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  • Articles  (826)
  • Rat  (826)
  • Springer  (826)
  • American Chemical Society (ACS)
  • 101
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    Cell & tissue research 296 (1999), S. 235-246 
    ISSN: 1432-0878
    Keywords: Key words Transplantation ; Parkinson’s disease ; CNS fetal development ; CNS differentiation ; Neurotrophic factors ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have previously shown that a combination of the cytokines interleukin (IL)-1, IL-11, leukemia inhibitory factor (LIF), and glial cell line-derived neurotrophic factor (GDNF) can convert rat fetal (E14.5) mesencephalic progenitor cells into tyrosine hydroxylase (TH)-immunoreactive (ir) neurons in vitro. The experiments described here characterize the mesencephalic progenitor cells and their cytokine-induced conversion into dopamine (DA) neurons. For all experiments, we used bromodeoxyuridine (BrdU)-ir cultures of (E14.5) mesencephalic progenitor cells that had been expanded at least 21 days. We first demonstrated that IL-1 induced DA neuron conversion in mesencephalic progenitors, but not in striatal progenitors (P〈0.001). Thus, these cells should be classified as lineage-restricted progenitors, and not omnipotent stem cells. To further characterize cell populations in these cultures, we used monoclonal antibodies against Hu (an early marker for neurons), growth-associated protein (GAP)-43 (a marker for neuronal process extension), TH (a marker for DA neurons), and glial fibrillary acidic protein (GFAP, a marker for astrocytes). We assessed (E14.5) mesencephalic progenitor cell cultures (plated at 125,000 cells/cm2) incubated in the cytokine mixture (described above) or in complete media (CM, negative control). Following 7 days incubation, GFAP-positive cells formed a nearly confluent carpet in both types of cultures. However, numbers of Hu-ir and GAP-43-ir cells in the cytokine-incubated cultures far exceeded those in CM-incubated controls (P=0.0003, P=0.0001, respectively), while numbers of TH-ir cells were 58-fold greater in the cytokine-incubated cultures versus CM-incubated controls. The TH phenotype persisted for 7 days following withdrawal of the differentiation media. Numerous double-labeled cells that were BrdU-ir and also TH-ir, or Hu-ir and also TH-ir, were observed in the cytokine-incubated cultures. These data suggest that cytokines ”drive” the conversion of progenitor cells into DA neurons.
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  • 102
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    Cell & tissue research 143 (1973), S. 107-116 
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Rat ; Pituicytes ; Organotypic culture ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neural lobes of adult rats have been explanted and organ-cultured for 5–10 days. While perivascular cells and neurosecretory fibers undergo a progressive degeneration, scattered pituicyte-like cells are observed mostly associated with each other in number of two or three cells for group. Cultured pituicytes are quite similar to in vivo pituicytes except for some particular features as the shape of the nucleus and the number of dense bodies. Furthermore they share with the in vivo pituicytes the phagocytic capacity which appears even increased as far as neurosecretory fibers are concerned. Finally, degenerating pituicytes and free lipid droplets are observed in the intercellular spaces: the significance of these facts is discussed and tentatively interpreted. The organotypic culture of the neural lobe seems to represent an experimental model useful to obtain a relatively pure population of pituicytes.
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  • 103
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    Cell & tissue research 143 (1973), S. 505-515 
    ISSN: 1432-0878
    Keywords: Endoplasmic reticulum ; Paracervical ganglion (Rat) ; Neurosecretion ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two new types of structure of the cytoplasm of an autonomic neuron were described: (1) Inclusions of the rough ergastoplasmic reticulum, which were named “ergastosomes”, contain finely granular material with moderately electron opaque staining with both glutaraldehyde-OsO4 and KMnO4 fixations. A short comment was made on the nature of the contents of the ergastosomes. (2) Tubular formations of endoplasmic reticulum were found in the axons and/or dendrites of the paracervical ganglion.
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  • 104
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    Cell & tissue research 146 (1973), S. 453-461 
    ISSN: 1432-0878
    Keywords: Hypophysis ; Primordium ; Rat ; Organ culture ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To see whether adenohypophysial tissue has the capacity of self-differentiation, Rathke's pouch together with part of the neurohypophysial primordium was isolated from 12-(Group A), 14- (Group B) and 15-day-old (Group C) rat fetuses and cultivated for 9, 6 and 5 days, respectively. Electron microscopic examination of these explants showed many adenohypophysial cells containing electron dense granules, indicative of cytodifferentiation in all groups. Three cell types were distinguishable on the basis of the size of their granules. Variable amounts of neurohypophysial tissue were seen in some explants of Groups B and C, but not A. This “pars nervosa” contained pituicytes with lipid-like inclusions in their cytoplasm but no neurosecretory axons. The adenohypophysial cells abutting on the pars nervosa were arranged rather regularly like cells of the pars intermedia which contained a few granules of about 200 mμ diameter. From these data it is concluded that in rats granule formation in the adenohypophysial primordial cells starts in the absence of hypothalamic neurosecretory substances. It is uncertain from this study whether these cells actually contain hypophysial hormones.
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  • 105
    ISSN: 1432-0878
    Keywords: LRF ; Hypothalamic localization ; Rat ; Ependyma ; Glia ; Light and electron microscopic Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Light and electron microscopic autoradiographic analysis of the medial basal hypothalamus of rat brains incubated for 30 and 60 minutes in tritiated luteinizing releasing factor (3H-LRF) revealed selective uptake in the form of silver grains over specialized ependyma (tanycytes) and perivascular glial cells in the palisade-contact zone of the median eminence. The functional capacity of tanycytes and glia are discussed in light of these data.
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  • 106
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    Cell & tissue research 144 (1973), S. 453-466 
    ISSN: 1432-0878
    Keywords: Lacrimal gland ; Rat ; Duct system ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The duct system of the rat exorbital lacrimal gland consists of intercalated ducts, interlobular ducts and excretory ducts. The morphological changes from one type of duct to the next are gradual. At the light microscopical level this consists of a change from a bilaminar epithelium in the intercalated ducts to an epithelium, consisting of approximately three layers — which may be pseudostratified — in the excretory ducts. The basal layer of the intercalated ducts consists of myoepithelial cells, whereas the inner epithelial cells may have both a secretory and an electrolyte transporting function. The interlobular duct epithelium contains many cells with deep infoldings of the basolateral plasma membranes and associated mitochondria, suggesting a similar function to the striated duct epithelium in salivary glands. Numerous basal cells in this epithelium have tentatively been interpreted as unusual myoepithelial cells. Nerve terminals have been observed in the ductal epithelium.
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  • 107
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    Cell & tissue research 150 (1974), S. 493-496 
    ISSN: 1432-0878
    Keywords: Serotonin ; Neurotransmitter ; Myenteric plexus ; Rat ; Histofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several recent studies suggested that serotonergic neuron-like elements are present in the guinea pig ileum. The present paper reports an extensive study of the digestive tract of the rat with the use of a histofluorescence technique. Administration of the serotonin precursor, tryptophan, associated with a monoamine oxidase inhibitor, did not allow histochemical demonstration of rapidly fading, yellow fluorescent, 6-hydroxydopamine-resistant neurons; conversely such neurons were readily detected in the brain. It is concluded that serotonergic neuron-like elements cannot be detected histochemically in the rat myenteric plexus area after chemical sympathectomy.
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  • 108
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    Cell & tissue research 151 (1974), S. 457-469 
    ISSN: 1432-0878
    Keywords: Juxtaglomerular apparatus ; Rat ; Adrenalectomy ; Renin and secretory granules ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Reaktion des juxtaglomerulären Apparates auf Adrenalektomie wurde untersucht. Im Stadium der Nebenniereninsuffizienz, sechs Tage nach der Operation, wurde quantitativ morphometrisch eine Abnahme der Volumendichte der Sekretgranula (von 32% auf 16%) festgestellt. Golgi-Apparat und Ergastoplasma proliferieren stark, wobei insbesondere die erweiterten Cisternen des Ergastoplasmas auffallen.
    Notes: Summary The response of the Juxtaglomerular apparatus to bilateral adrenalectomy was studied in rats. After the sixth postoperative day, symptoms of severe adrenal insufficiency had developed. In the Juxtaglomerular cells, the volume density of the secretory granules decreased from 32% to 16%. The Golgi apparatus and rough endoplasmic reticulum underwent considerable proliferation, the latter showing strikingly enlarged cisternae.
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  • 109
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    Cell & tissue research 151 (1974), S. 525-536 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex differences ; Gonadal hormones ; Karyometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adrenal glands of adult female rats are heavier than the glands of corresponding male rats. Postpubertal orchiectomy increases the adrenal weight, an effect restored by testosterone replacement. Under the same conditions ovariectomy of 8 weeks duration does not change the adrenal weight while estradiol replacement enhances the relative adrenal weight. Karyometric studies have shown that nuclei in the female zona fasciculata cells are larger (app. 18%) than those of the male. Similar but only slight differences (2%) were observed in the zona reticularis. Orchiectomy results in enlargement of cell nuclei within all zones of the adrenal cortex; testosterone replacement has the opposite effect. Ovariectomy of 8 weeks duration slightly enhances the volume of nuclei of the zona glomerulosa cells, has no effect on the nuclei of the zona fasciculata and reduces the volume of nuclei in the zona reticularis. Estradiol replacement reduces the volume of nuclei of the zona glomerulosa cells but increases nuclear volume in the zona fasciculata and in the zona reticularis. Thus testosterone has an inhibitory effect on the adrenal cortex of the rat while the physiologic effect of estradiol on the morphology of this gland, particularly on the zona fasciculata cells is rather dubious. The author wishes to thank Mrs. B. Westerska and Miss K. Siejak for excellent technical assistance.
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  • 110
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    Cell & tissue research 151 (1974), S. 537-547 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex differences ; Gonadal hormone ; Stereologic studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adult female rats have heavier adrenals than males. Orchiectomy increases the adrenal weight, an effect reversed by testosterone. Neither ovariectomy nor estradiol replacement has an effect on absolute adrenal weight. The stereologic analysis of the volume fractions of particular adrenocortical zones have shown that the volume fraction of the zona glomerulosa in females is smaller as compared with males. On the contrary, the volume fractions of the fasciculata and reticularis zones are higher in female than in male rats. In both sexes gonadectomy and gonadal hormone replacement has an effect on the volume fractions of particular adrenocortical zones, these changes being more pronounced due to orchiectomy or testosterone replacement. The adrenal cortex of all experimental groups contains a similar quantity of parenchymal cells. The cytoplasm volume of a single adrenocortical cell is higher in all zones of the female adrenals as compared with the male. Orchiectomy increases the volume of the cytoplasm in fasciculata and reticularis cells. This effect was reversed by testosterone. Neither ovariectomy nor estradiol replacement has an effect on the volume of the cytoplasm in the fasciculata and reticularis cells. These studies have shown that sex differences with regards to rat adrenal size and weight depend upon the “physiologic atrophy” of the male adrenal cortex, while neither ovariectomy (8 weeks after operation) nor estradiol replacement has a conspicuous effect on the morphologic changes of this gland.
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  • 111
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    Cell & tissue research 182 (1977), S. 505-511 
    ISSN: 1432-0878
    Keywords: Endothelium ; Cerebral artery ; Filaments ; Rod-shaped inclusion ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Endothelia of the anterior cerebral arteries in rats aged 1 to 3 days were studied. Thin (about 50–90 Å) and thick (about 100–110 Å) filaments are present in the endothelia. Numerous spherical- or rod-shaped bodies, measuring approximately 0.07 to 0.3 μm in diameter and up to 0.6 μm in length occur in the endothelial cells. These bodies contain a tubular structure. The diameter of the individual tubules is about 200 Å. The present observations suggest that spherical- or rod-shaped inclusions are first synthesized in the rough endoplasmic reticulum and thereafter these materials are transported into the Golgi complex for maturation. A small number of the inclusions, however, may originate directly from the rough endoplasmic reticulum and not pass through the Golgi apparatus.
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  • 112
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    Cell & tissue research 239 (1985), S. 651-655 
    ISSN: 1432-0878
    Keywords: Gap junctions ; cAMP ; Liver ; Freeze-fracturing ; Morphometry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Since cAMP has recently been reported to be a possible physiological modulator of cell-to-cell communication, we performed a quantitative freeze-fracture investigation on the hepatocyte gap junctions after administration of a membrane-permeant derivative of this cyclic nucleotide. For this purpose, male rats received two intraperitoneal injections of 100 mg dibutyryl cAMP/kg body weight with a time interval of 2.5 h. Litter mates were injected with saline only. Five hours after the start of the treatment, tissue blocks of the left lateral liver lobe were fixed by immersion and processed for freeze-fracture. By point counting on negatives projected on a square double-lattice test system the relative gap junctional area on contiguous hepatocyte membranes was determined. As compared to control animals, the proportion of the membrane area occupied by gap junctions in dibutyryl cAMP-treated liver parenchyma significantly increased from 4.9% to 6.1%. Within the gap junctions no changes in shape, particle density or packing pattern were observed. Possibly, the enlarged gap junctional area provides structural pathways for the integration of the response of hepatocytes to messages mediated by cAMP.
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  • 113
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    Cell & tissue research 171 (1976), S. 285-296 
    ISSN: 1432-0878
    Keywords: Prostate ; Rat ; Castration ; Nuclear alterations ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the nuclei of epithelial cells of the dorsal lobe of the rat prostate were studied 2, 3, 5, 7 and 21 days after castration. The nucleolus appears to undergo a progressive disorganisation with partial fragmentation and dispersion of its normal components. Changes in the nucleoplasm were primarily reflected by a condensation of chromatin, particularly along the nuclear membrane and adjacent to the nucleolus. Later, different types of intranuclear inclusions were observed. After 21 days, the nuclei were characterized by an irregular outline with large indentation. Within the nucleoplasm aggregates of coarse granular chromatin were found. No cell necrosis was observed, indicating that androgen deprivation results in a remodeling of the cell to a less active state with marked cellular alterations and cessation of secretion, but apparently with some of their basic functions still intact. Injections of testosterone completely reverse the castrated-induced alterations. The changes observed are assumed to be due to the withdrawal of the androgenic stimulus, with a direct influence on the secretory function of the cell. The findings support the view that the stimulating secretory effect of androgen is mediated via an intranuclear androgen receptor, probably located in the nucleolus-associated-chromatin. It is also proposed that the secretory function of the epithelial cells of the prostatic complex, initiated by androgens, may be regulated by an intranuclear secretory center.
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  • 114
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Populations of axonal granules ; Electron microscopy, morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In Fortsetzung einer früheren Arbeit wurde die Eminentia mediana von männlichen und weiblichen Ratten, sowie die trächtiger und durstender Tiere auf die Fragestellung hin untersucht, ob 1. die Eminentia mediana der erste Ort ist, wo die beiden Neurohormone Oxytocin und Vasopressin freigesetzt werden, und 2. ob sich die Axone in der externen Schicht der Eminentia mediana aufgrund der mittleren Durchmesser ihrer Granula in verschiedene Populationen differenzieren lassen. Bei männlichen und weiblichen Kontrolltieren haben die Oxytocin-bzw. Vasopressin-granula in der Eminentia mediana bereits ihre endgültige Größe erreicht; ihr mittlerer Durchmesser ändert sich nicht mehr wesentlich bis zum Hypophysenhinterlappen. Allerdings gibt der unterschiedliche prozentuale Anteil beider Populationen in der Eminentia mediana, in Zusammenhang mit den früher bestimmten Werten für die jeweiligen Kerne und die Neurohypophyse, Hinweise auf gewisse Unterschiede im turnover beider Hormone bei männlichen und weiblichen Tieren. Bei graviden und durstenden Ratten findet sich eine scheinbare Abnahme der Granula Durchmesser in den oxytocin-bzw. vasopressinführenden Axonen. In Verbindung mit sehr charakteristischen morphologischen Befunden, besonders bei langdurstenden Tieren, sprechen die morphometrischen Ergebnisse dafür, daß in der Eminentia mediana bereits Neurohormon freigesetzt wird, wenn eine schnelle Deckung des peripheren Bedarfes notwendig ist. Die Axone mit Granula, die kleiner sind als 115 nm, lassen sich in der internen Schicht der Eminentia mediana aufgrund der mittleren Durchmesser ihrer Granula in vier Populationen aufteilen. Ihre mittleren Granula-Durchmesser liegen bei etwa 84 nm, 96 nm, 103 nm und 110 nm. Dieselben vier Populationen finden sich sowohl in der Zona palisadica als auch in der perikapillären Schicht der Eminentia mediana. Im Unterschied zur Zona interna findet sich aber in diesen beiden Schichten noch eine fünfte Population von Axonen mit einem mittleren Granula-Durchmesser von etwa 70–75 nm. Der mengenmäßige Anteil dieser fünf Populationen aber ist unterschiedlich in der Zona palisadica und in der perikapillären Schicht einerseits und in den verschiedenen Tiergruppen andererseits. Einige morphologische Befunde an den Tanycyten werden im Zusammenhang mit der neueren Literatur diskutiert.
    Notes: Summary In connection with previous studies (Krisch, 1974), a morphologic analysis of the median eminence of male, female, pregnant, and thirsting rats has been made, in order to evaluate: 1. whether the median eminence is an additional area where oxytocin and vasopressin may be released from neurosecretory axons in the zona interna; 2. whether it is possible to distinguish different populations of axons according to the mean diameters of their granules in the external zone of the median eminence. In the zona interna of the male and the female control animals, the oxytocin-and vasopressin-containing granules have already reached their final diameters, i.e. their size remains constant until they reach and are stored in the neurohypophysis. However, the relative proportions of these populations in the fibre layer of the median eminence indicate certain differences between male and female animals, concerning the turnover of oxytocin and vasopressin. In pregnant, as well as in thirsting animals, there is an apparent decrease of the mean granule diameters in oxytocin and vasopressin-containing axons. In particular in the latter group, ballooned axons in the fibre layer of the median eminence, containing disintegrating granules, tubular structures filled with electron dense material, and fine-flocculent material in the axoplasm, together with the morphometric results, support our concept that in case of highly increased peripheral demand the contents of the mature, large granules are released into the axoplasm of the fibre layer in the median eminence. In addition, axons containing granules smaller than 115 nm may be classified into four populations with mean diameters of 84 nm, 96 nm, 103 nm, and 110 nm. The same populations are found in the palisade zone of the median eminence as well as in the pericapillary zone, but in contrast to the zona interna there is a fifth population of axons containing very small granules with a mean diameter of about 70–75 nm. The relative proportions of these five populations differ between the zona palisadica and the pericapillary zone on the one hand and between the experimental groups on the other hand. Some morphological findings concerning the tanycytes are discussed with respect to previously published data.
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  • 115
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    Cell & tissue research 164 (1975), S. 447-456 
    ISSN: 1432-0878
    Keywords: Protein secretion ; Protein synthesis ; Salivary glands ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of pilocarpine and food uptake on the rate of incorporation of [3H]-leucine in vivo was measured by means of quantitative radioautography in three exocrine cells of the rat: the acinar and the granular duct cells of the submandibular and the acinar cells of the parotid gland. The three cell types react differently. The submandibular acinar cells showed a decrease in incorporation rate after pilocarpine administration but not after feeding. The incorporation rate of the granular duct cells of the submandibular gland remains constant after both stimulations. The acinar cells of the parotid gland show an increase in incorporation rate of [3H]-leucine in response to both. The contrast between the submandibular and the parotid gland could also be demonstrated radiobiochemically, the results reflecting the incorporation rates of the acinar cells of both glands, giving no information on the contribution of other cell types. The decrease in incorporation rate of the submandibular gland acinar cells is accompanied by a shift of polyribosomes towards monomers.
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  • 116
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    Cell & tissue research 164 (1975), S. 457-466 
    ISSN: 1432-0878
    Keywords: Protein secretion ; Protein synthesis ; Salivary glands ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary After stimulation of the protein secretion by pilocarpine or feeding the rate of incorporation of [3H]-leucine increases in the acinar cells of the parotid gland of the rat while the secretory cells of the submandibular gland show a moderate decrease (Kuijper et al., 1975b). Since the rate of labelled amino acid incorporation depends on the specific radioactivity of the amino acid used, which is not easy to determine in vivo, experiments in vitro were performed to get an idea of the influence of this factor on the measured changes in [3H]-leucine incorporation. In vitro both cell types showed a more pronounced but essentially identical reaction as in vivo. Since in these experiments the specific radioactivity of the extracellular leucine is the same whether fragments of stimulated or unstimulated glands incorporate the radioactive amino acid, the increase of incorporation in the parotid and the decrease in the submandibular cells cannot be ascribed to differences in specific radioactivity of leucine, unless the intracellular leucine pool should show great differences between secreting and non-secreting cells. However, in vitro the submandibular gland cells under both conditions appear to use the extracellular leucine for their protein synthesis (or a small compartmentalized pool in rapid exchange with the extracellular pool). In the parotid cells the whole intracellular pool showed such a rapid exchange with the extracellular one that for practical reasons one may say that these cells, too, rely on the extracellular specific radioactivity of leucine in their protein synthesis. We conclude that the rat parotid gland cells show a rapid and substantial increase of protein synthesis after stimulation of their enzyme secretion, while the submandibular gland cells do not.
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    Cell & tissue research 164 (1975), S. 435-446 
    ISSN: 1432-0878
    Keywords: Protein secretion ; Protein synthesis ; Salivary glands ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In untreated, fasting animals the cells of the serous demilunes of the sublingual gland incorporate [3H]-leucine at a higher rate than any other of the 5 main cell types of the 3 major salivary glands. The acinar cells of the submandibular and the mucous cells of the sublingual gland show intermediate values, while the cells of the granular ducts of the submandibular and the acini of the parotid gland have a low rate of incorporation. In fasting animals extrusion of newly synthesized protein starts early in the cells of the serous demilunes. It starts between 4 and 7 hrs after [3H]-leucine injection in the acinar cells of the submandibular gland, while the other cell types did not lose substantial amounts of labelled (glyco)protein within 7 hrs. The secretion of protein is stimulated by the cholinergic drug pilocarpine in all but one of the 5 types of salivary gland cells studied. The acinar cells of the submandibular gland react strongly, the granular duct cells less strongly. Still less are the reactions of the acinar cells of the parotid and of the mucous cells of the sublingual gland. The cells of the serous demilunes of the latter appear to be insensible to pilocarpine. The effect of food uptake on secretion does not differ from pilocarpine stimulation, with one exception: the acinar cells of the parotid gland react more strongly on food uptake than on cholinergic stimulation.
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  • 118
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    Cell & tissue research 173 (1976), S. 529-542 
    ISSN: 1432-0878
    Keywords: Capillaries ; Cerebral cortex ; Endothelial cells ; Intraendothelial lumen ; Interendothelial lumen ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Developing blood vessels in rat cerebral cortex were studied at a number of stages between 3 and 28 days postnatal, in an attempt to obtain data on the mechanisms by which the lumen is established within cords of mesodermal cells. A combination of techniques was utilized in an attempt to elucidate these mechanisms. These were: (a) aldehyde fixation and block staining with phosphotungstic acid; (b) aldehyde perfusion followed by perfusion of a lead solution and post-fixation in osmium tetroxide; (c) conventional preparation of tissue with aldehyde and osmium fixation. Support for interendothelial lumen formation was readily forthcoming, including vessels with junctions between two or more endothelial cells cut transversely. There was some support for intraendothelial lumen formation, in the form of “seamless” endothelial cells. Other features noted included the presence of free ribosomes and vacuoles in the endothelial cells, endothelial flaps, sprouts and tendrils, intraluminal debris, endothelial degeneration and a junction with a nonendothelial cell. Large numbers of endothelial vacuoles were noted, many of them occurring at the abluminal edge of the cells. These vacuoles may be involved in the formation of intraendothelial lumina and also in the enlargement of both types of lumina. This study provides evidence that besides the well-established inter-endothelial lumen formation, intraendothelial mechanisms may also be operative in rat cerebral cortex. The techniques employed in this study offer the potential for clarifying these and related issues.
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  • 119
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    Cell & tissue research 168 (1976), S. 261-270 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Exercise ; Rat ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Four groups of male rats were exercised for periods of 2, 4, 6, and 8 weeks with controls in each group. As a result of chronic exercise there was an increase in the width of the zona fasciculata of the adrenal cortex. Also, there was an increase in the number and size of the mitochondria, and an increase in the quantity of smooth endoplasmic reticulum, and during the first 4 weeks of exercise an increase in the number of lipid droplets in the zona fasciculata. The close relationship between the smooth endoplasmic reticulum and the mitochondria, and the relationship between the smooth endoplasmic reticulum and the lipid droplets suggests a possible means for a transport mechanism for movement of precursors between these organelles.
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    Cell & tissue research 168 (1976), S. 303-314 
    ISSN: 1432-0878
    Keywords: Supraependymal cells ; Third ventricle ; Hypothalamus ; Hypophyseotropic area ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The supraependymal cells (SEC) are a normal component of the wall of the cerebral ventricles. In the hypothalamic area of the third ventricle they are restricted, in healthy animals, to the ependymal projection of the hypophyseotropic area. Here the SEC show great polymorphism. In addition to bipolar, multipolar and stellate or spider-like cells, transitional forms between these types can be seen. Their perikarya and processes can either remain at some distance from the ependyma or be in close contact with it. The processes may protrude between the ependymal cells or show surface differentiations that resemble the thin cytoplasmic folds of the mesenchymal wandering cells. Considering this and the variations in the number of cells, for example during the ovarian cycle, the SEC can be interpreted as mesenchymal cells, probably related to microglial cells of the subependymal layer. It is suggested that the SEC have a phagocytotic function and may be involved in the normal renewal of the ependyma. A definitive explanation for the restriction of the SEC to the hypophyseotropic area as well as the elucidation of their function remain to be found.
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  • 121
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    Cell & tissue research 176 (1977), S. 431-443 
    ISSN: 1432-0878
    Keywords: Cornea ; Rat ; NaK-ATPase ; K-NPPase ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The sodium-potassium activated adenosine triphosphatase (NaKATPase) activity of the rat cornea was investigated histochemically using a Pb2+-precipitation technique in which adenosine triphosphate (ATP) is used as substrate and two methods for potassium-dependent para-nitrophenyl-phosphatase (K-NPPase) activity. With all the three techniques used it was demonstrated that the sodium-potassium-activated adenosine triphosphatase (NaK-ATPase) activity is localized in the cell membranes of the endothelium whereas a much weaker activity was observed in the epithelium. When the Pb2+-technique was used, the epithelial cell membranes showed a weaker reaction in the presence of ouabain. This activity was only Mg2+-dependent and was presumably due to an Mg2+-dependent ATPase. The validity of the histochemical techniques for NaK-ATPase activity is discussed. The results emphasize the importance of the endothelium as the main site of Na+ transport in the cornea. Small amounts of the enzyme are also present in the epithelium, which seems to be rich in Mg2+-ATPase. Provided that careful controls are performed, all the methods give consistent results in the cornea.
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  • 122
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    Cell & tissue research 176 (1977), S. 131-142 
    ISSN: 1432-0878
    Keywords: Pineal gland ; Graft ; Ultrastructure ; Innervation ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pineal glands were grafted under the kidney capsule of mature male rats for periods of 20, 40, 60 and 100 days. Each grafted gland was then excised and divided into two halves. One half was processed for conventional electron microscopy and the other was fixed in aldehydes and then incubated in a zinc iodide-osmium tetroxide mixture at pH 4.4 (A-ZIO-4.4). During the forty days following the operation pinealocytes showed the typical ultrastructural features associated with cells with a high protein and/or peptide secretory activity. On the other hand, during this period, the number of granular vesicles decreased progressively. From day 40 on, the grafted pinealocytes lacked granular vesicles. During the second half of the experimental period the ultrastructure of the pinealocytes indicated that their secretory activity was considerably decreased. During the acute phase of the experimental period numerous structures regarded as the tip of growing axons as well as typical nerve fibres appeared around blood vessels and within the parenchyma of the grafted gland. In the transplanted tissue obtained 60 and 100 days after the operation the growth cones were scarce, whereas typical nerve endings became numerous. These endings contained small clear vesicles which reacted positively when the tissue was treated with A-ZIO-4.4. The secretory activity of the grafted pineal gland and the nature of the nerve fibres which innervate the graft are discussed. The authors wish to thank Mrs. E.M. Rodríguez de Calderón for her valuable help
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  • 123
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    Cell & tissue research 168 (1976), S. 117-131 
    ISSN: 1432-0878
    Keywords: Retino-hypothalamic projection ; Suprachiasmatic nucleus ; Rat ; Axonal iontophoresis/cobalt precipitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The technique of cobalt sulfide precipitation combined with Timm's sulfide-silver method for intensification of heavy metals was used to delineate the retino-hypothalamic projection of the rat. Freshly isolated rat brains were dissected and a solution of cobaltous chloride was applied to one of the cut optic nerves. Sixteen hours later, after cobalt ions had passed into the brain along the entire length of the optic fibers, the preparation was treated with ammonium sulfide to precipitate the cobalt as cobalt sulfide. In thick light microscopic sections, cobalt-filled axons were visualized as black fibers against a light gold background. Such fibers were observed to leave the posterior medial portion of the optic chiasm and, after arching dorsally, to project into the posterior fifth of the suprachiasmatic nucleus (SCN), as well as into the rostral part of the arcuate nucleus. Neither bifurcation of these axons nor looping of the axons back to the chiasm was seen. Most fibers projected to the SCN contralateral to the filled nerve, but the projection represented less than 0.1 % of the total number of fibers in one optic nerve. These observations are considered to be graphic evidence of a retino-hypothalamic projection. The interpretation of the cobalt method is discussed, as are the functions of the connections that have been observed.
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  • 124
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    Cell & tissue research 177 (1977), S. 287-290 
    ISSN: 1432-0878
    Keywords: Liver ; Intralobular nerves ; Rat ; Cobalt and Procion Yellow staining
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In a recent study (Skaaring and Bierring, 1976) we found cholinesterase positive nerve-like structures in the lobules of rat liver, and scanning electron microscopy revealed cords having a distribution pattern similar to that of the cholinesterase-positive structures. To obtain further evidence for an intralobular nerve supply the methods of cobalt and Procion Yellow nerve staining (Stretton and Kravitz, 1968; Iles and Mulloney, 1971; Pitman, Tweedle and Cohen, 1972) were adapted, iontophoretic introduction of the dyes being attempted through cut axonal ends in the surface of small excised blocks of rat liver.
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  • 125
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    Cell & tissue research 136 (1973), S. 111-120 
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Granular vesicles ; Ultrastructure ; Quantitative evaluation
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary In order to characterize different sub-zones in the palisade zone of the rat median eminence, quantitative ultrastructural parameters were applied to brains fixed in aldehyde-osmium tetroxide. The palisade zone has been subdivided in 4 successive sub-zones. Increasing numbers of granular vesicles (GV), especially those smaller than 110 nm, are observed from dorsal to ventral sub-zones. There also are more GV per unit area of nervous tissue in the perivascular than in the more dorsal sub-zones. The individual nerve profiles exhibit a larger size in the perivascular layer than in the more dorsal areas, whereas the number and size of nerve profiles devoid of vesicles diminish from dorsal to ventral. As a consequence more GV occur in the perivascular nerve profiles. In the GV containing nerve profiles the concentration of GV is, however, constant in the different sub-zones. A fluctuating size of the preterminal and terminal parts of the nerve fibres is suggested.
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  • 126
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    Cell & tissue research 136 (1973), S. 177-182 
    ISSN: 1432-0878
    Keywords: Rat ; Epididymis ; Effect of vasectomy ; Spermatozoa ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary Epididymal biopsies from rats that had undergone unilateral or bilateral vasectomies from one to eight months previously were compared with biopsies from their contralateral side or from normal controls to ascertain what ultrastructural changes had occurred. After vasectomy, spermatozoa appeared to dissolve in the lumen of the caput epididymidis and to be absorbed by the principal cells. About 5 weeks after vasectomy, numerous lamellar accumulations became apparent in the supernuclear region. Their resemblance to lysosomes or residual bodies was confirmed by an acid phosphatase reaction. After 10 weeks, similar lamellar and polymorphic accumulations on the contralateral side of animals with unilateral vasectomies indicated that resorption had also increased on the unligated side.
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  • 127
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    Cell & tissue research 138 (1973), S. 223-237 
    ISSN: 1432-0878
    Keywords: Aorta, endothelium ; Rat ; Transport of serum lipoproteins ; Radioautography ; Electron microscopy
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    Notes: Summary The transport of 125I-labeled serum lipoproteins through the aortic endothelium was studied by radioautography. Rat aorta and heart was perfused in vitro with a medium containing human very low density (VLDL), low density (LDL), high density lipoprotein (HDL), delipidated HDL apolipoprotein or rat HDL. In all lipoproteins more than 95% of the radioactivity was TCA precipitable and lipid radioactivity was from 2–4% in HDL, 4–6% in LDL, 7–15% in VLDL. After 18–60 min of perfusion and wash with unlabeled medium, most of the aortic radioactivity was TCA precipitable and the percent of lipid counts was similar to that in the original lipoprotein. Following perfusion with VLDL, LDL, or HDL the radioautographic reaction was seen over the endothelium, the subendothelial space and the inner media, and was separated by an unlabeled zone from the reaction present over the adventitia. Uniform labeling of the entire wall was found after perfusion with HDL apolipoprotein. The presence of silver grains over endothelial cells in regions rich in plasmalemmal vesicles suggested that these organelles participate in the transport of the labeled lipoprotein, as was shown for lactoperoxidase (Stein and Stein, 1972). The present data indicate that cholesterol may enter the aortic wall as a constituent of lipoprotein particles. Since an HDL particle carries less than 1/20 of the cholesterol present in a LDL particle, it seems that the lower susceptibility of the female to atheromatosis might be related to the higher ratio of HDL to LDL particles in the female serum.
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  • 128
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    Cell & tissue research 137 (1973), S. 141-143 
    ISSN: 1432-0878
    Keywords: Juxtaglomerular cells ; Rat ; Granulation index ; Diurnal rhythm ; Statistical evaluation
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    Description / Table of Contents: Zusammenfassung Das Granulations-Index der juxtaglomerulären epitheloiden Zellen der Rattenniere unterliegt tagesrhythmischen Schwankungen, wobei die berechneten Werte in den Nachtstunden significant höher sind als in den Tagesstunden.
    Notes: Summary Marked diurnal changes occur in the granulation index of the juxtaglomerular cells in the rat kidney. According to the results the night values are significantly higher than those obtained in daytime.
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  • 129
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    Cell & tissue research 140 (1973), S. 309-314 
    ISSN: 1432-0878
    Keywords: Pituitary Gland ; Rat ; Somatotropin ; Cellular Response ; Ultrastructure
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    Notes: Summary The response of somatotrophs to large doses of purified bovine growth hormone was examined in uninephrectomized rats drinking 1% sodium chloride in an attempt to prolong the action of the hormone. Growth hormone produced a triphasic response in the ultrastructure of somatotrophs. Uninephrectomy and salt alone induced no changes in somatotrophs as compared to unoperated animals consuming tap water. In the first phase (3 days) the cross sectional area of the cell increased, the size of the largest granule per cell as well as the number of granules per cell increased significantly. All these parameters then reached control level after 7 days of injections during a second phase. Suppression of somatotrophs by exogenous growth hormone was evident by 16 days (third phase) when the area of the cell, the size of the largest secretory granule as well as the number of granules per cell all decreased significantly. Somatotrophs apparently did not react to suppression by a lysosomal mechanism inasmuch as no increase in the number of lysosome-derived structures was seen even at 16 days.
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  • 130
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    Keywords: Vomeronasal organ ; Membrane particles ; Cell contacts ; Freeze-etching ; Rat
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    Notes: Summary The free surfaces and cell contacts in the epithelia of the vomeronasal organ of the rat were investigated by freeze-etching. The microvilli of receptor cells show a lower density of intramembranous particles (IMP) than the microvilli in the receptor-free epithelium. The ratio between the IMP on P and E-face is approximately 11∶1 in the receptor terminals, and 3.5∶1 in the cilia and microvilli of the receptor-free epithelium. Although atypical in length and only poorly equipped with rootlet fibers, the cilia of the receptor-free epithelium are furnished with typical ciliary necklace structures of up to 10 rows of membrane particles. Differences in the density of IMP on the P-faces of different cilia are probably due to continual ciliogenesis and also due to the different age of cilia in the receptor-free epithelium. Zonulae occludentes show different configurations in the neuroepithelium and in the receptor-free epithelium. In the former, they show a tendency to cross-link and form facet-like patterns, reflecting a constant morphology and relative stability for this apical region. In the receptor-free epithelium the junctional rows of zonulae occludentes display only loosely interconnected networks and a tendency to orient parallel to each other and to the free surface. In addition to zonulae occludentes, typical square aggregations of IMP are observed in the receptor-free epithelium. They are not exclusively restricted to the zone of intensive cell contacts by means of fine interdigitating cell processes, and their function has yet to be identified experimentally.
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  • 131
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    Cell & tissue research 239 (1985), S. 131-136 
    ISSN: 1432-0878
    Keywords: Capillary permeability ; Lanthanum ; Peripheral nerves ; Tooth pulp ; Ultrastructure ; Rat
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    Notes: Summary Experiments were performed to compare the permeability of capillaries supplying the endoneurial environment, which is invested by perineurium, with vascular permeability in the pulp where perineurium is absent. Anaesthetised rats were perfused through the aorta with physiological solutions containing lanthanum nitrate at 37° C. Pieces of inferior alveolar nerve and segments of mandibular incisors were immersion-fixed and transverse sections were examined electron microscopically for the distribution of lanthanum. In the pulp the nerve fibres pass between lanthanum-impermeable arterioles and venules en route to the incisal end. In the peripheral pulp a few capillaries were permeable but the most permeable capillaries lay between the odontoblasts. Pulpal capillary permeability was attributed to the fenestrated endothelium and contrasted with the unfenestrated endoneurial capillaries which were impermeable to lanthanum. Whereas the tight junctions of endoneurial capillaries are known to prevent certain blood-borne substances from entering the endoneurium, it was not clear whether the permeability of the pulpal capillaries, which are distant from the nerve fibres, could affect the nerve fibre environment. No extravasated lanthanum reached the pulpal nerve fibres suggesting that they are not affected. Technically it was not possible to examine the incisal third of the tooth where the situation could be different because the volume of the pulp decreases and capillaries lie closer to the nerve fibres.
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  • 132
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    Keywords: Neuropeptides ; Autonomic denervation ; Neuropeptide Y ; Vasoactive intestinal polypeptide (VIP) ; Substance P ; Urinary bladder ; Rat
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    Notes: Summary The origin and distribution in the urinary bladder of nerve fibers containing neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP) and substance P (SP) were investigated in rats. Experimental procedures comprised preganglionic decentralization or postganglionic denervation of the bladder and also chemical sympathectomy as well as capsaicin treatment of newborn rats. Nerve fibers containing NPY were richly distributed in the detrusor muscle and also in the pelvic ganglia. Numerous NPY-containing nerve cell bodies were found in pelvic ganglia. A rich occurrence of VIP fibers and a more sparse distribution of SP-containing fibers were also found in the bladder as well as a relatively rich representation of VIP- containing nerve cell bodies in the pelvic ganglia. After decentralization the intensity of VIP and NPY immunofluorescence increased in nerve cell bodies of the pelvic ganglia and in nerve fibers in the wall of the bladder. Postganglionic denervation, on the other hand, eliminated all peptides examined in the bladder wall. After postganglionic denervation the situation in the ganglia was approximately the same as after decentralization. Chemical sympathectomy (6-OHDA) did not seem to change significantly the frequency and distribution of VIP-, SP- and NPY-fibers in the muscle layer of the bladder or in the pelvic ganglia, while the NPY-containing nerve fibers in the submucosal layer and around blood vessels of the bladder disappeared. Adrenergic nerve fibers in the wall of the bladder (visualized by histofluorescence) were markedly reduced in number after administration of 6-OHDA. Capsaicin-treatment of newborn rats caused a loss of SP-fibers in the wall of the bladder, supporting the view that these fibers are sensory in nature in the urinary bladder. Although it cannot be entirely excluded that NPY-containing fibers in the wall of the bladder are adrenergic, the present results suggest that the NPY-fibers as well as the VIP-fibers of the bladder wall originate mainly in non-adrenergic cell bodies of the pelvic ganglia. However, perivascular NPY-containing nerve fibers are adrenergic in nature.
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    Cell & tissue research 239 (1985), S. 177-182 
    ISSN: 1432-0878
    Keywords: Lymphoid tissue ; Lung ; Bronchi ; Immuno-histochemistry ; Enzyme histochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunohistochemical, enzyme-histochemical and electron-microscopical methods were used to study non-lymphoid cells of control and stimulated rat bronchus associated lymphoid tissue (BALT) in situ and in suspensions. Particular attention was paid to the so-called antigenhandling cells, i.e., the interdigitating cells (IDC), which are situated in the T-cell areas, the follicular dendritic cells (FDC), which appear to be restricted to germinal centers, and macrophages, present both in T-cell and B-cell areas. The interdigitating cells were distinguished by being Ia-positive and by the presence of acid phosphatase and non-specific esterase activity in an area near the nucleus. Follicular dendritic cells could be observed in situ by using a monoclonal antibody and by the in vitro trapping of HRP-anti-HRP complexes. Several types of macrophages were found. At the electron-microscopical level no well-developed IDC and FDC could be detected in control BALT. However, in BALT of lipopolysaccharide-stimulated and mycoplasma-infected rats, well-developed IDC and FDC were found. It can be concluded that IDC's and FDC's can be found in BALT.
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  • 134
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    Cell & tissue research 239 (1985), S. 405-415 
    ISSN: 1432-0878
    Keywords: Testis ; Leydig cell ; FSH ; Morphometry ; Ultrastructure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of FSH on the testicular interstitial tissue of immature hypophysectomized rats were studied by comparing morphological changes in Leydig cells with quantitative changes in interstitial tissue histology using morphometric analysis. Three groups of rats received subcutaneous injections of 0.5 ml saline vehicle or 10 μg rFSH or 20 ng oLH (equivalent to the amount of LH known to contaminate the FSH), twice daily for 7 days. Administration of FSH significantly increased testis weight and stimulated more advanced spermatogenesis compared to saline or LH. Morphometric analysis of testes of LH-treated rats showed a small but significant increase in total interstitial cell volume compared to saline treatment. FSH caused much greater increases in the total volume of interstitial tissue and interstitial cells than either saline or LH and significantly increased the total volume of interstitial fluid by comparison with the other groups. FSH but not saline or LH treatment resulted in a striking hypertrophy of Leydig cells, to produce cells ultrastructurally identical to Leydig cells from adults. Since the target tissue of FSH is the seminiferous epithelium, the observed effects on Leydig cells by FSH treatment suggest that the secretion of factors by the seminiferous tubules may mediate the maturation of Leydig cells.
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  • 135
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    Keywords: Trachea ; Basal lamina ; Anchoring fibril ; Ruthenium red-positive filaments ; New anchoring device ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structural relationship between the basal lamina and the underlying reticular tissue was studied, with special attention to the relationship among basal lamina-associated anchoring fibrillar (AF) arcs (Kawanami et al. 1978, 1979) and other fibrillar components, in the epithelium-denuded trachea of the rat. Quantitative analysis of a large number of AF arcs reveals that the majority of the AF arcs has no other fibrillar components of passage. This suggests that most AF arcs do not serve as a real anchoring device, connecting the basal lamina with the underlying reticular tissue, as has so far been suggested by Kawanami et al. (1978). Ruthenium-red staining reveals the presence of a unique meshwork of microthread-like filaments connecting the undersurface of the basal lamina or the AF arcs with the underlying fibrillar components with a remarkable continuity, suggesting that the filaments act as a real anchoring device; these filaments link, instead of the AF arcs, the basal lamina, to the subjacent reticular tissue. Various enzymatic treatments of the filaments indicate that their chemical nature is probably non-collagenous (glyco)protein without glycosaminoglycan moieties.
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  • 136
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    Keywords: Key words Subcommissural organ ; Isograft ; Xenograft ; Reissner’s fiber ; Cerebrospinal fluid ; Rat ; Bovine
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    Notes: Abstract  The subcommissural organ (SCO) secretes glycoproteins into the cerebrospinal fluid (CSF) that aggregate and form Reissner’s fiber (RF). The factors involved in this aggregation are not known. One factor may be the hydrodynamics of the CSF when flowing through the aqueduct. This hypothesis was tested by isografting rat SCO and xenografting bovine SCO into the lateral ventricle of rats. Xenografts were either fresh bovine SCO or explants cultured for 30 days before transplantation. The grafts were investigated by electron microscopy and immunocytochemistry using antibodies against RF glycoproteins, serotonin and the glucose transporter I. Maximal time of transplantation was 43 days for isografts and 14 days for xenografts. The isografts were not reinnervated but were revascularized; they secreted into the ventricle RF glycoproteins that became progressively packed into pre-RF and RF structures identical to those formed by the SCO in situ. RF was confined to the host ventricle and at its distal end the constituent proteins disassembled. Xenografts were neither reinnervated nor revascularized and secreted into the host ventricle a material that never formed an RF. These findings indicate that the CSF factor responsible for the formation of RF is species specific, and that this process does not depend on the hydrodynamics of the CSF. The blood vessels revascularizing the isografted SCO acquired the characteristics of the vessels irrigating the SCO in situ, namely, a tight endothelium displaying glucose transporter I, and a perivascular space containing long-spacing collagen, thus indicating that basal release of glycoproteins may also occur in the grafted SCO.
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    Cell & tissue research 143 (1973), S. 143-147 
    ISSN: 1432-0878
    Keywords: Rat ; Suprachiasmatic nucleus ; Blinding ; Retinohypothalamic connections ; Karyometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Radioaktiv markierte Aminosäuren werden bei Säugetieren nach intraokulärer Injektion nicht nur zu den primären optischen Kerngebieten, sondern auch in den Nucleus suprachiasmaticus des Hypothalamus transportiert (Moore und Lenn, 1972; Hendrickson, Wagoner und Cowan, 1972; Moore, 1973). Dieser Befund macht eine direkte Verknüpfung zwischen der Netzhaut und dem sekretorisch aktiven Nucleus suprachiasmaticus wahrscheinlich, obwohl mit Silbertechniken eine solche Verbindung nicht sicher nachgewiesen werden konnte. Karyometrische Studien am Nucleus suprachiasmaticus männlicher Wistar-Ratten zeigen 6 Tage nach beidseitiger Blendung eine signifikante Abnahme der Zellkerndurchmesser. Dieses Ergebnis spricht für eine Aktivitätsänderung der Neurone im Nucleus suprachiasmaticus geblendeter Ratten.
    Notes: Summary Intraocular injection of labeled amino acids results, in different mammals, in an accumulation of radioactive material not only in the primary optic centers but also within the hypothalamic suprachiasmatic nucleus (Moore et al., 1972; Hendrickson et al., 1972; Moore, 1973). This finding argues in favour of a direct connection between the retina and the secretory suprachiasmatic nucleus although silver techniques do not show such a pathway. In male Wistar rats the nuclear diameter of the suprachiasmatic neurons decreases significantly 6 days after bilateral experimental ablation of the retina. These results indicate an alteration in activity of the suprachiasmatic neurons in blinded rats.
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    Cell & tissue research 143 (1973), S. 535-547 
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Rat ; Mitoses, topographic distribution ; Influence of fixation ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les mitoses de l'adénohypophyse du rat mâle ont été examinées comparativement après fixation par perfusion et après fixation par immersion. Après fixation par perfusion la répartition topographique des mitoses dans le parenchyme est homogène, et l'index mitotique est de 1,85 pour 1 000 cellules. Après fixation par immersion, la densité des mitoses décroit de la périphérie vers le centre, et l'index mitotique n'est que de 0,80 mitoses pour 1 000 cellules. La lenteur de pénétration du fixateur pendant l'immersion est tenue pour principal responsable des différences observées. Elle entraîne une modification de l'aspect des mitoses, telle que plus de la moitié d'entre elles ne sont pas identifiables et échappent aux comptages.
    Notes: Summary The mitoses of the adenohypophysis in male rats have been observed comparatively after fixation by perfusion and fixation by immersion. After fixation by perfusion, the topographic distribution of mitoses throughout the parenchyma is homogenous and the mitotic index is 1.85 for 1 000 cells. After fixation by immersion, the density of the mitoses is decreasing from the periphery towards the center and the mitotic index is only 0.80 for 1 000 cells. The slow penetration of the fixative during fixation by immersion is considered to be the main cause in the differences observed. This slow action causes a modification in the aspect of the mitoses, and more than fifty percent of them cannot be identified and escape to the counts.
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    Cell & tissue research 147 (1974), S. 259-269 
    ISSN: 1432-0878
    Keywords: Nerve endings ; Hair ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Palisade-shaped nerve endings of the small normal hairs of the rat snout were examined with the electron microscope (fixation by perfusion). The terminals are located inside the ‘glassy membrane’ in the area of the neck of the hair root. The 10–20 radially arranged terminal axons are in direct contact with the basement membrane of the epithelium of the external root sheath. The axons are surrounded on all sides by leaf-shaped processes of the Schwann cells. The surfaces of these cell processes are marked by numerous vesicle-like invaginations (approx. 1000 Å dia.). Transverse sections from several areas of the palisadeshaped nerve endings are compared with longitudinal sections. In the upper area ‘empty’ vesicles (approx. 500–600 Å in diameter) occur, along with electron-dense vesicles (approx. 800–1100 Å in diameter); in the middle area, the axons are distended and contain accumulations of mitochondria.
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  • 140
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    Cell & tissue research 147 (1974), S. 183-190 
    ISSN: 1432-0878
    Keywords: Kidney ; Rat ; Bowman's capsule ; Experimental hypertension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The parietal layer of Bowman's capsule was examined in the rat in control animals and in animals made hypertensive by treatment with desoxycorticosterone acetatesaline and by clipping of the renal artery. At an early stage of elevation of blood pressure and, occasionally, prior to elevation of blood pressure, the epithelial cells of this layer showed basal cytoplasmic protrusion and reduplication accompanied by an increase in the thickness of the basement membrane. These changes preceded evidence of glomerular or renal arteriolar pathology. They presumably reflect either a response of this layer to increased blood pressure and modified glomerular function, or a direct effect of the factors that also induces blood pressure elevation. In either case, they imply a greater functional significance for the parietal layer than has usually been attributed to it.
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    Cell & tissue research 148 (1974), S. 57-67 
    ISSN: 1432-0878
    Keywords: Prostate ; Rat ; Cyproterone ; Alterations ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary The dorsal lobe and the coagulating gland of the rat prostate were studied after administration of the anti-androgenic compound Cyp A for varying periods (7–20 days). There was a general involution of the organs with macroscopic atrophy and reduced amount of secretory material. Ultrastructurally the cells were pale, contained less organelles as compared with the controls, loss of cytoplasm and reduction both of cell height and width. The major changes also included reduction of the Golgi area, the rough endoplasmic reticulum and the number of free ribosomes. Within the dorsal lobe prominent nuclear changes were found, comprising different types of intranuclear inclusions of membranous configurations. The alterations observed in the present study are similar to the changes which were found in castrated animals and are consistent with atrophy. The results are discussed in relation to the available biochemical and autoradiographic data on the different lobes.
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  • 142
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    Cell & tissue research 125 (1972), S. 277-288 
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    Keywords: Neurohypophysis ; Rat ; Exocytosis ; Freeze-etching ; Ultrastructure
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    Notes: Summary Neural lobes of rats subjected to severe acute haemorrhage under sodium pentobarbitone anaesthesia were examined electron microscopically and the ultrastructure compared with that in anaesthetised and unanaesthetised controls. Changes in the localisation and numerical distribution of elementary granules and small vesicles in the neurohypophysial nerve endings of bled rats were consistent with the occurrence of exocytosis. The occurrence of “exocytotic profiles” was observed more frequently in freeze-etched tissue samples as compared with the material fixed for conventional electron microscopy. The ratio of small vesicles: elementary granules was shown to be significantly increased (P〈0.005) in the nerve endings of neural lobes from bled rats. Equally, the numbers of exocytotic profiles related to 1000 μm2 of neurohypophysial tissue area were significantly greater (P〈0.005) in bled rats.
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  • 143
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    Cell & tissue research 125 (1972), S. 289-294 
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    Keywords: Accessory optic system ; Rat ; Blood vessels ; Extracellular space ; Electron microscopy
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    Notes: Summary Two types of capillaries were found in the medial terminal nucleus of the rat accessory optic system. Type one capillaries are surrounded by glial processes and lack a perivascular space, whereas the type two capillaries and arterioles show a distinct extracellular perivascular space often filled with collagen fibrils. An internal as well as an external basal lamina lines these spaces. No fenestration of the endothelium was observed.
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  • 144
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    Cell & tissue research 125 (1972), S. 415-431 
    ISSN: 1432-0878
    Keywords: Synapses ; Rat ; Cerebral cortex ; Glutaraldehyde/E-PTA ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary Synaptic junctions in intact rat cerebral cortex have been examined following glutaraldehyde fixation and phosphotungstic acid (PTA) staining. In the presynaptic ending the network has a hexagonal arrangement, while the dense projections are regularly placed along the presynaptic membrane. Cleft densities occupy the intracleft region. The postsynaptic thickening extends uninterrupted along the length of the junction. Qualitatively, the majority of junctions fall into the ‘discontinuous-continuous’ category, in which the internal coat of the presynaptic membrane together with its associated dense projections is discontinuous along the length of the junction, whereas the postsynaptic thickening is continuous. By contrast, a small number of junctions are ‘continuous-continuous’. In an attempt to analyze the junctions quantitatively, nine indices were measured. Histograms of the size distributions of seven of these appear to be bimodal, and from this it is concluded that two junction populations may be distinguishable on quantitative grounds. It is also shown that the distance separating dense projections at the presynaptic membrane is of the order of 10–15 nm. This surprisingly low value has consequences for current ideas on the relationship between synaptic vesicles and dense projections, and these are discussed at length.
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  • 145
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    Cell & tissue research 125 (1972), S. 404-414 
    ISSN: 1432-0878
    Keywords: Renal medulla ; Rat ; Interstitial ground substance ; Fixation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Interstitium des inneren Nierenmarkes von Ratten wurde nach Anwendung verschiedener Fixierungsarten und Versuchsanordnungen elektronenmikroskopisch untersucht. Neben Bindegewebszellen und kollagenen Fibrillen enthält der interstitielle Raum bandförmige, basalmembranartige Strukturen, in deren weiträumigen Maschen zwei weitere Komponenten, nämlich ein feingranuläres und feinfilamentäres Material sowie kontrastreiche, globuläre Partikel liegen. Diese beiden Formelemente stellen sich bei den verschiedenen Fixierungsarten und Versuchsanordnungen unterschiedlich dar. Bei der feingranulären und feinfilamentären Komponente handelt es sich wahrscheinlich um interstitielles Blutplasma. Diese Deutung wird gestützt durch die morphologische Ähnlichkeit mit dem Blutplasma der Blutgefäße, die unterschiedliche Darstellbarkeit nach Glutardialdehyd-und Formalinfixierung sowie durch die Befunde nach Gefäßperfusion und Nierenvenenstauung. Das Verhalten der globulären Partikel nach OsO4-Einwirkung steht in Einklang mit der schon früher gegebenen Interpretation als präzipitierte saure Mukopolysaccharide. Ihr elektronenoptisches Bild wird im Vergleich zur bekannten makromolekularen Struktur der Mukopolysaccharid-Protein-Moleküle diskutiert.
    Notes: Summary The interstitial ground substance in the inner medulla of the rat kidney is examined electron microscopically after fixation with various fixation agents and under different experimental conditions. The papillary interstitium contains besides interstitial cells and few collagen fibrils basement membrane like material and two other components, fine granular-fibrillar elements and globular particles with heavy contrast. The appearance of the two latter interstitial components varies according to the different fixation agents used and to the experimental conditions. The fine granular and fine fibrillar material surrounds the globular particles numerously after glutardialdehyde-fixation but scarcely after formaldehyde-fixation. It increases after renal vein occlusion, but is diminished or absent, if the kidney is rinsed bloodfree. These results allow to conclude that this material represents interstitial bloodplasma. The behaviour of the globular particles after OsO4-fixation agrees well with the earlier interpretation according to which they represent precipitated acid mucopolysaccharides. Their electron microscopic appearance is discussed in comparison with the known macromolecular structure of the acid mucopolysaccharides.
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  • 146
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    Cell & tissue research 125 (1972), S. 432-447 
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    Keywords: Synaptosomes ; Synapses ; Rat ; Cerebral cortex ; Glutaraldehyde/E-PTA
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary Working with glutaraldehyde fixed, PTA stained rat cerebral cortex, the authors compared the ultrastructural features of synaptosomes with those of intact synaptic junctions. In general there is close correspondence between the two, although the cleft densities and postsynaptic focal densities of synaptosomes show a greater degree of focalization than their counterparts in synaptic junctions. The dense projections have similar profiles in both preparations, but are more difficult to distinguish clearly in synaptosomes on account of the closer packing of the presynaptic network around their apices. The limiting membrane of the presynaptic terminal is usually visible in synaptosomes, but not in synaptic junctions. Comparing the preparations quantitatively reinforces the qualitative findings, and points to their overall similarity. However a number of the indices in synaptosomes are significantly smaller than the corresponding ones in synaptic junctions, and this points to the operation of a shrinkage factor during fractionation procedures. This is confined to the pre- and post-synaptic components and does not affect the intervening contact region. Histograms of the size distributions of the indices are similar to those obtained for intact synaptic junctions, the majority displaying two peaks. It is concluded that synaptosomes accurately reproduce the major ultrastructural features of synaptic junctions.
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  • 147
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    Cell & tissue research 125 (1972), S. 480-496 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Normal ; Hypophysectomy ; Structural alteration
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    Notes: Summary The adrenal cortex of normal and hypophysectomized rats was observed by electron microscopy 5–90 days after the operation and, chiefly, the degeneration mechanism of the cortical tissue was discussed. In normal rats mitochondrial inner structures are generally tubular in shape in glomerulosa cells, vesicular (and tubulo-vesicular) in fasciculate cells and tubulo-vesicular (and vesicular) in reticularis cells. Some mitochondria in a few glomerulosa cells show long straight tubular crests in normal as well as hypophysectomized rats. Usually lipid droplets in normal glomerulosa, fasciculate and reticularis cells are surrounded by elements of smooth endoplasmic reticulum, which are very closely associated with mitochondria. About 10–25 days after hypophysectomy, most mitochondria in fasciculate and reticularis cells show tubular crests, and numerous electron lucent lipid droplets are accumulated in these cells. These droplets occupy most parts of the cytoplasm and are sometimes in contact with one another or with mitochondria. The elements of smooth endoplasmic reticulum and Golgi apparatus are markedly reduced in number and size. About 10–90 days after the hypophysectomy, the degenerative cortical cells containing irregularly shaped nuclei, heterogeneously dense bodies in various sizes, and a large number of electron lucent lipid droplets are seen. The author speculates that most fasciculate and reticularis cells degenerate because of a disorder in the lipid metabolism, since ACTH release, which is necessary for synthesis of corticosteroid hormones from cholesterol, is absent in hypophysectomy. The fatty degeneration of the fasciculate and reticularis cells is considered to be a main cause for attenuation of the cortical tissue. Sometimes atrophied cortical cells without lipid droplets are also found 60–90 days after hypophysectomy.
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  • 148
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    Cell & tissue research 144 (1973), S. 167-178 
    ISSN: 1432-0878
    Keywords: Prostate ; Rat ; Castration ; Alterations ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary The ventral, lateral and dorsal lobes of the rat prostate were studied 2, 3, 5, 7 and 21 days after castration. The major changes were a general reduction of the Golgi area and the RER. The cells became pale and contained less organelles compared with the normals. One of the most conspicuous changes were enormous autophagic vacuoles which were present already after 2 days, seemed to reach a numerical peak 3 days after castration and were seldom encountered after 7 days. Increased number of dense bodies were found within the same period. Lipid droplets were observed in the basal cytoplasm of the epithelial cells, especially in the ventral lobe. The basal cells showed the same involution as in the ordinary epithelium. In addition, macrophages were relatively numerous, and between the epithelial cells also lymphocytes were found. The results are discussed in relation to biochemical data on the different lobes.
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  • 149
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    Cell & tissue research 144 (1973), S. 179-189 
    ISSN: 1432-0878
    Keywords: Seminal vesicles ; Coagulating Gland ; Rat ; Castration ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The seminal vesicles and the coagulating gland of the rat were studied 2, 3, 5, 7 and 21 days after castration. The major changes within the seminal vesicles were primarily formation of whorls of the rough endoplasmic reticulum (RER), followed by a general atrophy with a numerical reduction of the RER-profiles, and with general simplification of the cytoplasm due to loss of the organelles. It was a gradually reduction of secretion granules, diminution of the Golgi apparatus, formation of pigment bodies and autophagic vacuoles. Lipid droplets were observed in the basal cytoplasm of the epithelial cells. In the coagulating gland, similar changes occurred within the Golgi area and the lysosome complex. On the other hand, cisternae of the basal endoplasmic reticulum tended to persist in many cells. The similarity in response strongly suggests that the pathogenetic mechanisms are similar in both organs, i.e. atrophy due to deprivation of the androgenic stimulus. The deprivation of androgen gave rise to an inflammatory-like process with infiltration of lymphocytes and macrophages. The increased number of macrophages may indicate that they contribute in some way to the involution of the prostate by removing the material in the autophagic vacuoles.
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  • 150
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    Cell & tissue research 150 (1974), S. 95-105 
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    Keywords: Mucosal mast cells ; Rat ; Compound 48/80 ; Degranulation, Release ; Light microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary Mast cells in the tongue and lamina propria of the duodenal mucosa, representing two cell types with different morphological, histochemical and functional properties have been studied under the electron microscope. Both cell types were found to contain similar moderately electron dense and homogeneous granules surrounded by a tight membrane constituting the basic ultrastructural characteristics of mast cells. The mucosal mast cells, however, contained fewer granules per cell and the individual granules often exhibited considerable variation in size. In addition the mucosal mast cells, unlike those of the tongue, peritoneum and skin, had a smooth plasma membrane lacking microvilli and possessed irregularly shaped or lobulated nuclei. In the tongue mast cells, administration of Compound 48/80 caused the formation of vacuoles around the granules, expulsion of granules from the cells and disintegration of the granular matrix, but no changes that could be attributed to 48/80 could be found in the mucosal mast cells.
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  • 151
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    Cell & tissue research 163 (1975), S. 249-262 
    ISSN: 1432-0878
    Keywords: Superior cervical ganglion ; Rat ; Catecholamines ; p-Chlorophenylalanine
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    Topics: Biology , Medicine
    Notes: Summary Ganglion cells and paraganglionic (PG) cells in the developing rat superior cervical ganglion were studied following postnatal treatment with p-chlorophenylalanine (pCPA) for 5 to 8 days. Litter mates, injected with saline solution, served as controls. Ganglion cells of control animals were differentiated ultrastructurally according to L. Eränkö (1972 a) into late sympathicoblasts and young sympathetic nerve cells. In both maturation stages treatment with pCPA caused marked swelling of mitochondria, concomitant with minor changes of other cell organelles. Parallel to the ultrastructural alterations, fluorescence microscopy and cytophotometry revealed a slight diminution of diffuse fluorescence intensity in sympathetic neurons as the expression of a mainly extragranular amine depletion. In distinction from ganglion blocking agents the alterations are regarded as a general toxic effect of pCPA upon maturing sympathetic neurons, which secondarily influences catecholamine storage sites. Following treatment with pCPA, in PG-cells an alteration of mitochondria was scarcely to recognize. Specific granules were distinctly decreased in number, in some cases to an almost complete degree. Concordant to ultrastructural observations a marked diminution of fluorescence intensity was demonstrable in SIF-cells. In addition in these elements the fluorescence spectrum shifted towards the green field. Fluorescence cytophotometric evaluations confirmed the optical impression. Provided, that PG-cells, demonstrated with electron microscopy, are identical with SIF-cells in fluorescence microscopy, the results are discussed on the basis of a specific decrease of primary catecholamines due to an enzyme inhibition involved in catecholamine synthesis.
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  • 152
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    Cell & tissue research 154 (1974), S. 443-470 
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    Keywords: Oocytes and T-prospermatogonia ; Rat ; Quantitative analysis ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bei der Ratte findet die sexuelle Differenzierung der Gonade zwischen dem 14. und 15. Tag post conceptionem (p.c.) statt. Während dieser Zeit teilen sich die Oogonien und deren Parallelpopulation—die M-prospermatogonien (multiplying prospermatogonia) oder I-Gonocyten—sehr häufig. Um den 17. Tag p.c. tritt die letzte Generation der Oogonien bzw. der M-prospermatogonien in die Mitose. Die Mitosen bilden häufig “synchronisierte Gruppen”. Die postmitotischen Kerne ihrer Tochterzellen—der Oocyten und der T 1 prospermatogonien (primary transitional prospermatogonia) oder II-Gonocyten—sind klein. Ihr Chromatin ist in Form gröberer Schollen der Kernmembran angelagert. Auf diese Weise kommt das typische “krustenförmige” Aussehen bzw. die Ähnlichkeit mit den Prophasen der Oogonien und M-prospermatogonien zustande. Die Oocyten durchlaufen zunächst die G1-phase (etwa 10 Std Dauer) und treten dann—am Ende des Präleptotänstadiums—in die S-phase. Dann passieren sie die verschiedenen Stadien der meiotischen Prophase und treten vom 3. Tag post partum (p.p.) ab in das Dictyotänstadium. Die T1-prospermatogonien hingegen befinden sich etwa 10 Tage lang in der G1-phase, ohne wesentliche morphologische Veränderungen aufzuweisen. Vom 4. Tag p.p. an durchlaufen sie die S-Phase. Die S-Phasen-Dauer (D-S) beider Zellarten beträgt 11.5 Std und wurde durch Doppelmarkierung mit 14C- und 3H-Thymidin bestimmt. Als am besten geeignete Termine für die Bestimmung der D-S erwiesen sich bei den Oocyten der 18. Tag p.c. und bei den T1-prospermatogonien der 5. Tag p.p. Zu diesen beiden Zeitpunkten war die Anzahl der in die S-phase ein-und austretenden Oocyten bzw. T1-prospermatogonien gleich, die Zellen in S-phase befanden sich im “steady state”. Das Kernvolumen der Oogonien und M-prospermatogonien ist etwa doppelt so groß wie das der postmitotischen Oocyten und T1-prospermatogonien. Bis zum 5. Tage p.p. nimmt das Kernvolumen der Oocyten und T1-prospermatogonien um etwa das Fünffache zu. Der Degenerationsindex der Oocyten liegt wesentlich höher als der der T1-prospermatogonien; er ist postnatal besonders hoch. Aus den T1-prospermatogonien gehen am 4. und 5. Tage p.p. durch Teilung die T 2-prospermatogonien (secondary transitional prospermatogonia) hervor. Die Kerne dieses Zelltyps sind etwas kleiner also die der T1-prospermatogonien. Die T2-prospermatogonien treten am 6. Tage p.p. in die Mitose; es entstehen die ersten A-spermatogonien.
    Notes: Summary In the rat (Wistar-WU) sexual differentiation of the gonads occurs between days 14 and 15 post conception (p.c.). At this time the oogonia and their parallel population — the M-prospermatogonia (I-gonocytes)—divide rapidly. On about day 17 p.c., the last generation of oogonia and M-prospermatogonia, frequently arranged in synchronized clusters, enters mitosis. The postmitotic nuclei of their daughter cells—oocytes and T 1-prospermatogonia (II-gonocytes)—are small; coarse flakes of chromatin are associated with the nuclear membrane causing the typical “crustlike” appearance and the similarity with the prophases of oogonia and M-prospermatogonia. After the oocytes have passed a G1-phase of approximately 10 hr, they enter the S-phase at the end of the preleptotene stage. Then they pass the different stages of the meiotic prophase until they enter the dictyate stage from 3 day post partum (p.p.) onwards. The T1-prospermatogonia, on the other hand, spend a long G1-phase of about 10 days without any conspicuous morphological change before entering the S-phase from day 4 p.p. onwards. The duration of the S-pbase (D-S) of both cell types—oocytes and T1-prospermatogonia—as determined by the double labeling method with 14C- and 3H-thymidine is found to be 11.5 hr. The most favourable time for determining the D-S was day 18 p.c. for the oocytes and day 5 p.p. for the T1-prospermatogonia. On these two days the balance was reached between the cells entering and leaving the S-phase. The nuclear volumes of the postmitotic oocytes and T1-prospermatogonia are approximately half the size of those of their precursors. Until day 5 p.p. the nuclear volumes of the oocytes and T1-prospermatogonia increase about fivefold. The degeneration index of the oocytes is considerably higher than that of the T1-prospermatogonia; postnatally it is especially high. T 2 prospermatogonia arise by mitosis of the T1-prospermatogonia on day 4 and 5 p.p. The nuclei of this cell type are smaller than those of T1-prospermatogonia. T2-prospermatogonia enter mitosis on day 6 p.p and give rise to A-spermatogonia.
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    Cell & tissue research 155 (1974), S. 231-243 
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    Keywords: Pinealocyte-resembling cells ; Habenular region ; Sympathetic nerve fibres ; Ontogenetic development ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cells of the pineal gland, the pineal stalk, and the lamina intercalaris contain 5-HT and are innervated by sympathetic nerve fibres. These peripheral nerve fibres continue rostrally from the lamina intercalaris and run into the central nervous tissue of stria medullaris and the habenular nuclei. Pharmacological treatment to increase the cellular 5-HT content revealed that the sympathetic fibres are in close relation to yellow fluorescent cells embedded in the brain tissue. These yellow fluorescent cells develop very late in the ontogenetic development (three weeks or more postnatally) and are preceded by ingrowth of sympathetic fibres into the brain tissue. The results support the hypothesis that the cells found in the habenular region are of pinealocyte rather than neuronal nature, but it is possible that they differ in certain aspects from the cells of the pineal gland proper.
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    Cell & tissue research 170 (1976), S. 43-60 
    ISSN: 1432-0878
    Keywords: Interdigitating cells ; Lymph node ; Rat ; Ultrastructure ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electronmicroscopic and cytochemical studies were performed to localize interdigitating reticulum cells (IDC) in the popliteal lymph node of the rat. The morphological features of the IDC of the rat correspond to those described for other species, but also show similarities to normal macrophages in the rat. This is considered to be an argument in favour of the common origin of IDC's and macrophages. Ultrahistochemical studies with horseradish peroxidase (HRP) reveal no phagocytotic capacity of IDC's. After perfusion fixation containing ruthenium red (RR) the surface coat stains heavily: RR is also found deep in the membrane invaginations of the IDC, indicating the presence of polyanionic sialoglyco-proteins. The post-capillary-venules (PVC) are very permeable to both HRP and RR. The phosphotungstic acid-chromic acid stain (PTA-CrA) also reveals glycoproteins in the surface coat; these glycoproteins are susceptible to α-neuraminidase, whereas glycoproteins in the Golgi complexes, lysosomes and in the vesicular complexes of IDC are not. The glycoproteins of the latter are susceptible to 0.1 N NaOH. These findings indicate that IDC produce different kinds of glycoprotein, one of which may be secreted and act as a factor for stimulating peripheral T-lymphocytes. Intimate contact between IDC's and PCV's could be observed. It is therefore conceivable that IDC's play an important role in the homing of T-lymphocytes.
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    Cell & tissue research 161 (1975), S. 183-191 
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Pituitary ; Rat ; Localization of 3H-dexamethasone ; Radioautography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The central nervous system and the pituitary of adrenalectomized male rats injected with 3H-dexamethasone were examined by radioautography. At 1 hr after the injection, radioactivity concentration was high in the medial basal hypothalamus, the pituitary and the pineal gland. In the hypothalamus, radioactive material was found to be selectively concentrated in neurons in the ventral part of nucleus arcuatus and in the infundibular region. In the anterior pituitary, a large proportion of cells showed silver grains both in the cytoplasm and over the cell nuclei. However, in a small number of cells, the radioactive material was associated with the cell nuclei. Less radioactivity was present in the intermediate and posterior lobes. The pineal gland contained more silver grains than did other regions of the brain. The results obtained in the present study suggest essentially an action of dexamethasone in the medial basal hypothalamus and at the level of the pituitary.
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  • 156
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    Keywords: Median eminence ; Rat ; Monoamine ; ZIO reaction ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary The reaction of nerve endings in the median eminence of the rat to zinc iodide-osmium tetroxide (ZIO) staining was examined electron microscopically under normal and experimental conditions. The experimental condition of catecholamine exhaustion in the nerve endings was induced by the administration of H44/68 and reserpine. Vesicles in the terminals of catecholaminergic nerves reacted similarly to ZIO staining in both normal and experimental material. The majority of synaptic vesicles in various terminals gave a positive ZIO reaction. The neurosecretory elementary granules, however, failed to react with ZIO. On the other hand, some nerve terminals in the external layer of the median eminence showed a strong positive reaction in the cytoplasmic matrix, in mitochondria as well as in synaptic vesicles. These findings strongly suggest that the ZIO-positive substance in nerve terminals is not the transmitter itself, i.e. the monoamine, but rather represents a range of substances commonly found in various kinds of synaptic vesicles and is probably proteinaceous in nature. A brief discussion is also given on the difference in ZIO reactivity between neurosecretory elementary granules and small vesicles in the hypothalamo-hypophyseal tract.
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  • 157
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    Cell & tissue research 166 (1976), S. 407-412 
    ISSN: 1432-0878
    Keywords: Pituitary primordium ; Cytodifferentiation ; Rat ; Organ culture ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rathke's pouches isolated from rat fetuses on day 12 were maintained in organ culture for 9 days and investigated immunohistochemically to test whether or not the hypothalamus is involved in the cytodifferentiation of the adenohypophysis. The unlabeled antibody enzyme method demonstrated that the cultured tissue contains different types of glandular cells, i.e., adrenocorticotropin (ACTH)-, growth hormone (GH)-, luteinizing hormone (LH)-, thyrotropin (TSH)-, and prolactin-producing cells. Indirect evidence was also obtained to indicate the presence of melanocyte stimulating hormone (MSH)-cells. These findings suggest that adenohypophysial primordial cells of rats start to synthesize their respective hormones without stimuli from neurosecretory substances of the brain which are known to be essential for the maintenance of the secretory activity of the adult gland.
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  • 158
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    Keywords: Microtubules ; Tractus hypophyseus ; Diabetes insipidus ; Rat
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary Evidence has already been adduced suggesting that an increase in microtubular number occurs in the tractus hypophyseus of rats stressed by the administration of hypertonic saline, and of rats with congenital diabetes insipidus (CDI). Since the tractus hypophyseus in these animals shows high secretory activity, it seems likely that the microtubular increase reflects the participation of microtubules in axoplasmic transport. To exclude, however, a congenital microtubular abnormality in CDI, affected newborn rats were examined. In these, the microtubular number was normal, thus suggesting that the increase in microtubular number seen in adult animals was not a congenital morphological abnormality. However, by 4 days of age there was a slight but statistically significant increase in microtubular number in affected rats, a change probably attributable to increased secretory activity.
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  • 159
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    Keywords: Neurohypophysis ; Rat ; Vasopressin release ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Le lobe postérieur de l'hypophyse a été examiné sur coupes ultrafines chez 45 rats (24 normaux, 17 déshydratés respectivement 1, 2 et 4 jours, et 4 anesthésiés à l'éther), après fixation au glutaraldéhyde ou selon la méthode de Karnovsky, post-fixation osmiée et double contraste à l'acétate d'uranyle et au citrate de plomb. Des fibres neurosécrétoires sombres et claires sont reconnaissables, mais les données manquent encore pour interpréter cette différence. Des densifications juxta-membranaires sont observées dans les terminaisons neurosécrétoires là où se trouvent des amas de microvésicules. Des fibres neurosécrétoires traversent parfois des pituicytes; il est aussi souvent observé des fibres, chargées de granules, libres dans un espace péricapillaire. Les espaces péricapillaires, ramifiés en tous sens loin des vaisseaux, développent une large surface de contact avec les extrémités nerveuses et les prolongements de pituicytes. Dans des conditions techniques bien contrôlées, la déshydratation n'entraîne pas de modifications appréciables des granules de neurosécrétat après 24 h. Ensuite les granules sont diminués en nombre, de façon très considérable le 4e jour; mais les granules restants ont un contenu dense normal; jamais il n'a été observé d'aspects de ≪granules vides ≫. Après anesthésie prolongée à l'éther, il n'y a aucune modification visible ni du nombre, ni de la densité des granules. Ces observations sont discutées quant au mécanisme de l'excrétion de vasopressine; elles sont en faveur de l'existence de deux pools hormonaux, l'un libre et rapidement disponible, l'autre plus fortement lié et certainement contenu dans les granules jouant le rôle de réserve. Toutefois un mécanisme d'exocytose granulaire ne peut être formellement exclu.
    Notes: Summary Hypophysial neural lobes of 45 rats (24 controls, 17 dehydrated resp. 1, 2 and 4 days, and 4 ether anesthetized) were fixed either with glutaraldehyde or according to Karnovsky and post-fixed in osmium tetroxyde; ultrathin sections were stained by uranyl acetate and lead citrate. Dark and clear neurosecretory fibres were observed, but sufficient data are still lacking for a valuable interpretation of these aspects. Juxta-membraneous densifications are visible in limited areas of neurosecretory terminals where clusters of microvesicles are present. Neurosecretory fibres happen to be completely encircled by pituioyte cytoplasm; fibres loaded with elementary granules are frequently encountered running free in a pericapillary space. Pericapillary spaces stretch out branching far away from vessels, resulting in a widespread contact with nerve terminals and pituicyte processes. In accurately controlled technical conditions, dehydratation does not result in any noticeable change of neurosecretory granules after 24 h. A decrease of the number of granules follows and is extremely conspicuous after 4 days; though, remaining granules keep a normal dense content, and aspects of “empty granules” have never been observed. After prolonged ether anesthesia, no visible change either in number or electron density of granules was observed. These findings are discussed in consideration of the mechanism of vasopressin release; they support the hypothesis of two hormonal pools, one of which would be free and rapidly available for release, the other being more tightly bound and certainly located in granules representing a storage site. Though granular exocytosis cannot be absolutely excluded.
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    Cell & tissue research 109 (1970), S. 384-397 
    ISSN: 1432-0878
    Keywords: Heart muscle cells ; Electron microscopy ; Quantitative ; Asphyxia ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Normale und hypoxische Herzmuskelzellen aus der Wand des linken Ventrikels der Ratte wurden quantitativ-morphologisch anhand von elektronenmikroskopischen Längsschnitten nach Perfusionsfixierung untersucht. In normalen Zellen waren alle Myofibrillen relaxiert, die mittlere Sarcomerlänge betrug 2,2 μm. Die Schnittfläche wurde zu 55% von Myofibrillen, zu 27% von Mitochondrien und zu 18% von Grundplasma und Reticulum eingenommen. Die zwischen den Myofibrillen liegenden Mitochondrien waren längsoval und im Mittel 2,3mal so lang wie breit. Es bestand kein Unterschied zwischen subendokardial und subepikardial gelegenen Zellen. 10 min nach Erstickung der Tiere waren in den sonst unauffälligen Muskelzellen die Glycogengranula vermindert. Nach 20 min führte die Hypoxie zu einer Zunahme der relativen Schnittfläche der Mitochondrien um etwa 16% und zu einer beginnenden Kontraktur der Myofibrillen (Sarcomerlänge 2,0 μm). 20 min Hypoxie in Hypothermie (25–30°C intrathorakal) veränderte die normale Zellstruktur dagegen kaum. Wenn die Herzen während der 20 min dauernden Hypoxie in Normothermie mit einer procainhaltigen sauerstoff- und glucosefreien Blutersatzlösung durchspült wurden, waren die Myofibrillen relaxiert, die Schwellung der Mitochondrien dagegen wurde nicht reduziert. 30 min nach Erstickung wurde die Kontraktur stärker (Sarcomerlänge 1,7 μm). Nach 60 min bildeten sich Superkontraktionsknoten, einzelne Myofibrillen waren in Höhe der I-Bänder unterbrochen. Die Cristae der Mitochondrien wichen auseinander, die Schnittfläche der Mitochondrien hatte um 27% zugenommen. Während in Normotherapie eine Asphyxie des Tieres bereits nach 10 min die Herzmuskelzellen funktionell schwer schädigt, ist die Schädigung morphologisch erst nach 20 min eindeutig. Das bedeutet, daß für die elektronenmikroskopische Präparation eine Hypoxie von unter 10 min bedeutungslos ist. Hinsichtlich der morphologischen Manifestationszeit für die Unterbrechung der Sauerstoffversorgung stimmen unsere Befunde an Herzmuskelzellen gut mit vergleichbaren Angaben an Leberzellen überein.
    Notes: Summary In heart muscle cells of the left ventricle of rats the distribution of cell organelles and their reaction to hypoxia were investigated by electron microscopy. In normal hearts fixed by perfusion with aldehydes, the mean sarcomere length was 2.2 μm. 27% of the longitudinal sectional area was occupied by mitochondria, 55% by myofibrils and 18% by sarcoplasmic reticulum and ground plasm. The mitochondria situated in rows between the fibrils were oval and measured 2.3 times more in length than in width. There was no difference between cells from subendocardial and subepicardial regions. 10 min hypoxia (complete occlusion of the trachea) did not affect the appearance of muscle cells but diminished the number of glycogen granules. After 20 minutes the area occupied by mitochondria was increased by 16%, the mitochondria between the myofibrils were more spherical and only 1.5 times longer than wide. The sarcomeres shortened to 2.0 μm. With hypothermia (25–30°C) hypoxia of 20 minutes duration did not affect the cell structure. Perfusion of the heart by a saline solution, which contained procaine but neither oxygen nor glucose, for 20 minutes prevented shortening of the sarcomeres but not swelling of the mitochondria. 30 minutes after occlusion of the trachea the myofibrils shortened to a sarcomere length of 1.7 μm. After 60 minutes irregularly and excessively contracted myofibrils appeared and some sarcomeres were interrupted at the level of the I-bands. In some of the swollen mitochondria the cristae were widely separated. The increase of the area occupied by mitochondria was 27%. Asphyxia affects heart muscle cells severely with respect to function within 10 min, but morphologically it takes 20 min before a definite effect can be noticed. As to the time after which lack of oxygen is manifested morphologically, our results are consistent with findings in liver cells.
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  • 161
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    Cell & tissue research 110 (1970), S. 540-558 
    ISSN: 1432-0878
    Keywords: Cerebral cortex ; Synapses ; Rat ; Postnatal differentiation ; Morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of layers I and II of the motor cerebral cortex of rat brain has been studied at birth, 4, 7 and 14 days postnatal and in the adult. Compared with the adult, neonatal rat motor cortex exhibited a large extracellular space which decreases with increasing age. At all stages studied the neurons were seen to contain the organelles usually found in adult neurons. Growth cones were present in decreasing numbers up to 14 days old. Synapses were detectable at birth and there was an obvious increase in their number throughout the postnatal development. At the earliest stages studied there was a lack of specialization characteristic of the adult. Many synapses were either avesicular or relatively so and lacked the high degree of modification of adult pre- and postsynaptic membranes. By 7 days after birth many synapses existed which in all morphological respects resembled those of the adult, and by 14 days, the majority were of the adult type. These findings, particularly with reference to the postnatal development of synapses, have been discussed in relation to the known electrophysiological findings.
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  • 162
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    Keywords: Ependyma ; Thiobarbiturate narcosis ; Cell nucleus shrinkage ; Rat
    Source: Springer Online Journal Archives 1860-2000
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    Description / Table of Contents: Zusammenfassung Im Ependym des Hypothalamus wurde das Verhalten der Zellkern-Membran nach ausgedehnter intraperitonealer Thiobarbiturat-Narkose untersucht. Die Kernmembran ist gefaltet, der Zellkern zeigt Zeichen von Schrumpfung. Diese Veränderung ist reversibel und wird nicht von der mit der Narkose verbundenen Entwässerung verursacht. Elektronenmikroskopische Untersuchungen lassen einen normalen Stoffwechsel vermuten, Zeichen von Pyknose fehlen.
    Notes: Summary Effects on the nuclear membrane were investigated in hypothalamic ependymal cells of the rat during prolonged intraperitoneal thiobarbiturate narcosis. The nuclear membrane of the nucleus was folded and the nucleus showed signs of shrinkage. These alterations were reversible and did not depend on the dehydration which developed during the narcosis. Ultrastructural signs and the absence of pyknosis suggested that metabolism during nuclear shrinkage may be normal.
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  • 163
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    Cell & tissue research 113 (1971), S. 157-173 
    ISSN: 1432-0878
    Keywords: Prostate ; Development ; Ultrastructure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The postnatal development of the rat prostate has been studied with the electron microscope. Major developmental changes begin during the second week after birth and involve organelles associated with the formation of secretions. The amount of granular endoplasmic reticulum and the size of the Golgi complex increase greatly. Large vacuoles that probably contain secretory material are formed, and the lumen of the prostatic acini appears to contain secreted material. Large lysosomes with polymorphic interiors are present as early as 10 days after birth, and they become numerous by the end of the third week. Differences in fine structure between the different lobes of the prostate are detectable in 10–14 day old rats. The subsequent differentiation of the granular endoplasmic reticulum into the forms characteristic of the different prostatic lobes is described. The initial changes in the prostate occur in advance of sexual maturity of the animal, and the adult appearance of the gland is attained by 4–5 weeks after birth.
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  • 164
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    Keywords: Neurosecretory cells ; Rat ; Cytoplasmic bodies resembling nucleoli ; Paranucleolar and coiled bodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A distinctive cytoplasmic inclusion, termed nucleolus-resembling body, was identified in the neurons, but not in the glial cells, of the medial vascular prechiasmatic gland, the preoptic recess and the anterior part of the suprachiasmatic area in the rat. This ultrastructure which lacks a limiting membrane is made of an entanglement of tightly packed filaments, 60–70 Å in diameter. Each filament consists of smaller units, 15–20 Å thick. Usually, only one such ultrastructure is present in a cell, but at time two were identified. It is present in most neurosecretory cells. It is surrounded by polyribosomes in the perikaryon of the nerve cells. Furthermore, it is linked to peripherally located ribosomes by means of filaments, 70 Å in diameter. A possible function in protein synthesis of structural components, such as neurofilaments, is hypothesized. Two distinctive fibrillar areas were identified in the nucleolus according to the tightness with which fibrils were packed together. Their significance is discussed. Nucleolar extrusions were never observed in our studies. Paranucleolar and coiled bodies were identified in the nuclear sap. The mechanism of formation and the significance of these bodies are discussed.
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  • 165
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    Cell & tissue research 115 (1971), S. 30-45 
    ISSN: 1432-0878
    Keywords: Adrenal Cortex ; ACTH ; Autoradiography ; Stereology ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The changes occurring in rat adrenocortical cells (zona fasciculata) during an 8 day period of treatment with ACTH, were investigated by morphometric and autoradiographic methods. The most important ultrastructural change consists in a conspicuous increase in the smooth endoplasmic reticulum, that accounts for about 50% of the total increase of cellular volume. Also the mitochondrial fraction shows a significant increase, which is found to be due both to the increment in the number of mitochondria per cell and to the increase in the mean volume of organelles themselves. The quantitative autoradiographic data, indicating an increment in the incorporation of 3H-orotate and 3H-leucine into adrenocortical cells of the treated animals, allow us to conclude that the ACTH-induced ultrastructural changes are the morphological expression of a stimulation of the cellular protein synthesis. Since mitochondria are largely autonomous in the synthesis of their enzymes and structural proteins, it is possible to hypothesize that ACTH also intervenes in the regulation of the mitochondrial protein synthesis.
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  • 166
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    Keywords: Adenohypophysis ; Pars intermedia ; Corticotrophic cells ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Nos observations démontrent l'existence de deux types de cellules glandulaires dans la pars intermedia de l'hypophyse du rat: des cellules propres, à M.S.H., dont les grains de sécrétion sont détruits par la fixation osmiée et relativement bien conservés par la fixation au glutaraldéhyde; des cellules à petits grains denses, osmio-résistants, qui s'apparentent morphologiquement aux éléments corticotropes de l'antéhypophyse. L'existence bien connue d'A.C.T.H. ou, plus précisément, d'un facteur ≪A.C.T.H.-like≫ dans le lobe neuro-intermédiaire nous fait considérer comme très vraisemblable la nature corticotrope de ce deuxième type cellulaire de la pars intermedia.
    Notes: Summary Two types of glandular cells have been shown to occur in the intermediate lobe of the rat hypophysis: 1) M.S.H. producing cells whose secretory granules are destroyed by osmium fixation and relatively well preserved by glutaraldehyde fixation, 2) cells with small dense osmium resistant granules being morphologically similar to the corticotrophic cells of the anterior lobe. The well known occurrence of A.C.T.H. or, more precisely, of an “A.C.T.H.-like” factor in the neuro-intermediate lobe makes the corticotrophic nature of this second cell type very likely.
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  • 167
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    Cell & tissue research 117 (1971), S. 191-211 
    ISSN: 1432-0878
    Keywords: Sensory neurons ; Rat ; Nerve section ; Neurofilaments ; Neurofibrils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sensory neurons were examined in spinal ganglia of the rat 1 to 55 days after section of the plexus brachialis nerves. Only “light” neurons of the type A were investigated. Maximal reaction to axotomy was found 7 to 14 days after the operation. The majority of the axotomized perikarya developed central chromatolysis. In such neurons, Nissl bodies virtually disappeared from the central area of the neuron and formed a more or less continuous zone at the cell circumference. The cytocentrum became filled with large numbers of mitochondria, dense bodies and other organelles. Neurofilaments and microtubules were disarranged and ran at random among the accumulated particles. Microtubules were often more prominent in chromatolytic areas than neurofilaments. Both these organelles were rare in the peripheral areas filled with massed Nissl substance. Part of the neurons that did not show typical chromatolysis contained increased numbers of neurofilaments among Nissl bodies dispersed throughout the cytoplasm. Neurofilaments were roughly arrayed in bundles up to several microns wide; they were linked by cross-bridges and separated by distances of about 500 Å. Microtubules were rarely found in the filamentous areas. However, they were numerous in the axon hillock and in the initial segment where they formed fascicles similar to those described in normal neurons of other types. During the period from 14 to 55 days after axotomy, many perikarya recovering from chromatolysis contained enlarged bundles of neurofilaments with occasional microtubules among the restored Nissl bodies. Mean diameters of sensory neurons, measured 7 to 55 days after axotomy, in no instance exceeded those of contralateral control neurons. It thus appears that sensory perikarya do not increase in size either during the chromatolytic process or during the period of recovery.
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    Cell & tissue research 116 (1971), S. 136-150 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex difference ; ACTH ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Nebennierenrinde von 178 Sprague-Dawley-Ratten wurde elektronenmikroskopisch von der 1.–12. Lebenswoche unter Normalbedingungen sowie nach s.c. Injektion von 3 IE ACTH/100 g Körpergewicht innerhalb von 2 min — 24 Std untersucht. Ab der 5. Lebenswoche unterscheiden sich die Nebennieren von Weibchen im Bereich der äußeren Zona fasciculata durch Kernvergrößerung, größere Mitochondrien und kleindisperse Liposomen von denen der Männchen. Zyklusbedingte Schwankungen in der Ultrastruktur der weiblichen Nebennierenrinde bestehen nicht. Nach ACTH-Applikation nähert sich das Nebennierenbild des Männchen innerhalb von 30 min dem des unbehandelten Weibchens. Außerdem kommt es bei beiden Geschlechtern durch ACTH zu einer Dispersion und Reduktion der Liposomen, Vergrößerung des Golgiapparates und der Zellkerne, Ausweitung des endoplasmatischen Retikulums und Vermehrung der Mikrovilli. Der Geschlechtsdimorphismus wird funktionell erklärt.
    Notes: Summary 178 Sprague-Dawley-rats were studied by electron microscopy from the 1st to the 12th week of life under normal conditions and after s. c. injection of 3 I. U. ACTH/100 gbodyweight from 2 min to 24 hours. Beginning with the 5th week of life females differ from males by small dispersed liposomes and enlarged nuclei and mitochondria in the outer z. fasciculata. There is no change in the ultrastructure of the female adrenal cortex depending on the sexual cycle. After application of ACTH the adrenal cortex of males assimilates to the untreated females within 30 min. After ACTH-application both sexes show dispersion and reduction of liposomes, dilation of endoplasmatic reticulum, increasement of microvilli and enlargement of the Golgi apparatus. The sex-dimorphism is interpreted by functional differences.
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  • 169
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    Cell & tissue research 119 (1971), S. 378-383 
    ISSN: 1432-0878
    Keywords: Seminal vesicles ; Rat ; Epithelium ; Proliferation activity ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung An 12 männlichen weißen Wistarratten unterschiedlichen Alters wurde autoradiographisch die Proliferation des Samenblasenepithels zur Zeit der Pubertät geprüft. Die Tiere waren 21, 29, 40, 50, 60 und 180 Tage alt und erhielten 1 Std vor der Tötung durch Dekapitation 2,5 μC 3H-Thymidin/g Körpergewicht i. p. appliziert. Ausgewertet wurden Autoradiogramme nach einer Expositionszeit von 18 Tagen; bestimmt wurde der Prozentsatz der markierten Zellkerne (3H-Index). Im Gegensatz zu dem bekannten exponentiellen Abfall des 3H-Indexes bei nicht-hormonabhängigen Leberepithelien jugendlicher Ratten kommt es beim Epithel der Samenblasen um den 40. Lebenstag zu einem erneuten, starken Proliferationsschub. Diese starke Wachstumszunahme ist wahrscheinlich der proliferationskinetische Ausdruck der nach Steinberger (1970) zur gleichen Zeit stattfindenden hormonellen Umschaltung in den Testes. Anschließend geht das Vermehrungswachstum des jugendlichen Tieres, durch das die Zellzahl erhöht wird, in das Erhaltungswachstum über; der 3H-Index des Samenblasenepithels erreicht den Wert des erwachsenen Tieres.
    Notes: Summary In 12 male white rats (Wistar) the proliferative activity of the epithelial cells in the seminal vesicles was studied by autoradiography. The animals were 21, 29, 40, 50, 60 and 180 days old. Each animal was given 2.5 μC/g 3H-thymidine and was killed 1 hour thereafter. We analysed the autoradiograms recording the percentage of labelled nuclei (3H-index). While the 3H-index of the non hormone-dependent epithelial cells in the liver of young rats declines continously with growing age (Stocker et al., 1964), the epithelial cells in the seminal vesicles of 40 days old rats show a remarkable rise in their proliferative activity. It is quite possible that this new peak in the proliferation is the morphological result of a preceeding switch in hormonal production of the testes as reported by Steinberger (1970). Thereafter the growth proliferation of the young rats declines into the steady state of the grown up.
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    Cell & tissue research 119 (1971), S. 334-354 
    ISSN: 1432-0878
    Keywords: Fat absorption ; Small intestine ; Rat ; Micropinocytosis ; Circadian rhythm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die vorliegende Untersuchung will klären, welche Rolle die Mikropinocytose bei der Fettresorption spielt und ob die Fettresorption tagesrhythmische Schwankungen aufweist. Zu diesem Zweck wurde Fett zu verschiedenen Tages- und Nachtzeiten in abgebundene Jejunumabschnitte von Ratten injiziert, das Darmgewebe in verschiedenen Abständen licht- und elektronenmikroskopisch untersucht und die Zahl der mikropinocytotischen Invaginationen der Enterocyten bestimmt. Folgende Resultate wurden erzielt: Die Häufigkeit der Mikropinocytose (mikropinocytotische Invaginationen/100 Mikrovilli) beträgt bei Hungertieren 4,78, bei Normaltieren 5,06, 1 min nach Gabe von Mazolachymus 3,55, 1/16 m NaCl-Lösung 4,37, Butterschmalz 5,50, Rindertalg 7,16, Schweineschmalz 7,66 und Mazola 9,25; 2 1/2 min nach Mazolagabe 8,23, nach 5 min 7,67, nach 10 min 6,76. Zwischen 10 und 15 min wird der Ausgangswert erreicht. Aufgrund statistischer Auswertung der Befunde wird geschlossen, daß Fett vor allem in den Frühstadien der Fettresorption durch Mikropinocytose aufgenommen werden könnte. Ferner zeigt sich, daß die Mikropinocytosehäufigkeit entscheidend von der chemischen Zusammensetzung und dem physikalischchemischen Zustand der verabreichten Fette beeinflußt werden dürfte. — Lichtmikroskopisch wurde nachgewiesen, daß die Fettresorption charakteristische, tagesrhythmische Schwankungen aufweist. Um 2.00 Uhr ist sie am stärksten, um 14.00 Uhr am geringsten. Es wird diskutiert, worauf die tagesrhythmischen Unterschiede zurückzuführen sind.
    Notes: Summary The present study attempts to elucidate (a) to what extent micropinocytosis is involved in the process of fat absorption and (b) whether fat absorption shows a circadian rhythm. For this purpose rats were given 0.1 to 0.15 ml of fat directly into the upper jejunum at various times of the day and night. 20 sec to 20 min after administration of fat small pieces of the jejunum were rapidly dissected out, the tissue examined by means of light- and electron microscopy, and the number of micropinocytotic pits occurring in the enterocytes during fat absorption was determined. The following results were obtained: The frequency of micropinocytosis (micropinocytotic pits/100 microvilli) is as follows: in starved rats 4.78, in normal rats 5.06, 1 min after administration of corn oil chyme 3.55, 1/16 m NaCl-solution 4.37, clarified butter 5.50, ox tallow 7.16, lard 7.66, corn oil 9.25; 2 1/2 min after administration of corn oil 8.23, after 5 min 7.67, and after 10 min 6.76. Between 10 and 15 min the number of micropinocytotic pits reverses to normal. From statistical analysis of the results it is concluded that micropinocytosis can play a significant role in the early stages of fat absorption. The experiments show in addition that micropinocytosis is greatly influenced by the chemical composition and the physico-chemical state of the administered fat.—Light microscopic studies indicate that fat absorption varies considerably during the 24 hr period. The highest absorption was found at 2 a.m., the lowest at 2 p.m.
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    Cell & tissue research 124 (1972), S. 1-11 
    ISSN: 1432-0878
    Keywords: Spermiogenesis ; Rat ; Spermatids ; Manchette ; Microtubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The manchette or caudal tube has been examined in Stage 14 rat spermatids. The microtubules of the caudal tube have been found to be partially sheathed by smooth endoplasmic reticulum which appears to be continuous with the outer nuclear membrane of the redundant nuclear envelope. The microtubules in caudal regions of the manchette have been noted to be interconnected by links of unusual size and morphology. It is suggested that the caudal tube consists at this stage of development of two structures, membrane and microtubules and that the links between the microtubules appear to play a role in the structural order noted in the position of the tubules of the manchette. The possible significance of these links in relation to motility is discussed.
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    Cell & tissue research 124 (1972), S. 311-319 
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Neurovascular contacts ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the median eminence of the rat axons of the supraoptic-paraventricular-hypophyseal tract with elementary neurosecretory granules (150–200 mμ) traverse the internal zone. Terminals containing dense core vesicles 60–120 mμ in diameter end on the portal capillaries of the median eminence. A unique organisation of the primary portal capillaries is shown. Endothelial cells have many fenestrae. The pericapillary space has numerous extensions all of which represent a special zone around the endothelial tube. The fine structural organisation and function of the neurovascular contacts in the median eminence are discussed.
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    Cell & tissue research 126 (1972), S. 41-52 
    ISSN: 1432-0878
    Keywords: Pituitary ; Rat ; Oestradiol influence ; STH-cells ; Leucine incorporation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pituitaries of male and female rats neonatally treated with oestradiol dipropionate were examined with light and electron microscopy, disc electrophoresis and in vitro C14-leucine incorporation. Single dose of oestradiol administered shortly after birth to male rats has an prolonged stimulative effect on LTH synthesis and release. This is expressed in the cell hypertrophy, proliferation of ER, increase of corresponding band density and higher rate of 14C-leucine incorporation. In females this effect was less pronounced. STH cell in the treated male pituitaries were degranulated, corresponding hormone band density significantly lowered and incorporation of 14C-leucine was reduced. This could be considered as an inhibitory effect of oestradiol on STH synthesis and release. Such effect was more expressed two than four months after treatment and is more pronounced in male than in female rats.
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    Cell & tissue research 126 (1972), S. 421-430 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Growth ; Sex-dimorphism ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Anhand des Verlaufs des 3H-Index und der Mitoserate in Anhängigkeit vom Lebensalter wurde die Proliferationsaktivität in den Zonen der Nebennierenrinde untersucht. 84 SPF-Ratten erhielten 2 μCi/g 3H-Thymidin i.p.; der Untersuchungszeitraum erstreckte sich vom 18. Trächtigkeitstag bis zur 12. Lebenswoche. Alle Zonen zeigten in der Rangfolge Glomerulosa — Fasciculata — Reticularis eine Abnahme DNS-synthetisierender Zellkerne. Geschlechtsspezifische Unterschiede im Ausmaß der Proliferationsaktivität konnten zu keiner Zeit nachgewiesen werden. Aus der Dissoziation der Kurven des 3H-Index und der Mitoserate bei vergleichbaren DNS-Syntheseraten wird auf eine Änderung der G2-Phase des Generationszyklus der NNR-Zellen in der teilexponentiellen Wachstumsphase geschlossen.
    Notes: Summary In 84 SPF-rats the poliferative activity of the adrenal cortical cells was studied from the 18th day of pregnancy up to 12 weeks post partum. Rats were given 2 μC/g tritiated thymidine and killed 1 hour thereafter. It was shown that there was no sex-related difference in the degree of proliferation that could explain the sexual dimorphism in adrenal weights. In all cortical zones a decrease in the number of labeled cells was seen during the obvservation period. The highest percentage of labeled cells was found in the glomerulosa. There exists no parallelism of the mitotic and labeling index, which gives evidence for a change in length of the G2-phase of the cell generation cycle.
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  • 175
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    Cell & tissue research 127 (1972), S. 483-491 
    ISSN: 1432-0878
    Keywords: Lateral preoptic area ; Hypothalamus ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The arcuate nucleus, median eminence, and the lateral preoptic area from the brains of aldehyde-perfused male and female rats were examined by electron microscopy. In the lateral preoptic area, three neuronal types are described: a small light neuron, a larger light one, and a dark neuron resembling the larger light one in size and nuclear shape. Many myelinated axons are interposed among single neurons or neuronal pairs. The relationship of structures to each other is discussed. Several observations not previously reported are illustrated from tissue of the arcuate nucleus and median eminence.
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  • 176
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    Cell & tissue research 124 (1972), S. 354-366 
    ISSN: 1432-0878
    Keywords: Hippocampal region ; Rat ; Monoamine oxidase ; Chemoarchitectonics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. The distribution of monoamine oxidase (MAO) in the hippocampal region of the rat has been studied in cryostat sections with the tryptamine-tetrazolium method of Glenner et al. 2. The staining pattern was rather diffuse as compared with the distribution of MAO in the guinea pig and of other enzymes in the rat hippocampal region. 3. However, there were differences in enzyme activity between different areas and layers which could be correlated to some degree with results obtained with other methods, e.g., silver impregnation, catecholamines, and acetylcholinesterase.
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  • 177
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    Cell & tissue research 128 (1972), S. 406-425 
    ISSN: 1432-0878
    Keywords: Parafollicular Cells ; Rat ; Normalcalcemia ; Vitamin D2 ; Electronmicroscopy ; Histochemical fluorescence method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Thyroid parafollicular cells of normocalcemic and vitamin D2-treated rats were investigated by electron microscopy and with the histochemical fluorescence technique of Hillarp and Falck. Administration of high doses of vitamin D2 caused hypercalcemia and an extensive degranulation of the parafollicular cells. The formation and storage of monoamines in granulated and degranulated parafollicular cells was investigated by fluorescence microscopy after injection of monoamine precursors (DOPA, 5-HTP), alone or in combination with Ro 4-4602, nialamide or reserpine. No fluorescence was observed in parafollicular cells of untreated rats. l-DOPA and l-5-HTP (but not the corresponding D-amino acids) were taken up by a process closely linked to the decarboxylation of the amino acids to the corresponding amines (dopamine and 5-hydroxytryptamine). Treatment with vitamin D2 did not seem to affect the formation of amines in the parafollicular cells or the formation and storage of amines in other cell systems investigated. The amine itself (dopamine) was not taken up by the parafollicular cells. In normocalcemic rats, the amine formed was retained in the cytoplasm of the parafollicular cells by a partially reserpine-resistant mechanism. The storage of amines is concluded to occur in association with the calcitonin-containing granules. In parafollicular cells of vitamin D2-treated rats, a certain amount of amine was bound in the cytoplasm in the absence of typical granules. As a considerable amount of calcitonin is known to remain in the thyroid of vitamin D2-treated rats, the present observations may indicate an association between the amine and the polypeptide hormone calcitonin, whether the latter is confined to typical granules or not.
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  • 178
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    Cell & tissue research 129 (1972), S. 471-499 
    ISSN: 1432-0878
    Keywords: Cardiac muscle cells ; Rat ; Mitotic division ; Myofibril changes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the myocyte at all phases of mitosis as well as of early postmitotic cells has been studied in the myocardia of 14- and 18-day rat embryos and 5- and 7-day old rats. The myofibrils remain unchanged up to the late prophase. In prometaphase the majority of Z-disks in embryo myocyte myofibrils and considerable part of these disks in myofibrils of suckling rats are drastically disintegrated. This is followed by a progressive isolation and scattering of the myofilament bundles and of the whole sarcomeres during the subsequent phases of mitosis. Thick myofilaments seem to be unchanged but thin ones become frequently poorly outlined (mainly in embryos). The sarcoplasmic reticulum, including its typically differentiated subsarcolemmal cisternae, exhibits relatively few changes during mitosis. In the early postmitotic period there is a gradual restoration of contrast-rich Z-bands, interconnecting the previously isolated sarcomeres. Patterns of this process have much in common with early stages of myofibrillogenesis (appearance of subsarcolemmal “Z-bodies”, formation of skeins of thin filaments etc.). The cleavage furrow formation is either absent or considerably retarded up to the postmitotic period. Behaviour of some other organelles during myocyte mitosis has been described. Possible mechanisms and significance of the observed phenomena are discussed.
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  • 179
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    Cell & tissue research 130 (1972), S. 58-69 
    ISSN: 1432-0878
    Keywords: Infundibulum ; Rat ; Ependymal and glial cells ; Inclusions ; Neuroglial synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Zahlreiche Ependymzellen des Recessus infundibularis und Gliazellen der Zona externa besitzen Fortsätze, die bis an den perivaskulären Raum der Kapillarschlingen des Portalplexus heranreichen. Die Ultrastruktur dieser Gefäßfortsätze wird beschrieben. Sie können verschiedenartige Einschlüsse enthalten: 1. große, runde osmiophile Einschlüsse (0,5–2 μ im Durchmesser), die als „lipid inclusions“ bezeichnet werden; 2. unregelmäßig geformte, granuläre Einschlüsse mit gleichmäßiger Elektronendichte (500 bis 2000 Å im Durchmesser); 3. rundliche Granula mit einem hellen Zentrum und ringartig um dieses Zentrum gelagerten elektronendichten Körnchen (1200–1600 Å im Durchmesser). Der letztgenannte Granulatyp scheint ein charakteristisches Merkmal der Gefäßfortsätze in der Zona externa der Wistar-Ratte zu sein. Meist häufen sich die Körnchen gemeinsam mit den anderen beschriebenen Einschlüssen in den kolbenförmigen Endigungen der Gefäßfortsätze. Exocytosevorgänge an der dem perivaskulären Raum zugewandten Oberflächenmembran der Fortsätze weisen auf eine Abgabe von Substanzen an die Blutbahn hin. Neurogliöse Synapsen finden sich im Infundibulum vorwiegend zwischen Nervenfasern des Tractus tuberohypophyseus und Gefäßfortsätzen der Ependym- und Gliazellen. Der synaptische Spalt enthält häufig fädige oder körnige Strukturen. Solche Partikel finden sich zusammen mit tubulären oder vesikulären Membranprofilen auch im synapsennahen Bereich des Glia- oder Ependymfortsatzes. Die funktionelle Bedeutung dieser Synapsen insbesondere für die Abgabe neuronaler Substanzen wird diskutiert.
    Notes: Summary Many ependymal cells of the infundibular recess and many glial cells of the external layer send their processes to the perivascular space of capillary loops of the portal plexus. The ultrastructure of these vascular processes is described. They may contain various inclusions: (1) large, osmiophilic globules (0,5–2 μ in diameter), mostly termed “lipid inclusions”; (2) irregularly formed, granular inclusions with an evenly distributed content (500–2000 Å in diameter); (3) circular granules comprising an electron-lucent centre surrounded by an annular wall of electron-dense particles (1200–1600 Å in diameter). This type of granule seems to be characteristic for the vascular processes in the external layer of the Wistar rat. Frequently accumulations of these granules are found together with other inclusions in the widened end-feet of the vascular processes. The occurrence of exocytosis at the perivascular surface membrane of the vascular processes indicates a release of substances into the blood. Neuro-glial synapses in the infundibulum are mainly found between nerve fibres of the tuberohypophyseal tract and the vascular end-feet of ependymal and glial cells. The synaptic cleft often contains filamentous or granular material. Together with tubular and vesicular membrane profiles such particles are also found in the postsynaptic area of the ependymal and glial processes. The functional significance of these contacts is discussed particularly with respect to the release of neuronal substances.
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    Cell & tissue research 129 (1972), S. 92-113 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Neurohypophysis ; Neurosecretory granule ; Rat ; Membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neurosecretory granules (NSG) of rat posterior pituitary glands were prepared by differential centrifugation techniques mainly according to the procedure as described by Barer, Heller and Lederis (1963). As revealed by electron microscopy, the recovery of neurophysin and the contents of enzymes, purified NSG were obtained in a pellet at 30 000 g/60 min (0.44 M sucrose). Eighteen h after injection of (35S) cysteine into the supraoptic nucleus 60% of the recovered radioactivity in the neural lobe was found in the NSG, whereas 20% was found in the final supernatant (100 000 g/120 min). Sixteen days after injection the NSG and the final supernatant fraction contained fairly equal amount of (35S) cysteine (approximately 40%). It is suggested that after a period of intragranular maturation neurophysin is extruded into an extragranular pool of neurosecretory material. With the use of conventional polyacrylamide-gel electrophoresis it was shown that the predominating proportion of radioactivity in the NSG after a hypothalamic injection of (35S) cysteine was located within the neurophysin fraction A and in fraction B. Fraction B is suggested to be partly bound to the NSG membranes. When the NSG soluble and NSG insoluble proteins, obtained after lysis of NSG, were separated on polyacrylamide gels in the presence of sodium dodecylsulphate, the highly radioactive soluble protein was shown to consist of two components with average molecular weights of 12 300 and 14 600. Most of the proteins in the lysate were found in the NSG membranes, though less radioactive. A component with a mol.wt. of 37 000 was enriched in the membrane fraction. At longer times after isotope injection the high mol.wt. proteins, particularly those of the NSG membranes, contained increased amounts of radioactivity.
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  • 181
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    Keywords: Kidney ; Rat ; Actin- and myosin-like filaments ; Tubule and interstitial cells ; Electron microscopy
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    Notes: Summary By electron microscopy, the prominent bundles of filaments occurring in the basal part of proximal and distal tubule cells and in interstitial cells of rat kidney cortex were studied in cells fixed by vascular perfusion, in glycerol-extracted cells and in glycerol-extracted cells treated with heavy meromyosin (HMM). The studies of perfusion-fixed tissue showed that the proximal tubule cells contained in their most basal part filamentous bundles oriented transversely around the tubule. The bundles consisted of trightly packed thin filaments (50–80 Å in diameter). Similar but less prominent bundles were found in distal tubule cells and in interstitial cells. The dimension of these filaments was similar to that of actin filaments and their insertion in the basal cell membrane of the tubule epithelial cells resembled the insertion of actin filaments in the cell membrane of smooth muscle cells. The studies on glycerol-extracted cells revealed that some tubule cells contained two types of filaments (60–80 Å and 130–170 Å in diameter) located side by side in the basal filamentous bundles. The dimension of the thick filaments corresponds well to the values for myosin filaments in glycerinated smooth and skeletal muscle. The studies on HMM-reacted renal tissue revealed that the thin filaments (60–80 Å) described in tubule and interstitial cells are probably actin filaments, as they formed characteristic arrowhead complexes morphologically indistinguishable from the complexes of HMM with actin filaments in smooth and striated muscle cells. Our results provide strong evidence that a two-filament contractile system, based on interaction of actin and myosin filaments, exists in renal tubule and interstitial cells. As a hypothesis it is proposed that it is changes in tonus of the basal filamentous system in the proximal tubule cells which stabilize the intratubular pressure, possibly via angiotensin.
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    Cell & tissue research 200 (1979), S. 163-177 
    ISSN: 1432-0878
    Keywords: Gastrin cells ; Entero-endocrine cells ; Rat ; Cell isolation ; Pylorus
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    Topics: Biology , Medicine
    Notes: Summary A technique has been developed to obtain viable, isolated and enriched populations of gastrin cells (G-cells) from the rat stomach. Restricted tissue samples from a small area of the pyloric antrum known to be particularly rich in G-cells, were sequentially digested with pronase followed by mechanical agitation, to remove the epithelial cells. This technique resulted in a significant enrichment of G-cells (3–4 fold) since the surface epithelial cells and upper portions of the glands were discarded before the initial G-cell fraction was collected. These cells in suspension were then isolated from each other by gentle pipetting in a DNase containing solution and designated the crude preparation (CP). The G-cells were then purified further by separating the cells according to size by velocity sedimentation. The greatest concentration of G-cells (15–25 %) was found in the fraction containing cells with diameters of 10 to 12 μm. The effectiveness of the technique was evaluated by counting G-cells as identified by electron microscopy and immunofluorescence and assessing gastrin activity by radioimmunoassay. All three methods indicated that cell separation by gravity velocity sedimentation enriched the G-cell population 15–20 fold over their concentration in the CP. The combined techniques of selective pronase digestion followed by gravity velocity sedimentation resulted in an isolated cell preparation containing a 50–100 fold increase of G-cells over their normal distribution in the intact gastric mucosa. Since these isolated G-cells retain features indicating viability, their usefulness for in vitro studies is suggested.
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  • 183
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    Cell & tissue research 140 (1973), S. 473-479 
    ISSN: 1432-0878
    Keywords: Testicular feminization ; Rat ; Leydig cells ; Sterility ; Androgens, Steroids ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary The interstitial cells of the pseudohermaphrodite rat testis are both hypertrophic and hyperplastic. The cytoplasm is characterized by smooth endoplasmic reticulum which is abundant and variable in form. Mitochondria are numerous and large with tubular cristae and occasional inclusions. Structural features of the Leydig cells indicate potential for increased steroid synthesis. The presence of large numbers of mast cells in the intertubular area is confirmed. Small seminiferous tubules lack advanced germinal elements. Additional connective tissue and myoepithelial layers produce a thickening of the limiting membrane. Some myoepithelial cells are atypical with an electron translucent cytoplasm and nuclei with dense peripheral chromatin. No spermatogenic cells beyond the cap phase of the spermatid are observed. The cytoplasm of Sertoli cells contains large lipid droplets and degenerating germ cells.
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    Cell & tissue research 239 (1985), S. 37-42 
    ISSN: 1432-0878
    Keywords: Subcapsular sinus ; Subsinus layer ; Peripheral cortex ; Lymph node ; Rat
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary The peripheral cortex of a lymph node consists of folliculo-nodules (follicles with a nodule or germinal center) and an extrafollicular zone. In the course of an analysis of the nodes in rats under various experimental or abnormal conditions, our attention became focused on the particularities of a thin layer of peripheral cortex underlying the subcapsular sinus. The present paper reports on observations which led to the identification of the “subsinus layer” of the peripheral cortex. The observations suggest that this layer complements the activity of the inner wall of the subcapsular sinus. The layer probably contributes to the selection of certain elements from the afferent lymph and guides them towards the node area where they can join the cell population involved in the immunological activity in which they are to participate.
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  • 185
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    Keywords: Pancreatic acini ; Secretion ; Amylase ; Secretagogues ; Receptors ; Rat
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    Notes: Summary Incubation of rat pancreatic lobules for 90 min with optimal concentrations of caerulein, carbachol or secretin caused the release of about 30% of the amylase content. Combination of secretin with carbachol or caerulein increased the amylase output to about 40%. With secretin, as with carbachol or caerulein, heterogeneity of cellular responsiveness was observed, some acini being partially or completely depleted of their zymogen granules, whereas others appeared to be resting. When secretin was combined with carbachol or caerulein, granule depletion, originally confined to small groups of neighbouring acini, spread to form large areas of degranulated cells, sometimes comprising a whole section of a lobule. In dispersed acini, under the same conditions, carbachol caused the release of about 60% of the amylase content, and secretin 40%. When both secretagogues were combined, a significant increase to 78% was observed. Under these conditions, there was some important cellular damage, as indicated by the release of 20% of the amylase content and between 6 and 12% of lactate dehydrogenase into the media, in the absence of stimulus. These results were corroborated by cytological observations. On the basis of their secretory response two groups of acini can be distinguished, those that respond to carbachol, caerulein or secretin and those that respond to the combination of secretin with carbachol or caerulein. Electrophoretic patterns of secretory proteins released by lobules stimulated by these different types of secretagogues were essentially similar. The pattern was quite different, however, in the absence of a stimulus. The most striking feature was the presence of a band at 63 Kd whereas a 73.5 Kd band was found only under conditions of stimulation. The latter results support the view that under resting and stimulated conditions secretory proteins are released from distinct compartments in the acinar cell.
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    Cell & tissue research 239 (1985), S. 229-233 
    ISSN: 1432-0878
    Keywords: Chemotherapy ; Vincristine ; Constipation ; Mucosa ; Regulatory peptides ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The microtubule-disrupting drug vincristine is a common component of anti-cancer chemotherapeutic regimes, which produces acute constipation as a side effect. Although generally attributed to damage to the myenteric plexus, the precise mechanism of this disturbance is unknown. In addition, vincristine causes marked aberrations in the secretory response of pancreatic endocrine tissue in both man and rats. No information is available on its possible effect on regulatory peptides of the gastrointestinal tract. In this study we have produced vincristine-induced constipation in rats at a dosage comparable with that employed in the treatment of human subjects. Immunocytochemistry revealed concomitant disturbances in cells exhibiting immunoreactivity for gastrin in the antrum, for gastric inhibitory polypeptide and 5-hydroxytryptamine in the duodenum, for enteroglucagon in the colon, and for somatostatin in all three sites. These widespread effects are transient in nature with normal cell numbers and morphology being reestablished within 6 days. It is suggested that the observed effects are a direct result of microtubule disruption and that gastrointestinal regulatory peptide and amine immunoreactive cells have a rapid regeneration potential.
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    Cell & tissue research 239 (1985), S. 235-239 
    ISSN: 1432-0878
    Keywords: Erythropoiesis ; Autophagy ; Mitochondria ; Electron microscopy ; Stereology ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Late erythroblasts and reticulocytes from bone marrow of male Wistar rats were studied by electron-microscopic stereology. Late erythroblasts with morphological signs of nuclear extrusion (EN+erythroblasts) and late erythroblasts without these signs (EN-erythroblasts) were analysed separately. The volumes of mitochondria, autophagosomes, autophagocytosed mitochondria, autophagocytosed cytoplasm and degraded material inside autophagosomes were calculated per unit volume of cytoplasm. The results demonstrate that (1) the volume density of mitochondria in the cytoplasm decreases by 34% during maturation from (EN-)- to (EN+)-erythroblasts (p〈 0.001) and by 60% during differentiation from (EN+)-erythroblasts to reticulocytes (p〈0.001), (2) a fivefold increase in the volume density of autophagosomes in the cytoplasm is noted during maturation from (EN-)- to (EN+)-erythroblasts (p〈0.01), whereas the value of this parameter remains essentially unchanged during the subsequent differentiation to reticulocytes, (3) no mitochondria are found inside autophagosomes of (EN-)-erythroblasts, whereas mitochondria occupy 26% and 35%, respectively, of the autophagosomal volume in (EN+)-erythroblasts and in reticulocytes. Our results show that autophagocytosis of mitochondria starts at the moment of nuclear extrusion and continues in the bone marrow reticulocytes.
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  • 188
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    Keywords: C cells ; Thyroid ; Rat ; Ultimobranchial origin ; Differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Nos observations confirment l'origine ultimobranchiale des cellules C de la thyroïde du rat. L'évolution des corps ultimobranchiaux (C.U.B.) a été étudiée à partir du 16ème jour de gestation, stade où ils fusionnent avec l'ébauche thyroïdienne. Dès le 17ème jour, les premières cellules C se différencient dans le C.U.B. inclus dans la thyroïde. Au 18ème jour, elles sont plus nombreuses et commencent à se disperser dans les cordons thyroïdiens. La migration des cellules C est particulièrement importante au 19ème jour. A partir du 20ème jour, les signes d'activité sécrétoire s'intensifient très nettement. A la fin de la vie foetale, les cellules C sont comparables aux cellules C adultes. La differenciation des cellules parathyroïdiennes précède nettement celle des cellules C.
    Notes: Summary Our observations confirm the ultimobranchial origin of the C cells of the rat thyroid. We studied the development of the ultimobranchial body from the 16th day of pregnancy, when there is fusion with the thyroidian anlage, onwards. As early as the 17th day, the first C cells differentiate in the ultimobranchial body which is included into the thyroid. On the 18th day, they are more numerous and start to scatter throughout the thyroidian cords. The migration of the C cells is especially obvious on the 19th day. From the 20th day onwards, there is marked increase in secretory activity. At the end of the foetal life, the C cells resemble those of the adult. The differentiation of the parathyroid cells significantly precedes that of the C cells.
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    Cell & tissue research 146 (1973), S. 463-471 
    ISSN: 1432-0878
    Keywords: Neurosecretory granule ; Pars nervosa ; Rat ; Morphometry
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    Notes: Summary The distribution of the diameters of the neurosecretory granules in the rat pars nervosa (measured from electron micrographs taken at 40 000 × ) was compared among axons by nonparametric statistical methods and the axons were classified into five groups with median granule diameters of 143, 155, 167, 180 and 193 nm. We suggested that these five axon types carried different secretory substances contained in the pars nervosa.
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    Cell & tissue research 148 (1974), S. 203-211 
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Rat ; Supraoptic nucleus ; Ultrastructure ; Somatic spines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopic studies of ultrathin serial sections of the perikarya and adjacent neuropil of neurons of the supraoptic nucleus (SON) of the adult male rat revealed varying forms of two types of somatic spines. One type forms synapses with axons passing the cell, the other, without synapses, appears to serve as a buttress or clasp for adjacent neuronal and glial processes. The synapse-bearing spines lack the usual spine apparatus but contain the flocculent substance often seen in spines. The other spines do not exhibit either of these structures. These somatic spines were also seen in Golgi impregnated preparations but the types could not be distinguished. Certain axons synapse either on a somatic spine of the perikarya or penetrate the glial sheath of the neuron and synapse, usually repeatedly, on the soma in an en passant manner.
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    Cell & tissue research 148 (1974), S. 371-379 
    ISSN: 1432-0878
    Keywords: Pineal gland ; Rat ; Electric coupling areas ; Freeze-etching
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    Topics: Biology , Medicine
    Notes: Summary The pineal glands of Wistar rats were fixed in glutaraldehyde and prepared by the freeze-etching technique. With regard to the form and internal structure of pineal cells this technique has revealed nothing essentially new. Synaptic ribbons are very rarely found. A regular diagonal arrangement of membrane attached particles between the Schwann cell and the axon of an autonomic nerve terminal was observed. Three types of contact areas between pineal cells are described. In the first two types, which occur more frequently, the diameter of the area varied from 1000 to 2500 Å, with irregularly aggregated membrane associated particles. The third type, larger but less frequently found, has a surface area of 0.4 μm2 and much more regularly distributed membrane associated particles (ø 88 Å). These contact areas can be considered to be electric coupling areas between pineal cells.
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    Cell & tissue research 158 (1975), S. 281-283 
    ISSN: 1432-0878
    Keywords: Glycocalyx ; Urinary bladder ; Ruthenium red ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Earlier statements to the contrary, the present study demonstrates the presence of a cell surface coat (glycocalyx) on the luminal plasma membrane of the super ficial transitional epithelial cells lining the urinary bladder of male Buffalo rats. This coat was demonstrated with ruthenium red, an electron dense stain, which revealed a surface layer, 60–80 Å thick, separated from the outer leaflet of the plasma membrane by an electron lucent layer, approximately 30 Å thick. The structure of the glycocalyx was not affected by 12 weeks of treatment with dibutylnitrosamine, a known bladder carcinogen.
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    Cell & tissue research 159 (1975), S. 49-62 
    ISSN: 1432-0878
    Keywords: Autonomic ganglia ; Pelvic plexus ; SIF cells ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To further evaluate the role of autonomic ganglia in the regulation of pelvic visceral activity, the neural elements in the major pelvic ganglion of the male rat have been studied with histochemical and electron microscopic techniques. The principal findings are that the ganglion is composed of cholinergic and adrenergic ganglion cells as well as small intensely fluorescent (SIF) cells. Polarity in the ganglion is indicated by clustering of small ganglion cells which stain intensely for acetylcholinesterase (AChE) along the pelvic nerve while larger cells, with weak to moderate AChE activity, collect near small branches of the hypogastric nerve. Some cholinergic ganglion cells are enclosed by a plexus of adrenergic terminals. SIF cells appear to be in contact with both cholinergic and adrenergic cells, although many of the fluorescent beads around adrenergic neurons may be short dendrites of ganglion cells, rather than processes of SIF cells. Two types of SIF cells may be distinguished on the basis of size and morphology of their granulated vesicles. Afferent synapses of the cholinergic type were common on SIF cells of the large granule and small granule type. Portions of SIF cells with large granules occur within the capsule of ganglion cells. Contacts seen here were interpreted as efferent synapses from SIF cells to the dendrites of ganglion cells.
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    Cell & tissue research 171 (1976), S. 375-379 
    ISSN: 1432-0878
    Keywords: Corpus luteum ; Autografts ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The rat ovary has been transplanted successfully to subcutaneous tissue areas by several investigators. Light microscopy has revealed that corpora lutea in ovarian autografts are formed by luteinization of intact follicles and contain entrapped ova. In the present study, corpora lutea from autografted ovaries in castrate rats were obtained at metestrus and examined electron microscopically to determine whether their cellular morphology correlated with the normal progesterone levels in these animals. Cellular features usually accepted as regressive were apparent. The findings suggest either structural luteolysis is occurring before functional luteolysis or that the adrenal has increased steroidogenic activity in the castrate with ovarian autografts to account for the normal progesterone levels.
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  • 195
    ISSN: 1432-0878
    Keywords: Portal vein ; Rat ; Adrenergic innervation ; Postnatal ; Ontogenesis ; Histofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The postnatal development of the adrenergic innervation pattern in the rat portal vein has been studied with the histochemical fluorescence method of Hillarp and Falck. Stretch preparations and transverse freeze-dried sections of intact portal veins were studied from rats during the first 5 weeks of life and from adult rats. Orientation of undifferentiated smooth muscle cells into two layers was observed at 4 days of age. Dominance of the thick outer longitudinal muscle layer was apparent at two weeks of age. A terminal adrenergic nerve plexus with some varicosities was restricted outside the media at the end of the first week. Ingrowth of penetrating non-terminal adrenergic nerve fibers through the longitudinal muscle layer occurred during the second week of age when the main terminal nerve plexus was developing between the two muscle layers. After 3 weeks of age the adult pattern of a two-dimensional adrenergic plexus between the muscle was established. In the adult rat pharmacological treatment with nialamide and noradrenaline revealed the thin, penetrating non-terminal adrenergic nerve fibers in the longitudinal muscle layer which were poorly visible otherwise. The present observations strongly indicate that the main adrenergic plexus between the two muscle layers emanates directly from the outer axonal plexus. These findings are discussed regarding possible trophic interactions between ingrowing sympathetic adrenergic vasomotor nerves and maturing vascular smooth muscle.
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  • 196
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    Cell & tissue research 172 (1976), S. 195-203 
    ISSN: 1432-0878
    Keywords: Spleen ; Rat ; Marginal sinus ; Marginal zone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The marginal sinus in the spleen of the Wistar rat surrounds the follicle and has more numerous PAS positive fibers on the inner wall than on the outer wall. India ink- and lead oxide-gelatin were injected into the abdominal aorta. It was found that much of the india ink-gelatin accumulated in the marginal sinus, the marginal zone, and part of the red pulp, while most of the lead oxide-gelatin collected in the marginal sinus. Ultrastructurally, the capillaries of the follicle were found to open into the marginal sinus. Regions not perforated by the marginal sinus lie between the follicle and the marginal zone. The wall of the marginal sinus is discontinuous and the discontinuities are wider on the marginal zone side than on the follicle side. The relationship of these findings is discussed.
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  • 197
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    Cell & tissue research 173 (1976), S. 1-15 
    ISSN: 1432-0878
    Keywords: Carotid body ; Rat ; Innervation ; Three-dimensional reconstruction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The innervation of the carotid body of adult rats was studied by means of serial ultrathin sections. A single branching nerve fiber innervates 12 chief cells through several kinds of terminals (vesicle-containing, mitochondrial sack, and calyx-type) in en-passant and bouton forms. Two types of synaptic contacts between nerve terminals and chief cells are found; type 1 in which chief cells are postsynaptic, and type 2 in which chief cells are presynaptic. Since a single nerve fiber (possibly from the glossopharyngeal nerve) forms both types of synapses with type 2 predominating, the nerve fiber is considered basically sensory or centripetal. In addition to their synaptic connections with sensory nerve fibers, chief cells located in the periphery of this organ are in synaptic relation with dendrites of a few ganglion cells adjacent to these cells. Here the chief cells are presynaptic. A few synaptic contacts between two adjacent chief cells are seen, and so are direct contacts between chief cells and preganglionic efferent nerve fibers terminating on ganglion cells.
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  • 198
    ISSN: 1432-0878
    Keywords: Exocytosis, endocytosis ; Freeze-etching ; Electron microscopy ; Membrane particles, rosettes ; Neurohypophysis, neurosecretion ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Images have been obtained from freeze-etch replicas of neurohypophyses which are consistent with the view that orderly arranged aggregates of membrane particles occur in regions where fragments of membrane are being added to and taken away from the plasma membrane during secretion. Aggregates of particles included rosette-like and necklace-like patterns similar to those described by other authors at sites of exocytosis and endocytosis.
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  • 199
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    Cell & tissue research 165 (1976), S. 239-248 
    ISSN: 1432-0878
    Keywords: Superior cervical ganglion ; Rat ; Catecholamines ; Paraganglionic cells ; Monoamine synthesis inhibition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Monoamine storage sites in paraganglionic (PG-)cells of the rat superior cervical ganglion were investigated by electron and fluorescence microscopy following treatment with p-chlorophenylalanine (pCPA), disulfiram or guanethidine respectively. Dense core vesicles in PG-cells are significantly decreased (p〈 0.001) in number following pCPA, and in the majority of these cells following disulfiram and guanethidine. However in a minor portion of PG-cells the latter agents cause an increase in number and in size of dense core vesicles, in parallel with structural alterations. In agreement with these electron microscopic findings fluorescence microscopic and cytophotometric evaluations reveal a general decrease in catecholamine content with few cells showing an increase. The findings provide a morphological basis for the assumption, that monoamine storage sites in PG-cells can be decreased by inhibition of monoamine synthesis, following administration of pCPA, disulfiram and guanethidine. However the two types of responses of PG-cells which occur after disulfiram and guanethidine demonstrate a functional heterogeneity of this cell system in the rat superior cervical ganglion which is discussed.
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  • 200
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    Cell & tissue research 166 (1976), S. 135-143 
    ISSN: 1432-0878
    Keywords: Synaptic ribbons ; Pineal organ ; Rat ; Ultracytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The synaptic complexes of the rat pinealocytes are neither cholinergic nor adrenergic. In the synaptic vesicles, a neurotransmitter carrier substance of lipid nature reacting with OsO4-Zn I2 mixture (similar to that present in both cholinergic and adrenergic vesicles) was not found. In addition, there were no indications of glucose-6-phosphatase or thiamine-pyrophosphatase activity in the synaptic vesicles. Thus, it appears that the synaptic vesicles do not originate from the rough or smooth endoplasmic reticulum. The synaptic ribbons do not contain carbohydrates, are of protein nature and possess some chemical resemblance to microtubules and microtubular bouquets. Appropriate ultracytochemical reactions have not shown detectable quantities of sodium and calcium ions in pinealocyte synaptic complexes.
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