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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 5 (1992), S. 86-88 
    ISSN: 1432-2145
    Keywords: Sex chromosome markers ; Y-chromosome ; Angiosperms ; Silene latifolia ; Melandrium album
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to obtain markers for the Y chromosome ofSilene latifolia, we pooled equal weights of leaf tissue from 18 female siblings into one sample and repeated the process with 18 male siblings. Pooling was intended to provide a common genetic background for each sample, leaving the absence or presence of the Y chromosome as the primary difference between the two samples. DNA was extracted from each sample and subjected to polymerase chain reaction (PCR) amplification with arbitrary 10 bp primers. Four of 60 primers used gave an amplification with the male DNA not found among those from the female DNA. Each of these was subsequently shown to provide a reliable marker for the Y chromosome.
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  • 2
    ISSN: 1432-2242
    Keywords: DNA fingerprinting ; Repetitive DNA ; Genotype identification ; Angiosperms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Oligonucleotides hybridizing to simple repetitive DNA patterns are highly informative as probes for DNA fingerprinting in all investigated animal species, including man. Here we demonstrate the applicability of this technique in higher plants. The oligonucleotide probes (GTG)5 and (GATA)4 were used to investigate the differences in DNA fingerprint patterns of the following angiosperm species: Triticum aestivum, Secale cereale, Hordeum vulgare, Beta vulgaris, Petunia hybrida, Brassica oleracea, and Nicotiana tabacum. Two species, Hordeum vulgare as a monocot and Beta vulgaris as a dicot, were analyzed in more detail. Their genomes differ considerably in both amount and organization of the simple repetitive sequences (GATA)n, (GACA)n, (GTG)n, and (CT)n due to the evolutionary distance of these two species. Furthermore, several lines and cultivars of Beta vulgaris and Hordeum vulgare can clearly be distinguished on the basis of their highly polymorphic patterns of these repetitive sequences.
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  • 3
    ISSN: 1432-1432
    Keywords: Chloroplast 4.5S rRNA ; Cytosolic and chloroplast 5S rRNAs ; 5.8S rRNA ; 18S rRNA ; Nucleotide sequences ; Phylogenetic trees ; Angiosperms ; Gymnosperms ; Monocotyledons ; Dicotyledons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Complete or partial nucleotide sequences of five different rRNA species, coded by nuclear (18S, 5.8S, and 5S) or chloroplast genomes (5S, 4.5S) from a number of seed plants were determined. Based on the sequence data, the phylogenetic dendrograms were built by two methods, maximum parsimony and compatibility. The topologies of the trees for different rRNA species are not fully congruent, but they share some common features. It may be concluded that both gymnosperms and angiosperms are monophyletic groups. The data obtained suggest that the divergence of all the main groups of extant gymnosperms occurred after the branching off of the angiosperm lineage. As the time of divergence of at least some of these gymnosperm taxa is traceable back to the early Carboniferous, it may be concluded that the genealogical splitting of gymnosperm and angiosperm lineages occurred before this event, at least 360 million years ago, i.e., much earlier than the first angiosperm fossils were dated. Ancestral forms of angiosperms ought to be searched for among Progymnospermopsida. Genealogical relationships among gymnosperm taxa cannot be deduced unambiguously on the basis of rRNA data. The only inference may be that the taxon Gnetopsida is an artificial one, andGnetum andEphedra belong to quite different lineages of gymnosperms. As to the phylogenetic position of the two Angiospermae classes, extant monocotyledons seem to be a paraphyletic group located near the root of the angiosperm branch; it emerged at the earliest stages of angiosperm evolution. We may conclude that either monocotyledonous characters arose independently more than once in different groups of ancient Magnoliales or that monocotyledonous forms rather than dicotyledonous Magnoliales were the earliest angiosperms. Judging by the rRNA trees, Magnoliales are the most ancient group among dicotyledons. The most ancient lineage among monocotyledons leads to modern Liliaceae.
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  • 4
    Electronic Resource
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    Springer
    Machine vision and applications 8 (1995), S. 187-193 
    ISSN: 1432-1769
    Keywords: Tracking ; Segmentation ; Pigs ; Animals ; Computer vision
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract An algorithm was developed for the segmentation and tracking of piglets and tested on a 200-image sequence of 10 piglets moving on a straw background. The image-capture rate was 1 image/140 ms. The segmentation method was a combination of image differencing with respect to a median background and a Laplacian operator. The features tracked were blob edges in the segmented image. During tracking, the piglets were modelled as ellipses initialised on the blobs. Each piglet was tracked by searching for blob edges in an elliptical window about the piglet's position, which was predicted from its previous two positions.
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  • 5
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    Journal of molecular evolution 41 (1995), S. 238-246 
    ISSN: 1432-1432
    Keywords: Cellular slime molds ; Animals ; Fungi ; Plantae ; Maximum-likelihood method ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The phylogenetic position of Dictyostelium inferred from 18S rRNA data contradicts that from protein data. Protein trees always show the close affinity of Dictyostelium with animals, fungi, and plants, whereas in 18S rRNA trees the branching of Dictyostelium is placed at a position before the massive radiation of protist groups including the divergence of the three kingdoms. To settle this controversial issue and to determine the correct position of Dictyostelium, we inferred the phylogenetic relationship among Dictyostelium and the three kingdoms Animalia, Fungi, and Plantae by a maximum-likelihood method using 19 different protein data sets. It was shown at the significance level of 1 SE that the branching of Dictyostelium antedates the divergence of Animalia and Fungi, and Plantae is an outgroup of the Animalia-Fungi-Dictyostelium clade.
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  • 6
    ISSN: 1432-1432
    Keywords: CpG suppression ; GC content ; Angiosperms ; Isochores ; GC bias ; Mutational pressure ; Error-prone repair ; Transcriptionally coupled repair
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nuclear protein coding sequences from gymnosperms are currently scarce. We have determined 4 kb of nuclear protein coding sequences from gymnosperms and have collected and analyzed 〉60 kb of nuclear sequences from gymnosperms and nonspermatophytes in order to better understand processes influencing genome evolution in plants. We show that conifers possess both biased and nonbiased genes with respect to GC content, as found in monocots, suggesting that the common ancestor of conifers and monocots may have possessed both biased and nonbiased genes. The lack of biased genes in dicots is suggested to be a derived character for this lineage. We present a simple but speculative model of land-plant genome evolution which considers changes in GC bias and CpG frequency, respectively, as independent processes and which can account for several puzzling aspects of observed nucleotide frequencies in plant genes.
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  • 7
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    Journal of molecular evolution 42 (1996), S. 183-193 
    ISSN: 1432-1432
    Keywords: Small-subunit ribosomal RNA ; Phylogeny ; Animals ; Fungi ; Plants ; Alveolates ; Heterokonts ; Stramenopiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The evolutionary relationships of four eukaryotic kingdoms—Animalia, Plantae, Fungi, and Protista—remain unclear. In particular, statistical support for the closeness of animals to fungi rather than to plants is lacking, and a preferred branching order of these and other eukaryotic lineages is still controversial even though molecular sequences from diverse eukaryotic taxa have been analyzed. We report a statistical analysis of 214 sequences of nuclear small-subunit ribosomal RNA (srRNA) gene undertaken to clarify these evolutionary relationships. We have considered the variability of substitution rates and the nonindependence of nucleotide substitution across sites in the srRNA gene in testing alternative hypotheses regarding the branching patterns of eukaryote phylogeny. We find that the rates of evolution among sites in the srRNA sequences vary substantially and are approximately gamma distributed with size and shape parameter equal to 0.76. Our results suggest that (1) the animals and true fungi are indeed closer to each other than to any other “crown” group in the eukaryote tree, (2) red algae are the closest relatives of animals, true fungi, and green plants, and (3) the heterokonts and alveolates probably evolved prior to the divergence of red algae and animal-fungus-green-plant lineages. Furthermore, our analyses indicate that the branching order of the eukaryotic lineages that diverged prior to the evolution of alveolates may be generally difficult to resolve with the srRNA sequence data.
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  • 8
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    Journal of molecular evolution 43 (1996), S. 399-404 
    ISSN: 1432-1432
    Keywords: Asarum ; Dioscorea ; Angiosperms ; Evolution ; Legumins ; Seed proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analysis of legumin-encoding cDNAs fromDioscorea caucasica Lipsky (Dioscoreaceae) and fromAsarum europaeum L. (Aristolochiaceae) shows that there is an especially methionine-rich legumin subfamily present in the lower angiosperm clades including the Monocotyledoneae. It is characterized by a methionine content of 3–4 mol% which is roughly triple the methionine proportion of most other legumins. These “MetR” legumins, if present, still have to be detected in the higher angiosperms including the important seed crops. Evolutionary analysis suggests that the MetR legumins are the result of a gene duplication allowing the differentiation of legumin genes according to their sulfur content. The duplication event must have taken place before the split into mono- and dicotyledonous plants but probably after the separation of angiosperms and gymnosperms.
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  • 9
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    Environmental management 8 (1984), S. 309-324 
    ISSN: 1432-1009
    Keywords: Animals ; Indicators ; Air pollution ; Ecosystem responses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract With existing and proposed air-quality regulations, ecological disasters resulting from air emissions such as those observed at Copperhill, Tennessee, and Sudbury, Ontario, are unlikely. Current air-quality standards, however, may not protect ecosystems from subacute and chronic exposure to air emissions. The encouragement of the use of coal for energy production and the development of the fossil-fuel industries, including oil shales, tar sands, and coal liquification, point to an increase and spread of fossil-fuel emissions and the potential to influence a number of natural ecosystems. This paper reviews the reported responses of ecosystems to air-borne pollutants and discusses the use of animals as indicators of ecosystem responses to these pollutants. Animal species and populations can act as important indicators of biotic and abiotic responses of aquatic and terrestrial ecosystems. These responses can indicate long-term trends in ecosystem health and productivity, chemical cycling, genetics, and regulation. For short-term trends, fish and wildlife also serve as monitors of changes in community structure, signaling food-web contamination, as well as providing a measure of ecosystem vitality. Information is presented to show not only the importance of animals as indicators of ecosystem responses to air-quality degradation, but also their value as air-pollution indices, that is, as air-quality-related values (AQRV), required in current air-pollution regulation.
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  • 10
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    Plant cell reports 18 (1998), S. 143-147 
    ISSN: 1432-203X
    Keywords: Key wordsNicotiana tabacum ; Male germ unit ; Scanning electron microscopy ; Sperm isolation ; Angiosperms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sperm cells are released from pollen tubes of tobacco as linked cells, associated with the vegetative nucleus in an assemblage known as the male germ unit (MGU). Using light microscopy, the MGU assemblage appears to be ensheathed by cytoplasmic material of the pollen tube, which may stabilize their association. Following their release, the shape of the sperm cells and vegetative nucleus changes from an ellipsoidal to a more spheroidal morphology. When most of the cytoplasmic material is dispersed, a boundary remains around the two sperm cells. Using scanning electron microscopy, the cytoplasmic material surrounding the MGU appears filamentous, sometimes twisted and rope-like. Based on these observations, the function of the MGU of tobacco is discussed.
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  • 11
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    Evolutionary ecology 5 (1991), S. 231-247 
    ISSN: 1573-8477
    Keywords: Angiosperms ; pollination ; seed size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Seed plants capture pollen before seeds are dispersed and abort unpollinated ovules. As a result, each seed is associated with an accessory cost that represents the costs of pollen capture and the costs of aborted ovules. Accessory costs may explain the minimum seed size among species, because these costs are likely to comprise a greater proportion of total reproductive allocation in species with smaller seeds. For very small propagules, the costs of pollination may not be worth the benefits, perhaps explaining the persistence of pteridophytic reproduction at small propagule sizes. The smallest angiosperm seeds are much smaller than the smallest gymnosperm seeds, both in the fossil record and in the modern flora. This suggests that angiosperms can produce pollinated ovules more cheaply than gymnosperms. Pollination becomes less efficient as a species decreases in abundance, and this loss of efficiency is greater for species with a higher accessory cost per seed. We propose that the greater reproductive efficiency of angiosperms when rare can explain why angiosperm-dominated floras were more speciose than the gymnosperm-dominated floras they replaced.
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  • 12
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    Journal of agricultural and environmental ethics 10 (1997), S. 249-267 
    ISSN: 1573-322X
    Keywords: Animals ; Asia ; consciousness ; Australia ; Hong Kong ; India ; Israel ; Japan ; New Zealand ; The Philippines ; Russia ; Singapore ; Thailand
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Philosophy
    Notes: Abstract The interactions between humans, animals and the environment have shaped human values and ethics, not only the genes that we are made of. The animal rights movement challenges human beings to reconsider interactions between humans and other animals, and maybe connected to the environmental movement that begs us to recognize the fact that there are symbiotic relationships between humans and all other organisms. The first part of this paper looks at types of bioethics, the implications of autonomy and the value of being alive. Then the level of consciousness of these relationships are explored in survey results from Asia and the Pacific, especially in the 1993 International Bioethics Survey conducted in Australia, Hong Kong, India, Israel, Japan, New Zealand, The Philippines, Russia, Singapore and Thailand. Very few mentioned animal consciousness in the survey, but there were more biocentric comments in Australia and Japan; and more comments with the idea of harmony including humans in Thailand. Comparisons between questions and surveys will also be made, in an attempt to describe what people imagine animal consciousness to be, and whether this relates to human ethics of the relationships.
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  • 13
    ISSN: 1615-6102
    Keywords: Angiosperms ; Cell cycle ; Double fertilization ; Endosperm ; Ephedra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fertilization inEphedra trifurca was examined with a combination of light and fluorescence microscopy. Developmental analysis clearly indicates that double fertilization events, similar to those described inE. nevadensis, regularly occur during the process of sexual reproduction inE. trifurca. In addition to the typical fusion of a sperm nucleus and egg nucleus, a second fertilization event occurs between the second sperm nucleus from an individual pollen tube and the ventral canal nucleus. Both of the fertilization events take place within the confines of an individual egg cell of the female gametophyte. Microspectrofluorometric data demonstrate that each nucleus involved in a sexual fusion event proceeds through the synthesis phase of the cell cycle and increases its DNA content from 1C to 2C before the process of nuclear fusion is completed. Photometric data also confirm that the product of the second fertilization event is equal in DNA content (4C) to the zygotic nucleus derived from the first fertilization event, and is prepared to enter into mitosis as a fully functional diploid nucleus.
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  • 14
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    Pharmacy world & science 13 (1991), S. 70-73 
    ISSN: 1573-739X
    Keywords: Algae ; Angiosperms ; Arthropods ; Bacteria ; Fungi ; Lichens ; Marine animals ; Quinones, naturally occurring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Angiosperms, fungi (including lichens), and bacteria are the main sources of natural quinones. Small numbers are present in algae, ferns, conifers, sponges, echinoderms, other marine animals, and arthropods. In angiosperms quinones have some chemotaxonomic value at the genus and family level but more surveys are required.
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  • 15
    ISSN: 1432-0878
    Keywords: Hypophysis ; Rostral pars distalis ; Mugil platanus ; Animals ; Prolactin hormone secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The rostral pars distalis (RPD) of the teleost Mugil platanus from animals pretreated with reserpine or 6-hydroxydopamine (6-HODA) were assayed for dopamine (DA) or noradrenaline (NA) or for prolactin hormone. Such determinations were coupled with electron microscopy. It was found that reserpine and 6-HODA produced a significant decrease in the content of DA, NA, and prolactin. Electron microscope studies revealed that prolactin cells became activated as judged by ultrastructural criteria. After 6-HODA treatment type “B” neurosecretory fibers entering the RPD became selectively destroyed. These observations lead us to suggest that prolactin secretion is under inhibitory control by type “B” neurosecretory fibers of adrenergic nature.
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  • 16
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    Plant systematics and evolution 123 (1975), S. 107-115 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Centaurea ; Chromosome numbers ; Flora of the Mediterranean and C. Europe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chromosome numbers of several Mediterranean and Central European species from the genusCentaurea L. have been studied. The material was collected in natural habitats, exceptC. thracica andC. maxima. The numbers forC. cineraria (2n = 36; tetraploid!),C. subtilis (2n = 22),C. sonchifolia (2n = 44),C. sphaerocephala (2n = 44),C. napifolia (2n = 22),C. achaia (2n = 22),C. thracica (2n = 18), andC. maxima (2n = 28) are reported for the first time. For the following species earlier counts are confirmed:C. cineraria (diploid, 2n = 18),C. maculosa (2n = 18),C. rhenana (2n = 18),C. triumfettii (2n = 22 + 0−1 B; first report of accessory chromosome for this species),C. scabiosa (2n = 20 + 0−3 B),C. crassifolia (2n = 30). B-chromosomes are reported for 3 populations ofCentaurea scabiosa from Berlin; they lack in 2 populations from Bayern and Hessen.
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  • 17
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    Plant systematics and evolution 134 (1980), S. 11-21 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Daucus ; Petroselinum ; Acer ; rRNA ; transcription ; precursors of less than 2.5 × 106 daltons ; processing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Actively dividing cells from parsley (Petroselinum crispum) and carrot (Daucus carota) (bothApiaceae) andAcer pseudoplatanus (Aceraceae) were used to detect the primary gene product of the rDNA in plant cells. Parsley and carrot cells were labelled with [32P] orthophosphate. In both cases only one high molecular weight rRNA precursor was present on polyacrylamide gels under non-denaturing conditions. Its molecular weight did not exceed 2.5 × 106 daltons. The component emerged from the heterogenous material after a labelling period of 5–10 min. In parsley cells 45 min after onset of incubation labelled mature rRNA (25S and 18S) arrived in the cytoplasm. InAcer pseudoplatanus (incubation period 60 min) two rapidly labelled components did emerge from polyacrylamide gels; their molecular weights were 2.3 and 3.2− 3.4 × 106 daltons. After electrophoresis under denaturing conditions the larger component was no longer present, thus indicating that it was an aggregate of different RNA molecules. The molecular weights of the rRNA precursors ofD. carota andP. crispum determined after electrophoresis in formamide gels were about 2.1 × 106 daltons. From these results we have no evidence for the existence of rRNA precursors exceeding the molecular weight of 2.5 × 106 daltons.
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  • 18
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    Plant systematics and evolution 134 (1980), S. 127-131 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Labiatae =Lamiaceae ; Eremostachys codonocalyx ; E. stenocalycina ; E. salangensis ; E. freitagii ; E. andersii ; new species ; Flora of Afghanistan and Iran
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Five new species are described:Eremostachys codonocalyx from NE. Iran (related toE. subspicata),E. stenocalycina from NE. Afghanistan (related toE. alberti andE. hissarica),E. salangensis from NE. Afghanistan (close toE. bamianica),E. freitagii from E. Afghanistan (similar but not closely related toE. vulnerans), andE. andersii from E. Afghanistan (similar but not closely related toE. vulnerans), andE. andersii from NE. Afghanistan of isolated systematic position.
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  • 19
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    Plant systematics and evolution 200 (1996), S. 125-140 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Colour change ; floral colour phase ; innate flower detection ; colour preference ; pollination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Many angiosperms have arranged their flowers in inflorescences forming a distinct signalling unit to flower visitors. In some species, the flowers of inflorescences undergo a temporal colour change corresponding exactly to a change in the reward status. Based on information obtained from the spectral reflection curves of pre-change and postchage colours of flower corollas and/or floral guides, it was possible to demonstrate that the colour phase associated with reward closely corresponds to the visual stimuli which trigger behavioural responses of inexperienced flower visitors, and that the colour phase associated with less reward corresponds to visual stimuli less attractive to naïve flower visitors. Reciprocal colour changes were not observed. It is to be assumed that the unidirectionality of floral colour changes is an adaptation of angiosperms aimed at the guidance of first-time flower visitors. Signalling reward to inexperienced flower visitors is an additional function of floral colour changes. The main function of floral colour changes, however, is to provide cues with which the flower visitors can learn to associate one colour phase with reward.
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  • 20
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    Plant systematics and evolution 137 (1981), S. 23-38 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rosaceae ; Uredinales ; Chromosome numbers ; rust susceptibility ; tribal classification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract BothNeuradoideae andChrysobalanoideae seem rust-free. TheSpiraeoideae andPomoideae are heavily susceptible toGymnosporangium rusts. While thePrunoideae resemble theRosoideae in being vulnerable to attacks ofPuccinia species, they are additionally susceptible toTranzschelia andThekopsora, whereas theRosoideae are characteristically afflicted byPhragmidium.—It is suggested (a) to treat theChrysobalanoideae as a separate family (Chrysobalanaceae), (b) to transferDryas from theRosoideae-Potentilleae-Dryadinae to theRosoideae-Cercocarpeae, and (c) to divide theRosoideae into two main groups of tribes: (i) the rust-freeKerrieae andCercocarpeae with x = 9, and (ii) the rust-susceptiblePotentilleae, Ulmarieae, Roseae andSanguisorbeae with x = 7.
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