Publication Date:
2010-07-27
Description:
Electrical gradients are critical for many biological processes, including the normal function of excitable tissues, left-right patterning, organogenesis and wound healing. The fundamental mechanisms that regulate the establishment and maintenance of such electrical polarities are poorly understood. Here we identify a gradient of electrical coupling across the developing ventricular myocardium using high-speed optical mapping of transmembrane potentials and calcium concentrations in the zebrafish heart. We excluded a role for differences in cellular excitability, connexin localization, tissue geometry and mechanical inputs, but in contrast we were able to demonstrate that non-canonical Wnt11 signals are required for the genesis of this myocardial electrical gradient. Although the traditional planar cell polarity pathway is not involved, we obtained evidence that Wnt11 acts to set up this gradient of electrical coupling through effects on transmembrane Ca(2+) conductance mediated by the L-type calcium channel. These data reveal a previously unrecognized role for Wnt/Ca(2+) signalling in establishing an electrical gradient in the plane of the developing cardiac epithelium through modulation of ion-channel function. The regulation of cellular coupling through such mechanisms may be a general property of non-canonical Wnt signals.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2921013/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2921013/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Panakova, Daniela -- Werdich, Andreas A -- Macrae, Calum A -- K08 HL068711/HL/NHLBI NIH HHS/ -- R21 GM075946/GM/NIGMS NIH HHS/ -- R21 GM075946-01/GM/NIGMS NIH HHS/ -- R21 GM075946-02/GM/NIGMS NIH HHS/ -- R21 GM075946-03/GM/NIGMS NIH HHS/ -- R21 GM075946-04/GM/NIGMS NIH HHS/ -- R21 HL098938/HL/NHLBI NIH HHS/ -- R21 HL098938-01/HL/NHLBI NIH HHS/ -- England -- Nature. 2010 Aug 12;466(7308):874-8. doi: 10.1038/nature09249. Epub 2010 Jul 25.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Brigham and Women's Hospital/Harvard Medical School, Cardiovascular Division, 75 Francis Street, Thorn 11, Boston, Massachusetts 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20657579" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Calcium/metabolism
;
Calcium Channels, L-Type/*metabolism
;
Calcium Signaling
;
*Electric Conductivity
;
Heart/embryology
;
Ion Channel Gating/*physiology
;
Myocardium/cytology/*metabolism
;
Myocytes, Cardiac/metabolism
;
*Signal Transduction
;
Wnt Proteins/deficiency/genetics/*metabolism
;
Zebrafish/*embryology/metabolism
;
Zebrafish Proteins/deficiency/genetics/*metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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