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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Mechanics of Cohesive-frictional Materials 1 (1996), S. 1-24 
    ISSN: 1082-5010
    Keywords: jointed rocks ; strength criterion ; homogenization method ; yield design ; anisotropy ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A comprehensive three-dimensional formulation for the strength criterion of regular jointed rocks is derived in this paper. The approach is based upon the implementation of the homogenization method of periodic media within the framework of the yield design theory. A rigorous explicit expression of the macroscopic criterion is given as a function of the strength properties of the intact rock and of the joints modelled as interfaces. Attention is focused on the particular case of an infinitely resistance intact rock. It is shown in particular how the relevant velocity jumps for the homogenized medium can be characterized from the introduction of the set of admissible stress vectors acting upon an elementary surface. The results make it possible to perform the static and kinematic approaches in order to address the stability analysis of rock masses cut by sets of regular joints, even for the three-dimensional geometries.
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  • 2
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Mechanics of Cohesive-frictional Materials 1 (1996), S. 45-73 
    ISSN: 1082-5010
    Keywords: loose sand ; load controlled triaxial tests ; creep tests ; time dependency delayed mechanical response ; elasto viscoplasticity ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The time dependency of loose sands mechanical behaviour has been experimentally analysed and theoretically interpreted. A series of load controlled triaxial tests, by imposing finite load increments, has been performed. The single load increments are followed by variable time periods, in order to carry out many classical creep tests.According to the authors, the considered time dependency is due to the internal fabric rearrangement of the granular assembly, i.e. to the plastic strain development with time.This mechanical peculiarity is theoretically interpreted by means of an elastoviscoplastic constitutive model. This is a very simple extension of a previous incremental elastoplastic constitutive model and appears to be capable to reproduce experimental data quite well.Finally, the importance of the considered time dependency is underlined, both by considering numerical solutions and in analysing unstable natural and experimental phenomena.
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  • 3
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Mechanics of Cohesive-frictional Materials 1 (1996), S. 25-44 
    ISSN: 1082-5010
    Keywords: shear waves ; spontaneous liquefaction ; stability ; hypoplasticity ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A theoretical study of plane shear waves in a fully saturated granular medium is presented using a hypoplastic constitutive relation for the solid constituent. Dynamic equations for small disturbances about an initial state are derived and analysed with constitutive parameters for Karlsruhe sand. Permanent changes in stresses during the propagation of waves are shown to result in the liquefaction of the material. As follows from the equations, in the general case the propagation is characterized by two different wave speeds, corresponding to the plus or minus sign of the velocity gradient, i.e. to different directions of an instantaneous shear. The values of the wave speeds depend on the initial state (stresses and void ratio) as well as on the orientation of the wave with respect to the principal stresses of the initial stress tensor. If the granular material is loose enough and the initial stress state differs from the hydrostatic one, it is possible that one of the wave speeds becomes imaginary, i.e. physically does not exist. This case means a loss of stability of the body in the sense that a small boundary disturbance results in a flow of the whole mass which cannot be prevented by a boundary control. Such a solution can be viewed as a model of spontaneous liquefaction. The condition obtained for stability is compared with the one derived from the analysis of the second-order work.
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  • 4
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Mechanics of Cohesive-frictional Materials 1 (1996), S. 75-94 
    ISSN: 1082-5010
    Keywords: concrete modelling ; softening plasticity ; fracture energy models ; stress return ; five parameter model ; improved predictors ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Several computational aspects of the fracture energy based softening plasticity model for plain concrete are considered. A need for a more robust stress return strategy is identified, as the basic closest point projection algorithm leads to regions of nonconvergence, associated with zones of high curvature near the compressive meridian close to the apex. The use of an intermediate stress return to an auxiliary surface to establish an improved predictor is proposed, in conjunction with a controlled scaling of stress return increments, ensuring convergence and providing an efficient and robust stress return strategy.
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  • 5
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Mechanics of Cohesive-frictional Materials 1 (1996), S. iii 
    ISSN: 1082-5010
    Keywords: Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 6
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    New York, NY [u.a.] : Wiley-Blackwell
    Mechanics of Cohesive-frictional Materials 1 (1996), S. 95-114 
    ISSN: 1082-5010
    Keywords: multiphase material ; strain localisation ; averaging theories ; pore pressure ; cavitation ; finite element method ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: It is recalled that negative water pressures are of importance in localisation phenomena of fully saturated, undrained samples of dilatant geomaterials. A model to simulate cavitation phenomena connected with such pore water tractions is developed and implemented in a simplified form in a dynamics code for partially saturated porous media. A case of localisation is studied from the onset of the instability up to the full developed shear band. The weak mesh dependence of the maximum effective plastic strain, due to the employed physical model, is also shown.
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  • 7
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    New York, NY [u.a.] : Wiley-Blackwell
    Mechanics of Cohesive-frictional Materials 1 (1996), S. 115-127 
    ISSN: 1082-5010
    Keywords: concrete ; fracture ; uniaxial compression tests ; boundary restraint ; slenderness ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Localization of deformations has been investigated in a series of displacement controlled uniaxial compression experiments. Of main interest are the effects of specimen slenderness and friction between loading platen and specimen. Both effects have a direct influence on the development of localized fracture zones in the specimen. The results indicate that the use of a double layer of teflon with an intermediate layer of grease yields size-independent results as far as the pre-peak stress-strain behaviour and the peak strength are concerned. However, in terms of stress and strain, a significant influence of both the specimen slenderness and the amount of boundary restraint has been observed in the post-peak regime. It is found that the post-peak curves become almost completely identical when they are plotted in terms of nominal stress and post-peak displacement. For any type of loading platen used, the post-peak relative stress-displacement curves are found to be independent of the specimen height. Furthermore, since during post-peak localization relative sliding and movements of larger parts of the specimen are observed, the definition of a unique Poisson's ratio is virtually impossible.
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  • 8
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    New York, NY [u.a.] : Wiley-Blackwell
    Mechanics of Cohesive-frictional Materials 1 (1996), S. 129-144 
    ISSN: 1082-5010
    Keywords: damage ; void growth ; softening ; localisation ; bifurcation ; rupture ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: An extension of the theory of elastic material with voids to the case where the material undergoes an irreversible void growth is presented. The particularity of this theory is that the continuum is described by two kinematic variables: the displacements and the variation of the volume fraction of material in the porous continuum. Motion is controlled by two governing equations, the classical one involving the displacement or stresses and another one that involves the other kinematic variable, similar to the governing equation in heat conduction problems. The degradation of the elastic moduli is described in the model by a damage scalar variable. A simplified model where the damage variable is proportional to the irreversible variation of volume fraction of material is discussed. From the governing equations, it is deduced that the equation which governs the growth of damage involves the second gradient of damage and a material parameter which plays the role of an internal length according to the analysis of strain localisation. The finite element implementation of the theory is briefly presented. The two variables are discretised separately and the form of the equations to be solved is similar to those obtained in coupled thermoelasticity. One dimensional finite element results of strain localisation show that a proper convergence upon mesh refinement is obtained. The equation which governs the irreversible variation of volume fraction (or the damage growth) acts as a localisation limiter.
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  • 9
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    New York, NY [u.a.] : Wiley-Blackwell
    Mechanics of Cohesive-frictional Materials 1 (1996), S. 145-163 
    ISSN: 1082-5010
    Keywords: constitutive model ; hypoplasticity ; failure criterion ; flow rule ; dissipation function ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Failure criterion, flow rule and dissipation function derived from a class of incrementally non-linear, so-called hypoplastic, constitutive equations are presented. Although the hypoplastic model is developed without recourse to some concepts pertinent to pasticity theory, such as failure surface, plastic potential, flow rule and decomposition of deformation into elastic and plastic parts, it is shown how these concepts may be obtained as natural outcomes of the constitutive model. A connection of the hypoplastic model to Truesdell's hypoelasticity theory and of the derived failure criterion and flow rule to their hypoelastic counterparts is made. By defining the plastic strain rate properly, the dissipation surface is obtained based on the principle of maximum entropy production. Furthermore, the plastic potential is constructed with reference to the direction of plastic strain rate. Finally, the failure surface, flow rule and dissipation surface obtained with a specific hypoplastic constitutive equation are presented and compared with experimental results in the literature.
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  • 10
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    New York, NY [u.a.] : Wiley-Blackwell
    Mechanics of Cohesive-frictional Materials 1 (1996), S. 199-218 
    ISSN: 1082-5010
    Keywords: homogenization ; granular material ; yield surface ; elasticity ; plasticity ; modelling ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Using a homogenization framework, this paper proposes a description of the non-reversible behaviour of granular materials. First the basic equations are reviewed and some general elasticity results are presented with a comparison to DEM simulations. The global non-reversible behaviour is introduced by the definition of local conditions of contact stability: loss of contact and sliding. The first consequence is the possible definition of global yield surfaces and thus failure surfaces. These surfaces are compared to the usual plastic criteria. The influence of the different homogenization parameters introduced by the definition of localization and averaging operators is also studied. In the last section, a simulation of non-reversible behaviour is presented. Comparisons to experimental data are made in the case of simple loading paths (isotropic compression, triaxial test) as well as in complex loading histories.
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  • 11
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Mechanics of Cohesive-frictional Materials 1 (1996) 
    ISSN: 1082-5010
    Keywords: Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 12
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    New York, NY [u.a.] : Wiley-Blackwell
    Mechanics of Cohesive-frictional Materials 1 (1996), S. 219-234 
    ISSN: 1082-5010
    Keywords: effective stress ; theory of mixture ; compressibility ; pore-water pressure ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The effective stress concept in geomechanics advocated by Terzaghi is historically reviewed and the mechanical interpretation of this concept is clarified based on the theory of mixtures. In the interpretation of the effective stress concept, both the compressibility of the constituents and the balance of force are taken into consideration. The effectiveness of the effective stress comes from the fact that the descriptions of the effective stress in undrained and unjacketed conditions are approximately equal, although the role of the pore-water pressure under different test conditions is not the same. It is shown that the effective stress concept is also applicable to soft rock. Finally, classical interpretations and various definitions of the effective stresses are critically examined.
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  • 13
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    New York, NY [u.a.] : Wiley-Blackwell
    Mechanics of Cohesive-frictional Materials 1 (1996), S. 165-197 
    ISSN: 1082-5010
    Keywords: creep ; dilatancy ; damage ; stability ; failure ; galleries ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: An analysis of stability of rectangular-like galleries or caverns is developed from the point of view of creep, creep failure and short-time failure. The initial stress distribution around the cavern just after excavation is obtained with an exact elastic solution. Further this solution is used in conjunction with an elastic/viscoplastic non-associated constitutive equation to determine first the domains around the excavation where the rock becomes dilatant, where compressible, and where a short-time failure is expected. This constitutive equation is further used to determine the creep of the rock around the opening, and where and when a creep failure is to be expected due to excessive dilatancy. It is shown that the location of the incipient creep failure depends on the stress concentration due to the presence of the ‘corners’, on the possible elongated shape of the cavern, and also to the stress concentration induced by the far field stresses. This location also depends on the internal pressure and on depth, and it can be determined quite accurately. Thus the location of incipient creep damage depends on a variety of parameters and the determination of this exact location is very important, and is described in this paper. It is shown that the evolutive damage is spreading mainly in the direction of minimum far field stresses or in the direction of greater elongation of the cross-section. The same constitutive equation allows us to determine the creep convergence (or divergence) of the walls, where this creep is quite fast and when for the first time the incipient creep failure due to dilatancy is to be expected. This timing depends primarily on the magnitude of octahedral shear stress. The time up to creep failure is shorter if this stress is larger (close to the short-term failure value), but tends towards infinity if the stress is relatively small (close but still above the compressibility/dilatancy boundary). Once the various failure modes are well understood the orientation and magnitude of far field stresses can be determined by this analysis if not known a priori. Also, once the location of losing the stability and that of the volume of rock involved in fast creep and creep failure are determined, one can suggest the optimal design of a support. The way in which the stress variation is influencing the above mentioned problem will be discussed in forthcoming papers. While the authors recognize the importance of the pre-existing rock discontinuities in the overall cavern stability, it was thought that a better understanding of this stability starts from analyzing a rock without such pre-existing discontinuities. The examples are given for rock salt.
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  • 14
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    Mechanics of Cohesive-frictional Materials 1 (1996), S. i 
    ISSN: 1082-5010
    Keywords: Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 15
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 357-357 
    ISSN: 1082-5010
    Keywords: Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 16
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 301-320 
    ISSN: 1082-5010
    Keywords: theory of porous media ; micropolar grain rotations ; liquid-saturated cohesive-frictional granular elastoplastic skeleton materials ; single-surface yield function ; non-associated flow ; shear band localization ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Elastoplastic deformations of cohesive-frictional liquid-saturated granular solid materials can be described by use of a macroscopic continuum mechanical approach within the well-founded framework of the theory of porous media (TPM). In the present contribution, the TPM formulation of the skeleton material is extended by micropolar degrees of freedom in the sense of the Cosserat brothers. Proceeding from two basic assumptions, material incompressibility of both constituents (skeleton material and pore liquid) and geometrically linear solid deformations, the non-symmetric effective skeleton stress and the couple stress tensor are determined by linear elasticity laws. In the framework of the ideal plasticity concept, the plastic yield limit is governed by a smooth and closed single-surface yield function together with non-associated flow rules for both the plastic strain rate and the plastic rate of curvature tensor. Fluid viscosity is taken into account by the drag force.The inclusion of micropolar degrees of freedom, in contrast to the usual continuum mechanical approach to the TPM, allows, on the one hand, for the determination of the local average grain rotations and, on the other hand, additionally yields a regularization effect on the solution of the strongly coupled system of governing equations when shear banding occurs. However, in the framework of the original TPM formulation of fluid-saturated porous materials, the inclusion of the fluid viscosity alone also yields a certain regularization on shear band computations. The numerical examples are solved by use of finite element discretization techniques, where, in particular, the computation of shear band localization phenomena is carried out by the example of the well-known base failure problem of geotechnical engineering. © 1997 John Wiley & Sons, Ltd.
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  • 17
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 321-337 
    ISSN: 1082-5010
    Keywords: thermal-softening behaviour ; transient evolution ; corner flow ; analytical solution ; convergence-confinement concept ; thermomechanical interaction ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A closed-form solution of deep tunnel subject to an internal pressure and to an axisymmetrical time-dependent temperature field is presented. The material is supposed to have a thermal-softening behaviour, the cohesion decreasing with the temperature. The thermal expansion generates plastic zones with face flow and corner flow, which can coexist and interact. The explicit character of the solution allows rigorous demonstrations of the evolution of such plastic zones, as well as other interesting and fundamental properties of the thermoplastic behaviour of deep tunnels. On the other hand, the causal relationship between the thermomechanical loading and the structural response (convergence, extension of rupture zones) is rendered transparent, thanks to the simplicity of the analytical solution. The consequence of thermal-softening is clearly shown by comparison with the analytical solution for a constant cohesion previously established. Quantitatively, its importance is illustrated by a restricted parametric study, to which the analytical solution is ideally suited. © 1997 John Wiley & Sons, Ltd.
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  • 18
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 339-356 
    ISSN: 1082-5010
    Keywords: biaxial tests ; combined stresses ; compressive strength ; engineering materials ; failure ; failure surfaces ; mathematical models ; tensile strength ; triaxial tests ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A general, three-dimensional failure criterion is presented. This criterion is formulated in terms of the first and third stress invariants of the stress tensor, and it involves only three independent material parameters. Although these parameters interact with one another, each parameter corresponds to one of three failure characteristics of material behaviour. These material parameters may be determined from any type of strength test, including the simplest possible, such as uniaxial compression and tension tests or biaxial tests for materials with cohesion and tensile strength, and by triaxial compression tests for materials without tensile strength. The procedure for determination of the three material parameters is demonstrated and comparisons between the failure criterion and experimental results are presented for different types of engineering materials. © 1997 John Wiley & Sons, Ltd.
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  • 19
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 1-26 
    ISSN: 1082-5010
    Keywords: anisotropy ; failure criterion ; schist ; sedimentary rock ; rock joints ; laboratory testing ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper is devoted to the assessment of some representative failure criteria in the framework of modelling the failure behaviour of strongly anisotropic geomaterials. Experimental data concerning the failure behaviour of a typical strongly anisotropic rock; the schist of Angers are first presented. Nine widely used failure criteria are then selected and classified into three groups, the mathematical continuous models, the empirical continuous models and the discontinuous weakness planes based models. This classification is made up according to the main assumptions and techniques used in each criterion to describe the strength anisotropy. The calibration of each one is carried out with respect to the laboratory data of Angers schist. Qualitative and quantitative comparisons between the selected criteria and with the experimental data are provided. © 1998 John Wiley & Sons, Ltd.
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  • 20
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 89-103 
    ISSN: 1082-5010
    Keywords: transversely isotropic material ; elliptic paraboloid failure surface ; strength differential effect ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A coordinate-free formulation of a failure criterion for transversely isotropic solids is proposed. In the three-dimensional stress space the criterion is represented by an elliptic paraboloid. The anisotropic form of the proposed criterion is based on generalization of the second invariant of the deviatoric stress and of the mean stress obtained through the introduction of a unique fourth-order tensor. For isotropic conditions, the criterion reduces to the Mises-Schleicher failure condition. It is shown that the criterion satisfactorily predicts the strength anisotropy of transversely isotropic rocks subjected to an axisymmetric stress state. The procedure for the identification of the parameters of the criterion from a few simple laboratory tests is outlined. © 1998 John Wiley & Sons, Ltd.
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  • 21
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 27-39 
    ISSN: 1082-5010
    Keywords: creep ; effective spring concept ; Kelvin chain model ; load bearing volume ; micromechanical model ; relaxation ; softening spring ; solidification ; tension softening ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: An analytical constitutive model similar to the Kelvin chain rheological model associated with solidification theory, is developed for time-dependent tension softening of ageing materials like concrete. The stiffness of spring elements is allowed to vary with time via a function of load bearing volume fraction as in the solidification theory. The development of cracks reduces the load bearing volume fraction with time, so that the overall behaviour of springs is a softening type. A reduction in the load bearing volume with time ensures a gradual reduction in the spring stiffness without violating prescribed retardation times. In some circumstances, depending on the boundary conditions, the stress in a structure can remain unchanged over a period of time. During this period, any cracks in the structure will continue to experience an increased opening due to creep. In other parts of the structure, again depending on the boundary conditions, the crack opening displacements may remain unchanged over a period of time, so that the stress will relax over these parts of the structure. In a large concrete structure, creep and relaxation may be taking place simultaneously in different parts or in the same part but at different times. There is thus a need for a visco-elastic tension softening model for ageing concrete that will cater for both creep and relaxation. © 1998 John Wiley & Sons, Ltd.
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  • 22
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 41-63 
    ISSN: 1082-5010
    Keywords: elastoplasticity ; damage ; bonded geomaterials ; triaxial testing ; intact and remoulded specimen ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In order to model the various phenomena which govern the mechanical response of bonded geomaterials under monotonic loadings, an elastoplastic model coupled with an elastic model with damage was developed, taking into account both the frictional and cohesive aspects of these materials. First, the principles at the base of the model are presented, as well as the physical meaning of the parameters which were used in the elastic model with damage. In order to illustrate the capabilities of the model to reproduce the mechanical behaviour of bonded geomaterials, we simulated triaxial tests on various materials: a deep cemented clay, whose heterogeneity from one specimen to another appeared mainly due to the calcium carbonate content; an assembly of sintered glass balls; and an artificially cemented sand. In this last example, various initial mean stresses allowed us to enlighten the brittle-ductile transition which was modelled by introducing the mean confining pressure in the damage evolution law. We demonstrated that the parameters of each part of the model could be adapted to reproduce the observed general trends. For weak bonds, the elastoplastic part played the predominant role, whereas for strong bonds, the elastic part with damage governed the mechanical behaviour. © 1998 John Wiley & Sons, Ltd.
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  • 23
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 65-87 
    ISSN: 1082-5010
    Keywords: elastoplasticity ; sand ; constitutive modelling ; controllability ; instability ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The paper investigates the possibility of the occurrence of a multiplicity of homogeneous responses of soil specimens under the same incremental loading, and the associated non-controllability of the imposed loading path.It is shown that for non-associated elastoplastic strain-hardening constitutive laws, such a possibility exists even in the hardening regime. Only when the stiffness matrix is positive-definite is the incremental solution unique for any loading programme.A particular constitutive model is chosen and the shape of the loci of non-uniqueness is calculated for various loading programmes. It is shown that the conditions for the occurrence of a shear band and of a peak of the deviator stress in a triaxial undrained test can be seen as special cases of the theory presented. Accordingly, the so-called ‘instability line’ concept in undrained conditions is generalized. © 1998 by John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 105-125 
    ISSN: 1082-5010
    Keywords: localization ; non-local damage ; Perzyna viscoplasticity ; mesh adaptivity ; Arbitrary Lagrangian-Eulerian technique ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The computational modelling of localization of deformation in cohesive-frictional materials must be carried out in a proper, accurate and efficient manner. A proper solution can be obtained by using an enriched material description such as a non-local damage model or Perzyna's viscoplasticity model. However, still a large number of finite elements is needed for an accurate description of the localization zone. To improve efficiency, mesh adaption is applied here by means of the Arbitrary Lagrangian-Eulerian (ALE) technique. The ALE technique must typically be applied in combination with an enriched material model. Otherwise, the use of a standard model gives a zero-width solution of the localization zone and therefore remeshing results in a continuing decrease of finite element size. Implementation of this method requires the addition of convective forces in the equations of motion, transport of the state variables and the formulation of a remeshing strategy. The remeshing strategy heavily determines the success of the ALE method. Three examples of wave propagation have been treated and it is shown that a suitable remeshing strategy in combination with an enriched material model leads to proper, accurate and efficient analyses of the localization process. © 1998 John Wiley & Sons. Ltd.
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  • 25
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 127-153 
    ISSN: 1082-5010
    Keywords: constitutive equations ; post-localization behaviour ; large strain ; interface model ; bifurcation ; shear band ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper addresses developments on a constitutive model able to describe the post-localized behaviour of structures composed by geomaterials. The behaviour of the shear band is defined by means of a specific non-linear constitutive equation in the framework of large strain. Concept of ‘consistency’ of this model with the CLoE model from which it is derived at the onset of localization is discussed. During the post-localization regime this model evolves in a specific way by introducing the concept of critical void ratio. After some recalls about the CLoE framework and about a Rice bifurcation analysis involving CLoE models, the basic concepts of the Daphnis model are introduced. The last part of this paper is devoted to numerical results on an initially homogeneous frictionless biaxial test. The behaviour of the sample is modelled by means of a plane Mohr Coulomb model defined in the CLoE framework and its associated Daphnis model is used to characterize the specific behaviour of the shear band in the post-localization regime. © 1998 John Wiley & Sons, Ltd.
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  • 26
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 155-180 
    ISSN: 1082-5010
    Keywords: reinforced concrete ; bond model ; plasticity ; interface ; yield surface ; modelling ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The development of an interface, non-associative, plasticity model for bond between ribbed, steel bars and concrete is discussed. The model relates average local slip and radial dilation to average bond shear stress and radial confinement stress. The model partially accounts for the response of the damaged, finite-thickness region around the bar - the bond zone. The model is developed for standard steel bars that are initially unstrained. With simplifying assumptions, data for the components of a plasticity law are extracted from a key set of experimental results. In this paper, we emphasize the development of an expression for the yield surface for monotonic loading. While the forms of the model's components are empirically derived, they qualitatively reflect the mechanics of the mechanical interaction of ribbed bars with the adjacent concrete. A characteristic length, related to the rib pattern, helps quantify this interaction. The mechanics of the bond are difficult to characterize in a simple form, but the calibrated model only requires four physical properties and reproduces with acceptable accuracy experimental results with various levels of radial confinement stress. Model refinements are suggested for future work. © 1998 John Wiley & Sons, Ltd.
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  • 27
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 181-205 
    ISSN: 1082-5010
    Keywords: cemented ; carbide ; finite ; element ; compaction ; sintering ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: We have developed a tool for simulating the press cycle and the sintering cycle of cemented carbides. A constitutive model for compaction and tensile fracture has been implemented in the explicit FE programme LS-DYNA2D, and it has been tuned to experiments of hydrostatic compression, uniaxial strain and conventional triaxial compression. The programme simulates the complete compaction cycle in a robust manner. Friction against the tooling is properly taken into account. We have further developed a constitutive model for the stress-free solid-state sintering to also account for a mechanical stress field. The sintering model is based on physical concepts, diffusion parameters, free surface energy, dimensions of carbide grains, etc. of the constituents, and was tuned to successfully simulate the unloaded and loaded sintering of simple specimens. The models of compaction and sintering have a common set of state variables, such that after the compaction cycle, and at raising the temperature the sintering starts out with proper account of the residual stress field. Finally, the potential of the model is demonstrated by simulating the production cycle of a simple WC-Co tool piece until sintering to 75% of full density. The final geometrical shape of the specimen is compared to experimental data. © 1998 John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 229-256 
    ISSN: 1082-5010
    Keywords: finite element method ; viscous constitutive equations ; time-discrete scheme ; stability ; superstability ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The general framework of the paper deals with the finite element modelling of mechanical problems involving viscous materials such as bitumen or bituminous concrete. Its aim is to present a second-order-accurate discrete scheme which remains unconditionally superstable when used for the time discretization of the linear and non-linear viscoelastic constitutive equations considered. After stating the space- and time-continuous mechanical problem we focus on the time discretization of these equations, considering three different schemes. For both of them sufficiently small values of the time step are required in order to ensure the superstability, whereas the third remains unconditionally superstable. Eventually, some numerical results are presented. © 1998 John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 257-276 
    ISSN: 1082-5010
    Keywords: impacts ; structural damage ; discrete element method ; dynamic loading ; scabbing ; perforation ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Impacts of rigid spherical nose shaped missiles on concrete beams are simulated with a discrete element method. The history of the structural damage of the beam is followed from the very first crushing and fracturing processes to the ultimate fragmentation step. Spalling, scabbing, penetration and perforation events are characterized and compared with real test cases of the civil engineering industry. It is found that these phenomena depend not only on the kinetic energy of the missile as predicted by some recent empirical laws but also on its momentum. This means that the dynamic impact loading time is determinant in the scabbing and perforation thicknesses. When the numerical data is plotted against the empirical rules determined from experimental data, the fit is good although the model is two dimensional. © 1998 John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 207-228 
    ISSN: 1082-5010
    Keywords: jointed rock masses ; stability ; failure ; yield design ; homogenization ; strength anisotropy ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Conceived as a potential alternative to the methods usually employed for evaluating the stability of jointed rock masses, the homogenization approach developed in this paper stems from the intuitive idea that, from a macroscopic point of view, a rock mass intersected by a regular network of joint surfaces may be perceived as a homogeneous continuum. The failure criterion of such an equivalent medium is theoretically determined from the knowledge of the failure conditions of the individual constituents, namely the intact rock matrix and joint interfaces. Owing to the existence of privileged material directions associated with the joint orientation distribution, this criterion turns out to be of the anisotropic frictional type, as shown by the closed-form expression obtained in the particular case of two mutually orthogonal joint sets. This criterion is then applied to the investigation of two illustrative engineering problems: the calculation of the load-bearing capacity of a shallow foundation on a jointed rock half-space and the stability analysis of an excavation. Both problems are handled by means of the kinematic method of yield design, making use of the previously determined macroscopic failure condition. The upper bound estimates thus derived appear to be significantly better than those obtained from a direct analysis in which the intact rock matrix and joints are considered separately. In spite of some limitations regarding its range of applicability, which are underlined throughout the paper, the homogenization approach may constitute an appropriate general framework for the design of densely jointed rock structures. © 1998 John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 277-290 
    ISSN: 1082-5010
    Keywords: experimental analysis ; slabs on soil ; reinforced concrete ; steel fibres ; fracture energy ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The cracking control of plain concrete slabs on soil foundation requires the execution of joints with mechanisms of load transfer between adjacent panels. These joints increase the construction costs and, often, are the source of local damage and loss of service performance. Slabs reinforced with steel wire mesh have been used in order to increase the load-carrying capacity and to enhance the cracking control. However, the use of this conventional reinforcement increases the costs, mainly due to labour time spent on the arrangement and positioning of the reinforcement.Fibre-reinforced concrete is a recent material well fitted for applications in industrial floors on soil foundation. The cost of fibres is compensated by a faster construction process and a reduction in the number of expansion joints. The fatigue, impact and flexural strength are significantly improved when steel fibres are added to the concrete mix.The work developed aims to contribute to the on going research effort to clarify the behaviour of fibre-reinforced concrete slabs on soil foundation. For this purpose, an experimental and numerical investigation were carried out. The present article deals basically with the experimental work developed, describing the tests performed and discussing the main results obtained. © 1998 John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 291-303 
    ISSN: 1082-5010
    Keywords: poroelasticity ; isotropic damage ; brittle geomaterials ; enhanced consolidation ; saturated geomaterials ; computational modelling ; indentation of geomaterials ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper examines consolidation behaviour of saturated geomaterials with a matrix component which is susceptible to damage. Finite-element-based computational model accounts for the alteration in both the deformability and permeability characteristics of the porous material due to damage evolution. The isotropic damage criteria governing the evolution of elastic stiffness and hydraulic conductivity parameters are characterized by the dependency of the damage variable on the distortional strain invariant. The computational procedure is utilized to evaluate the extent to which the time-dependent axisymmetric indentation behaviour of a rigid circular punch on a poroelastic half-space can be influenced by the damage evolution in the porous skeleton. © 1998 John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 305-322 
    ISSN: 1082-5010
    Keywords: strain localization ; finite element ; mesh sensitivity ; regularization ; gradient plasticity ; multilayered concrete beams ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A gradient-dependent plasticity theory is applied to finite element solutions of static strain localization problems. Assuming weak satisfaction of constitutive equations, a multilayered beam finite element with a mixed character is developed. The plastic strain field is discretized in addition of the displacement field. A consistent Newton-Raphson algorithm for this approach is presented. Some examples are carried out in order to illustrate the approach and verify the performances of the element. © 1998 John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 365-365 
    ISSN: 1082-5010
    Keywords: Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: No Abstract
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 323-342 
    ISSN: 1082-5010
    Keywords: concrete fracture ; computational modelling ; continuum damage mechanics ; localization ; regularization ; gradient enhancement ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Classical continuum damage theory for quasi-brittle fracture exhibits an extreme sensitivity to the fineness and orientation of the spatial discretization in finite element simulations. This sensitivity is caused by the fact that the mathematical description becomes ill-posed at a certain level of accumulated damage. The ill-posedness can be removed by the use of a gradient-enhanced damage model. In this model, higher-order deformation gradients give rise to a non-local effect, which regularizes the localization of deformation and thus renders numerical analyses mesh-objective.The mesh objectivity of the gradient-enhanced damage approach is demonstrated by the application to two concrete fracture experiments: a double-edge notched bar subjected to a uniaxial, tensile load and a single-edge notched beam under anti-symmetric four-point loading. Both the initiation and the propagation of damage can be simulated. Particularly the latter aspect calls for an appropriate definition of the strain measure which governs the evolution of damage. © 1998 John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 3 (1998), S. 343-364 
    ISSN: 1082-5010
    Keywords: concrete modelling ; microplane model ; anisotropic damage ; consistent linearization ; localization analysis ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The paper addresses the microplane model in the context of localization analysis. Capable of reproducing experimental results of concrete specimens, the microplane model includes anisotropic damage in a natural and conceptually simple and explicit way. However, the efficiency of former microplane implementations suffers from the expense of the solution procedure being based on the secant stiffness method. Within this paper, the macroscopic constitutive equation derived by kinematically constraining the microplane strains to the macroscopic strain tensor is consistently linearized resulting in quadratic convergence of the Newton-Raphson iteration for the equilibrium equations. A fully three-dimensional model will be presented and linearized incorporating the two-dimensional case in a natural fashion. Furthermore, the localization criterion is analysed, indicating locally the onset of localization in terms of the acoustic tensor. Several examples demonstrate the features of the microplane model in predicting the material behaviour of concrete in tension and compression as well as in shear. © 1998 John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 1 (1996), S. 235-249 
    ISSN: 1082-5010
    Keywords: boundary element method ; concrete ; fracture mechanics ; fictitious crack model ; crack growth ; contact mechanics ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A boundary element formulation for the analysis of pullout behaviour of an anchor bolt embedded in concrete is presented. The pullout analysis involves modelling two different bodies (i.e. anchor head and concrete) which are in contact over a certain region. The fracture of concrete is represented by the fictitious crack model (FCM) in which the fracture zone is replaced by applying closing forces on both crack surface. The FCM in conjunction with the boundary element method (BEM) allows the simulation of crack growth in concrete in a straight forward manner without remeshing. The crack path need not be known in advance as it is calculated during the iteration process. The numerical results obtained are compared with the round-robin analysis of pullout test proposed by RILEM TC 90-FMA.
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    Mechanics of Cohesive-frictional Materials 1 (1996), S. 251-271 
    ISSN: 1082-5010
    Keywords: constitutive law ; plasticity ; hypoplasticity ; asymptotic state ; limit surface ; sand ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A hypoplastic theory for granular materials developed by Gudehus and Bauer is discussed. The description of asymptotic states is of particular interest. Three forms of asymptotic states are defined. Useful criteria to describe the tensorial part of the constitutive relation is developed for one of them, namely for the critical states. The terms proposed by Wu are correlated to the well-known formulations of elastic plastic theory: the Drucker/Prager model and the yield condition by Matsuoka/Nakai. The suitability of the Matsuoka/Nakai criterion for critical states is discussed.Specification of tensorial functions follows in two steps. First the hypoplastic Drucker/Prager model is developed, and then the limit condition by Matsuoka/Nakai is implemented. The resulting tensorial functions require the critical friction angle as the only material constant. The limit condition in critical states obtained from the hypoplastic law coincide with the one by Matsuoka/Nakai. A more comprehensive hypoplastic constitutive relation based on these new tensorial functions is discussed and applied to simulations of element tests. These numerical results are compared with experimental results for sand.
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    Mechanics of Cohesive-frictional Materials 1 (1996) 
    ISSN: 1082-5010
    Keywords: Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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    Mechanics of Cohesive-frictional Materials 1 (1996), S. 273-294 
    ISSN: 1082-5010
    Keywords: homogenisation ; layered media ; non-linear analysis ; plasticity ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: In this article attention is given to the homogenisation of periodic layered materials. Based on the assumption of a homogeneous state of stress and strain in each layer, a novel matrix formulation capable of representing the elastic behaviour of the composite material is established. The matrix formulation yields a much clearer implementation of linear elastic homogenisation algorithms and a relatively straightforward extension to inelastic behaviour. The theory of plasticity, which is adopted to describe the inelastic behaviour, follows modern concepts, including a unconditionally stable implicit Euler backward return mapping, a local Newton-Raphson method and a consistent tangent stiffness matrix. A comparison between the homogenised continuum and the standard continuum with an exact discretisa tion of the geometry of the composite shows excellent agreement, both in the presence of elastic and inelastic material behaviour.
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    Mechanics of Cohesive-frictional Materials 1 (1996), S. 295-304 
    ISSN: 1082-5010
    Keywords: creep ; effective spring concept ; micromechanical model ; relaxation ; rheological model ; softening-type spring ; tension softening ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A methodology that combines a rheological model for viscoelastic behaviour with a micromechanical model for tension softening through the effective spring concept is developed for materials exhibiting both tension softening and time-dependent behaviour. Spring elements with softening characteristics and dashpot with varying viscosity are used for this purpose. The characteristics of the spring elements and dashpot are obtained from a micromechanical model which relates the microstructure of the material to its tension softening response. The softening-type springs ensure gradual reduction in stiffness, whereas the dashpot with varying viscosity ensures that the retardation and relaxation times equal prescribed values. In this way, both the stress and crack opening are allowed to vary with time to reflect the real behaviour of a time-dependent tension softening material. The methodology is illustrated on the simple Poynting- Thompson rheological model, without restricting its application to more sophisticated models.
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    Mechanics of Cohesive-frictional Materials 1 (1996), S. 305-319 
    ISSN: 1082-5010
    Keywords: concrete structures ; alkali-aggregate reaction ; plasticity ; finite elements ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: In this paper a simple continuum theory, as recently proposed by Pietruszczak (Int. J. Computers Struct., 58, 1093-1099 (1996)), is applied to describe the mechanical effects of the alkali-aggregate reaction in concrete structures. The formation of alkali- silica gel leads to a progressive expansion of the material. The progress in the reaction is assumed to be coupled with the degradation of mechanical properties, the latter described within the framework of elastoplasticity. The paper discusses the details of the numerical implementation of the constitutive model, including an implicit integration scheme and the computation of an updated tangent operator. Numerical examples are provided to assess the performance of the proposed algorithm. The formulation is then used to analyse the junction between the right-wing dam and the water intake structure of the Beauharnois powerhouse situated in Quebec (Canada). A non-linear 3D finite element analysis is performed simulating the time history of the deformation due to continuing reaction.
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    Mechanics of Cohesive-frictional Materials 1 (1996), S. 349-366 
    ISSN: 1082-5010
    Keywords: damage ; fracture energy ; homogenisation ; masonry ; mesh dependence ; unilateral effect ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: By considering masonry as a composite material, its mechanical properties are obtained by taking into account the properties of the components (bricks and mortar) through a homogenisation technique. To describe the behaviour of the material components a unilateral damage model is proposed. This model, based on the introduction of three damage variables, describes the behaviour of brittle materials subjected to alternating tensile-compressive cyclic loads. The model is applied to the simulation of tests on masonry panels and miniaturised walls; numerical results are discussed and successfully compared with experimental data.
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    Mechanics of Cohesive-frictional Materials 1 (1996), S. 321-347 
    ISSN: 1082-5010
    Keywords: damage ; dilatancy ; failure-modes ; fractures ; localization ; shear ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A model is presented based on the non-local damage theory. It sets out to describe the behavior of concrete under free-variable loads, which are constant in sign. Its purpose is to analyze shear behavior and high strain-gradient localized problems, and it takes Mazar's model as a starting point with reference to the basic idea of a scalar isotropic non-local damage controlled by principal tensile strains. In addition, the other two main features are an internal variable denoted to the control or reversible volumetric expansion in compression, and irreversible strains aimed at modelling crushing in compression and cracks both in tension and compression. As a consequence, induced-anisotropy, dilatancy and path-dep endency can be reproduced. In particular, the modelling of micro- and macrocracks makes it possible to capture mixed-mode cracking as well as aggregate interlock, which requires a residual stiffness to guarantee the transmission of transversal and normal stresses for assigned slips. The model requires the knowledge of the material response in uniaxial tension and compression, and biaxial compression tests which can be introduced directly by adopting experimental curves, or by means of a reduced number of parameters. The effectiveness of the model is shown through comparisons with several sets of experimental tests on both small specimens, assumed to be homogeneous, and boundary value problems.
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    Mechanics of Cohesive-frictional Materials 1 (1996), S. 367-383 
    ISSN: 1082-5010
    Keywords: plasticitity ; isotropy ; Mohr-Coulomb ; consistent linearization ; algorithmic tangent stiffness ; multisurface plasticity ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: In this paper, we discuss the efficient treatment of yield criteria that are of the Mohr-Coulomb type for elastic and plastic isotropy. On the basis of the fully implicit method, we derive the explicit expression for the integrated stress along with a flow rule that represents volumetric non-associativity. The integration algorithm covers all the possible cases of regular, corner and apex solutions including the suitable indicator for each case. We also establish the consequent consistently linearized tangent stiffness modulus tensor, which is shown to appear in the form of an additive modification of the continuum tangent stiffness tensor. The convergence properties of the consistent tangent stiffness tensor are compared with its feasible approximations. The results indicate the strongly sensitivity to the proper treatment of the corner conditions at the establishment of the ATS-tensor.
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    Mechanics of Cohesive-frictional Materials 1 (1996) 
    ISSN: 1082-5010
    Keywords: Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Mechanics of Cohesive-frictional Materials 1 (1996), S. 385-403 
    ISSN: 1082-5010
    Keywords: granular flow ; constitutive equation ; simple fluid ; friction ; collision ; microstructural model ; Engineering ; Civil and Mechanical Engineering
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    Notes: The aim of this paper is to examine the possibility of a simple fluid-mechanics treatment of rapid dense granular flows. In other words, we examine whether the constitutive equation can be sought in a simple relationship between the strain-rate and stress tensors. With this aim, we first show that an inclined channel is an appropriate device for providing rheological data. Here we provide a complete rheometrical treatment, which allows to infer the shear-stress/shear-rate curve (for simple shear flows) from the flow-depth/mass-flow-rate curve. Experi-ments performed with glass beads and sand grains revealed an apparent decrease in the shear stress with increasing shear rate. We then demonstrate that this result, although paradoxical, is not unphysical. Moreover, more detailed theoretical analysis shows that the main issues raised by our experiments may be overcome by ‘microstructural’ models. We finally give two examples of models including a single microstructural parameter, which are able to qualitatively account for the main features of our experiments.
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 31-46 
    ISSN: 1082-5010
    Keywords: block structure ; elasticity ; homogenization ; Cosserat continuum ; dynamics ; large deformation ; Engineering ; Civil and Mechanical Engineering
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    Notes: A continuum model for regular block structures is derived by replacing the difference quotients of the discrete equations by corresponding differential quotients. The homogenization procedure leads to an anisotropic Cosserat Continuum. For elastic block interactions the dispersion relations of the discrete and the continuous models are derived and compared. Yield criteria for block tilting and sliding are formulated. An extension of the theory for large deformation is proposed. © 1997 by John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 1-29 
    ISSN: 1082-5010
    Keywords: numerical integration ; constitutive modelling ; frictional materials ; highly non-linear hardening functions ; Engineering ; Civil and Mechanical Engineering
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    Notes: Constitutive relations in elastoplasticity may be formulated in a variety of ways, and different update algorithms may be employed to solve the resulting equations. Several implicit integration schemes, although some not widely used, have been suggested in the last years. Among them, the closest point projection method (CPPM) has proven to be an effective and robust integration scheme. In order to gain maximum control of the stress projection, a two-level CPPM iteration scheme is proposed. The hardening variables are fixed during the stress projection onto consequently fixed yield surfaces, and after the stress projection, new values of the hardening variables are calculated defining new yield surfaces. The update of the hardening parameters which, in general, may be highly nonlinear functions, may be determined by a combination of a Picard Iteration (PI) on the hardening variables and an adaptative order inverse interpolation (AOII) on the difference of subsequent iterations of the hardening variables.The integration scheme has been implemented in a general constitutive driver which has been formulated independent of the selected constitutive model and easily linked to finite element codes. A third stress invariant dependent, cone-cap elastoplastic constitutive model, referred to as the MRS-Lade, with a highly non-linear hardening function has been used to show the applicability of the proposed iteration scheme. Error analyses and accuracy assessment are presented along with some representative test results. © 1997 by John Wiley & Sons, Ltd
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 71-92 
    ISSN: 1082-5010
    Keywords: undrained instability ; static liquefaction ; non-associated plasticity ; loss of uniqueness ; model validations ; very loose sand ; Engineering ; Civil and Mechanical Engineering
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    Notes: The characteristics of the loss of stability associated with the initiation of the static liquefaction phenomenon is obtained using the CJS model within the elastoplasticity framework. This model is based on the characteristic line concept. The determining factors involved in the prediction of this phenomenon and in the modelling process are identified. The importance of the elastic components is highlighted, in particular its non-linearity. The possibilities and the limits of the proposed model to describe the undrained behaviour of very loose sand are examined, taking into consideration the effects of the more or less complex stress history. Finally, the conditions leading to the appearance of the uniqueness domain are indicated. The findings of this theoretical study rely entirely on the experimental data presented in a companion paper, with emphasis on the triaxial tests on anisotropically consolidated samples. © 1997 by John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 93-120 
    ISSN: 1082-5010
    Keywords: loose sands ; load-controlled triaxial tests ; creep tests ; instability ; theoretical interpretation ; strain-rate sensitivity ; Engineering ; Civil and Mechanical Engineering
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    Notes: A series of triaxial load-controlled tests is performed.Finite load increments are imposed. The single load-steps are followed by a time period during which the axial load is kept constant.At low stress levels the mechanical response is stable and characterized by a continuous decrease in strain rate with time. At higher stress levels, the mechanical response changes and, subsequently, the collapse takes place.The collapse is unexpected and occurs at a stress level less than that associated with the steady state, experimentally observed by performing strain triaxial controlled tests.In order to interpret such a behaviour, a theoretical discussion is introduced. This is based on a dynamical reinterpretation of the micromechani cal fabric rearrangement of granular assembly.In particular, the role played by the kinetic energy of the system, as well as that played by the anisotropy of the microstructure, have been analysed. © 1997 by John Wiley & Sons, Ltd. Mech. cohesive-frictional mater. 2, 93-120 (1997)
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 47-70 
    ISSN: 1082-5010
    Keywords: instability ; liquefaction ; laboratory tests ; undrained ; triaxial ; loose sand ; Engineering ; Civil and Mechanical Engineering
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    Notes: The undrained behavior of very loose Hostun RF sand in triaxial compression and extension tests is described. The samples are consolidated isotropically or anisotropically along constant effective stress ratio paths. Very loose sand exhibits partial liquefaction, deviator peak stress at relatively low to very low axial strain, gradual post-peak stress reduction to a small residual value at large strains, sharp loss of effective mean pressure due to generation of large pore pressure and overall volume reduction. The instability line of Lade is examined in the case of extension tests and extended for anisotropic samples. It is shown that monotonic and anisotropic consolidation strongly influences the instability concept. A higher positive anisotropic consolidation level produces a greater slope of the instability line in compression and a reverse trend can occur in extension. Complex stress history can develop a fossilized instability line depending on the amount of axial strain attained. Effective strain ratio increment at peak has an asymptotic stabilization effect. © 1997 by John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 121-163 
    ISSN: 1082-5010
    Keywords: granular materials ; micromechanics ; biaxial tests ; complex loading paths ; micro-macro relations ; fabric anisotropy ; Engineering ; Civil and Mechanical Engineering
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    Notes: This paper is concerned with micromechanics of Schneebeli material specimens composed of wooden roller stacks. Several laboratory tests are carried out to analyse the material behaviour under complex loading conditions, involving loading-unloading cycles and principal axes rotations. In order to characterize micromechanical deformation features and structure evolution, a series of pictures is taken during loading. Pictures are then digitized using a stereo device, obtaining the position of each roller. Starting from these data a number of computer programs, conceived for the purpose, allow us to measure micromechanical variables and to analyse their evolution.In the following, after the description of the devices employed in this research, macromechanical results are analysed to evaluate the reliability of the laboratory model. Then, local variables are introduced and the use of continuum mechanics to describe granular materials behaviour is discussed. Finally, the evolution of local kinematic variables is described, focusing interest on the evolution of specimen anisotropy. © 1997 by John Wiley & Sons, Ltd. Mech. cohesive-frictional mater. 2, 121-163 (1997)
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 165-183 
    ISSN: 1082-5010
    Keywords: elastoplasticity ; pressure sensitive materials ; constitutive modelling ; dilatancy ; implicit integrations ; Engineering ; Civil and Mechanical Engineering
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    Notes: The paper presents the derivation of a fully implicit Newton algorithm for direct integration of constitutive equations, in extended stress-internal variable space, involving hardening or softening of a general dilatant isotropic elastoplastic geomaterial. All relevant derivatives are provided in tensor notation, thus facilitating implementation. The consistent, algorithmic tangent stiffness tensor is derived. The relative accuracy of a template algorithm is assessed on a number of examples by means of iso-error maps. We present a rather simple, one-increment example concerning convergence properties of the Newton iterative scheme at the global, finite element level, associated with the consistent tangent stiffness tensor for integrating the weak form of the equilibrium equations. © 1997 by John Wiley & Sons, Ltd. Mech. Cohesive-frictional mater. 2, 165-183 (1977)
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 185-203 
    ISSN: 1082-5010
    Keywords: fluid-saturated porous media ; enhanced element technology ; Engineering ; Civil and Mechanical Engineering
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    Notes: This contribution is concerned with a new mixed finite element formulation for geometrically linear Terzaghi-Biot type fluid-saturated porous media. To this end, an extended Hu-Washizu type mixed variational principle is presented for fluid-saturated porous continua. Then, a suitable discretization and its implementation are discussed, resulting in an improved element behaviour especially in numerical localization analyses. The intriguing element performance is firstly demonstrated for the case of localization within an elastoplastic compression problem. Finally, an elastoplastic slope stability problem is examined, whereby the new element formulation proves to render more pronounced failure modes as compared with a standard element expansion. © 1997 John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 205-221 
    ISSN: 1082-5010
    Keywords: pollutant diffusion ; advection ; porous media ; surface transfer resistance ; physical modelling ; homogenization ; model classification ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: In the paper, an attempt to model local equilibrium and local non-equilibrium in pollutant migration in porous media is presented. Diffusion is assumed to occur in the pores as well as in the solid porous matrix. The macroscopic models are developed using the homogenization method (asymptotic expansion technique). The resulting macroscopic equations show either a one-concentration field or two-concentration fields or memory effects. The domains of validity of all the presented models are also determined. Local cell problems are provided to compute the effective diffusion tensors. The conditions for the local mass equilibrium assumption to be valid are derived in terms of the dimensionless characteristic parameters. The effect of pollutant source distributions is also investigated. © 1997 John Wiley & Son, Ltd.
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 223-236 
    ISSN: 1082-5010
    Keywords: granular materials ; vortices ; discrete element method ; coherent structures ; proper orthogonal decomposition ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Discrete element models are used to investigate the formation of coherent structures within a deforming granular material. The numerical models predict the formation of coherent vortex-like structures, even when the boundary deformations introduce zero vorticity. We name these structures circulation cells because the particles instantaneously translate and rotate as a rigid body about a common centre. They occur for all the particle shapes and material properties tested. The size of these coherent structures range from approximately 20 to 600 particles, with the largest structures being limited by the test boundaries. Circulation cells are seen to play an important role in granular deformations including the formation of shear bands. © 1997 John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 237-249 
    ISSN: 1082-5010
    Keywords: concrete ; confinement ; dilation ; fibres ; plastics ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Confinement of concrete enhances it strength and ductility by restraining lateral dilation. The accuracy of a confinement model depends on how well it captures the dilation tendency of concrete. In recent years, external confinement of concrete by fibre composites has become increasingly popular for civil infrastructure applications. This includes fibre-wrapping of existing columns or encasement of concrete in a fibre reinforced plastics (FRP) shell. A total of 54-concrete-filled FRP tubes were tested in uniaxial compression under displacement control mode. Full instrumentation of the specimens has allowed the variation of tangent Poisson's ratio for concrete to be captured. The dilation trend of confined concrete is shown to be a function of jacket stiffness. In steel-encased members, once steel yields, confining pressure becomes constant and the jacket renders itself ineffective in containing the dilation of concrete. On the other hand, for linear-elastic materials such as fibre composites, a strain reversal occurs that results in containment of dilation. A method for predicting the dilation is developed that can be easily adopted in any active confinement model. Moreover, a new confinement model for FRP-encased concrete is discussed. © 1997 John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 279-299 
    ISSN: 1082-5010
    Keywords: constitutive model ; hypoplasticity ; plasticity ; grain contacts ; intergranular strain ; strain-space plasticity ; recent history ; small strain ; ratcheting ; Engineering ; Civil and Mechanical Engineering
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    Notes: In order to eliminate ratcheting a so-called intergranular strain has been added to a hypoplastic constitutive model. This additional state variable represents the deformation of the interface layer between the grains. The new concept is outlined and comparisons with and without intergranular strain are presented. Some comments on numerical implementation and determination of material constants are made. A discussion on the uniqueness of the solution and objectivity of the rate of intergranular strain is added. © 1997 John Wiley & Sons, Ltd.
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    Mechanics of Cohesive-frictional Materials 2 (1997), S. 251-278 
    ISSN: 1082-5010
    Keywords: Pietra Leccesse chalk ; laboratory tests ; constitutive modelling ; capillary forces ; partial saturation ; collapsible rocks ; Engineering ; Civil and Mechanical Engineering
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    Notes: Experiments on Pietra Leccese chalk revealed that many of the material parameters describing its mechanical behaviour, such as the Young's modulus, the uniaxial compressive strength and the plastic yield stress, depend strongly on the water saturation and the ensuing capillary suction. The experimental results show that Bishop's generalized effective stress is not by itself adequate to explain the observed behaviour and in particular the spontaneous collapse which may occur upon water injection under stress. An elastoplastic constitutive model for partially-saturated collapsible rocks is proposed where the capillary suction is assumed to affect primarily the material properties related to the intergranular cohesion, such as the elastic stiffness, the plastic tension cut-off and pressure cap parameters. The model builds upon the well- established Mohr-Coulomb theory of elastoplasticity by including a pressure cap and considering the water saturation as an internal variable. In low levels of water saturation, as is often the case in oil reservoir formations, the experimental and theoretical results demonstrate the potential significance of capillary effects on the response of reservoir rocks, a mechanism that has been largely underestimated in the petroleum industry. © 1997 John Wiley & Sons, Ltd.
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    Cell Biochemistry and Function 1 (1983), S. 92-96 
    ISSN: 0263-6484
    Keywords: Blood cells ; leukaemia ; myelodysplasia ; cytochemistry ; neutrophils ; microdensitometer ; lysosomes ; preleukaemia ; maturation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Quantitative cytochemistry of components of blood neutrophil azurophilic granules (myeloperoxidase, chloroacetate esterase, β-glucuronidase, and acid phosphatase) and specific granules (lactoferrin) has been performed by scanning and integrating micro-densitometry in 13 patients with a myelodysplastic syndrome and 11 patients with chronic granulocytic leukaemia. Both patient groups showed a reduction of enzyme activity in azurophilic granules, and also of lactoferrin, consistent with abnormal development of neutrophil granules. These cytochemical changes in blood neutrophils are similar to those found in acute myeloid leukaemia, are consistent with a leukaemic maturation defect, and may be of diagnostic value.
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    Cell Biochemistry and Function 1 (1983), S. 131-140 
    ISSN: 0263-6484
    Keywords: Biochemistry ; polyamines ; putrescine ; spermidine ; spermine ; ornithine decarboxylase ; biosynthesis ; cell proliferation ; oxidized polyamines ; Chemistry ; Biochemistry and Biotechnology
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    Topics: Biology , Medicine
    Notes: The naturally-occurring polyamines exist in the free form, as N-acetyl derivatives and bound to protein. Their biosynthesis is subject to sensitive control, particularly of ornithine decarboxylase. This enzyme may be multifunctional and a key regulatory protein. Studies, principally with selective inhibitors, have elucidated the roles of polyamines in cell proliferation. Oxidized polyamines, in contrast, can be potent mitotic inhibitors. These effects are reviewed in terms of their chemistry and biochemistry. Their principal distinctions are that they can be made or degraded intracellularly, they can associate electrostatically with macromolecules by means of their spaced cationic groups, and these can be readily converted to covalent bonds.
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    Cell Biochemistry and Function 6 (1988), S. 7-12 
    ISSN: 0263-6484
    Keywords: Aging ; ethanol metabolism ; alcohol dehydrogenase ; microsomal functions ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The study of the influence of the age of the animals (13 to 53 weeks) on the rate of ethanol metabolism in vivo and the total activity of liver alcohol dehydrogenase and microsomal ethanol oxidizing system showed a progressive decline with age. These effects were observed concomitantly with a diminution in the content of cytochrome P-450 and microsomal functions related to oxidative and free-radical mediated reactions, namely, NADPH oxidase activity, NADPH-dependent oxygen uptake and NADPH-or t-butyl hydroperoxide-induced chemiluminescence. It is concluded that ageing is accompanied by a diminution in the total oxidative activity of the liver tissue, which would explain the depression in basal and ethanol-induced lipid peroxidation found in the oldest group of rats studied.
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    Cell Biochemistry and Function 1 (1983), S. 168-172 
    ISSN: 0263-6484
    Keywords: Bone ; aerobic glycolysis ; fatty acid oxidation ; cartilage ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The apparent paradox of aerobic glycolysis has been investigated in bone and in cartilage. A new cytochemical procedure for hydroxyacyl dehydrogenase (HOAD) activity showed that the maximal activity of this enzyme in both tissues was equivalent to the maximal activity of glyceraldehyde 3-phosphate dehydrogenase (GAPD). The sum of these activities gave a measure of the maximum amount of acetyl-coenzyme A that could be produced. In these tissues, but not in liver which does not exhibit aerobic glycolysis, this summed value exceeded the maximal activity of succinate dehydrogenase (SDH). Consequently, it suggested that where fatty acid oxidation is sufficient to supply all the acetyl-coenzyme A required for the Krebs' cycle, that derived from fatty acid oxidation may inhibit pyruvate dehydrogenase causing accumulation of pyruvate which must be converted to lactate if pentose-shunt activity is to be maintained.
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    Cell Biochemistry and Function 1 (1983), S. 173-178 
    ISSN: 0263-6484
    Keywords: RNA ; quantitative cytophotometry ; luteal regression ; ovarian 20α-hydroxypregn-4-ene-3-one ; Chemistry ; Biochemistry and Biotechnology
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    Topics: Biology , Medicine
    Notes: Luteal and interstial cell RNA contents and circulating progesterone (P) and 20α-hydroxypregn-4-ene-3-one (20α-OH P) levels were measured during pseudopregnancy in order to characterize relationships between ovarian 20α-OH P secretion and luteal regression. Functional luteolysis, as manifested in depressed P levels, was not associated with concurrent elevations in 20α-OH P. Rather, augmented 20α-OH P levels were evidenced in periovulatory periods at the onset and termination of pseudopregnancy, subsequent to RNA accumulation in both luteal and interstitial compartments. It is postulated that 20α-OH P, the putative inactive metabolite of P, is produced by both ovarian compartments in a cyclic manner and in response to gonadotrophin released in the preovulatory period.
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    Cell Biochemistry and Function 1 (1983), S. 186-186 
    ISSN: 0263-6484
    Keywords: Chemistry ; Biochemistry and Biotechnology
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    Cell Biochemistry and Function 1 (1983), S. 187-187 
    ISSN: 0263-6484
    Keywords: Chemistry ; Biochemistry and Biotechnology
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    Cell Biochemistry and Function 1 (1983), S. 179-185 
    ISSN: 0263-6484
    Keywords: Blood ; forskolin ; protein phosphorylation ; platelets ; release reaction ; secretion ; cAMP ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effects of diterpene forskolin on the human platelet release reaction and on platelet protein phosphorylation were studied. This drug is shown to have the same effects as other agents which increase cAMP levels, namely, it inhibits the secretory response to diverse agonists and causes changes in the phosphorylation of several specific proteins. An increase of the 32P content is seen in the MW 47 000, 24 000 and 21 000 polypeptides while a decrease is observed in the MW 41 000 and 27 000 and 20 000 species. Forskolin also inhibits the changes in protein phosphorylation pattern induced by the powerful platelet secretagogue, thrombin. Our results relate the effects of antagonists of platelet secretion such as prostaglandins more closely to changes in cAMP levels and in protein phosphorylation than to other possible effects of the receptor-ligand interaction, which is by-passed by the use of forskolin. Our results also provide additional evidence involving these changes in the mechanisms which regulate the secretory process in platelets.
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    Cell Biochemistry and Function 8 (1990), S. 31-38 
    ISSN: 0263-6484
    Keywords: Proximal tubule ; Pi depletion ; calcium ; phorbol ester ; gluconeogenesis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Pi depletion of proximal tubule cells isolated from mouse kidney results in a decrease in the cell content of fructose-2,6-bisphosphate and an increase in the rate of gluconeogenesis from pyruvate, malate and succinate. Gluconeogenesis from glycerol is unaffected by Pi depletion. Introduction of fructose-2,6-bisphosphate into the cytosol of ATP-permeabilized cells is accompanied by a fall in gluconeogenesis.The presence of external Ca2+ stimulates gluconeogenesis. When cytosolic Ca2+ is raised to 1·8 μM by permeabilization, the resealed cells still require 2·5 mM Ca2+ in the bathing medium in order to perform gluconeogenesis at the maximum rate. Cells permeabilized in the presence of cAMP show a decreased rate of glucose production. Phorbol ester stimulates gluconeogenesis provided that the phorbol treatment is performed in the absence of Ca2+ ions.It is suggested that Pi depletion may stimulate pyruvate carboxylase activity and facilitate the entry of certain gluconeogenic substrates into mitochondria. It is also proposed that important aspects of the control of renal gluconeogenesis by parathyroid hormone are mediated by protein kinase C.
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  • 70
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    Cell Biochemistry and Function 8 (1990), S. 39-47 
    ISSN: 0263-6484
    Keywords: Fructose-1, 6-diphosphate (FDP) ; heart slices ; GABA-receptors ; Ca++ entry ; Cl- up-take ; gastric ulcers ; serum transaminases ; diuresis ; narcosis ; dependence and withdrawal symptoms ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Fructose-1, 6-diphosphate (FDP) decreases the effect of ethanol on Ca++ entry and inhibits the ethanol-stimulated phosphate efflux in rat heart slices. FDP also inhibits the ethanol-stimulated [36Cl-]-uptake by rat brain microvesicles and affects the isolated GABA-receptor in a way opposite to that of ethanol. The in vivo effects of FDP include a dose-dependent decrease in ethanol-induced gastric ulcers and a decrease in the serum transaminase levels raised by chronic ethanol administration. Other central actions of ethanol such as diuresis, narcosis, dependence and withdrawal symptoms are also counteracted by FDP.
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  • 71
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    Cell Biochemistry and Function 8 (1990), S. 49-56 
    ISSN: 0263-6484
    Keywords: Insulin receptor ; unicellular model system ; Tetrahymena ; hormonal imprinting ; quantitative cytofluorimetric technique ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Tetrahymena pyriformis GL cells pretreated (imprinted) and not pretreated with insulin showed dissimilar quantitative relations of FITC-insulin binding. Displacement of FITC-insulin by unlabelled insulin was considerably less in the control than in the imprinted series. The curve for saturation of the binding sites with FITC-insulin resembled a true saturation curve. The imprinted cells bound considerably more hormone in a shorter time than the control cells at identical levels of exposure. The dissociation of bound hormone from the imprinted cells increased over the control at 23°C, and to a still greater degree at 4°C. The effect of the pH of the medium on the dissociation of bound FITC-insulin also differed between the imprinted and not imprinted cells. Thus the proposed cytofluorimetric assay of binding kinetics demonstrated the actual conditions of receptor activity, and indicated that the induced insulin binding sites of Tetrahymena behaved similarly to ‘classical’ receptors.
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  • 72
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    Keywords: Ligand binding ; respiratory burst ; neutrophils ; cytoplasts ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: When polymorphonuclear leukocytes (neutrophils) and soluble or particulate matter interact, the cells produce superoxide anions (O2-) and hydrogen peroxide (H2O2). The chemotactic peptide formylmethionyl-leucylphenylalanine (FMLP) induced a very weak response in normal neutrophils. The cellular response was changed, however, as a result of in vitro aging of the cells, i.e. the magnitude of the response was increased following storage of the cells at 22°C for up to 120 min, in the absence of any stimulus, and before the addition of the peptide. When phorbol myristate acetate was used as a stimulus, there was a pronounced production of O2- and H2O2, but no change in magnitude as a result of in vitro aging. When neutrophil cytoplasts (granule-free vesicles of cytoplasm enclosed by plasmalemma) were exposed to the peptide FMLP of PMA, the vesicles produced both O2- and H2O2. There was, however, no increase in oxidative metabolite production in cytoplasts as a result of in vitro aging when either FMLP or PMA was used as a stimulus. The results thus indidate that mere incubation at room temperature primed the cells to increase their production of oxidative metabolites as a result of spontaneous exposure of hidden receptors. The fact that no such effects were observed with cytoplasts indicates that spontaneous receptor recruitment is a granule-dependent process.
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  • 73
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    Cell Biochemistry and Function 2 (1984), S. 38-42 
    ISSN: 0263-6484
    Keywords: Cytology ; ascites cells ; endoplasmic reticulum ; subfraction ; cytochalasin B ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Information on the interaction between endoplasmic reticulum (ER) membranes and components of the skeletal network of the cell was gained by treating cells with the antimicrofilament agent cytochalasin B prior to cell disruption by nitrogen cavitation. Treatment of Krebs II ascites cells with cytochalasin B (5-10 μg ml-1) resulted in an increased yield of three ER membrane subfractions  -  heavy rough (HR), light rough (LR) and smooth (S) membranes, as judged by 3H-choline incorporation in gradient fractions following discontinuous sucrose gradient centrifugation. The major increase was observed in the HR fraction. These results indicate that the actual yield of the respective ER membrane subfractions after cell disruption is dependent on the degree of direct and/or indirect interaction between individual ER membranes and actin containing filaments of the cytoskeleton in the intact cell.
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  • 74
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    Cell Biochemistry and Function 2 (1984), S. 43-48 
    ISSN: 0263-6484
    Keywords: Dental cement ; cytotoxicity ; in vitro ; glass ionomer cements ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The new glass ionomer dental cement, ChemFil, was found to be moderately cytotoxic when freshly mixed in vitro. This was assessed by a reduction in fibroblast and macrophage counts following 24 h exposure to the material, and by alterations in enzyme levels and enzyme staining kinetics relative to control cultures. Testing of set samples of the material showed that its toxicity decreased rapidly with setting. ChemFil behaved in a manner similar to two older glass ionomers, ASPA and ChemBond, although it was generally slightly less irritant.
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  • 75
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    Cell Biochemistry and Function 2 (1984), S. 53-56 
    ISSN: 0263-6484
    Keywords: Heart ; heparin ; lipoprotein lipase ; secretin ; cardiocytes ; sulphated glycosaminoglycans ; dexamethasone ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: When cardiac muscle cells from mature rats were incubated in vitro in the presence of heparin (8.7 nmole ml-1) lipoprotein lipase activity appeared in the incubation medium. The intracellular activity of the enzyme remained unchanged. Other glycosaminoglycans (heparan sulphate, dermatan sulphate, keratan sulphate and chrondroitin 6-sulphate) at the same or higher concentrations were totally ineffective in producing any enzyme redistribution between cells and medium. The release seen in the presence of heparin was blocked by the presence of cycloheximide. Cycloheximide by contrast had no effect on the release observed in the presence of dexamethasone, The action of endogenous glycosaminoglycans are unlikely therefore to have a significant role to play in the movement of lipoprotein lipase in heart tissue in vivo.
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  • 76
    ISSN: 0263-6484
    Keywords: Liver ; mitochondria ; enzyme activity ; ATPase ; respiratory control coefficient ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Rat liver mitochondria, stored with the energy-linked functions preserved or in aging conditions, were used to assay the activity of various enzymes during five days. The preservation of energy-linked functions was monitored by the respiratory control coefficient. ATPase, cytochrome oxidase and NADH dehydrogenase showed increased activity when the energy-linked functions were preserved. In aging conditions, cytochrome oxidase, NADH dehydrogenase and ATPase showed decreased activity. The ATPase activity increased only when mitochondria were stored in the presence of inhibitors of the electron transport chain. The activity of NADH oxidase did not change, and succinate oxidase and succinate dehydrogenase showed a small decrease in their activity. The enzymes of the matrix, α-ketoglutarate dehydrogenase, malate dehydrogenase and aspartate aminotransferase showed little decrease in activity under either of the conditions of storage. The total protein content decreased slightly under both conditions of storage. These results show that the activity of the enzymes analysed was maintained at reasonable levels, when the energy-linked functions of isolated mitochondria were preserved.
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  • 77
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    Cell Biochemistry and Function 2 (1984), S. 63-63 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
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  • 78
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    Cell Biochemistry and Function 8 (1990) 
    ISSN: 0263-6484
    Keywords: Chemistry ; Biochemistry and Biotechnology
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  • 79
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    Cell Biochemistry and Function 2 (1984), S. 57-61 
    ISSN: 0263-6484
    Keywords: Heart ; myocardial biopsies ; cryostat sections ; interference microscope ; densitometric trace ; oedema ischaemic arrest ; reperfusion ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Oedema following periods of ischaemic arrest and subsequent reperfusion has been shown experimentally and clinically to affect the functional state of the heart. Tissue water content has been measured in myocardial sections by microscopic interferometry and densitometry, and the results correlated with those obtained by wet and dry weight analysis (r = 0.87; p 〈 0.001). Microscopic interferometry also revealed the distribution of the water in the tissue. Experimentally induced ischaemic arrest in isolated rat hearts resulted in predominantly intra-fibrillar oedema, whilst subsequent reperfusion resulted in interfibrillar oedema. Microscopic interferometry facilitates accurate measurement of water content in tissue samples as small as 2 mg wet weight and shows (as conventional wet/dry weight analysis cannot) the distribution of the water in the tissue.
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    Cell Biochemistry and Function 2 (1984), S. 63-63 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
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    Cell Biochemistry and Function 2 (1984), S. 67-67 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
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  • 82
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    Cell Biochemistry and Function 13 (1995), S. 135-140 
    ISSN: 0263-6484
    Keywords: Macrophage ; lipoproteins ; superoxide ; free radicals ; oxidation ; atherosclerosis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Superoxide production by macrophages and leukocytes may have an important role in atherogenesis. Whether lipoproteins modulate the superoxide production of these cells is not clear. Therefore, the effect of lipoproteins on the production of superoxide by rat peritoneal macrophages was tested. VLDL and LDL inhibited digitonin-stimulated superoxide production in a dose-dependent manner. Maximum inhibition was observed at 10 μg ml-1 of VLDL protein and 50 μg ml-1 of LDL protein respectively. In contrast, HDL (40 μg protein ml-1) enhanced digitoninstimulated superoxide production (by 47 per cent). Macrophage superoxide production induced by arachidonic acid was enhanced by both VLDL (130 per cent) and HDL (84 per cent), whereas LDL had no effect. The lipoproteins had no effect on macrophage superoxide stimulated by other agonists such as phorbol myristate 13-acetate, sodium fluoride or the calcium ionophore, A23187. The effect of lipoproteins was also tested on human polymorphonuclear leukocyte superoxide generation, stimulated by digitonin and PMA. Ten μg of VLDL, 50 μg of LDL and 50 μg of HDL proteins ml-1, inhibited digitonin-induced superoxide production by 50, 100 and 33 per cent respectively. Lipoproteins had no effect on PMA stimulated superoxide generation by human polymorphonuclear leukocytes. The stimulatory and inhibitory effects of lipoproteins on macrophage and neutrophil superoxide generation could be important in the understanding of oxidation-mediated development of atherosclerosis.
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  • 83
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    Cell Biochemistry and Function 8 (1990), S. i 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
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  • 84
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    Cell Biochemistry and Function 8 (1990), S. 141-145 
    ISSN: 0263-6484
    Keywords: Cathepsin D ; postnatal changes ; liver ; brain ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Total and specific activity of cathepsin D (EC. 3.4.23.5) were measured in rat liver and brain from 1 to 98 days of age.The activity of cathepsin D in the liver of adult and newborn rats was the same while in the rat brain it was higher in adult than in newborn rats. In the liver maximum specific activity of cathepsin D occurred on the 10th postnatal day and minimum on the fourth day of age. In the brain maximum specific activity of the enzyme occurred on the 14th postnatal day. Total activity of cathepsin D increased after birth in rat liver and brain. These results are discussed in relation to the functional role of cathepsin D in the rat liver and the brain.
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  • 85
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    Keywords: 4-Hydroxynonenal ; chemiluminescence ; zymosan ; neutrophils ; respiratory burst ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The lipid peroxidation product 4-hydroxy-2,3-trans-nonenal (HNE) has a spectrum of biological effects on different cell types depending on the concentrations tested. In particular micromolar HNE concentrations stimulate neutrophil migration and polarization whereas higher doses inhibit.In our experimental conditions, fMet-Leu-Phe (fMLP) increased CL production of both unstimulated and zymosan-stimulated neutrophils, whereas cell stimulation with low HNE concentrations as well as zymosan addition to HNE incubated cells did not enhance light emission. In contrast 10-4 M HNE reduced CL emission by unstimulated cells nearly to background values, completely depressed CL production by zymosan-stimulated cells and reduced phagocytosis. Cysteine was found to be able to counteract the HNE effect by about 70 per cent. The possibility that this aldehyde could exert its inhibitory effect through the alkylation of NADPH-oxidase SH-groups is postulated. Moreover, our present data on differences observed between fMLP and HNE indicate a different chemotactic mechanism induced by these two classes of compounds and lead to the conclusion that the local functional features of the attracted cells may be different.
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  • 86
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    Keywords: Neutrophils ; superoxide ; myocardial infarction ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Circulating neutrophils isolated from patients 3-4 h after a myocardial infarction produced less \documentclass{article}\pagestyle{empty}\begin{document}$ {\rm O}\frac{ \cdot }{{\rm 2}} $\end{document} compared with controls, when stimulated with phorbol myrystate acetate or formyl-methionine-leucine-phenylalanine. Three days after the infraction the \documentclass{article}\pagestyle{empty}\begin{document}$ {\rm O}\frac{ \cdot }{{\rm 2}} $\end{document} generation elicited by both stimuli further decreased markedly. Seven and 15 days after infarction the \documentclass{article}\pagestyle{empty}\begin{document}$ {\rm O}\frac{ \cdot }{{\rm 2}} $\end{document} stimulated production was only slightly lower than or similar to the control values. The neutrophils of infarcted patients showed an augmented latency period before \documentclass{article}\pagestyle{empty}\begin{document}$ {\rm O}\frac{ \cdot }{{\rm 2}} $\end{document} production compared with controls in response to exogenous stimuli, particularly three days after infarction. Electron microscopy revealed that the neutrophils isolated from the infarcted patients displayed signs of cell exhaustion with few alterations of the plasma membranes when stimulated with phorbol ester. In contrast, control neutrophils displayed alterations of the plasma membranes characteristic of active neutrophils. The results of this study indicate that the circulating neutrophils appear exhausted and functionally inhibited immediately after myocardial infarction.
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    Cell Biochemistry and Function 8 (1990), S. 163-166 
    ISSN: 0263-6484
    Keywords: Mammary epithelial cell ; phosphatidylinositol ; phospholipase C ; insulin ; prolactin ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have previously demonstrated in vitro that, in the endoplasmic reticulum and Golgi apparatus of mammary epithelial cells of lactating and pregnant mice, inositol 1,4,5-trisphosphate releases Ca2+ that has been stored in these organelles. In this study, we examined whether insulin and prolactin, essential for the growth of mammary gland and for lactation, influenced the activity of phosphatidylinositol-specific phospholipase C in mammary cells. In the plasma membrane fraction of mammary epithelial cells of the DDY mouse strain 5 days after the start of lactation after the first pregnancy, and with phosphatidylinostol as substrate, it was shown that the activity of phospholipase C was enhanced by about four times in the presence of insulin compared with the control. Such enhancement was not found in the membrane fraction treated with prolactin.
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    Cell Biochemistry and Function 2 (1984), S. 140-144 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
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    Cell Biochemistry and Function 2 (1984), S. 144-148 
    ISSN: 0263-6484
    Keywords: Chemistry ; Biochemistry and Biotechnology
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    Cell Biochemistry and Function 2 (1984), S. 149-152 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
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  • 91
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    Cell Biochemistry and Function 2 (1984), S. 153-154 
    ISSN: 0263-6484
    Keywords: Tissue biology ; immunofluorescent staining ; monoclonal antibodies ; S-phase cells ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This note describes an immunofluorescent staining method for cells in the S-phase which have been allowed to take up bromodeoxyuridine into their DNA in place of thymine. The technique involves the use of fluorescinated monoclonal antibodies against bromodeoxyuridine and allows rapid and accurate estimation of cells in the S-phase, the technique does not require interpretation by skilled technicians.
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    Cell Biochemistry and Function 2 (1984), S. 167-170 
    ISSN: 0263-6484
    Keywords: Blood ; avian thrombocytes ; platelet ; adenine nucleotides ; malonyldialdehyde ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Analysis of free nucleotide composition of both avian thrombocytes and pig platelets showed quantitative differences in the level of adenine nucleotides. 3H-adenine taken up by turkey thrombocytes was metabolized mainly to adenine nucleotides was not released after thrombin action. Thrombin liberated non-radioactive adenine nucleotides (18.2 ± 1.5 %, 20.6 ± 1.9%) of the total, probably localized in a storage pool. Malonyldialdehyhyde (MDA) production due to thrombin was observed in both platelets and thrombocytes.
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  • 93
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    Cell Biochemistry and Function 2 (1984), S. 161-166 
    ISSN: 0263-6484
    Keywords: Insulin ; pancreas ; pancreatic islets ; insulin release ; proinsulin conversion ; transglutaminase ; methylamine ; trimethylamine ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The metabolic and secretory effects of methylamine in rat pancreatic islets were investigated. Methylamine accumulated in islet cells, was incorporated into endogenous islet proteins, and inhibited the incorporation of [2,5-3H] histamine into either N,N-dimethylcasein or endogenous islet proteins. Methylamine (2 mM) did not affect the oxidation of glucose or endogenous nutrients or the intracellular pH in islet cells. Glucose did not affect the activity of transglutaminase in islet homogenates, the uptake of 14C-methylamine by intact islets or its incorporation into endogenous islet proteins. Methylamine inhibited insulin release evoked by glucose, other nutrient secretagogues, and non-nutrient insulinotropic agents such as L-arginine or gliclazide. The inhibitory effect of methylamine upon insulin release was diminished in the presence of cytochalasin B or at low extracellular pH. Methylamine retarded the conversion of proinsulin to insulin. Trimethylamine (0.7 mM) was more efficiently taken up by islet cells than methylamine (2.0 mM), and yet caused only a modest inhibition of insulin release. These findings suggest that methylamine interferes with a late step in the secretory sequence, possibly by inhibiting the access of secretory granules to their exocytotic site.
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  • 94
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    Keywords: Fats ; adipose tissue ; cold adaptation ; mitochondria ; creatine kinase ; peroxisomes ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A new technique for single-step subcellular fractionation of adipose tissue homogenates by analytical sucrose density gradient centrifugation in a vertical pocket reorientating rotor is described. The density gradient distributions of mitochondrial and peroxisomal marker enzymes in brown and white adipose tissue of control and cold exposed rats are compared. The equilibrium density of brown fat mitochondria was found to be significantly increased compared with white fat mitochondria. GDP binding activity was localized solely to the mitochondria in both control and cold-adapted brown adipose tissue. Brown and white fat mitochondria fractions were isolated by differential centrifugation and the specific activities of various enzymes in the homogenate and mitochondrial preparations determined. The specific activity of creatine kinase in brown adipose tissue was found to be ten-fold higher than in white fat and subcellular fractionation studies showed the activity to have an exclusively cytosolic distribution in both tissues. GDP binding activity and some of the mitochondrial enzymes showed, in brown adipose, a striking increase in total activity in cold adapted rats compared to control animals. For some enzyme activities there was a small increase when expressed per mg tissue or per mg mitochondrial protein. When expressed per mg DNA i.e. per cell, there was a reduced specific activity of the mitochondrial and peroxisomal enzymes in both brown and white adipose tissue on cold adaptation.
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    Cell Biochemistry and Function 2 (1984), S. 171-176 
    ISSN: 0263-6484
    Keywords: Blood ; human erythrocyte ; erythrocyte membrane ; choline phospholipid ; acyl chain ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Intact human erythrocytes were treated, under non-haemolytic conditions at 37°C, with synthetic phosphatidylcholine which has homologous, saturated acyl chains of 8 ∼ 18 even-numbered carbon atoms (C8 ∼ C18-PC) or with lysophosphatidylcholine which has a saturated acyl chain of 8 ∼ 18 carbon atoms (C8 ∼ C18-lysoPC). The C8 ∼ C14-PC and C12 ∼ C18-lysoPC species were rapidly incorporated into the erythrocytes and induced a shape change of the crenation (echinocyte formation) type. The site of the incorporation was found to be most probably on the outer leaflet of the membrane lipid bilayer. The extent of the shape change was dependent on the amount of each lipid incorporated. When the same amount of a PC or lysoPC species was incorporated into the membrane, about the same extent of crenation was induced, independent of acyl chain length. However, C16-PC, C18-PC, C8-lysoPC and C10-lysoPC, which were not incorporated into the erythrocytes, did not induce any shape change. It is therefore suggested that the hydrophobic moiety of these amphiphilic lipids may greatly contribute to their transfer from the outer medium into the erythrocyte membrane, but do not influence so much the perturbation of the membrane lipid bilayer which may be responsible for induction of the shape change.
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    Cell Biochemistry and Function 8 (1990), S. 190-190 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
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    Cell Biochemistry and Function 8 (1990) 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
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  • 98
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    Keywords: HeLa cells ; methotrexate ; anti-cancer ; malate-aspartate shuttle ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effect of methotrexate (MTX) on the mitochondrial oxidation of cytosolic-reducing equivalents in HeLa cells was studied. MTX inhibited (100 per cent) malate dehydrogenase activity, but no effect was observed on that of GOT. MTX (0.5 mM) inhibited (100 per cent) the activity of reconstituted enzymatic system MDH-GOT, probably as a consequence of inhibition of malate dehydrogenase activity. MTX decreased pyruvate production (54 per cent), demonstrating its inhibitory action on the malate-aspartate shuttle. Blockage of the malate-aspartate shuttle by MTX accounts for the decrease in cellular energetic gain. The results obtained are consistent with the view that in HeLa cells, as well as in other tumour cells, the trasport of reducing equivalents from cytoplasmic NADH into the respiratory chain of mitochondria is via the malate-aspartate shuttle.
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  • 99
    ISSN: 0263-6484
    Keywords: Inositol 1,4,5-trisphosphate ; Ca2+ ; mammary epithelial cell ; endoplasmic reticulum ; Golgi apparatus ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: It has been established that inositol 1,4,5-trisphosphate(IP3) is responsible for the mobilization of calcium(Ca2+) from intracellular locations in a wide variety of tissues, and that this response triggers the stimulation of several hormones and neurotransmitters. However, these phenomena have yet to be examined in the mammary epithelium.Ca2+ uptake from the medium into the endoplasmic reticulum(ER) and Golgi apparatus in vitro in both pregnant and lactating mouse mammary epithelial cells was studied and a strong Ca2+ release from these organelles into the medium with the use of IP3 was shown. The Ca2+ uptake and its release due to IP3 was also usually greater during pregnancy than lactation.
    Additional Material: 2 Ill.
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  • 100
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 8 (1990), S. 221-226 
    ISSN: 0263-6484
    Keywords: Rheumatoid arthritis ; chronic inflammatory arthritis ; glucose 6-phosphate dehydrogenase ; menadione ; microdensitometry ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The immunological induction of arthritis in the knee of the rabbit is well established as a model for human rheumatoid arthritis. It has the special advantage of allowing the development of the condition, and the effect of disease-modifying agents, to be followed.Attention has been focussed on the activity of glucose 6-phosphate dehydrogenase in the synovial lining cells since the fourfold elevation of this activity was shown to be fundamental in the human condition. An equal elevation of this activity has now been demonstrated in the rabbit model. Furthermore, it has been shown that the oral administration of menadione decreases this activity towards normality with a concomitant decrease in the degree of inflammation.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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