.) Gaertn. (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 698-703 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Agropyron ; Intergeneric hybrids ; Backcross derivatives ; Chromosome pairing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Intergeneric hybrids between Triticum aestivum cv ‘Chinese Spring’ and Agropyron cristatum 4x (2n= 5x=35, ABDPP genomes) with a high level of homoeologous meiotic pairing between the wheat chromosomes were backcrossed 3 times to wheat. Pollination of the F1 hybrid with ‘Chinese Spring’ resulted in 22 BC1 seeds with an average seed set of 1.52%. Five BC1 plants with 39–41 chromosomes were raised using embryo rescue techniques. Chromosome pairing in the BC1 was characterized by a high frequency of multivalent associations, but in spite of this there was no evidence of homoeologous pairing between chromosomes of wheat and those of Agropyron. All of the plants were self sterile. The embryo rescue technique was again essential to produce 39 BC2 plants with chromosome numbers ranging from 37 to 67. The phenomenon of meiotic non-reduction was also observed in the BC3 progenies. In this generation male and female fertility greatly increased, and meiotic pairing was fairly regular. Some monosomic (2n=43) and double monosomic (2n=44) lines were produced. Analysis of these progenies should permit the extraction of the seven possible wheat-Agropyron disomic addition lines including those with the added chromosomes carrying the genes involved in meiotic non-reduction and in suppression of Ph activity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224171
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  • 21
    Digitale Medien
    Digitale Medien
    Differences in the heat-shock response between thermotolerant and thermosusceptible cultivars of hexaploid wheat (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 941-946 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Genetic differences ; Heat-shock proteins ; Heat-shock response ; DNA polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Heat-shock protein (HSP) gene expression in two wheat lines cv ‘Mustang’ (heat-tolerant) and cv ‘Sturdy’ (heat-susceptible) were analyzed to determine if wheat genotypes differing in heat tolerance also differ in in-vitro HSP synthesis (translatable HSP mRNAs) and steady-state levels of HSP mRNA. Several sets of mRNA were isolated from seedling leaf tissues which had been heat-stressed at 37 °C for various time intervals. These mRNAs were hybridized with HSP cDNA or genomic DNA probes (HSP17, 26, 70, 98, and ubiquitin). Protein profiles were compared using in-vitro translation and 2-D gels. The Northern slot-blot data from the heat-stress treatment provide evidence that the heat-tolerant cv ‘Mustang’ synthesized low molecular weight (LMW) HSP mRNA earlier during exposure to heat shock and at a higher level than did the heat-susceptible cv ‘Sturdy’. This was especially true for the chloroplast-localized HSP. The protein profiles shown by 2-D gel analysis revealed that there were not only quantitative differences of individual HSPs between the two wheat lines, but also some unique HSPs which were only found in the ‘Mustang’ HSP profiles. The high level of RFLP between the two wheat lines was revealed by Southern blot hybridization utilizing a HSP17 probe. These data provide a molecular basis for further genetic analysis of the role of HSP genes in thermal tolerance in wheat.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00227407
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  • 22
    Digitale Medien
    Digitale Medien
    Production of multiple wheat-rye 1RS translocation stocks and genetic analysis of LMW subunits of glutenin and gliadins in wheats using these stocks (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 719-728 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Rye translocations ; Triticum aestivum ; Glutenin ; Gliadin ; Glu-3 loci ; Gli-1 loci
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A triple (1AL.1RS/1BL.1RS/1DL.1RS) and three double (1AL.1RS/1BL.1RS, 1AL.1RS/1DL.1RS, 1BL.1RS/1DL.1RS) wheat-rye 1RS translocation stocks were isolated from a segregating population using the Gli-1, Tri-1 and Sec-1 seed proteins as genetic markers. These stocks carried 42 chromosomes and formed the expected multivalents (frequency of 14–25%) at metaphase 1. They gave floret fertility ranging from 40–60%. These stocks were subsequently used to determine the genetic control of low-molecular-weight (LMW) glutenin subunits in ‘Chinese Spring’ and ‘Gabo’ by means of two-step one-dimensional SDS-PAGE. All of the B subunits and most of the C subunits of glutenin were shown to be controlled by genes on the short arms of group-1 chromosomes in these wheats. The other C subunits were not controlled by group-1 chromosomes. The triple translocation line served as a suitable third parent in producing test-cross seeds for studying the inheritance of the LMW glutenin subunits and gliadins in wheat cultivars, e.g. ‘Chinese Spring’ and ‘Orca’. The segregation patterns of the LMW glutenin subunits in these cultivars revealed that the subunits were inherited in clusters and that their controlling genes (Glu-3) were tightly linked with those controlling gliadins (Gli-1). The LMW glutenin patterns d, d and e in ‘Orca’ segregated as alternatives to the patterns a, a and a in ‘Chinese Spring’ controlled by Glu-A3, Glu-B3 and Glu-D3 loci on chromosome arms 1AS, 1BS and 1DS, respectively, thus indicating that these patterns were controlled by allelic genes at these loci.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00225011
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  • 23
    Digitale Medien
    Digitale Medien
    Restriction fragment patterns of chloroplast and mitochondrial DNA of Dasypyvum villosum (L.) candargy and wheats (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 44-48 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; T. durum ; Aegilops longissimum ; Dasypyrum villosum ; Endonuclease ; Cytoplasm donor
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract To elucidate the phylogenetic relationships and cytoplasmic types, restriction endonuclease fragment patterns of chloroplast (cp) and mitochondrial (mt) DNAs isolated from two different accessions of Dasypyrum villosum (L.) candargy were compared with those of tetraploid wheat (Triticum durum Desf., PI265007), hexaploid wheat (Triticum aestivum L., cv Chinese Spring), Aegilops longissimum (S. and M., in Muschli) Bowden and Hordeum vulgare L. T. aestivum and T. durum had identical restriction patterns for their cp and mtDNAs in digestions with four different enzymes. Likewise, no differences were found between the restriction fragment patterns of two accessions of D. villosum. But, there were distinct differences in chloroplast and mitochondrial DNA restriction fragment patterns between D. villosum and tetraploid and hexaploid wheats. A. longissimum (G609) showed a similar pattern to those wheats for PstI digestion of cpDNA. Organellar DNA from Hordeum vulgare (cv Himalaya) showed a distinctly different restriction pattern from those of wheat and D. villosum. These results suggest that D. villosum is unlikely to be the donor of cytoplasm to wheats, and its cytoplasmic organelles were also different from those of A. longissimum.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223742
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  • 24
    Digitale Medien
    Digitale Medien
    Use of RFLP markers for the identification of alleles of the Pm3 locus conferring powdery mildew resistance in wheat (Triticum aestivum L.) (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 959-963 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Mildew resistance ; Pm3 locus ; Near-isogenic lines ; RFLP marker
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The objective of this study was to identify molecular markers linked to genes for resistance to powdery mildew (Pm) in wheat using a series of ‘Chancellor’ near-isogenic-lines (NILs), each having one powdery mildew resistance gene. A total of 210 probes were screened for their ability to detect polymorphism between the NILs and the recurrent parent. One of these restriction fragment length polymorphism (RFLP) markers (Xwhs179) revealed polymorphism not only between the NILs for the Pm3 locus, but also among NILs possessing different alleles of the Pm3 locus. The location of the marker Xwhs179 was confirmed to be on homoeologous chromosome group 1 with the help of nullitetrasomic wheat lines. The linkage relationship between this probe and the Pm3 locus was estimated with double haploid lines derived from a cross between wheat cvs ‘Club’ and ‘Chul’ (Pm3b). The genetic distance was determined to be 3.3±1.9 cM.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00211048
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  • 25
    Digitale Medien
    Digitale Medien
    Transfer of Ph I genes promoting homoeologous pairing from Triticum speltoides to common wheat (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 97-101 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; T. speltoides ; Meiotic chromosome pairing ; Alien transfer
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Diploid-like chromosome pairing in polyploid wheat is controlled by several Ph (pairing homoeologous) genes with major and minor effects. Homoeologous pairing occurs in either the absence of these genes or their inhibition by genes from other species (Ph I genes). We transferred Ph I genes from Triticum speltoides (syn Aegilops speltoides) to T. aestivum, and on the basis of further analysis it appears that two duplicate and independent Ph I genes were transferred. Since Ph I genes are epistatic to the Ph genes of wheat, homoeologous pairing between the wheat and alien chromosomes occurs in the F1 hybrids. Using the Ph I gene stock, we could demonstrate homoeologous pairing between the wheat and Haynaldia villosa chromosomes. Since homoeologous pairing occurs in F1 hybrids and no cytogenetic manipulation is needed, the Ph I gene stock may be a versatile tool for effecting rapid and efficient alien genetic transfers to wheat.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222400
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  • 26
    Digitale Medien
    Digitale Medien
    Waxy protein deficiency and chromosomal location of coding genes in common wheat (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 179-184 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Waxy (Wx) protein ; Triticum aestivum ; Null allele ; Geographical distribution ; Chromosomal location
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Deficiency of the wheat waxy (Wx) proteins (Wx-A1, Wx-B1 and Wx-D1) was studied in 1,960 cultivars derived from several countries. Gel electrophoretic analyses revealed that the null allele for the Wx-A1 protein occurred frequently in Korean, Japanese and Turkish wheats but was relatively rare in cultivars from other countries and regions. About 48% of the wheats deficient for the Wx-B1 protein were from Australia and India. One Chinese cultivar lacked the WxD1 protein. While 9 Japanese cultivars were deficient in both the Wx-A1 and Wx-B1 proteins, no cultivars lacked both the Wx-A1 and Wx-D1 proteins, both the Wx-B1 and Wx-D1 proteins or all three Wx proteins. Two-dimensional gel electrophoresis revealed polymorphisms of the three Wx proteins that varied according to isoelectric points or molecular weight. The Wx-A1 gene coding the Wx-A1 protein and the Wx-B1 gene coding the Wx-B1 protein were localized in the distal regions of chromosome arms 7AS and 4AL, respectively, by deletion mapping using the deletion lines developed in the common wheat cultivar ‘Chinese Spring’.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00225138
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  • 27
    Digitale Medien
    Digitale Medien
    Comparison of wheat physical maps with barley linkage maps for group 7 chromosomes (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 618-626 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Comparative maps ; Deletion lines ; Molecular-tagged chromosome regions (MTCRs) ; Triticum aestivum ; Hordeum vulgare
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Comparative genetic maps among the Triticeae or Gramineae provide the possibility for combining the genetics, mapping information and molecular-marker resources between different species. Dense genetic linkage maps of wheat and barley, which have a common array of molecular markers, along with deletion-based chromosome maps of Triticum aestivum L. will facilitate the construction of an integrated molecular marker-based map for the Triticeae. A set of 21 cDNA and genomic DNA clones, which had previously been used to map barley chromosome 1 (7H), were used to physically map wheat chromosomes 7A, 7B and 7D. A comparative map was constructed to estimate the degree of linkage conservation and synteny of chromosome segments between the group 7 chromosomes of the two species. The results reveal extensive homoeologies between these chromosomes, and the first evidence for an interstitial inversion on the short arm of a barley chromosome compared to the wheat homoeologue has been obtained. In a cytogenetically-based physical map of group 7 chromosomes that contain restriction-fragment-length polymorphic DNA (RFLP) and random amplified polymorphic DNA (RAPD) markers, the marker density in the most distal third of the chromosome arms was two-times higher than in the proximal region. The recombination rate in the distal third of each arm appears to be 8–15 times greater than in the proximal third of each arm where recombination of wheat chromosomes is suppressed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223288
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  • 28
    Digitale Medien
    Digitale Medien
    Plant regeneration from immature embryos of 48 elite CIMMYT bread wheats (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 163-169 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Bread wheat ; Triticum aestivum ; Culture medium ; Embryogenic callus ; Plant regeneration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Forty-eight bread wheat (Triticum aestivum L.) released cultivars and elite advanced lines were evaluated for their ability to produce embryogenic callus using three different media. Basal N6 medium supplemented with dicamba (E1), MS medium containing 2,4-D (E3) or MS medium containing 2,4-D plus different amino acids (E5) were used for callus initiation and maintenance. Plant regeneration was achieved on basal MS medium with indole-3-acetic acid (IAA) and 6-benzylamino purine (BAP) and rooting on MS with 1-naphthaleneacetic acid (NAA). Percentage regeneration varied widely with both genotype and initiation medium, with values ranging from 2% to 94%. The number of plantlets produced per embryo ranged from 6 to 42. Thirteen genotypes showed at least 50% regeneration after culture on E5 medium; 3 genotypes after culture on E3 initiation medium and 1 after initiation on E1. After four subcultures, over a 16-week period, 41 genotypes (85%) lost their ability to regenerate plants while the remaining 7 lines (15%) retained plant regeneration potential but at reduced levels. E3 medium was found to be the best for maintaining regeneration potential after four subcultures.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223371
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  • 29
    Digitale Medien
    Digitale Medien
    Isolation and identification of Triticum aestivum L. em. Thell. cv Chinese Spring-T. peregrinum Hackel disomic chromosome addition lines (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 591-598 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words  Wheat ; Triticum aestivum ; Triticum peregrinum ; Chromosome addition lines ; RFLPs
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract   Analyses of RFLPs, isozymes, morphological markers and chromosome pairing were used to isolate 12 Triticum aestivum cv Chinese Spring (genomes A, B, and D)-T. peregrinum (genomes Sv and Uv) disomic chromosome addition lines. The evidence obtained indicates that each of the 12 lines contains an intact pair of T. peregrinum chromosomes. One monosomic addition line, believed to contain an intact 6Sv chromosome, was also isolated. A CS-7Uv chromosome addition line was not obtained. Syntenic relationships in common with the standard Triticeae arrangement were found for five of the seven Sv genome chromosomes. The exceptions were 4Sv and 7Sv. A reciprocal translocation exists between 4Sl and 7Sl in T. longissimum and evidence was obtained that the same translocation exists in T. peregrinum. In contrast, evidence for syntenic relationships in common with the standard Triticeae arrangements were found for only one Uv chromosome of T. peregrinum; namely, chromosome 2Uv. All other Uv genome chromosomes are involved in at least one translocation, and the same translocations were found in the U genome of T. umbellulatum. Evidence was also obtained indicating that the centromeric regions of 4U and 4Uv are homoeologous to the centromeric regions of Triticeae homoeologous group-6 chromosomes, that the centromeric regions of 6U and 6Uv are homoeologous to the centromeric regions of group-4 chromosomes, and that 4U and 4Uv are more closely related overall to Triticeae homoeologous group-6 chromosomes than they are to group-4 chromosomes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050168
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  • 30
    Digitale Medien
    Digitale Medien
    Characterization of spelt (Triticum spelta L.) forms by gel-electrophoretic analyses of seed storage proteins. III. Comparative analyses of spelt and Central European winter wheat (Triticum aestivum L.) cultivars by SDS-PAGE and acid-PAGE (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1340-1346 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Triticum spelta ; Triticum aestivum ; SDS-PAGE ; Acid-PAGE ; Seed storage proteins
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Seed storage proteins of a few selected spelt forms and crosses have already been electrophoretically analysed by SDS-PAGE and acid-PAGE and compared with a few winter wheat cultivars. In the analyses presented here further important Central European spelt varieties were included, as well as modern winter wheat cultivars which were chosen as standards. In this study gliadin and glutenin band patterns of modern Central European winter wheat cultivars were analysed, in particular for a comparison with spelt varieties. An improved differentiation within and between the two species was obtained.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051027
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  • 31
    Digitale Medien
    Digitale Medien
    Molecular cytogenetic characterization of Thinopyrum intermedium-derived wheat germplasm specifying resistance to wheat streak mosaic virus (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 1-7 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Fluorescence in situ hybridization ; Translocation ; WSMV resistance ; Thinopyrum intermedium ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract   Thinopyrum intermedium is a promising source of resistance to wheat streak mosaic virus (WSMV), a devastating disease of wheat. Three wheat germplasm lines possessing resistance to WSMV, derived from Triticum aestivum×Th. intermedium crosses, are analyzed by C-banding and genomic in situ hybridization (GISH) to determine the amount and location of alien chromatin in the transfer lines. Line CI15092 was confirmed as a disomic substitution line in which wheat chromosome 4A was replaced by Th. intermedium chromosome 4Ai?2. The other two lines, CI17766 and A29-13-3, carry an identical Robertsonian translocation chromosome in which the complete short arm of chromosome 4Ai?2 was transferred to the long arm of wheat chromosome 4A. Fluorescence in situ hybridization (FISH) using ABD genomic DNA from wheat as a probe and S genomic DNA from Pseudoroegneria stipifolia as the blocker, and vice versa, revealed that the entire short arm of the translocation was derived from the short arm of chromosome 4Ai?2 and the breakpoint was located at the centromere. Chromosomal arm ratios (L/S) of 2.12 in CI17766 and 2.15 in A29-13-3 showed that the translocated chromosome is submetacentric. This translocated chromosome is designated as T4AL ⋅ 4Ai?2S as suggested by Friebe et al. (1991).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050701
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  • 32
    Digitale Medien
    Digitale Medien
    The application of wheat microsatellites to identify disomic Triticum aestivum-Aegilops markgrafii addition lines (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 138-146 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Aegilops markgrafii ; Triticum aestivum ; Addition lines ; Chromosome markers ; Homoeology ; Wheat ; Wheat microsatellites
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  We describe the use of wheat microsatellites for the discrimination of Aegilops markgrafii chromosomes. Twenty out of eighty eight wheat microsatellites (WMS) tested were able to distinguish Triticum aestivum-Ae. markgrafii addition lines. Six, three, three, one and six of 18 WMS can be used as markers for single Ae. markgrafii chromosomes B, C, D, F and G, respectively. Addition line A is not available but additional bands, appearing only in Ae. markgrafii and the T. aestivum-Ae. markgrafii amphiploid and not in any of the available addition lines, indicate that three WMS detect markers for Ae. markgrafii chromosomes A. Addition line E could not be detected by any of the WMS markers applied, although the 20 WMS represented all the homologous groups of wheat. All three WMS located on the short arm of group-2 chromosomes were located on Ae. markgrafii chromosome B; three of four WMS, located on the long arm of wheat group-2 chromosomes, were specific to Ae. markgrafii chromosome G and three of four WMS, specific to group-5 chromosomes, were markers for Ae. markgrafii chromosome C, indicating the homoeology of these wheat chromosome arms with the respective Ae. markgrafii chromosomes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050720
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  • 33
    Digitale Medien
    Digitale Medien
    A high-density genetic linkage map of Aegilops tauschii, the D-genome progenitor of bread wheat (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 16-26 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Aegilops tauschii ; Triticum aestivum ; Genetic mapping ; Molecular markers ; Agronomically important genes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Aegilops tauschii is the diploid D-genome progenitor of bread wheat (Triticum aestivum L. em Thell, 2n=6x=42, AABBDD). A genetic linkage map of the Ae. tauschii genome was constructed, composed of 546 loci. One hundred and thirty two loci (24%) gave distorted segregation ratios. Sixty nine probes (13%) detected multiple copies in the genome. One hundred and twenty three of the 157 markers shared between the Ae. tauschii genetic and T. aestivum physical maps were colinear. The discrepancy in the order of five markers on the Ae. tauschii 3DS genetic map versus the T. aestivum 3D physical map indicated a possible inversion. Further work is needed to verify the discrepancies in the order of markers on the 4D, 5D and 7D Ae. tauschii genetic maps versus the physical and genetic maps of T. aestivum. Using common markers, 164 agronomically important genes were assigned to specific regions on Ae. tauschii linkage, and T. aestivum physical, maps. This information may be useful for map-based cloning and marker-assisted plant breeding.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051204
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  • 34
    Digitale Medien
    Digitale Medien
    Hessian fly-resistance gene transferred from chromosome 4Mv of Aegilops ventricosa to Triticum aestivum (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 858-864 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Aegilops ventricosa ; Triticum aestivum ; Mayetiola destructor ; Hessian fly ; Resistance gene
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A new Hessian fly (Mayetiola destructor) resistance gene from Aegilops ventricosa and its transfer to hexaploid wheat is described. The 4D(4Mv) substitution line H-93-33 derived from the cross [(Triticum turgidum H-1-1×Aegilops ventricosa no. 11)×Triticum aestivum H-10-15] was highly resistant to the Spanish population tested. Resistance seemed to be inherited as a single dominant factor in the F2 generation resulting from a cross of H-93-33 with its susceptible parent (H-10-15). Resistance in Ae. venticosa no. 10 was located on chromosome 4Mv using Mv wheat/Ae. ventricosa addition lines. The resistance gene transferred from Ae. ventricosa no. 11 to H-93-33 (H27) is allelic with respect to that of Ae. ventricosa no. 10 and is non-allelic with respect to the genes H3 and H6 from Monon and Caldwell respectively. The assignment of H27 gene to chromosome 4Mv is further supported by its linkage to a gene encoding isozyme Acph-Mv1, previously located on chromosome 4Mv in the line H-93-33. A new marker from homoeologous chromosome group 4 (Amp-Mv2) present in H-93-33 and the 4Mv addition line is described.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050487
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  • 35
    Digitale Medien
    Digitale Medien
    RAPDs as molecular markers for the detection of Aegilops markgrafii chromatin in addition and euploid introgression lines of hexaploid wheat (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 934-940 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Aegilops markgrafii ; Triticum aestivum ; RAPD ; Addition lines ; Leaf rust ; Powdery mildew
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Aegilops markgrafii contains resistance genes to powdery mildew, leaf rust and stripe rust, and also has high crude protein and lysine contents, which can be useful for wheat improvement. These important traits are localized on different chromosomes. Disomic Triticum aestivum-Ae. markgrafii addition lines and euploid introgression lines showing leaf-rust and powdery mildew resistance were screened with RAPDs to detect chromosome-specific markers which can accelerate the breeding process. RAPD markers for all six available disomic addition lines were obtained. The additional chromosomes B, C, D, E, F and G were identified by three, three, three, two, one and seven primers, respectively. All three chromosome-B-specific RAPD markers demonstrated the presence of alien chromatin in the leaf-rust-resistant 42-chromosome introgression lines as well as in the segregating progeny. The three chromosome-C-identifying primers also demonstrated the presence of that chromosome in powdery mildew-resistant euploid introgression lines. The substitution lines (5A)5C and (5D)5C with different genetic backgrounds for both parents, in comparison to the lines mentioned above, showed the chromosome C-specific band with only two of the three primers. The chromosome F-specific primer and a primer evident on all the Ae. markgrafii chromosomes analysed did not generate the expected fragments on the chromosome Fdel addition line, indicating that the markers are located on the deleted part of chromosome F.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050498
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  • 36
    Digitale Medien
    Digitale Medien
    The development of a nuclear male sterility system in wheat. Expression of the barnase gene under the control of tapetum specific promoters (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 125-131 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Wheat ; Triticum aestivum ; Transformation ; Nuclear male sterility ; DNA-integration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Nuclear male sterility within Triticum aestivum is considered as the ideal basis for the development of a hybridization system for wheat. We engineered nuclear male sterility in wheat by introducing the barnase gene under the control of tapetum-specific promoters derived from corn and rice. A biolistic-mediated transformation method, based on the use of the poly(ADP-ribose)polymerase inhibitor niacinamide, was set up which enriched for low-copy integrations (1–3 copies). Most of these copies were not linked and segregated in the next generation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050540
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  • 37
    Digitale Medien
    Digitale Medien
    A cereal cyst nematode (Heterodera avenae Woll.) resistance gene transferred from Aegilops triuncialis to hexaploid wheat (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 1135-1140 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Aegilops triuncialis ; Triticum aestivum ; Heterodera avenae ; Cereal cyst nematode ; Resistance gene
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The cereal cyst nematode (Heterodera avenae) is an important root parasite of common wheat. A high level of resistance was transferred to wheat from Aegilops triuncialis (TR lines) using the cross [(T. turgidum×Ae. triuncialis)×T. aestivum]. Low fertility (3–5 viable kernels per plant) was observed during the process but the surviving hybrid plants were highly vigorous. To obtain stable resistant lines further crosses to T. aestivum were performed. The resistance in TR lines seems to be transferred from the C genome of Ae. triuncialis (genomes CCUU). Ae. triuncialis was highly resistant to the two Spanish populations of H. avenae tested, as well as to four French races and two Swedish populations. The histological analysis showed a hypersensitive reaction in the roots of a resistant TR line inoculated with the Ha71 pathotype of H. avenae, whereas well-formed syncytia were observed in the roots of the susceptible control. Resistance to the H. avenae Ha71 pathotype seemed to be inherited as determined by a single dominant factor in the crosses between resistant TR lines and susceptible cultivars.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050849
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  • 38
    Digitale Medien
    Digitale Medien
    Measuring the effect of an abiotic stress on competition (1994)
    Springer
    Oecologia 100 (1994), S. 221-228 
    Details
    ISSN: 1432-1939
    Schlagwort(e): Triticum aestivum ; Competition ; Abiotic stress ; Multiplicative interactions ; Nickel toxicity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Using recently developed solution culture techniques, the effect of a non-resource abiotic stress, nickel toxicity, was tested on intraspecific nutrient competition among wheat. The choice of an appropriate statistical model was of paramount importance in interpreting these effects. We argue that a multiplicative model is more appropriate for experiments on interactions of competition and abiotic stress. By such an analysis, nickel had no relative effect on the ability of competition to reduce plant size in two experiments, and caused a small reduction in competition in another. These results are contrary to other reports of the effect of a non-resource abiotic stress on competition and appear to be due to an increased demand for nutrients in the presence of toxic levels of nickel. The effects of an abiotic stress on competition may thus be specitic to the stress and not generalized across all abiotic stresses.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00316948
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  • 39
    Digitale Medien
    Digitale Medien
    Plant competition and disease in genetically diverse wheat populations (1992)
    Springer
    Oecologia 91 (1992), S. 82-92 
    Details
    ISSN: 1432-1939
    Schlagwort(e): Plant disease ; Genetic diversity ; Frequency-dependence ; Triticum aestivum ; Puccinia striiformis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The direct and indirect effects of plant genetic diversity on epidemics and the influence of disease on plant competition were investigated using the wheat (Triticum aestivum)/stripe rust (Puccinia striiformis) system. Replacement series consisting of a susceptible and a resistant wheat genotype or two wheat genotypes susceptible to different races of stripe rust were grown in the presence and absence of the pathogen. Stripe rust severity, number of seed heads, seed yield, and seed weight were determined separately for each wheat genotype in the mixtures and the pure stands. The frequency of susceptible genotypes in a mixture explained up to 67% of the variation in disease severity. However, competitive interactions among plant genotypes sometimes appeared to alter susceptibility and obscured the relationship. In pure stands of single genotypes, disease severity explained between 52 and 58% of the variation in seed yield. In mixtures, coefficients of determination were only 10 and 31%, suggesting a strong influence of plant-plant interactions on seed yield. These results suggest that host-parasite coevolutionary models need to account for the strong effect that specific plant genotype combinations may have on disease severity and plant reproduction.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00317245
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  • 40
    Digitale Medien
    Digitale Medien
    Plant competition for light analyzed with a multispecies canopy model (1990)
    Springer
    Oecologia 82 (1990), S. 304-310 
    Details
    ISSN: 1432-1939
    Schlagwort(e): Multispecies canopy model ; Canopy photosynthesis model ; Triticum aestivum ; Avena fatua ; Ultraviolet-B radiation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Competition for light among species in a mixed canopy can be assessed quantitatively by a simulation model which evaluates the importance of different morphological and photosynthetic characteristics of each species. A model was developed that simulates how the foliage of all species attenuate radiation in the canopy and how much radiation is received by foliage of each species. The model can account for different kinds of foliage (leaf blades, stems, etc.) for each species. The photosynthesis and transpiration for sunlit and shaded foliage of each species is also computed for different layers in the canopy. The model is an extension of previously described single-species canopy photosynthesis simulation models. Model predictions of the fraction of foliage sunlit and interception of light by sunlit and shaded foliage for monoculture and mixed canopies of wheat (Triticum aestivum) and wild oat (Avena fatua) in the field compared very well with measured values. The model was used to calculate light interception and canopy photosynthesis for both species of wheat/wild oat mixtures grown under normal solar and enhanced ultraviolet-B (290–320 nm) radiation (UV-B) in a glasshouse experiment with no root competition. In these experiments, measurements showed that the mixtures receiving enhanced UV-B radiation had a greater proportion of the total foliage area composed of wheat compared to mixtures in the control treatments. The difference in species foliage area and its position in the canopy resulted in a calculated increase in the portion of total canopy radiation interception and photosynthesis by wheat. This, in turn, is consistent with greater canopy biomass of wheat reported in canopies irradiated with supplemental UV-B.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00317475
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  • 41
    Digitale Medien
    Digitale Medien
    Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 407-414 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Keywords AFLPs ; Bulked segregant analysis ; Marker-assisted selection ; Microsatellites ; Powdery mildew resistance ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Molecular markers were identified in common wheat for the Pm24 locus conferring resistance to different isolates of the powdery mildew pathogen, Erysiphe graminis DM f. sp. tritici (Em. Marchal). Bulked segregant analysis was used to identify amplified fragment length polymorphism (AFLP) markers and microsatellite markers linked to the gene Pm24 in an F2 progeny from the cross Chinese Spring (susceptible)× Chiyacao (resistant). Two AFLP markers XACA/CTA-407 and XACA/CCG-420, and three microsatellite markers Xgwm106, Xgwm337 and Xgwm458, were mapped in coupling phase to the Pm24 locus. The AFLP marker locus XACA/CTA-407 co-segregated with the Pm24 gene, and XACA/CCG-420 mapped 4.5 cM from this gene. Another AFLP marker locus XAAT/CCA-346 co- segregated in repulsion phase with the Pm24 locus. Pm24 was mapped close to the centromere on the short arm of chromosome 1D, contrary to the previously reported location on chromosome 6D. Pm24 segregated independently of gene Pm22, also located on chromosome 1D. An allele of microsatellite locus Xgwm337 located 2.4±1.2 cM from Pm24 was shown to be diagnostic and therefore potentially useful for pyramiding two or more genes for powdery mildew resistance in a single genotype.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051497
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  • 42
    Digitale Medien
    Digitale Medien
    Extensive mitochondrial DNA variation in somatic tissue cultures initiated from wheat immature embryos (1987)
    Springer
    Current genetics 12 (1987), S. 369-376 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Triticum aestivum ; Tissue culture ; Mitochondrial DNA ; Genomic variability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Wheat mitochondria) DNA has been isolated from callus cultures initiated from both immature embryos and the corresponding parental cultivar. A Sall restriction pattern study has shown that the organization of callus culture mitochondria) DNA underwent extensive change, characterized by either the disappearance or the decrease in the relative stoichiometry of several restriction bands. Hybridization of labelled mitochondrial fragments obtained from a recombinant cosmid library to Southern blots of callus and parental line restricted mitochondria) DNAs has shown that a fraction of the mitochondria) genome was lost in callus cultures. Data from a Sall + HindIII restriction map of a defined part of the wheat mitochondria) genome concerned with some of these variations strongly suggest that the observed variations correspond to the disappearance of at least one mitochondria) DNA subgenomic molecule in callus cultures.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00405759
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  • 43
    Digitale Medien
    Digitale Medien
    Nucleotide sequence and determination of the extremities of the 26S ribosomal RNA gene in wheat mitochondria: evidence for sequence rearrangements in the ribosomal genes of higher plants (1988)
    Springer
    Current genetics 13 (1988), S. 75-82 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Triticum aestivum ; Plant mitochondrial DNA ; Ribosomal genes ; Sequence rearrangements
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The nucleotide sequence of the wheat mitochondrial 26S ribosomal RNA gene and flanking regions was determined and compared with mitochondrial 26S rRNA genes from maize and Oenothera. All three genes exhibit a high degree of homology except within two variable regions. When the plant mitochondrial 26S rRNA genes are compared with Escherichia coli 23S rRNA and chloroplast 23S and 4.5S rRNA genes, a third variable region is apparent close to the 3′ end of the gene. The 5′ and 3′ ends of the wheat mitochondrial gene were determined by S1 nuclease mapping. Computer analysis of the wheat mitochondrial gene revealed several small sequences present either in the 5′ region of the 26S rRNA gene or in the 18S rRNA gene.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00365760
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  • 44
    Digitale Medien
    Digitale Medien
    Evidence for a direct relationship between mitochondrial genome organization and regeneration ability in hexaploid wheat somatic tissue cultures (1988)
    Springer
    Current genetics 14 (1988), S. 387-394 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Triticum aestivum ; Genomic variability ; Mitochondrial DNA ; Somatic tissue culture ; Regeneration ability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Embryogenic and non-embryogenic long-term callus cultures of hexaploid wheat exhibit differences in the organization of their mitochondrial genome. Embryogenic and non-embryogenic fractions of callus cultures initiated from immature embryos of the wheat cultivar “Chinese Spring” have been isolated and subsequently subcultured. DNA-DNA hybridization experiments using labelled cloned wheat mitochondrial DNA fragments have shown that the mitochondrial DNA organization of embryogenic subcultures derived from embryogenic parts of “Chinese Spring” calli is closely related to that of the initial “Chinese Spring” calli, while non-embryogenic subcultures derived from non-embryogenic fragments of “Chinese Spring” calli exhibit a mitochondrial DNA organization similar to that found in non-embryogenic calli derived from cultivar “Aquila”. In addition, somatic tissue cultures initiated from three other non-embryogenic wheat cultivars (“Talent”, “Thésée” and “Capitole”) display mitochondrial DNA arrangements similar to those found in cultivar “Aquila”. These results strongly suggest that, in wheat callus cultures, a particular mitochondrial genome organization is correlated with the ability of cultured cells to regenerate whole plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00419997
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  • 45
    Digitale Medien
    Digitale Medien
    Mitochondrial DNA variability detected in a single wheat regenerant involves a rare recombination event across a short repeat (1994)
    Springer
    Current genetics 25 (1994), S. 456-464 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Reciprocal recombination ; Mitochondrial genome ; Chondriome variability ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The mitochondrial genome of the selfed progeny of a plant regenerated from long-term somatic tissue culture displays specific structural rearrangements characterized by the appearance of novel restriction fragments. A mitochondrial DNA library was constructed from this selfed progeny in the SalI site of cosmid pHC79 and the novel fragments were subsequently studied. They were shown to arise from reciprocal recombination events involving DNA sequences present in the parental plant. The regions of recombination were sequenced and the nucleotide sequences were aligned with those of the presumptive parental fragments. We characterized an imperfect short repeated DNA sequence, 242 bp long, within which a 7-bb DNA repeat could act as a region of recombination. The use of PCR technology allowed us to show that these fragments were present in both parental plants and tissue cultures as low-abundance sequence arrangements.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00351786
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  • 46
    Digitale Medien
    Digitale Medien
    Physical organization of the 18S and 5S ribosomal RNA genes in the mitochondrial genome of rye (Secale cereale L.) (1990)
    Springer
    Current genetics 17 (1990), S. 339-346 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Secale cereale ; Triticum aestivum ; Ribosomal RNA genes ; Mitochondrial DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The mitochondrial 18S and 5S ribosomal RNA (rRNA) genes of rye, plus a total of about 90 kilobase pairs of flanking DNA, have been cloned and maps of restriction enzyme cleavage sites have been constructed. Like their homologs from hexaploid wheat, the rye genes are closely linked and are part of a three-copy family of recombining repeats (the “18S/5S repeat”). The rye repeat probably also contains a mitochondrial tRNAfMet gene, which the wheat repeat is known to carry. However, despite the overall organizational similarity between the wheat and rye 18S/5S repeats in the immediate vicinity of their coding regions, extensive rearrangement of flanking sequences has taken place during evolutionary divergence of the two species. Our data provide additional support for an emerging picture of plant mitochondrial genomes as evolving much more rapidly in structure than in sequence.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00314882
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  • 47
    Digitale Medien
    Digitale Medien
    An internal part of the chloroplast atpA gene sequence is present in the mitochondrial genome of Triticum aestivum: molecular organisation and evolutionary aspects (1990)
    Springer
    Current genetics 17 (1990), S. 523-528 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Triticum aestivum ; Mitochondrial DNA ; Chloroplast DNA ; ATPase alpha subunit
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary An internal part of the chloroplast atpA gene has been identified in the mitochondrial DNA of Triticum aestivum. It is located near the 18S-5S ribosomal genes and partially contained within a repeated sequence. Comparison of the transferred sequence with the original ct sequence reveals several nucleotide changes and shows that neither 5′ nor 3′ ends are present in the mt genome. No transcript of this region could be detected by Northern analysis. This sequence is present in mitochondrial genomes of other tetraploid and diploid species of Triticum, also in the vicinity of the 18S-5S ribosomal genes, suggesting a unique transfer event. The date of this event is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00313082
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  • 48
    Digitale Medien
    Digitale Medien
    Differential expression of the psbB and psbH genes encoding the 47 kDa chlorophyll a-protein and the 10 kDa phosphoprotein of photosystem II during chloroplast development in wheat (1991)
    Springer
    Current genetics 19 (1991), S. 199-206 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Light regulation ; psbN ; Triticum aestivum ; Etioplast
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The nucleotide sequence of a region of wheat chloroplast DNA containing the psbB gene for the 47 kDa chlorophyll a-binding protein of photosystem II has been determined. The gene encodes a polypeptide of 508 amino acid residues which is predicted to contain six hydrophobic membrane-spanning regions. The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. A small open reading frame of 38 codons is located between psbB and psbH, and on the opposite strand the psbN gene, encoding a photosystem II polypeptide of 43 amino acid residues, is located between orf38 and psbH. S1 nuclease mapping indicated that the 5′ ends of transcripts were located 371 and 183 bp upstream of the psbB translation initiation codon. Predominant transcripts of 2.1 kb and 1.8 kb for psbB and 0.4 kb for psbH were present in RNA isolated from etiolated and greening wheat seedlings. Immunodecoration of Western blots indicated that the 47 kDa polypeptide was absent, or present in very low amounts, in dark-grown tissue and accumulated on greening, whereas the 10 kDa polypeptide was present in similar amounts in both dark-grown and greening seedlings. The 10 kDa polypeptide was phosphorylated in vitro by incubating wheat etioplast membranes with [γ3 2P] ATP.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00336487
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  • 49
    Digitale Medien
    Digitale Medien
    Sequence analysis of wheat mitochondrial transcripts capped in vitro: definitive identification of transcription initiation sites (1991)
    Springer
    Current genetics 20 (1991), S. 245-251 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Mitochondrion ; Transcription ; Guanylyltransferase ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary To identify transcription initiation sites in wheat mitochondria, the nascent 5′-ends of transcripts were specifically labeled by incubation of wheat mitochondrial RNA with [α-32P]GTP in the presence of the enzyme guanylyltransferase. After separation of the resulting capped transcripts by electrophoresis in polyacrylamide gels, individual RNAs were recovered and directly sequenced. Four RNA sequences obtained in this way were localized upstream of the protein-coding genes atpA, coxII, coxIII and orf25. Comparison of mRNA and gene sequences allowed precise positioning of transcription initiation sites for these four genes. Sequence similarities immediately upstream of these sites define a conserved motif that we suggest as a candidate regulatory element in wheat mtDNA. The relationship between this motif and putative mitochondrial promoters in other plant species is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00326239
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  • 50
    Digitale Medien
    Digitale Medien
    Nuclear genes control changes in the organization of the mitochondrial genome in tissue cultures derived from immature embryos of wheat (1992)
    Springer
    Current genetics 21 (1992), S. 515-520 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Nuclear-cytoplasmic interactions ; Mitochondrial genome ; Chondriome variability ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Although the mitochondrial genomes of the Chinese Spring and Aquila varieties of wheat are normaly similar in organization, this is not so in tissue cultures initiated from their immature embryos where the mitochondrial genomes of both are rearranged and in different, characteristic, ways. However, the mitochondrial genomes of tissue cultures of reciprocal F1 crosses between these varieties were almost identical to one another, showing that nuclear genes control the rearrangement processes. These rearrangements are either due to the appearance of new structures or else result from changes in the relative amounts of subgenomic components. The severe reduction in the amount of certain molecular configurations in tissue cultures from reciprocal crosses is probably due to the presence of dominant information in the Aquila nuclear genome. Data obtained from tissue cultures initiated from F2 embryos of the cross Aquila x Chinese Spring suggest that at least two complementary genes are involved in this control. In contrast, the presence of new molecular arrangements appears to be under the control of a dominant allelic form of a Chinese Spring gene or genes. Thus, this study demonstrates that at least two sets of nuclear genes control the reorganization of the mitochondrial genome which occurs when tissue cultures are initiated from the immature embryos of wheat.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00351662
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  • 51
    Digitale Medien
    Digitale Medien
    Comparative analysis of a recombining-repeat-sequence family in the mitochondrial genomes of wheat (Triticum aestivum L.) and rye (Secale cereale L.) (1993)
    Springer
    Current genetics 23 (1993), S. 255-264 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Triticum aestivum ; Secale cerele ; Ribosomal RNA genes ; Mitochondrial DNA ; Recombining-repeats ; Evolution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The mitochondrial genomes of wheat and rye each contain a three-member family of recombining repeat peat sequences (the “18S/5S repeat”) that encode genes for 18S and 5S rRNAs (rrn18 and rrn5) and tRNAfMet (trnfM). Here we present, for wheat and rye, the sequence and boundaries of the “common sequence unit” (CSU) that is shared between all three repeat copies in each species. The wheat CSU is 4,429 base-pairs long and contains (in addition to trnfM, rrn18 and rrn5) a putative promoter, three tRNA-like elements (“t-elements”), and part of a pseudogene (“ψatpA c”) that is homologous to chloroplast atpA, which encodes the α subunit of chloroplast F1 ATPase. The rye CSU is somewhat smaller (2,855 base pairs) but contains much the same genic and other sequence elements as its wheat counterpart, except that two of the three t-elements as well as ψatpA c are found in only one of the three downstream flanks of the 18S/5S repeat, outside the CSU boundaries. In interpreting the seuuence data in terms of the evolutionary history of the 18S/5S-repeat family of wheat and rye, we conclude that (1) the wheat-rye form of the 18S/5S repeat most likely originated between 3 and 14 million years ago, in a lineage that gave rise to wheat and rye but not to barley, oasts, rice or maize; (2) the close linkage (1-bp apart) between trnfM and rrn18 is similarly limited in its taxonomic distribution to the wheat/rye lineage; (3) the trnfM-rrn18 pair arose via a single mutation that inserted a sequence block containing trnfM immediately upstream of rrn18; and (4) the presence of a putative promoter upstream of rrn18 in all wheat and rye repeats is consistent with all three repeat copies being transcriptionally active. We discuss these conclusions in the light of the possible functional significance of recombining-repeats in plant mitochondrial genomes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00351504
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  • 52
    Digitale Medien
    Digitale Medien
    Effects of 15N-labelled ammonium nitrate and urea on soil nitrogen during growth of wheat (Triticum aestivum L.) under field conditions (1994)
    Springer
    Biology and fertility of soils 17 (1994), S. 232-236 
    Details
    ISSN: 1432-0789
    Schlagwort(e): 15N-labelled fertilizer ; Added N interaction ; Fertilizer N uptake ; Soil N uptake ; Wheat ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract We studied the effects of 15N-labelled ammonium nitrate and urea on the yield and uptake of labelled and unlabelled N by wheat (Triticum aestivum L., cv. Mexi-Pak-65) in a field experiment. The dry matter and N yields were significantly increased with fertilizer N application compared to those from unfertilized soil. The wheat crop used 33.6–51.5 and 30.5–40.9% of the N from ammonium nitrate and urea, respectively. Splitting the fertilizer N application had a significant effect on the uptake of fertilizer N by the wheat. The fertilizer N uptake showed that ammonium nitrate was a more available source of N for wheat than urea. The effective use of fertilizer N (ratio of fertilizer N in grain to fertilizer N in whole plant) was statistically similar for the two N fertilizers. The application of fertilizer N increased the uptake of unlabelled soil N by wheat, a result attributed to a positive added N interaction, which varied according to the fertilizer N split; six split applications gave the highest added N interaction compared to a single application or two split applications for both fertilizers. Ammonium nitrate gave 90.5, 33.5, and 48.5% more added N interaction than urea with one, two, and six split N applications. A values were not significantly correlated with the added N interaction (r=0.557). The observed added N interaction may have been the result of pool substitution, whereby added labelled fertilizer N replaced unlabelled soil N.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00336328
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  • 53
    Digitale Medien
    Digitale Medien
    Interaction of 15N-labelled ammonium nitrate, urea and ammonium sulphate with soil N during growth of wheat (Triticum aestivum L.) under field conditions (1995)
    Springer
    Biology and fertility of soils 20 (1995), S. 185-189 
    Details
    ISSN: 1432-0789
    Schlagwort(e): 15N-labelled fertilizer ; Added N interaction Fertilizer N uptake ; Soil N uptake ; Wheat ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The effects of 15N-labelled ammonium nitrate, urea and ammonium sulphate on yield and uptake of labelled and unlabelled N by wheat (Triticum aestivum L. cv. Mexi-Pak-65) were studied in a field experiment. The dry matter and N yields were significantly increased with fertilizer N application compared to those from unfertilized soil. The wheat crop used 64.0–74.8%, 61.5–64.7% and 61.7–63.4% of the N from ammonium nitrate, urea and ammonium sulphate, respectively. The fertilizer N uptake showed that ammonium nitrate was a more available source of N for wheat than urea and ammonium sulphate. The effective use of fertilizer N (ratio of fertilizer N in grain to fertilizer N in whole plant) was statistically similar for the three N fertilizers. The application of fertilizer N increased the uptake of unlabelled soil N by wheat, a result attributed to a positive added N interaction, which varied with the method of application of fertilizer N. Ammonium nitrate, urea and ammonium sulphate gave 59.3%, 42.8% and 26.3% more added N interaction, respectively, when applied by the broadcast/worked-in method than with band placement. A highly significant correlation between soil N and grain yield, dry matter and added N interaction showed that soil N was more important than fertilizer N in wheat production. A values were not significantly correlated with added N interaction (r=0.719). The observed added N interaction may have been the result of pool substitution, whereby added labelled fertilizer N stood proxy for unlabelled soil N.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00336556
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  • 54
    Digitale Medien
    Digitale Medien
    Effect of certain herbicides on growth and pathogenicity of take-all fungus on wheat (Triticum aestivum L.) (1987)
    Springer
    Biology and fertility of soils 5 (1987), S. 31-35 
    Details
    ISSN: 1432-0789
    Schlagwort(e): Gaeumannomyces graminis ; VegVetative growth ; Pathogenicity ; Herbicides ; Diquat+paraquat ; Glyphosate ; Dicamba ; Trifluralin ; Chlorsulfuron ; Chlorthal dimethyl ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The effects of Spray Seed (diquat + paraquat), Roundup (glyphosate), Banvel-D (dicamba), Treflan (trifluralin), Glean (chlorsulfuron) and Dacthal (chlorthal dimethyl) at concentrations of 0–500 ppm product on the vegetative growth, vigour and pathogenicity of Gaeumannomyces graminis var. tritici (Ggt) on wheat were examined. All herbicides with the exception of dicamba and chlorsulfuron inhibited fungal growth on potato dextrose agar (PDA) at concentrations 10–500-fold of rates recommended for use in the field. The vegetative growth of the pathogen growing out of straw colonized on PDA supplemented with 100 ppm diquat + paraquat or glyphosate was reduced by 47.4% and 42.4%, respectively. When portions of these colonies were subcultured onto unamended PDA, their growth and the pathogenicity of straw pieces colonized by these subcultures were found to be unaltered. Straw colonized by Ggt on agar amended with concentrations of diquat + paraquat or at all concentrations of glyphosate produced less root disease in wheat seedlings in comparison to those colonized on unamended agar. It is proposed that the reduced pathogenicity of inocula prepared on agar amended with these two herbicides is due to poor colonization by the pathogen of straw on these media, and that a similar effect on saprophytic colonization in the field could lead to a reduction in the field inocula of the pathogen.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00264343
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  • 55
    Digitale Medien
    Digitale Medien
    Influence of biopores on root growth, water uptake and grain yield of wheat (Triticum aestivum) based on predictions from a computer model (1988)
    Springer
    Biology and fertility of soils 6 (1988), S. 315-321 
    Details
    ISSN: 1432-0789
    Schlagwort(e): Biopores ; Root growth ; Transpiration ; Simulations ; Mechanical impedance ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The use of vertical biopores by wheat (Triticum aestivum) seminal roots for easy access to the subsoil and the consequences for plant water supply and yield has been investigated by computer simulation. Parameters included were: biopore density and diameter, depth of cultivation and strength of the subsoil — all under a wide range of seasonal weather conditions. The model predicts that biopores add significantly to root penetration at depth, even at a density of 0.1% v/v of small, vertical pores, while 1.5% to 2.0% v/v can ensure maximum root penetration. When the growing season is shorter a larger number of biopores is needed to ensure timely root penetration to depth. With shallow tillage, biopores occur closer to the soil surface, and their importance is increased. Deeper root penetration invariably gives greater water uptake and transpiration, but may have a negative effect on grain yield, especially under the driest climatic conditions. An increase in early water use may result in less soil water being available during the grain-filling period. The effect of biopores on plant transpiration varies from year to year, depending on the amount of rain and its distribution in time, and on the amount of soil water stored at time of sowing.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00261020
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  • 56
    Digitale Medien
    Digitale Medien
    The chromosomal location of peroxidase isozymes of the wheat kernel (1979)
    Springer
    Theoretical and applied genetics 55 (1979), S. 73-76 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Wheat, Peroxidases
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The analysis of the individual parts of the Triticum aestivum L. kernel yields a total of 11 peroxidase isozymes: m, n, a, c, d1, d, d2, e, f, g and h (in order from faster to slower migration). Isozymes a, c and d are found in the endosperm (Ed) and seed coats (C), while m, n, d1, d2, e, f, g and h are peculiar to the embryo and scutellum (E + S). The use of the nullitetrasomic and ditellosomic series of ‘Chinese Spring’ wheat allows peroxidase isozymes to be associated with specific chromosome arms. Isozymes a, c and d (Ed) are associated with chromosome arms 7DS, 4BL and 7AS; whereas isozymes m, d2, e and f are associated with chromosome arms 3DS, 3BL, 3DL and 3DL, respecitvely. Thus, the E + S isozymes are associated with homoeology group 3 and the Ed isozymes with homoeology groups 7 (a and d isozymes) or 4 (c isozymes).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00285193
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  • 57
    Digitale Medien
    Digitale Medien
    Combining ability analysis over F1-F5 generations in diallel crosses of bread wheat (1979)
    Springer
    Theoretical and applied genetics 55 (1979), S. 77-80 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Combining ability analysis ; Triticum aestivum ; Diallel crosses
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Combining ability studies for grain yield and its primary component traits in diallel crosses involving seven diverse wheat cultivars of bread wheat (Triticum aestivum L.) over generations F1-F5 are reported. The general and specific combining ability variances were significant in all generations for all the traits except specific combining ability variance for number of spikes per plant in the F5. The ratio of general to specific combining ability variances was significant for all the traits except grain yield in all the generations. This indicated an equal role of additive and non-additive gene effects in the inheritance of grain yield, and the predominance of the former for its component traits. The presence of significant specific combining ability variances in even the advanced generations may be the result of an additive x additive type of epistasis or evolutionary divergence among progenies in the same parental array. The relative breeding values of the parental varieties, as indicated by their general combining ability effects, did not vary much over the generations. The cheap and reliable procedure observed for making the choice of parents, selecting hybrids and predicting advanced generation (F5) bulk hybrid performance was the determination of breeding values of the parents on the relative performance of their F2 progeny bulks.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00285194
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  • 58
    Digitale Medien
    Digitale Medien
    Induction of haploid and diploid plants though in vitro anther culture of haploid wheat (n=3x=21) (1980)
    Springer
    Theoretical and applied genetics 57 (1980), S. 57-58 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Anther culture ; Haploid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Five haploid plants of wheat were used for anther culture. Embryos were formed and six plants were regenerated. Of these, two were haploid (n=3x=21) and two diploid (2n=6x=42). The two diploids derived from the anthers of the same haploid wheat plant gave seeds, but the fertility was reduced in one of them showing, abnormalities at meiosis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00276401
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  • 59
    Digitale Medien
    Digitale Medien
    Induction of haploid and diploid plants though in vitro anther culture of haploid wheat (n=3x=21) (1980)
    Springer
    Theoretical and applied genetics 57 (1980), S. 57-58 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Anther culture ; Haploid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Five haploid plants of wheat were used for anther culture. Embryos were formed and six plants were regenerated. Of these, two were haploid (n=3x=21) and two diploid (2n=6x=42). The two diploids derived from the anthers of the same haploid wheat plant gave seeds, but the fertility was reduced in one of them showing, abnormalities at meiosis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00745029
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  • 60
    Digitale Medien
    Digitale Medien
    Monosomic analysis of genes for resistance against stem rust races in bread wheat (1981)
    Springer
    Theoretical and applied genetics 59 (1981), S. 313-316 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Wheat ; Puccinia graminis ; Stem rust resistance ; Monosomic analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Using monosomic analysis genes governing resistance in one wheat variety, ‘E5883’, against stem rust races 15C and 122, were located on specific chromosomes. Against race 15C, a major dominant gene, Sr6, was located on chromosome 2D of ‘E5883’ with minor modifiers found on chromosomes 4A and 6D. Against race 122, the presence of a recessive hemizygous ineffective gene, Sr8, was identified, located on chromosome 6A of variety ‘E5883’.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00264984
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  • 61
    Digitale Medien
    Digitale Medien
    The chromosomal location of factors determining the presence of phenolic compounds in wheat (Triticum aestivum L.) (1982)
    Springer
    Theoretical and applied genetics 61 (1982), S. 125-128 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Wheat-phenolics-location
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Using thin-layer chromatography and nulli-tetrasomic and ditellosomic series of Triticum aestivum L. cv. ‘Chinese Spring’, it has been possible to relate the phenolic compounds found in adult plant leaves and 12 day-old seedling leaves with the chromosomes or chromosome arms 1 B, 2 BL, 3 BL, 5 A, 6 AL, 7 B and 7 DS.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00273879
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  • 62
    Digitale Medien
    Digitale Medien
    N-banding in Triticum aestivum following feulgen hydrolysis (1982)
    Springer
    Theoretical and applied genetics 61 (1982), S. 337-339 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; ‘Chinese Spring’ ; Chromosome banding ; Hydrolysis ; Feulgen
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Terminal and/or interstitial N-bands were produced on the seven B-genome chromosomes and chromosomes 4 and 7 of the A-genome of Triticum aestivum cv. ‘Chinese Spring’ by a modified BSG technique following a standard Feulgen preparation. The banding was accomplished by modifying the barium hydroxide treatment.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00272852
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  • 63
    Digitale Medien
    Digitale Medien
    The chromosomal location of malate dehydrogenase isozymes in hexaploid wheat (Triticum aestivum L.) (1983)
    Springer
    Theoretical and applied genetics 64 (1983), S. 255-258 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Wheat ; Malate dehydrogenase ; Chromosomal location
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The malate dehydrogenase (E.C. no 1.1.1. 37) of Triticum aestivum L. cv. Chinese Spring, shows two activity zones. The results obtained support the hypothesis that the malate dehydrogenase isozymes of zone II are dimers composed of the six possible combinations of subunits coded by triplicate genes located in the long arms of chromosomes of the homoeologous group 1.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00303774
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  • 64
    Digitale Medien
    Digitale Medien
    Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis (1983)
    Springer
    Theoretical and applied genetics 66 (1983), S. 1-7 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Aegilops species ; Alloplasmic lines ; 2D gel electrophoresis ; Cytoplasmic inheritance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In this first analysis the protein patterns obtained by two-dimensional gel electrophoresis of 8 day-old leaves from 18 alloplasmic wheat lines are compared. From 440 spots retained on the basis of their reproducibility, 36 proteins were observed to vary in different cytoplasms, allowing us to distinguish the T. aestivum cytoplasm from 5 Aegilops cytoplasms. Twenty-four of the 36 variable proteins could be structurally related to the large subunit of RuBPCase. Nuclear variation between 3 wheat varieties was observed for 14 proteins.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00281838
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  • 65
    Digitale Medien
    Digitale Medien
    New hybrids between Agropyron and wheat (1983)
    Springer
    Theoretical and applied genetics 66 (1983), S. 111-121 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Agropyron ; Intergeneric hybrids ; Embryo culture ; Chromosome pairing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Intergeneric hybrids of Triticum aestivum (2n=42,AABBDD) with Agropyron ciliare (2n= 28,SSYY), A. trachycaulum (2n=28,SSHH), A. yezoense (2n=28,SSYY) and A. scirpeum (2n=28) are reported for the first time. F1 hybrids of T. aestivum were also produced with A. intermedium (2n=42,E1E1E2E2Z1Z1) and A. junceum (2n=14,JuJu). All wheat-Agropyron hybrids were obtained by embryo rescue technique. Cultivars and reciprocal crosses differed for seed set, seed development and F1 plant production. The F1 hybrids were sterile. Attempts to obtain amphiploids were unsuccessful. However, backcross derivatives were obtained with wheat as the recurrent parent. The level of chromosome pairing in A. trachycaulum x wheat, A. yezoense x wheat and wheat x A. junceum hybrids provided no evidence of homologous or homoeologous pairing. Mean pairing frequencies in A. ciliare x wheat, wheat x A. scirpeum and wheat x A. intermedium hybrids indicated homoeologous or autosyndetic pairing. Ph gene was more effective in regulating homoeologous pairing in A. yezoense x wheat hybrids than in A. ciliare x wheat hybrid. Chromosome pairing data of BC1 derivatives indicated that either some of the wheat chromosomes were eliminated or Agropyron chromosomes caused reduced pairing of wheat homologues.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00265184
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  • 66
    Digitale Medien
    Digitale Medien
    Control of lectins in Triticum aestivum and Aegilops umbellulata by homoeologous group 1 chromosomes (1983)
    Springer
    Theoretical and applied genetics 67 (1983), S. 53-58 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Aegilops umbellulata ; Genetics ; Lectin ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Each of the three genomes in hexaploid wheat controls the expression of a specific lectin in the embryo. The chromosomes which control their synthesis were determined using nullisomic-tetrasomic and inter-varietal chromosome substitution lines of ‘Chinese Spring’. All three wheat lectins were shown to be controlled by the homoeologous group 1 chromosomes. Using ditelosomic lines of ‘Chinese Spring’ the lectin genes could be localized on the long arms of chromosomes 1A and 1D. Inter-specific addition and substitution lines of Aegilops umbellulata chromosomes to ‘Chinese Spring’ indicated that chromosome 1U, which is homoeologous to the group 1 chromosomes of wheat, controls lectin synthesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00303922
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  • 67
    Digitale Medien
    Digitale Medien
    Blocks of gliadin components in winter wheat detected by one-dimensional polyacrylamide gel electrophoresis (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 559-568 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Gliadins ; Electrophoresis ; Hybridological analysis ; Genetic nomenclature
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Inheritance of gliadin components in winter wheat has been studied by one-dimensional polyacrylamide gel electrophoresis. Single F2 grains from 36 intervarietal hybrid combinations have been analysed. The genetic analysis has revealed blocks, including 1–6 gliadin components, which are inherited as individual mendelian traits. About 80 variants of blocks have been detected. On the basis of the allelism test they are grouped into 6 series in accordance with the number of known gliadin-coding loci located on chromosomes of the homoeologous groups 1 and 6. Each series includes 8–18 blocks controlled by different alleles of one gliadin-coding locus. Blocks of components have been confirmed to be inherited codominantly in accordance to the gene dose in the triploid endosperm. The highest similarity between members of one series is observed in groups of blocks controlled by chromosomes ID and 6D. On the contrary, many blocks controlled by chromosomes 1A and 1B have no bands in common. The presented catalogue of blocks of components may be used to make up gliadin genetic formulae and to compare electrophoregrams obtained by different authors. Blocks of gliadin components are suitable genetic markers for use in revealing and studying heterogeneity of wheat varieties, in tracing their origin, in identifying recombinations, translocations and substitutions of the genetic material and in solving many other problems of the origin, evolution and selection of hexaploid wheat.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00264904
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  • 68
    Digitale Medien
    Digitale Medien
    Genetic analysis of gliadin components in winter wheat using two-dimensional polyacrylamide gel electrophoresis (1984)
    Springer
    Theoretical and applied genetics 69 (1984), S. 31-37 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Gliadins ; Two-dimensional electrophoresis ; Hybridological analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Blocks of gliadin components found both in a number of varieties and in single F2 grains of winter wheat intervarietal hybrids have been studied by two-dimensional electrophoresis combining electrophoresis in acidic aluminium-lactate buffer (pH3.1) and SDS-electrophoresis. Gliadin components (spots) have been shown to be inherited as linked groups (blocks), codominantly and in accordance with a gene dosage in triploid endosperm. Blocks include components differing in their electrophoretic mobility and molecular weight. Some allelic variants of blocks differ only in presence of few additional components or in the electrophoretic mobility of components with similar molecular weights; other variants may contain no similar components. Apparently, in the course of evolution, mutations in individual genes of gliadin-coding loci and processes changing the number of expressing genes and the sizes of their structural part occurred.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00262533
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  • 69
    Digitale Medien
    Digitale Medien
    Intrachromosomal mapping of the nucleolar organiser region relative to three marker loci on chromosome 1B of wheat (Triticum aestivum) (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 263-270 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Intrachromosomal mapping ; Nucleolar organiser region ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Restriction enzyme digestion of the ribosomal RNA genes of the nucleolar organisers of wheat has revealed fragment length polymorphisms for the nucleolar organiser on chromosome 1B and the nucleolar organiser on 6B. Variation between genotypes for these regions has also been demonstrated. This variation has been exploited to determine the recombination frequency between the physically defined nucleolar organiser on 1B (designatedNor1) and other markers; two loci,Glu-B1 andGli-B1 which code for endosperm storage proteins andRf3, a locus restoring fertility to male sterility conditioned byT. timopheevi cytoplasm.Gli-B1 andRf3 were located on the short-arm satellite but recombine with the nucleolar organiser giving a gene order ofNor1 — Rf3 — Gli-B1. Glu-B1 is located on the long arm of 1B but shows relatively little recombination withNor1, which is, in physical distance, distal on the short arm. This illustrates the discrepancy between map distance and physical distance on wheat chromosomes due to the distal localisation of chiasmata. The recombination betweenNor1 andRf3 indicates that, contrary to previous suggestions, fertility restoration is not a property of the nucleolar organiser but of a separate locus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00662440
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  • 70
    Digitale Medien
    Digitale Medien
    Comparative genetic analyses of metric traits using diallel and factorial mating designs in bread wheat (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 325-328 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Line × tester analysis ; Triticum aestivum ; Gene action ; Combining ability ; Mating designs
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary For studying the inheritance of metric traits, diallel cross and factorial mating designs are commonly used. Since factorial mating design is less restrictive in crossing plans, the genetic information drawn from it was compared with that from a diallel cross. The comparison was made using graphical, genetic components and combining ability analyses for grain yield, grain weight and spike length in a field experiment of bread wheat (Triticum aestivum L.). Analyses were made on a nine parent diallel cross and a 4 ♀ × 5 ♂ factorial mating design which was sampled from the diallel cross. In general, there was a high degree of agreement between the results obtained from factorial mating design and diallel cross analyses showing thereby that the former provides almost equivalent genetic information to the latter.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00662454
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  • 71
    Digitale Medien
    Digitale Medien
    Factors affecting the induction of pollen plants of intergeneric hybrids of Triticum aestivum X Triticum-Agropyron (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 294-299 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Anther culture ; Pollen callus (plantlet) ; Induction frequency ; Triticum aestivum ; Agropyron glaucum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Experimental results showed that the use of potato extract as a basic component of culture medium had a promoting effect on producing calli in anther culture of the intergeneric hybrids of Triticum aestivum × Triticum-Agropyron (intermediate type). The induction frequencies of pollen callus on the Potato-II medium containing potato extract as the main component was much higher than that found on N6 and W5 media. The induction frequencies of pollen callus and green plantlets in four intergeneric hybrid material inoculated at the late-uninucleate pollen stage were all higher than those inoculated at the mid-uninucleate stage. Appropriate increases in culture temperature significantly increased pollen callus induction frequencies of the intergeneric hybrids. The genotype and physiological state of anther donor plants also influenced pollen callus and green plantlet induction frequencies.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00304914
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  • 72
    Digitale Medien
    Digitale Medien
    Chromosomal localization of structural genes and regulators in wheat by 2D electrophoresis of ditelosomic lines (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 31-38 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; 2D electrophoresis ; Gene localization ; Homoeologous genes ; Genetic regulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Among the 782 spots observed in two-dimensional gel electrophoresis of denatured proteins from etiolated wheat shoots, 185 were found to be variable between the euploid and 26 ditelosomic lines of ‘Chinese Spring’. Thirty-five structural genes were located on 17 chromosome arms. Numerous intensity changes showing alterations in protein levels were observed and led to the following statements: 1) regulators are frequently found and can be assigned for a same polypeptide to various chromosome arms; 2) for most polypeptides homoeologous arms do not manifest similar effects; 3) nevertheless, when affecting the same polypeptide, homoeologous arms display in most cases identical regulatory effects; 4) gene dosage compensation is observed in only one out of four homoeoallelic situations.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00278250
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  • 73
    Digitale Medien
    Digitale Medien
    Nucleolus organizer competition in Triticum aestivum — Aegilops umbellulata chromosome addition lines (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 278-283 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Nucleolar competition ; Amphiplasty ; Ag-NORs ; Addition lines ; Triticum aestivum ; Aegilops umbellulata
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The nucleolar organizer activity of wheat (Triticum aestivum, AABBDD) and Aegilops umbellulata (UU) chromosomes have been analyzed in the complete set of the chromosome addition lines by using a highly reproducible silver-staining procedure. Chromosomes 1U and 5U produce the partial inactivation of wheat nucleolar organizer chromosomes 6B, 1B and 5D. The chromosomes D and G from Ae. umbellulata, which are not SAT-chromosomes, seem to specifically influence the activity of wheat NORs. The predominant status of the U genome with respect to nucleolar competition in the Triticeae is confirmed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00252067
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  • 74
    Digitale Medien
    Digitale Medien
    Production, morphology, and cytogenetic analysis of Elymus caninus (Agropyron caninum) x Triticum aestivum F1 hybrids and backcross-1 derivatives (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 750-756 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Agropyron caninum ; Elymus caninus ; Intergeneric hybrids ; Chromosome pairing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Intergeneric hybrids were produced between common wheat, Triticum aestivum (2n=6x=42, AABBDD) and wheatgrass, Etymus caninus (Agropyron caninum) (2n=4x=28, SSHH) — the first successful report of this cross. Reciprocal crosses and genotypes differed for percent seed set, seed development and F1 hybrid plant production. With E. caninus as the pollen parent, there was no hybrid seed set. In the reciprocal cross, seed set was 23.1–25.4% depending upon wheat genotype used. Hybrid plants were produced only by rescuing embryos 12–13 days post pollination with cv ‘Chinese Spring’ as the wheat parent. Kinetin in the medium facilitated embryo germination but inhibited root development and seedling growth. The hybrids were vigorous, self sterile, and intermediate between parents. These had expected chromosome number (2n=5x=35, ABDSH), very little chromosome pairing (0.51 II, 0.04 III) and some secondary associations. The hybrids were successfully backcrossed with wheat. Chromosome number in the BC1 derivatives varied 54–58 with 56 as the modal class. The BC1 derivatives showed unusually high number of rod bivalents or reduced pairing of wheat homologues. These were sterile and BC2 seed was produced using wheat pollen.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00263274
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  • 75
    Digitale Medien
    Digitale Medien
    Heritable somaclonal variation in gliadin proteins of wheat plants derived from immature embryo callus culture (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 784-790 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Tissue culture ; Electrophoresis ; Storage proteins ; Mutation ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Fertile r0 plants of the winter wheat line ND7532 (Triticum aestivum L.) were regenerated from callus tissue after 60–190 days in culture. Seeds produced from these self-pollinated plants were planted in the field. Of the 5586 R1 plants, 32 differed for one or more agronomic traits from plants not passed through tissue culture process. Gliadin electrophoregrams were prepared from bulk samples of R2 seed from these 32 plants. Four of the 32 produced gliadin patterns different from controls, so 12 seeds of each of these four lines were examined individually. Three of the four mutant lines were fixed for the presence of a mutant protein of 50 relative mobility units (RMU) and the corresponding loss of a parental protein of 26 RMU. The remaining line segregated for the presence/absence of band 50 and the corresponding loss/retention of band 26. The mutant protein of 50 RMU was never seen in control plants. This indicated that either band 50 was coded for by a mutant gene allelic to the gene that coded for band 26 or that bands 26 and 50 were coded for by two different structural alleles under the control of a common regulatory locus. Each of the 12 seeds from the four mutant lines contained a prominent protein band at 30 (RMU), which was only observed as a faint band in one control seed. The types of variation in gliadin patterns observed in somaclones of ND7532 were similar to those reported for the line ‘Yaqui 50E’, except that, gliadin changes occurred less frequently in ND7532.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00276418
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  • 76
    Digitale Medien
    Digitale Medien
    Meiotic pairing and alpha-amylase phenotype in a 5B/5Rm Triticum aestivum — Secale montanum translocation line in common wheat (1986)
    Springer
    Theoretical and applied genetics 73 (1986), S. 122-128 
    Details
    ISSN: 1432-2242
    Schlagwort(e): α-amylase ; Triticum aestivum ; Secale montanum ; C-banding ; Meiotic pairing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A 5BS/5RmS translocation chromosome spontaneously recovered from a ‘Chinese Spring’ — Secale montanum wheat-rye telocentric 5RmS addition line has been identified and cytologically studied using C-banding in somatic and meiotic cells. Analysis of the translocated chromosome showed that a terminal segment of the short arm of 5B had been replaced by a short terminal region of chromosome arm 5RmS. The translocation led to the deletion of the genetic system promoting pairing located in 5BS, which is slightly compensated for when doses of 5RmS are increased, indicating homoeology to wheat chromosome 5BS. The α-amylase phenotype in 5B/5Rm translocated material was studied and found to be identical to that of ditelocentric line 5BL of ‘Chinese Spring’. An effect on the α-amylase activity was detected as a result of the removal of the terminal region of 5BS, perhaps as a consequence of variation in dormancy period duration.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00273728
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  • 77
    Digitale Medien
    Digitale Medien
    Regulatory effects of homoeologous chromosome arms on wheat proteins at two developmental stages (1986)
    Springer
    Theoretical and applied genetics 73 (1986), S. 246-251 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; 2D electrophoresis ; Gene localization ; Gene regulation ; Homoeology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Two-dimensional gel electrophoresis was conducted on denatured proteins of the 10-day-old first leaf (1F stage) of 18 homoeologous ditelosomic (DT) lines of wheat cultivar ‘Chinese Spring’. The observations, compared to the euploid control and relative to previous data found on 7-day-old etiolated seedlings (G7 stage) of the same lines lead to the following statements: 1) the structural genes of 24 spots can be assigned to 12 chromosome arms; 2) regulatory effects are completely different between the 1F and the G7 stages which may indicate that the regulation of protein amounts is often stage-specific; 3) no case of complete gene dosage compensation is observed among 4 groups of hypothesized homoeoallelic products; 4) homoeologous DT lines do not manifest similar effects which suggest the absence of homoeology for the detected regulatory effects.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00289281
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  • 78
    Digitale Medien
    Digitale Medien
    A somaclonal variant of wheat with additional β-amylase isozymes (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 459-464 
    Details
    ISSN: 1432-2242
    Schlagwort(e): β-amylase ; Isozymes ; Triticum aestivum ; Somaclonal variation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The progeny of 149 plants regenerated from tissue culture of immature wheat (Triticum aestivum) embryos were screened for variation in their grain β-amylase isozyme pattern. One regenerant was found which was heterozygous for a variant pattern characterized by the presence of at least five new isozyme bands, as well as an increased intensity in existing bands in two more positions. The F2 of a homozygous variant crossed back to the parent segregated in an approximate 3∶1 ratio but resolution of the gels was not sufficient to distinguish whether this represents a dominant or co-dominant single mutant gene. No chromosome abnormalities were evident in mitosis or meiosis of the homozygous variant or in the F1 of the variant crossed back to the parent. No recombination has been seen between the variant bands and production of multiple bands from a single locus is consistent with the nature of the known β-amylase loci. However, the variant bands were not evident in a survey of 111 diverse genotypes, nor were they present in developing grain of the parent cultivar. Therefore, this variant could represent a rare mutation leading to expression of a currently unexpressed locus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00262516
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  • 79
    Digitale Medien
    Digitale Medien
    A new selection criterion for yield in wheat (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 275-279 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Wheat ; Biomass ; Harvest index ; Selection criterion ; Tiller mortality ; Modified selection procedure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The mortality of young tillers to an extent of 36.5% under optimum cultural conditions was recorded in a field experiment. Attention is drawn to the necessity of minimising this loss and to diverting it towards productive tillers by applying selection pressure. Future yield advances may be achieved by selecting genotypes which tiller moderately in the vegetative phase, most of which survive to produce grains. The character association of each variety under consideration was studied for the suitability towards this objective. A modified selection procedure is suggested which may be advantageously applied to achieve this objective. The proposed new methodology may also be effectively applied to such other cereal crops as barley, triticale and oats.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00289980
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  • 80
    Digitale Medien
    Digitale Medien
    Genetics of resistance to Puccinia graminis tritici in ‘Chris’ and ‘W3746’ wheats (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 846-855 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Puccinia graminis tritici ; Triticum aestivum ; Monosomic analysis ; Rust resistance ; Gene identification
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary ‘Chris’ wheat possessed genes Sr5, Sr7a, Sr8a, Sr9g and Sr12. ‘W3746’, derived from the cross ‘Chris’/‘Baart’, possessed Sr7a and Sr12. The response conferred by Sr7a was influenced by the genetic background. Although Sr7a or Sr12 alone conferred no observable resistance upon adult plants, the adult resistances of ‘Chris’ and ‘W3746’ to predominant pathotypes appeared to be associated with the interaction of Sr7a and Sr12, or genes at closely linked loci.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00289389
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  • 81
    Digitale Medien
    Digitale Medien
    Chromosomal location of a K/Na discrimination character in the D genome of wheat (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 584-588 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; T. turgidum ; Aegilops squarrosa ; Cation uptake ; D genome
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary K/Na ratios have been determined in the leaves of salt-treated plants of 14 disomic substitution lines in which each of the D-genome chromosomes replaces the homoeologous A- or B-genome chromosome in the tetraploid wheat variety Langdon (AABB genome). Aneuploid lines of hexaploid bread wheat (cv Chinese Spring) having a reduced or an enhanced complement of chromosome 4D have also been examined. These investigations show that the gene(s) determining K/Na ratios in the leaves of wheat plants grown in the presence of salt is located on the long arm of chromosome 4D.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00288856
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  • 82
    Digitale Medien
    Digitale Medien
    Identification of callus types for long-term maintenance and regeneration from commercial cultivars of wheat (Triticum aestivum L.) (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 609-617 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Cereals ; Gramineae ; Somatic embryogenesis ; Triticum aestivum ; Wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Immature embryos, inflorescences, and anthers of eight commercial cultivars of Triticum aestivum (wheat) formed embryogenic callus on a variety of media. Immature embryos (1.0–1.5 mm long) were found to be most suitable for embryogenic callus formation while anthers responded poorly; inflorescences gave intermediate values. Immature embryos of various cultivars showed significant differences in callus formation in response to 11 of the 12 media tested. No significant differences were observed when the embryos were cultred under similar conditions on MS medium with twice the concentration of inorganic salts, supplemented with 2,4-D, casein hydrolysate and glutamine. Furthermore, with inflorescences also no significant differences were observed. Explants on callus formation media formed two types of embryogenic calli: an off-white, compact, and nodular callus and a white compact callus. Upon successive subcultures (approximately 5 months), the nodular embryogenic callus became more prominent and was identified as ‘aged callus’. The aged callus upon further subculture, formed an off-white, soft, and friable embryogenic callus. Both the aged and friable calli maintained their embryogenic capacity over many subculture passages (to date up to 19 months). All embryogenic calli (1 month old) from the different callus-forming media, irrespective of expiant source, formed only green shoots on regeneration media that developed to maturity in the greenhouse. There were no significant differences in the response of calli derived from embryos and inflorescences cultured on the different initiation media. Also, the shoot-forming capacity of the cultivars was not significantly different. Anther-derived calli formed the least shoots. Aged and friable calli on regeneration media also formed green shoots but at lower frequencies. Plants from long-term culture have also been grown to maturity in soil.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226873
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  • 83
    Digitale Medien
    Digitale Medien
    Mechanism of durable resistance: a new approach (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 229-232 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Puccinia recondita ; Adult plant resistance ; Resistance genes ; Durable resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Wheat genotypes, including backcross derivatives of ‘Thatcher’ carrying Lr10 and Lr23, substitution lines for Lr10 and Lr23 in Chinese Spring background and Chinese Spring and Thatcher were analysed against 21 pathotypes of leaf rust in seedling tests. Adult plant responses in all these stocks were observed in the field nurseries under exposure to the inoculum of the Indian virulent races of leaf rust. The seedling data demonstrated that both the substitution lines and the backcross derivatives for each gene carry identical pattern of infection for resistance. The high level of adult plant resistance in the substitution lines, in contrast to the backcross derivatives in Thatcher, has been postulated to be due to the combination of resistance contributed by Lr10 and adult plant Chinese Spring resistance or to Lr23 and Chinese Spring adult plant resistance. It has been suggested that genes Lr10 and Lr23 added to the Chinese Spring background provide sources for durable resistance, since Chinese Spring has continued to provide a moderate level of adult plant resistance to leaf rust for a very long time.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00288804
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  • 84
    Digitale Medien
    Digitale Medien
    Effects of auxin and cytokinin on induction of sister chromatid exchanges in cultured cells of wheat (Triticum aestivum L.) (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 521-524 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; SCEs ; Auxin ; Cytokinin ; Tissue culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In order to know the mutagenic effects of synthetic auxins (NAA, 2,4-D, and 2,4,5-T) and a cytokinin (kinetin) in vitro, sister chromatid exchanges (SCEs) were analyzed in cultured cells of a hexaploid wheat (Triticum aestivum L.). In the MS medium supplemented with 2.0 mg/l 2,4-D, the mean number of SCEs per cell was 15.2, and per pg of DNA, 0.42. No significant effect was found in the treatments of NAA or 2,4-D at concentrations of 0.5–10.0 mg/l, whereas more than 2.0 mg/l of 2,4,5-T induced dramatic increases of SCEs. Kinetin itself had no significant effect on SCE induction, but there was a tendency that SCEs induced by 2,4,5-T were suppressed by kinetin.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00290836
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  • 85
    Digitale Medien
    Digitale Medien
    Monosomic analysis of tissue culture response in wheat (Triticum aestivum L.) (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 625-632 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Tissue culture ; Callus ; Monosomic analysis ; Regeneration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The ability of immature embryos of wheat (Triticum aestivum L.) to respond in cell culture was examined in crosses between the ‘Wichita’ monosomic series and a highly regenerable line, ‘ND7532’. Segregation in disomic controls and 13 monosomic families showed a good fit to a monogenic ratio indicating a qualitative mode of inheritance. Segregation in the cross involving monosomic 2D showed a high frequency of regeneration (93.6%) and high callus growth rate (1.87 g/90 days) indicating that 2D is a critical chromosome. Modifying genes may be located on other chromosomes. Substitution of chromosomes from a low regenerable cultivar ‘Vona’ further indicated that the group 2 chromosomes, in particular chromosome 2D, possess genetic factors promoting callus growth and regeneration.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00262556
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  • 86
    Digitale Medien
    Digitale Medien
    Chromosomal location of genes controlling heat shock proteins in hexaploid wheat (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 873-878 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Heat shock proteins ; 2D electrophoresis ; Triticum aestivum ; Gene location ; Gene regulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The low molecular weight heat shock protein (HSP) profiles of the hexaploid wheat cultivar “Chinese Spring” and its ditelosomic series were characterized by isoelectric focusing polyacrylamide gel electrophoresis of denatured in vivo radiolabeled proteins. Comparisons of the ditelosomics (DTs) to the euploid “Chinese Spring” enabled the assignment of genes controlling 9 of the 13 targeted HSPs to seven chromosome arms. There did not appear to be a genome-specific action in the regulation of expression of these HSPs. There did appear to be a higher frequency of controlling genes within homoeologous DT lines 3, 4 and 7. Significant variation in protein quantity was evident among the DT lines for some HSPs, while other HSPs were remarkably stable in their expression across all DTs examined. The results are useful in identifying specific DT lines for the investigation of HSP functions in hexaploid wheat.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00266674
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  • 87
    Digitale Medien
    Digitale Medien
    In vitro microspore reaction of different German wheat cultivars (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 77-80 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Anther culture ; 1B/1R translocation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The in vitro microspore androgenesis reaction of 25 commercial German spring (including 4 Triticum durum) and 50 winter wheat cultivars was investigated. Tremendous genotypical differences were found in microspore response. The best-responding winter wheat cultivai, “Florida”, is characterized by the presence of a 1B/1R wheat-rye translocation chromosome. The significance of this finding and other genetic systems for future use of haploids in plant breeding is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223790
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  • 88
    Digitale Medien
    Digitale Medien
    Dosage effects of chromosomes of homoeologous groups 1 and 6 upon bread-making quality in hexaploid wheat (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 281-287 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Glutenin ; Gliadin ; Aneuploid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The endosperm storage proteins, glutenin and gliadin, are major determinants of bread-making quality in hexaploid wheat. Genes encoding them are located on chromosomes of homoeologous groups 1 and 6. Aneuploid lines of these groups in spring wheat cultivar ‘Chinese Spring’ have been used to investigate the effect of varying the dosage of chromosomes and chromosome arms upon bread-making quality, where quality has been assessed using the SDS-sedimentation test. Differences between the group 1 chromosomes for quality were greater than those between the group 6 chromosomes. The chromosomes were ranked within homoeologous groups for their effect on quality as follows (〉=better quality): 1D〉1B〉1A and 6A〉6B=6D. The relationship of chromosome dosage with quality was principally linear for four of the chromosomes, but not for 6B and 6D. Increases in the dosage of 1B, 6A and, especially, 1D, were associated with significant improvements in quality, whereas increases in the dosage of 1A were associated with reductions in quality. The effects of 1A and 1D were such that the best genotype for quality was nullisomic 1A-tetrasomic 1D. For group 1, effects of the long arm appeared in general to be more important than effects of the short arm. For group 6, effects were found associated with the long arms as well as with the short arms, a surprising result in view of the absence of genes encoding storage proteins on the long arms. Significant interactions were found between chromosomes and genetic backgrounds, and between individual chromosomes. Analysis of trials grown over two years demonstrated that, although additive environmental differences over years and genotype x years interaction were present, they were relatively small in magnitude compared with purely genetic differences.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224399
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  • 89
    Digitale Medien
    Digitale Medien
    A comparative evaluation of two methods of selecting locations used for testing spring wheat cultivars (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 301-304 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Genotype ; Environment interactions ; Cluster analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary One of the considerations of regional cultivar evaluation programs is to optimize the number of locations used for testing. Although optimization of numbers of locations using cluster analysis has been previously attempted, no objective comparison of methods has yet been made. A new clustering method that uses the pairwise contribution of locations to the cultivar x location mean square as the distance measure (LB) was compared to another method that employs diallel correlations as the distance measure (CL). Data from six spring wheat (Triticum aestivum L. em Thell) cultivars grown at 13 locations for five years were used in the initial cluster analysis. Another set of data, from a separate year, consisting of yields of the original 6 cultivars and a set of 12 independent cultivars was then used to check the validity of the original groupings and to compare the two methods. When the 6 original cultivars were considered, the LB technique was found to be superior to the CL. When the 12 independent cultivars were used, neither method was considered to be superior. Because of the lack of flexibility on the part of the LB method, neither technique could be deemed as fully adequate.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224275
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  • 90
    Digitale Medien
    Digitale Medien
    Isozyme and cytological markers of some Psathyrostachys juncea accessions (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 528-534 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Psathyrostachys juncea ; Triticum aestivum ; Isozyme markers ; Chromosome banding ; Intergeneric hybridization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Psathyrostachys juncea (synonymous to Elymus junceus; 2n=2x=14, NN) has unique biotic and abiotic attributes that could contribute towards wheat improvement. The effectiveness of such an intergeneric hybridization program depends greatly on being able to establish diagnostic markers of the alien chromosomes. Isoelectric focusing (IEF) analyses of six enzyme systems have identified five biochemical markers — malate dehydrogenase (MDH), esterase (EST), shikimate dehydrogenase (SKDH), phosphoglucomutase (PGM), and β-amylase (β-AMY) — to be of positive diagnostic value; glucosephosphate isomerase (GPI) banding profiles were of no definite value in the background of Triticum aestivum cvs ‘Chinese Spring’ and ‘Seri-82’, the potential recipients of Ps. juncea chromosomes. The Giemsa C-banding karyotype distinctively separates the Ps. Juncea chromosomes from each other and from those of T. aestivum with little banding site polymorphisms prevalent among its accessions analyzed, indicating the usefulness of C-bands as cytological markers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224148
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  • 91
    Digitale Medien
    Digitale Medien
    The modification of a common wheat-Thinopyrum distichum translocated chromosome with a locus homoeoallelic to Lr19 (1992)
    Springer
    Theoretical and applied genetics 85 (1992), S. 73-78 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Intergeneric gene transfer ; Allosyndetic recombination
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The ‘Chinese Spring’ ph1b and ph2b mutants, as well as the nulli 5B tetra 5D stock were utilized in an attempt to effect homoeologous chromatin exchange between the ‘Indis’ chromosome translocation [derived from Thinopyrum distichum (Thunb.) Löve] and chromosome arm 7DL of common wheat. A homoeoallele of Lr19 and linked genes for yellow flour-pigmentation were utilized as markers. Seven selections with recombinations involving the foreign, translocated segment were recovered. Four of these had white endosperms and were leaf-rust resistant. The remaining lines were leaf-rust resistant and had levels of endosperm pigmentation intermediate to those of ‘Indis’ and ‘Chinese Spring’. The recombined translocation segments coding for white endosperm are no longer associated with chromosome 7D. The original translocated segment may, therefore, not be fully homoeologous to 7DL. The recombinants with white endosperm also lack the stem-rust resitance gene Sr25, but retained the segregation distorter locus, Sd-1. However, it seems as though an enhancer locus (or loci) of Sd-1 had been lost.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223847
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  • 92
    Digitale Medien
    Digitale Medien
    Thinopyrum bessarabicum: biochemical and cytological markers for the detection of genetic introgression in its hybrid derivatives with Triticum aestivum L. (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 365-370 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Thinopyrum bessarabicum ; Protein-isozyme markers ; Chromosome banding ; Intergeneric hybridization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Thinopyrum bessarabicum (2n = 2x = 14, JJ) with its unique property of salt tolerance provides a potential means for the transfer of this important and complex trait into cultivated wheat through intergeneric hybridization. To accomplish this, diagnostic markers for detecting the presence of Th. bessarabicum chromosomes in a wheat background have to be established. The C-banded karyotype of Th. bessarabicum distinctly identifies individual Th. bessarabicum chromosomes and separates them from those of Triticum aestivum. Also, seven protein/isozymes, i.e., malate dehydrogenase, high-molecular-weight glutenin, Superoxide dismutase, grain esterase, glutamate oxaloacetate transaminase, β-amylase and α-amylase, were identified as being positive markers specific to Th. bessarabicum; these were also expressed in the T. aestivum/Th. bessarabicum amphiploid. These diagnostic biochemical markers could be useful in detecting and establishing homoeology of Th. bessarabicum chromosomes in T. aestivum/Th. bessarabicum intergeneric hybrid derivatives.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222103
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  • 93
    Digitale Medien
    Digitale Medien
    Genetics of gliadins coded by the group 1 chromosomes in the high-quality bread wheat cultivar Neepawa (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 389-399 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Gliadin loci ; Group 1 chromosomes ; Recombination ; Electrophoresis ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The inheritance and biochemical properties of gliadins controlled by the group 1 chromosomes of the high-quality bread wheat cultivar Neepawa were studied in the progeny of the cross Neepawa x Costantino by six different electrophoretic procedures. Chromosome 1B of Neepawa contains two gliadin loci, one (Gli-B1) coding for at least six ω- or γ-gliadins, the other (Gli-B3) controlling the synthesis of gliadin N6 only. The map distance between these loci was calculated as 22.1 cM. Amongst the chromosome 1A gliadins, three proteins are encoded at the Gli-A1 locus whereas polypeptides N14-N15-N16 are controlled by a remote locus which recombines with Gli-A1. Six other gliadins are controlled by a gene cluster at Gli-D1 on chromosome 1D. Canadian wheat cultivars sharing the Gli-B1 allele of Neepawa were found to differ in the presence or absence of gliadin N6. The electrophoretic mobilities of proteins N6 and N14-N15-N16 were unaffected by the addition of a reducing agent during two-dimensional sodium dodecyl sulphate polyacrylamid-gel electrophoresis, suggesting the absence of intra-chain disulphide bonds in their structure.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222107
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  • 94
    Digitale Medien
    Digitale Medien
    Characterisation of the wheat Mr 15000 “grain-softness protein” and analysis of the relationship between its accumulation in the whole seed and grain softness (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 589-597 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Grain softness ; Friabilin ; Milling quality ; Triticum aestivum ; Seed storage-proteins
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The Mr 15000 protein associated with water-washed wheat starch granules from soft wheats was shown to be heterogeneous: it could be divided into a fraction containing one or moreα-amylase inhibitor subunits and a fraction largely composed of a previously uncharacterised polypeptide(s) referred to as the “grainsoftness protein” (GSP). The major N-terminal sequence and sequences of peptides derived from protease digests of GSP are reported. An antiserum specific for GSP was used to show that GSP accumulated in both hard and soft wheat grains, but the GSP in soft grains associated more strongly with starch granules than the GSP in hard grains. A positive correlation between grain softness and accumulation of GSP in the seed was demonstrated for a range of cultivars. This differs from the qualitative relationship, based on the isolated starch fraction, between GSP and grain softness that has already been reported. Analysis of wholemeal extracts with the antiserum demonstrated that the accumulation of GSP in the seed was dependent on the short arm of chromosome 5D, which also encodes theHa locus. In addition, examination of near-isogenic lines differing in hardness indicated that the gene(s) controlling GSP was (were) linked with theHa locus. The findings indicate that GSP may be the product of theHa locus and thus be the major factor that determines the milling characteristics of bread wheats.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00838714
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  • 95
    Digitale Medien
    Digitale Medien
    Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 110-115 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Leaf rust ; RAPD ; RFLP ; Triticum aestivum ; Triticum spelta
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Near-isogenic lines (NILs) for the leaf rust resistance gene Lr9 were screened for polymorphisms at the molecular level. RAPD (random amplified polymorphic DNA) primers as well as RFLP (restriction fragment length polymorphism) markers were used. Out of 395 RAPD primers tested, three showed polymorphisms between NILs, i.e., an additional band was found in resistant lines. One of these polymorphic bands was cloned and sequenced. Specific primers were synthesized, and after amplification only resistant lines showed an amplified product. Thus, these primers define a sequence-tagged site that is specific for the translocated fragment carrying the Lr9 gene. A cross between a resistant NIL and the spelt (Triticum spelta) variety ‘Oberkulmer’ was made, and F2 plants were analyzed for genetic linkage. All three polymorphisms detected by the PCR (polymerase chain reaction) and one RFLP marker (cMWG684) showed complete linkage to the Lr9 gene in 156 and 133 plants analyzed, respectively. A second RFLP marker (PSR546) was closely linked (8±2.4 cM) to the Lr9 gene and the other four DNA markers. As this marker maps to the distal part of the long arm of chromosome 6B of wheat, Lr9 and the other DNA markers also map to the distal region of 6BL. All three PCR markers detected the Lr9 gene in independently derived breeding lines and varieties, thus proving their general applicability in wheat breeding programs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222402
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  • 96
    Digitale Medien
    Digitale Medien
    RFLP markers associated with Sr22 and recombination between chromosome 7A of bread wheat and the diploid species Triticum boeoticum (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 1039-1045 
    Details
    ISSN: 1432-2242
    Schlagwort(e): RFLP ; Sr22 ; Triticum aestivum ; T. boeoticum ; Recombination
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Analysis of the bread wheat variety Schomburgk, and related lines in its pedigree, identified RFLP markers associated with the segment of chromosome 7A carrying the Sr22 gene derived from the diploid species T. boeoticum. The distribution of the RFLP markers indicated that at least 50% of 7AS and 80% of 7AL in Schomburgk is of T. boeoticum origin. Evaluation of five sets of nearisogenic lines, backcross lines in 20 different genetic backgrounds and an F2 population segregating for Sr22 demonstrated a very low level of recombination between the 7A chromosomes of T. boeoticum and T. aestivum. Several recombinants carrying Sr22 but with a much reduced segment of T. boeoticum were identified and these may prove useful in the breeding of further varieties with Sr22.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224536
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  • 97
    Digitale Medien
    Digitale Medien
    Biochemical and molecular diagnostics of Thinopyrum bessarabicum chromosomes in Triticum aestivum germ plasm (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 952-956 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Thinopyrum bessarabicum ; Protein/isozyme markers ; In situ hybridization ; Alien disomic additions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Thinopyrum bessarabicum (2n=2x=14, JJ) is a self-fertile salt-tolerant grass species, and its hybridization with Triticum aestivum to achieve the transfer of this attributes has been promoted. For the detection of alien introgression, development of diagnostic markers of Th. bessarabicum chromosomes in the wheat background has emerged as an important aspect in our intergeneric hybridization program. Six proteins/isozymes-high-molecular-weight glutenins, superoxide dismutase, grain esterase, β-amylase, glutamate oxaloacetate transaminase and α-amylase —were identified as positive markers for detecting the presence of Th. bessarabicum chromosomes in the advanced backcross derivatives of T. aestivum/Th. bessarabicum//n* T. aestivum. Fluorescent in situ hybridization further enabled the detection of complete and translocated arms of Th. bessarabicum chromosomes in the T. aestivum background. These diagnostic markers served for tentatively characterizing a distinct set of Th. bessarabicum disomic additions to wheat (2n=44) and have facilitated establishing the homoeology of these added chromosomes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222908
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  • 98
    Digitale Medien
    Digitale Medien
    Identification of wheat-Agropyron cristatum monosomic addition lines by RFLP analysis using a set of assigned wheat DNA probes (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 70-75 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Agropyron cristatum ; Alien addition ; RFLP ; Non-radioactive labelling
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A non-radioactive digoxigenin-labelled DNA method was used successfully to identify RFLP markers in 54 Triticum aestivum cv ‘Chinese Spring’ — Agropyron cristatum (2n=28, genome PPPP) P-genome monosomic addition lines. Southern analysis using a set of 14 DNA probes identifying each homoeologous chromosome arm, combined with two restriction enzymes HindIII and EcoRI, indicated that six A. cristatum chromosomes (1P, 2P, 3P, 4P, 5P and 6P) and five A. cristatum chromosome arms (2PS, 2PL, 5PL, 6PS and 6PL) have been individually added to the wheat genome. The added chromosomes of three lines were Agropyron translocated chromosomes. It was also found that two addition plants possessed an Agropyron-wheat translocation. These results showed that RFLP analysis using the set of assigned wheat probes was a powerful tool in detecting and establishing homoeology of alien A. cristatum chromosomes, or arms, added to wheat, as well as in screening the alien addition material. The creation of the monosomic addition lines should be useful for the transfer of disease-resistance genes from A. cristatum to wheat.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226985
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  • 99
    Digitale Medien
    Digitale Medien
    Cytologically based physical maps of the group 3 chromosomes of wheat (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 780-782 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Physical mapping ; RFLP ; Cereals ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Cytologically based physical maps for the group 3 chromosomes of wheat were constructed by mapping 25 Triticum aestivum deletion lines with 29 T. tauschii and T. aestivum RFLP probes. The deletion lines divide chromosomes 3A, 3B, and 3D into 31 discrete intervals, of which 18 were tagged by marker loci. The comparison of the consensus physical map with a consensus RFLP linkage map of the group 3 chromosomes of wheat revealed a fairly even distribution of marker loci on the long arm, and higher recombination in the distal region.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00220959
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  • 100
    Digitale Medien
    Digitale Medien
    Chromosomal location of a gene suppressing powdery mildew resistance genes Pm8 and Pm17 in common wheat (Triticum aestivum L. em. Thell.) (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 38-40 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key wordsSuppressor gene ; Powdery mildew resistance ; Gene location ; Triticum aestivum ; Secale cereale ; Monosomic analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The chromosomal location of a suppressor for the powdery mildew resistance genes Pm8 and Pm17 was determined by a monosomic set of the wheat cultivar Caribo. This cultivar carries a suppressor gene inhibiting the expression of Pm8 in cv Disponent and of Pm17 in line Helami-105. In disease resistance assessments, monosomic F1 hybrids (2n=41) of Caribo × Disponent and Caribo × Helami-105 lacking chromosome 7D were resistant, whereas monosomic F1 hybrids involving the other 20 chromosomes, as well as disomic F1 hybrids (2n=42) of all cross combinations, were susceptible revealing that the suppressor gene for Pm8 and Pm17 is localized on chromosome 7D. It is suggested that genotypes without the suppressor gene be used for the exploitation of genes Pm8 and Pm17 in enhancing powdery mildew resistance in common wheat.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050244
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