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  • Triticum aestivum  (853)
  • Springer  (853)
  • Nature Publishing Group
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  • 1
    Electronic Resource
    Electronic Resource
    Transmission of somaclonal variation in wheat (1985)
    Springer
    Euphytica 34 (1985), S. 525-537 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; somaclonal variation ; chromosomal aberrations ; wheat breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Transmission of somaclonal variation was studied in callus derived (SC1) plants of wheat, Triticum aestivum L. Second (SC2), third (SC3) and fourth (SC4) generations were compared with those obtained from embryo culture (E1-E4). SC2 generation had significantly lower grain yield (−37.3%), reduced thousand kernel weight (−7.8%) and shorter height (−2.1%) than that of E2, and included 5.7% seed-sterile and 15.0% partially seed-sterile plants. SC3 generation showed reduced yield (−12.6%), lower thousand kernel weight (−4.9%) and shorter plant height (−1.9%) than E3 generation. SC4 progeny of a short height, partially sterile SC1 variant included aberrant plants: a haploid, few aneuploids, mixoploids, some showing multiploidy, spindle abnormalities, multivalents, bridges and fragments, and tillers having supernumerary spikes with branched rachis. Somaclonal variation resulted from genome instability in callus, and likely involved transposable elements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022950
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  • 2
    Electronic Resource
    Electronic Resource
    In vitro techniques for selecting wheat (Triticum aestivum L.) for Fusarium-resistance. II. Culture filtrate technique and inheritance of Fusarium-resistance in the somaclones (1996)
    Springer
    Euphytica 91 (1996), S. 341-349 
    Details
    ISSN: 1573-5060
    Keywords: Fusarium resistance ; Fusarium spp. ; wheat ; Triticum aestivum ; culture filtrate ; in vitro selection ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Calli of resistant, intermediary and susceptible wheat (Triticum aestivum L.) varieties were selected using culture filtrates of Fusarium graminearum and F. culmorum and the regenerants were evaluated for resistance up to R3. Czapek-Dox broth medium was inoculated with mycelia of Fusarium isolates and incubated for 2–6 weeks. Filtrates were added to MS callus growing medium, then 5 weeks-old calli were transferred onto this medium (MST) for 4–5 weeks. MST containing 30% filtrate was found to be suitable for selection. Resistant calli were transferred again to fresh MST for further two selection cycles. The surviving calli produced less fertile regenerated lines (R0) than the non-selected ones. Among 18 R1 lines tested for Fusarium-resistance in the seedling stage by artificial inoculation in the greenhouse, two (11.1%) were significantly more resistant, one (5.6%) was more susceptible than the original cultivar and the rest (83.3%) behaved similarly to the donor plants. Among unselected R3 lines of three varieties, practically the same number of resistant plants were found as among the related selected ones. When the R3 selfed generations obtained through double-layer and culture filtrate selection techniques were tested for Fusarium-resistance, 35.7% of the lines were found to be more resistant than the original cultivars, none was more susceptible and 64.3% had a reaction similar to that of the source materials. Thus, inheritance of the disease reaction was not stable in all cases. Success of in vitro selection for Fusarium-resistance depended also on the genotype, and toxin analysis showed that although being effective, the selective media contained deoxynivalenol only exceptionally. In selecting wheat for Fusarium-resistance in vitro, the culture filtrate technique proved better than the double-layer procedure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033096
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  • 3
    Electronic Resource
    Electronic Resource
    In vitro techniques for selecting wheat (Triticum aestivum L.) for Fusarium-resistance. I. Double-layer culture technique (1991)
    Springer
    Euphytica 57 (1991), S. 251-257 
    Details
    ISSN: 1573-5060
    Keywords: Fusarium resistance ; Fusarium spp. ; wheat ; Triticum aestivum ; double-layer technique ; in vitro selection ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Calluses of spring and winter wheats (Triticum aestivum L.) were selected for Fusarium resistance in vitro, using the double-layer culture technique. Potato-dextrose agar medium in vials was inoculated with mycelia of Fusarium graminearum and F. culmorum. After one week, fungal cells were killed by autoclaving and the agar medium containing the thermostable toxic metabolites was overlayered with MS callus-growing medium. Later, wheat calluses were placed on the upper medium for 4–5 weeks, and from the surviving calluses plants were regenerated. R2 seedling populations from self-fertilized R1 plants of 4 varieties were tested for Fusarium resistance by artificial infections in the greenhouse, and 3% of the regenerated R2 plants have been found to be more resistant than the original cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039671
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  • 4
    Electronic Resource
    Electronic Resource
    Searching for DNA introgressed from wheat and for wheat-like grain proteins in a rice x wheat hybridization derivative (1993)
    Springer
    Euphytica 72 (1993), S. 177-182 
    Details
    ISSN: 1573-5060
    Keywords: intergeneric introgression ; Oryza sativa, prolamins ; RFLP ; Triticum aestivum ; X Oryticum oryzoides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The DNA of a putative rice x wheat hybridization derivative (X Oryticum oryzoides) from China, the DNA of its parental rice cultivar and the DNA of a wheat line were digested with ten different restriction endonucleases, resolved by agarose electrophoresis, Southern blotted and hybridized using genomic wheat DNA as a probe. Phenol extracted, ethanol and cetyltrimethylammonium bromide precipitated DNA of the putative hybrid showed a restriction fragment length polymorphism (RFLP) different from that of the parental rice. When the DNA was further purified by Qiagen chromatography, the RFLP differences were not detected. Hence the apparent RFLP differences were probably due partial digestion of the less pure DNA preparations by the restriction endonucleases. No real introgressed fragments from wheat genome could be shown. The HpaII/MspI sites were more frequently digested with MspI than with HpaII in rice and hybridization derivative DNA, but the sites were evidently more frequently methylated in wheat DNA. Thus, in terms of methylation of the DNA, the hybridization derivative was much more like the rice parent than the wheat parent. The hybridization derivative showed a single endospermal protein (mass 19 kg mol-1) not detected in the parental rice cultivar. This minor protein was soluble in buffered 50% isopropanol and precipitable with methanol. The results indicate that there are no or only short introgressed sequences from wheat in the rice/wheat derivative, a result which might be considered in breeding efforts with the hybrid derivative.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034155
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and analysis of a cDNA clone encoding the small subunit of ADP-glucose pyrophosphorylase from wheat (1993)
    Springer
    Plant molecular biology 23 (1993), S. 23-33 
    Details
    ISSN: 1573-5028
    Keywords: wheat ; Triticum aestivum ; cDNA clone ; ADP-glucose pyrophosphorylase ; small-subunit ; starch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A full-length cDNA clone from hexaploid bread wheat, encoding the small subunit of ADP-glucose pyrophosphorylase, has been isolated from an endosperm cDNA library. The cDNA insert has an open reading frame which encodes a protein of 473 amino acids (52.1 kDa). The presence of a chloroplast/amyloplast transit peptide of 22 amino acids is proposed. The deduced amino acid sequence exhibits a high degree of homology with the small subunit ADP-glucose pyrophosphorylase proteins from rice (with 90% of identical amino acids) and potato (with 86% of identical amino acids) and contains conserved sequence elements which are thought to represent the substrate binding and allosteric activator sites. The genes are organised as single-copy loci on chromosomes 7A, 7B and 7D in the wheat genome and are highly expressed during grain development. Homologous transcripts are expressed in leaves and roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021416
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  • 6
    Electronic Resource
    Electronic Resource
    Path coefficient analysis of the effects of stripe rust and cultivar mixtures on yield and yield components of winter wheat (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 666-672 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Puccinia striiformis ; Diversity ; Competition ; Path analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four club wheat cultivars and three two-component cultivar mixtures, planted at five frequencies, were grown in three environments in both the presence and absence of stripe rust. The effect of stripe rust on wheat yield was through the yield components, with weight of individual seed being the component most affected by rust. In some cases, yield component compensation was indicated by the presence of negative correlations among the yield components. Path analysis of the yield components revealed that components with the highest correlations to yield also had the largest direct effects on yield. Of the yield components, number of heads per unit area exerted the largest direct influence on yield. The direct effects of number of seeds per head and weight of individual seed were similar, although number of seeds per head was more important in the absence of rust than in its presence. The pure stands and mixtures differed considerably with respect to correlation coefficients, but were very similar for direct effects of yield components on yield. Most of these discrepancies were due to opposing indirect effects, which were not evident from correlation coefficients alone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226087
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  • 7
    Electronic Resource
    Electronic Resource
    The bread wheat races of bunt represented in the Australian bunt collection (1987)
    Springer
    Euphytica 36 (1987), S. 577-580 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Tilletia laevis ; Tilletia tritici ; common bunt ; physiologic races ; resistance ; Bt genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Australian Bunt Collection, obtained from infected crops between 1962–1977, has been classified into races, based on the differential reactions to ten known bunt resistant genes. Eight physiologic races of Tilletia laevis and three of T. tritici were identified. No race had virulence against the genes Bt3, Bt5, Bt8, or Bt10, of wheat. Consequently four major genes are available for breeding Australian wheat cultivars with resistance to common bunt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041505
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  • 8
    Electronic Resource
    Electronic Resource
    Fusarium globosum from subtropical Japan and the effect of different light conditions on its conidiogenesis (1999)
    Springer
    Mycoscience 40 (1999), S. 1-9 
    Details
    ISSN: 1618-2545
    Keywords: BLB light ; conidiogenesis ; Fusarium globosum ; subtropical Japan ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The second report ofFusarium globosum is based on strains isolated from wheat in Ishigaki Island, Okinawa, in subtropical Japan. Morphological features of the Japanese isolates are described. These strains show different reactions in conidiogenesis to light conditions. Under continuous BLB light, falcate sporodochial conidia are typically induced, but production of aerial globose conidia is suppressed. In two of the strains, clavate conidia became longer under BLB light. Application of both, complete darkness and continuous BLB light, is recommended as standard light conditions to cultureFusarium isolates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02465667
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  • 9
    Electronic Resource
    Electronic Resource
    The 5S DNA units of bread wheat (Triticum aestivum) (1992)
    Springer
    Plant systematics and evolution 183 (1992), S. 183-194 
    Details
    ISSN: 1615-6110
    Keywords: Poaceae ; Triticum aestivum ; 5S DNA sequences ; chromosomal5S Dna loci ; wheat evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A collection of 44 cloned 5S DNA units fromTriticum aestivum cv. ‘Chinese Spring’ were grouped into 12 sequence-types based on sequence similarity and the respective consensus sequences were then produced. The relationship between these 12 consensus sequences (T. aestivum S 1-S 8 andT. aestivum L 1-L 4), together with two clones sequenced byGerlach andDyer, and the 5S DNA consensus sequences from diploidTriticum spp. were then determined by numerical methods. Both phenetic and cladistic analyses were carried out. The following wheat 5S DNA sequences were found to group with respective sequences from diploidTriticum spp.:T. aestivum S 4, S 6 withT. tauschii S;T. aestivum S 3 withT. monococcum S andT. monococcum S-Rus 7;T. aestivum L 1 andT. aestivum L-G&D withT. speltoides L;T. aestivum L 2, L 3 withT. tauschii L;T. aestivum L 4 withT. monococcum L andT. monococcum L-Rus 12. The analyses suggested that 5 out of the 65S Dna loci present in wheat were identified at the sequence level. The locus that could not be identified in this analysis was the5S Dna-B 1 locus. A group ofT. aestivum sequences (T. aestivum S 1, S 7, S 8, S-G&D) were found to be distinct from the other 5S DNA sequences in the data base. The existence of the distinct group of 5S DNA sequences suggests that there is a gap in our current understanding of wheat evolution with respect to the5S Dna loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00940802
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  • 10
    Electronic Resource
    Electronic Resource
    Heading date and resistance to septoria tritici blotch in wheat not genetically associated (1999)
    Springer
    Euphytica 106 (1999), S. 63-68 
    Details
    ISSN: 1573-5060
    Keywords: disease assessment ; maturity ; resistance ; Septoria tritici ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nineteen cultivars, with large differences in heading date, were evaluated for their response to septoria tritici blotch in two experimental setups in Njoro, Kenya. Due to the more or less constant temperatures during the growing season and the overhead irrigation applied the epidemic conditions were similar over the whole observation period for the early and late cultivars. In experiment 1 the cultivars were assessed for disease severity at the same moment irrespective of the developmental stage, while in experiment 2 the cultivars were assessed at the same developmental stage. Measured at the same time, the disease severity was highest in the early maturing cultivars and lowest in the late maturing cultivars (r = –0.78). When assessed at the same development stage the disease build up was independent of heading date (r = –0.10) but strongly dependent on resistance level. There were no indications that early heading cultivars were more susceptible than late heading cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003593218761
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