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  • Animals  (3.947)
  • Nature Publishing Group (NPG)  (3.947)
  • 2010-2014  (3.947)
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  • 1
    Publikationsdatum: 2010-11-19
    Beschreibung: Cellular messenger RNA (mRNA) of higher eukaryotes and many viral RNAs are methylated at the N-7 and 2'-O positions of the 5' guanosine cap by specific nuclear and cytoplasmic methyltransferases (MTases), respectively. Whereas N-7 methylation is essential for RNA translation and stability, the function of 2'-O methylation has remained uncertain since its discovery 35 years ago. Here we show that a West Nile virus (WNV) mutant (E218A) that lacks 2'-O MTase activity was attenuated in wild-type primary cells and mice but was pathogenic in the absence of type I interferon (IFN) signalling. 2'-O methylation of viral RNA did not affect IFN induction in WNV-infected fibroblasts but instead modulated the antiviral effects of IFN-induced proteins with tetratricopeptide repeats (IFIT), which are interferon-stimulated genes (ISGs) implicated in regulation of protein translation. Poxvirus and coronavirus mutants that lacked 2'-O MTase activity similarly showed enhanced sensitivity to the antiviral actions of IFN and, specifically, IFIT proteins. Our results demonstrate that the 2'-O methylation of the 5' cap of viral RNA functions to subvert innate host antiviral responses through escape of IFIT-mediated suppression, and suggest an evolutionary explanation for 2'-O methylation of cellular mRNA: to distinguish self from non-self RNA. Differential methylation of cytoplasmic RNA probably serves as an example for pattern recognition and restriction of propagation of foreign viral RNA in host cells.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058805/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058805/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Daffis, Stephane -- Szretter, Kristy J -- Schriewer, Jill -- Li, Jianqing -- Youn, Soonjeon -- Errett, John -- Lin, Tsai-Yu -- Schneller, Stewart -- Zust, Roland -- Dong, Hongping -- Thiel, Volker -- Sen, Ganes C -- Fensterl, Volker -- Klimstra, William B -- Pierson, Theodore C -- Buller, R Mark -- Gale, Michael Jr -- Shi, Pei-Yong -- Diamond, Michael S -- R01 AI074973/AI/NIAID NIH HHS/ -- R01 AI56540/AI/NIAID NIH HHS/ -- R01 CA068782/CA/NCI NIH HHS/ -- R01 CA068782-24/CA/NCI NIH HHS/ -- U19 AI083019/AI/NIAID NIH HHS/ -- U54 AI057158/AI/NIAID NIH HHS/ -- U54 AI057160/AI/NIAID NIH HHS/ -- U54 AI057160-06/AI/NIAID NIH HHS/ -- U54 AI081680/AI/NIAID NIH HHS/ -- U54 AI081680-01/AI/NIAID NIH HHS/ -- England -- Nature. 2010 Nov 18;468(7322):452-6. doi: 10.1038/nature09489.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21085181" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): 3T3 Cells ; Animals ; Carrier Proteins/genetics/*metabolism ; Cells, Cultured ; Coronavirus/enzymology/genetics/immunology/physiology ; Fibroblasts ; Gene Expression Regulation/genetics/*immunology ; Humans ; Immunity, Innate/genetics/*immunology ; Interferons/deficiency/genetics/*immunology ; Methylation ; Methyltransferases/metabolism ; Mice ; Mice, Inbred C57BL ; Models, Genetic ; Models, Immunological ; Neoplasm Proteins/genetics/metabolism ; Poxviridae/enzymology/genetics/immunology/physiology ; Protein Biosynthesis/immunology ; Proteins/genetics/*metabolism ; RNA Caps/genetics/immunology/*metabolism ; RNA, Viral/genetics/immunology/*metabolism ; Receptor, Interferon alpha-beta/deficiency/genetics ; Survival Rate ; Virus Replication ; West Nile virus/enzymology/genetics/immunology/physiology
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 2
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2010-01-08
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉England -- Nature. 2010 Jan 7;463(7277):26-32. doi: 10.1038/463026a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20054379" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Age Distribution ; Agriculture/methods/trends ; Animals ; Astronomy/trends ; Bioengineering/methods/trends ; Chemistry/trends ; Conservation of Energy Resources/economics/trends ; Demography ; Drug Industry/trends ; Ecology/trends ; Green Chemistry Technology/trends ; Hominidae ; Humans ; International Cooperation ; Internet/trends ; Lasers/utilization ; Mental Disorders/genetics/therapy ; Metabolomics/trends ; Metagenome ; National Institutes of Health (U.S.)/organization & administration/trends ; Paleontology/trends ; Precision Medicine/ethics/trends ; Research/education/*trends ; Search Engine/methods/trends ; Soil/standards ; United States ; Universities/standards/trends
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 3
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    Nature Publishing Group (NPG)
    Publikationsdatum: 2011-12-24
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Van Noorden, Richard -- England -- Nature. 2011 Dec 21;480(7378):426-9. doi: 10.1038/480426a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22193080" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Disasters ; Drug Industry/economics ; Humans ; Physics/trends ; Science/economics/*trends ; Scientific Misconduct ; Sequence Analysis, DNA ; Stem Cells
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 4
    Publikationsdatum: 2013-12-20
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Van Noorden, Richard -- Tollefson, Jeff -- Hayden, Erika Check -- Morello, Lauren -- Shen, Helen -- Butler, Declan -- Ledford, Heidi -- Witze, Alexandra -- Reich, Eugenie Samuel -- Schiermeier, Quirin -- Cyranoski, David -- England -- Nature. 2013 Dec 19;504(7480):344-9. doi: 10.1038/504344a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24352274" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Astronomy/economics ; Atmosphere/chemistry ; Coronavirus Infections/epidemiology ; Disease Eradication ; Federal Government ; Fossils ; Genes ; HIV Infections/drug therapy ; History, 21st Century ; Hominidae/classification/genetics ; Humans ; Influenza A Virus, H7N9 Subtype/pathogenicity ; Neurosciences/economics/trends ; Patents as Topic/legislation & jurisprudence ; Poliomyelitis/epidemiology ; *Science/economics/history ; Stem Cell Research ; United States
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 5
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    Unbekannt
    Nature Publishing Group (NPG)
    Publikationsdatum: 2012-12-22
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Van Noorden, Richard -- England -- Nature. 2012 Dec 20;492(7429):324-7. doi: 10.1038/492324a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23257860" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Drug Approval ; Drug Industry/economics ; Economic Recession ; Encyclopedias as Topic ; Food, Genetically Modified/standards ; Genetics ; Global Warming ; History, 21st Century ; Humans ; Influenza A Virus, H5N1 Subtype/genetics/pathogenicity ; Mars ; Nuclear Energy ; Physics ; Science/*history ; Space Flight/instrumentation ; Transportation/legislation & jurisprudence
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 6
    Publikationsdatum: 2012-12-22
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Heuer, Rolf-Dieter -- Rosenzweig, Cynthia -- Steltzner, Adam -- Blanpain, Cedric -- Iorns, Elizabeth -- Wang, Jun -- Handelsman, Jo -- Gowers, Tim -- De Bernardinis, Bernardo -- Fouchier, Ron -- England -- Nature. 2012 Dec 20;492(7429):335-43. doi: 10.1038/492335a.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23257862" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Access to Information ; Animals ; Bioterrorism/prevention & control ; Climate Change ; Disaster Planning/history ; Earthquakes/statistics & numerical data ; Forecasting ; Genomics ; History, 20th Century ; History, 21st Century ; Humans ; Influenza A Virus, H5N1 Subtype/genetics/pathogenicity ; Influenza, Human/transmission/virology ; Mars ; Neoplastic Stem Cells/cytology ; New York City ; Physics/history ; Publishing/economics ; Reproducibility of Results ; *Research/economics/standards/statistics & numerical data ; Security Measures ; Sexism/psychology/statistics & numerical data ; Space Flight/history/instrumentation
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 7
    Publikationsdatum: 2013-06-14
    Beschreibung: 53BP1 (also called TP53BP1) is a chromatin-associated factor that promotes immunoglobulin class switching and DNA double-strand-break (DSB) repair by non-homologous end joining. To accomplish its function in DNA repair, 53BP1 accumulates at DSB sites downstream of the RNF168 ubiquitin ligase. How ubiquitin recruits 53BP1 to break sites remains unknown as its relocalization involves recognition of histone H4 Lys 20 (H4K20) methylation by its Tudor domain. Here we elucidate how vertebrate 53BP1 is recruited to the chromatin that flanks DSB sites. We show that 53BP1 recognizes mononucleosomes containing dimethylated H4K20 (H4K20me2) and H2A ubiquitinated on Lys 15 (H2AK15ub), the latter being a product of RNF168 action on chromatin. 53BP1 binds to nucleosomes minimally as a dimer using its previously characterized methyl-lysine-binding Tudor domain and a carboxy-terminal extension, termed the ubiquitination-dependent recruitment (UDR) motif, which interacts with the epitope formed by H2AK15ub and its surrounding residues on the H2A tail. 53BP1 is therefore a bivalent histone modification reader that recognizes a histone 'code' produced by DSB signalling.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955401/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955401/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fradet-Turcotte, Amelie -- Canny, Marella D -- Escribano-Diaz, Cristina -- Orthwein, Alexandre -- Leung, Charles C Y -- Huang, Hao -- Landry, Marie-Claude -- Kitevski-LeBlanc, Julianne -- Noordermeer, Sylvie M -- Sicheri, Frank -- Durocher, Daniel -- 84297-1/Canadian Institutes of Health Research/Canada -- 84297-2/Canadian Institutes of Health Research/Canada -- MOP84297/Canadian Institutes of Health Research/Canada -- England -- Nature. 2013 Jul 4;499(7456):50-4. doi: 10.1038/nature12318. Epub 2013 Jun 12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23760478" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Motifs ; Amino Acid Sequence ; Animals ; Cell Cycle Proteins/chemistry/metabolism ; Cell Line ; Chromosomal Proteins, Non-Histone/chemistry/deficiency/genetics ; DNA Breaks, Double-Stranded ; *DNA Damage ; DNA-Binding Proteins/chemistry/deficiency/genetics ; Female ; Histones/*chemistry/*metabolism ; Humans ; Intracellular Signaling Peptides and ; Proteins/chemistry/deficiency/genetics/*metabolism ; Lysine/*metabolism ; Male ; Mice ; Molecular Sequence Data ; Mutant Proteins/chemistry/metabolism ; Nuclear Proteins/chemistry/metabolism ; Nucleosomes/chemistry/metabolism ; Protein Binding ; Protein Structure, Tertiary ; Schizosaccharomyces ; Schizosaccharomyces pombe Proteins/chemistry/metabolism ; Signal Transduction ; Ubiquitin/*metabolism ; *Ubiquitination
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 8
    Publikationsdatum: 2011-05-27
    Beschreibung: Acquired immune deficiency syndrome (AIDS), malaria and tuberculosis collectively cause more than five million deaths per year, but have nonetheless eluded conventional vaccine development; for this reason they represent one of the major global public health challenges as we enter the second decade of the twenty-first century. Recent trials have provided evidence that it is possible to develop vaccines that can prevent infection by human immunodeficiency virus (HIV) and malaria. Furthermore, advances in vaccinology, including novel adjuvants, prime-boost regimes and strategies for intracellular antigen presentation, have led to progress in developing a vaccine against tuberculosis. Here we discuss these advances and suggest that new tools such as systems biology and structure-based antigen design will lead to a deeper understanding of mechanisms of protection which, in turn, will lead to rational vaccine development. We also argue that new and innovative approaches to clinical trials will accelerate the availability of these vaccines.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rappuoli, Rino -- Aderem, Alan -- England -- Nature. 2011 May 26;473(7348):463-9. doi: 10.1038/nature10124.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Novartis Vaccines and Diagnostics, 53100 Siena, Italy. rino.rappuoli@novartis.com〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21614073" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): *AIDS Vaccines/chemistry/immunology ; Animals ; Antigens/chemistry/immunology ; Clinical Trials as Topic ; HIV Infections/epidemiology/immunology/prevention & control ; Humans ; Malaria/epidemiology/immunology/parasitology/prevention & control ; *Malaria Vaccines/chemistry/immunology ; Systems Biology/trends ; Tuberculosis/epidemiology/immunology/microbiology/prevention & control ; *Tuberculosis Vaccines/chemistry/immunology
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 9
    Publikationsdatum: 2014-11-11
    Beschreibung: The incompleteness of the fossil record obscures the origin of many of the more derived clades of vertebrates. One such group is the Ichthyopterygia, a clade of obligatory marine reptiles that appeared in the Early Triassic epoch, without any known intermediates. Here we describe a basal ichthyosauriform from the upper Lower Triassic (about 248 million years ago) of China, whose primitive skeleton indicates possible amphibious habits. It is smaller than ichthyopterygians and had unusually large flippers that probably allowed limited terrestrial locomotion. It also retained characteristics of terrestrial diapsid reptiles, including a short snout and body trunk. Unlike more-derived ichthyosauriforms, it was probably a suction feeder. The new species supports the sister-group relationships between ichthyosauriforms and Hupehsuchia, the two forming the Ichthyosauromorpha. Basal ichthyosauromorphs are known exclusively from south China, suggesting that the clade originated in the region, which formed a warm and humid tropical archipelago in the Early Triassic. The oldest unequivocal record of a sauropterygian is also from the same stratigraphic unit of the region.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Motani, Ryosuke -- Jiang, Da-Yong -- Chen, Guan-Bao -- Tintori, Andrea -- Rieppel, Olivier -- Ji, Cheng -- Huang, Jian-Dong -- England -- Nature. 2015 Jan 22;517(7535):485-8. doi: 10.1038/nature13866. Epub 2014 Nov 5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Earth and Planetary Sciences, University of California, Davis, One Shields Avenue, Davis, California 95616, USA. ; 1] Laboratory of Orogenic Belt and Crustal Evolution, Ministry of Education, and Department of Geology and Geological Museum, Peking University, Yiheyuan Street 5, Beijing 100871, China [2] State Key Laboratory of Palaeobiology and Stratigraphy (Nanjing Institute of Geology and Palaeontology, Chinese Academy of Science), Nanjing 210008, China. ; Department of Research, Anhui Geological Museum, Jiahe Road 999, Hefei, Anhui 230031, China. ; Dipartimento di Scienze della Terra, Universita degli Studi di Milano, Via Mangiagalli, 34-20133 Milan, Italy. ; Center of Integrative Research, The Field Museum, Chicago, Illinois 60605-2496, USA. ; Key Laboratory of Economic Stratigraphy and Palaeogeography, Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences, 39 East Beijing Road, Nanjing 210008, China.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25383536" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animal Structures/*anatomy & histology ; Animals ; China ; Fossils ; *Phylogeny ; Reptiles/*anatomy & histology/*classification ; Skull/anatomy & histology
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    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 10
    Publikationsdatum: 2012-07-24
    Beschreibung: One defining characteristic of the mammalian brain is its neuronal diversity. For a given region, substructure, layer or even cell type, variability in neuronal morphology and connectivity persists. Although it is well known that such cellular properties vary considerably according to neuronal type, the substantial biophysical diversity of neurons of the same morphological class is typically averaged out and ignored. Here we show that the amplitude of hyperpolarization-evoked sag of membrane potential recorded in olfactory bulb mitral cells is an emergent, homotypic property of local networks and sensory information processing. Simultaneous whole-cell recordings from pairs of cells show that the amount of hyperpolarization-evoked sag potential and current (Ih) is stereotypic for mitral cells belonging to the same glomerular circuit. This is corroborated by a mosaic, glomerulus-based pattern of expression of the HCN2 (hyperpolarization-activated cyclic nucleotide-gated channel 2) subunit of the Ih channel. Furthermore, inter-glomerular differences in both membrane potential sag and HCN2 protein are diminished when sensory input to glomeruli is genetically and globally altered so that only one type of odorant receptor is universally expressed. Population diversity in this intrinsic property therefore reflects differential expression between local mitral cell networks processing distinct odour-related information.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3442227/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3442227/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Angelo, Kamilla -- Rancz, Ede A -- Pimentel, Diogo -- Hundahl, Christian -- Hannibal, Jens -- Fleischmann, Alexander -- Pichler, Bruno -- Margrie, Troy W -- 085509/Wellcome Trust/United Kingdom -- MC_U117597156/Medical Research Council/United Kingdom -- MC_U1175975156/Medical Research Council/United Kingdom -- U.9500(97156)/Medical Research Council/United Kingdom -- Wellcome Trust/United Kingdom -- England -- Nature. 2012 Aug 16;488(7411):375-8. doi: 10.1038/nature11291.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, London WC1E 6BT, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22820253" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Female ; Gene Expression Profiling ; Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels ; Ion Channels/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Models, Neurological ; Nerve Net/*physiology ; Olfactory Bulb/*cytology/*physiology ; Potassium Channels ; Receptors, Odorant/metabolism ; Smell/*physiology
    Print ISSN: 0028-0836
    Digitale ISSN: 1476-4687
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
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