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  • Male  (1,821)
  • Column liquid chromatography  (1,216)
  • Nature Publishing Group (NPG)  (1,816)
  • Springer  (1,221)
  • 1
    Electronic Resource
    Electronic Resource
    Lack of association between free testosterone and bone density separate from age in elderly males (1993)
    Springer
    Calcified tissue international 52 (1993), S. 67-69 
    Details
    ISSN: 1432-0827
    Keywords: Male ; Osteoporosis ; Free testosterone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary It is unclear what proportion of the variance in bone density in elderly males is accounted for by testosterone status. We studied 112 ambulatory, elderly volunteers (mean age 71.7 years) and determined free testosterone (FT), as well as bone density measurements by photon absorptiometry at multiple sites. Our studies of 35 of these subjects 4 years later includedmorning FT and dual energy X-ray absorptiometry. There were no significant correlations between FT and bone density at multiple scanning sites with the effects of age partialed out. We suspect that our inability to detect a significant effect of FT on bone density was related to the relative strength of other determinants of bone density, as well as to the fact that FT values are far more dynamic than bone density.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00675629
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  • 2
    Electronic Resource
    Electronic Resource
    Sterols of male and female compound inflorescences of Zea mays L. (1976)
    Springer
    Planta 133 (1976), S. 89-93 
    Details
    ISSN: 1432-2048
    Keywords: Zea mays ; Inflorescences ; Male ; Female ; Sterols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A comparison has been made between the sterols of male and female inflorescences and of pollen from Zea mays. The female inflorescence was shown to contain cholesterol, 24-methylcholesterol, 24-ethyl-5,22-cholestadien-3β-ol, 24-ethylcholesterol and (28Z)-24-ethylidenecholesterol. Themale inflorescence contained the same five compounds together with 24-methylenecholesterol. Pollen contained 24-methylenecholesterol as its main sterol together with lesser amounts of cholesterol, 24-ethylcholesterol, (28Z)-24-ethylidenecholesterol, 24-methylene-5α-cholest-7-en-3β-ol and 4α-methyl-24-methylene-5α-cholest-7-en-3β-ol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386011
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  • 3
    Electronic Resource
    Electronic Resource
    Sterols of male and female flowers of Cucumis sativus (1977)
    Springer
    Planta 134 (1977), S. 115-117 
    Details
    ISSN: 1432-2048
    Keywords: Female ; Flowers ; Male ; Meiosis ; Sterols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sterols of male and female flowers of Cucumis sativus L. were similar in composition. The principal compound was 24ξ-ethyl-5α-cholesta-7,22-dien-3β-ol. Five other 5α-Δ7 were detected: 24ξ-methyl-7-ene, 24ξ-ethyl-7-ene, 24-ethyl-7,24(28)Z-diene, 24ξ-ethyl-7,25-diene and 24ξ-ethyl-7,22,25-triene. Small amounts of Δ5 (cholesterol, 24ξ-methylcholesterol and 24ξ-ethylcholesterol) were detected. The possible significance of these sterols is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384959
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of anther differentiation in cytoplasmic male sterile maize using a specific isozyme system (esterase) (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 469-473 
    Details
    ISSN: 1432-2242
    Keywords: Maize ; Pollen anther ; Esterase ; Male ; sterility ; Restorer genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary During anther development, characterized in maize plants with N cytoplasm, certain esterase isozymes in non-microspore cells decrease in amount with anther age and new isozymes appear in the developing microspores. In anthers from male sterile plants with cms T or cms C cytoplasm, neither of these changes in esterase patterns occurred. In anthers from plants with cms S cytoplasm, the decrease in the esterases of non-microsporogenous cells was observed but not the appearance of microspore esterases. In lines carrying cms S cytoplasm and nuclear restorer genes, esterase changes during anther development were as in normal fertile anthers. These results are discussed with respect to the phenomenon of cytoplasmic male sterility in the different maize genotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263415
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  • 5
    Electronic Resource
    Electronic Resource
    The ultrastructure of the pars distalis of the hypophysis in males of Triturus cristatus carnifex Laur., treated with the antiandrogen cyproterone acetate (1973)
    Springer
    Cell & tissue research 137 (1973), S. 209-221 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Male ; Triturus cristatus carnifex Laur. ; “Globular basophil cells” ; Antiandrogen treatment ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adenohypophysis of the newt Triturus cristatus carnifex Laur. shows three types of cells: 1) cells with granules of about 350–550 mμ in diameter, 2) cells with small granules of 200–250 mμ in diameter and globules with a cristal-like arrangement containing cylinders with a diameter of about 960 Å and 3) cells containing small granules only. The AA. discuss the ultrastructural changes of the gland and the modifications of sexual secondary characters (S.S.C.) in animals given Cyproterone acetate (1/2 mg every three days). The animals have been treated for a period of time varying between 3 and 5 months, starting in October-November, when S.S.C. begin to develop again. At the end of the treatment the newths showed a loss of S.S.C., and the ultrastructure of the adenohypophysis resembled that of castrated animals, i.e.: great swelling of R.E.R. and partial degranulation of glycoprotein secreting cells which contain the 200 mμ granules and the globules. The S.E.R. showed also swelling and hyperactivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307431
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  • 6
    Electronic Resource
    Electronic Resource
    The rapid quantitative analysis of codeine phosphate drug substance by reversed-phase high-performance liquid chromatography (1985)
    Springer
    Chromatographia 20 (1985), S. 289-292 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Reversed-phase ; Codeine phosphate drug substance ; Opium alkaloids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A rapid procedure for the evaluation of codeine phosphate drug substance derived from poppy straw or opium concentrate is described. The gradient HPLC procedure employs a pH 2.5 phosphate buffer, methanol and acetonitrile mobile phase at 40°C and a μBondapak C-18 column. Eight opium alkaloids, including the four major alkaloids, are separated from each other. The paper describes details of the assay procedure and presents data documenting the linearity, specificity, precision, and sensitivity of the method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02310385
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  • 7
    Electronic Resource
    Electronic Resource
    The new generation of L. C. columns; A critical evaluation of their systems compatibility and performance (1985)
    Springer
    Chromatographia 20 (1985), S. 335-342 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; External variance determinations ; Column and system compatibility ; Microbore and fast LC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The demand for faster, more economic and convenient high-efficiency LC analyses has led to the development of microbore and fast columns. However, to utilize their benefits, the low column dispersions produced by these new technologies necessitate low external variances in the total system. This paper describes the determination of the external variance of microbore, fast and conventional LC systems by a column method. Compatibility with the relevant column technologies is evaluated by a method involving the comparison of the external and column dispersions. Direct comparison of chromatograms of an 8-component mixture, on a set of columns of various diameters and lengths, shows the relative speed, economy and sensitivity obtainable in practice. The problem of reduced sample loadability is considered and the practical solution of peak compression illustrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02269058
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  • 8
    Electronic Resource
    Electronic Resource
    Retention prediction system coupled with a multichannel ultraviolet detector for the identification of polycyclic aromatic hydrocarbons (1985)
    Springer
    Chromatographia 20 (1985), S. 351-356 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Retention prediction system ; UV multichannel detector ; Polycyclic aromatic hydrocarbons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The retention prediction system assisted by a micro-computer coupled with an UV multichannel detector has been investigated in the separation of polycyclic aromatic hydrocarbons. The method offered very precise identification of components in NBS-SRM-1647 sample without any standard reference substances. It has been proven that the system makes very precise, non-pollutive and convenient environmental analysis possible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02269061
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  • 9
    Electronic Resource
    Electronic Resource
    Fast determination of tetracycline antibiotics in different media by high-performance liquid chromatography (1985)
    Springer
    Chromatographia 20 (1985), S. 313-322 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Tetracyclines antibiotics ; Quantitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The use of high-performance liquid chromatography (HPLC) for the identification and determination of tetracycline antibiotics is reviewed. HPLC chromatograms provide fast identification by retention time, tR, and precise quantitation by measurement of peak height or peak area. For separation of tetracycline compounds, most HPLC methods use reversed-phase C18 or C8 columns and UV detection. The HPLC solvent system should have a pH of about 6 to prevent steric changes in the tetracycline molecule. For accurate quantitation it is necessary to avoid tailing and this is accomplished by adding a zwitter ion to the solvent system. Methanol and acetonitrile are frequently used as organic modifiers in these solvent systems. In a single analysis, HPLC methods can be used to separate as many as nine or ten commercially used tetracycline compounds and to determine four to five tetracyclines in commercial tetracycline preparations or in biological fluids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02310389
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  • 10
    Electronic Resource
    Electronic Resource
    Direct analysis of fenvalerate isomers by liquid chromatography. Application to formulation and residue analysis of fenvalerate (1985)
    Springer
    Chromatographia 20 (1985), S. 376-378 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Resolution of optical isomers ; Chiral column ; Pyrethroids ; Fenvalerate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The separation of the optical isomers of fenvalerate [cyano(3-phenoxyphenyl)methyl 2-(4-chlorophenyl)-3-methylbutyrate] has been carried out by high-performance liquid chromatography (HPLC) on a chiral column with (R)-N-3,5-dinitrobenzoyl-phenyl-glycine (DNBPG) covalently bonded on aminopropyl silica and eluted with mixtures of methanol, 2-propanol, and hexane. The system was applied to the analysis of Pydrin®, an emulsifiable concentrate formulation of fenvalerate, and to the residue analysis of fenvalerate in milk samples. For the analysis of Pydrin® the only requirement was the proper dilution of Pydrin® with hexane. For the analysis of residues in milk, fenvalerate was extracted with hexane after precipitating the milk proteins with acetonitrile and removing the precipitate by filtration; the hexane extract was concentrated to small volume and filtered before being analyzed by HPLC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02269066
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