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  • Lipids
  • Wiley-Blackwell  (18)
  • American Chemical Society  (1)
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  • 1
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    Production of two highly abundant 2-methyl-branched fatty acids by blooms of the globally significant marine cyanobacteria Trichodesmium erythraeum (2021)
    American Chemical Society
    Details
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Gosselin, K. M., Nelson, R. K., Spivak, A. C., Sylva, S. P., Van Mooy, B. A. S., Aeppli, C., Sharpless, C. M., O’Neil, G. W., Arrington, E. C., Reddy, C. M., & Valentine, D. L. Production of two highly abundant 2-methyl-branched fatty acids by blooms of the globally significant marine cyanobacteria Trichodesmium erythraeum. ACS Omega, 6(35), (2021): 22803–22810, https://doi.org/10.1021/acsomega.1c03196.
    Description: The bloom-forming cyanobacteria Trichodesmium contribute up to 30% to the total fixed nitrogen in the global oceans and thereby drive substantial productivity. On an expedition in the Gulf of Mexico, we observed and sampled surface slicks, some of which included dense blooms of Trichodesmium erythraeum. These bloom samples contained abundant and atypical free fatty acids, identified here as 2-methyldecanoic acid and 2-methyldodecanoic acid. The high abundance and unusual branching pattern of these compounds suggest that they may play a specific role in this globally important organism.
    Description: This work was funded with grants from the National Science Foundation grants OCE-1333148, OCE-1333162, and OCE-1756254 and the Woods Hole Oceanographic Institution (IR&D). GCxGC analysis made possible by WHOI’s Investment in Science Fund.
    Keywords: Lipids ; Alkyls ; Bacteria ; Genetics ; Chromatography
    Repository Name: Woods Hole Open Access Server
    Type: Article
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    PAPER (OA)
  • 2
    Electronic Resource
    Electronic Resource
    Method of separation and detection of substances on silica gel thin films impregnated with silver nitrate and phosphatotungstic acid (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 575-576 
    Details
    ISSN: 0935-6304
    Keywords: Thin-film chromatography, HPTFC ; Separation on argentized silica gel ; Detection ; Scanning densitometry ; Lipids ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240061012
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  • 3
    Electronic Resource
    Electronic Resource
    Pseudosugars, 34. Synthesis of 5a-carba-β-D-glycosylceramide analogs linked by imino, ether and sulfide bridges (1995)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1995 (1995), S. 267-277 
    Details
    ISSN: 0947-3440
    Keywords: Carbohydrates ; Pseudosugars ; Cyclitols ; Glycoceramide analogs ; Lipids ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Carbocyclic analogs of glycoceramides, (2S,3R,4E)-1-(5a-carba-β-D-glycopyranosyl)-2-(hexadecanoylamino)-4-octa-decen-3-ols E-3-E-6, linked by imino, ether and sulfide bridges, were synthesized by coupling of aziridines, as the sphingosine precursors, with protected 1-amino, 1-hydroxy and 1-mercapto derivatives of 5a-carba sugars and subsequent deprotection and N-acylation. Biological assay of 5a-carbaglycosylceramides showed that both imino-linked E-3 and E-4 having gluco and galacto configurations are mild immunomodulators and possess a mild inhibitory activity against gluco- and galactocerebrosidases. These findings prompted us to prepare the corresponding Z-isomers Z-3 and Z-4, which interestingly show a similar enzyme-inhibitory activity.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.199519950236
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  • 4
    Electronic Resource
    Electronic Resource
    Capillary supercritical fluid chromatography of archaebacterial glycerol tetraether lipids (1986)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 9 (1986), S. 182-185 
    Details
    ISSN: 0935-6304
    Keywords: Supercritical fluid chromatography ; Lipids ; Bacterial glycerol tetraethers ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The analysis of bacterial glycerol tetraethers by capillary supercritical fluid chromatography is described. Complete separation of ethers, differing only by the number of cyclopentane rings present in the isopranyl side chains could be achieved. On a 50% methylphenylpolysiloxane coated capillary, the ethers eluted in the order of increasing cyclopentane content. The analysis revealed the presence of two previously unreported structural isomers of glycerol tetraethers. The method can be used qualitatively and quantitatively as a fast and sensitive screening test for such compounds in living organisms and sedimentary organic matter.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240090311
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  • 5
    Electronic Resource
    Electronic Resource
    Impact of freeze substitution on biological electron microscopy (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 24 (1993), S. 400-422 
    Details
    ISSN: 1059-910X
    Keywords: Cryofixation ; Freeze substitution ; Low temperature embedding ; Lowicryl ; Fine structural preservation ; Extracellular material ; Bacteria ; Algae ; Cyanobacteria ; Fungi ; Transgenic plants ; Wheat ; Barley ; Tobacco ; Conidia ; Hyphae ; Haustoria ; Plant pathogen interface ; Molecular preservation ; Retention diffusible elements ; Microanalysis ; Autoradiography ; Fungicides ; Lipids ; Immunocytochemistry ; Protein antigenicity ; Elicitor ; Extensin ; Foreign proteins ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Considering the increasing necessity for improved preparation techniques in biological electron microscopy as a basis for the identification and localization of cellular substances within the compartments of the cell, this review is focussed on the method of freeze substitution as an important link between the cryofixation (ultrarapid freezing) and resin embedding of biological specimens. The theory and practice of freeze substitution is summarized with particular interest in the physical and thermodynamic as well as in the chemical basis of this technique. A survey of practical aspects of the technical process of freeze substitution concerning the equipment and various protocols successfully applied in biological systems is also given. The main advantage of freeze substitution versus conventional chemical fixation is seen in the maintenance of the hydration shell of molecules and macromolecular structures. This results in an improved fine structural preservation, superior retention of the antigenicity of proteins and decreased loss of unbound, diffusible cellular components. Examples of excellent visualization of the ultrastructure of macro-molecular complexes (nucleic acids, extracellular material, membranes etc.), small organisms (bacteria, algae, cyanobacteria and fungi) and large biological samples such as plant and animal tissue as well as the plant-pathogen (fungus) interface and infection structures are presented. Recent data on the molecular characterization of freeze-substituted biological tissue are exemplified with special emphasis on the subcellular detection of soluble components (elements, lipids, proteins and drugs) and the inter-/intracellular localization of proteins including foreign proteins in transgenic plants. The molecular analysis of freeze-substituted specimens is achieved by the combination of low temperature preparation techniques in biological electron microscopy with various detection methods such as X-ray microanalysis, immunocytochemistry and high resolution autoradiography. © 1993 Wiley-Liss, Inc.
    Additional Material: 27 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070240506
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  • 6
    Electronic Resource
    Electronic Resource
    Comparison by capillary SFC and SFC-MS of soxhlet and supercritical fluid extraction of hamster feces (1991)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 14 (1991), S. 401-406 
    Details
    ISSN: 0935-6304
    Keywords: Supercritical fluid chromatography, SFC ; Supercritical fluid extraction, SFE ; On-line combination ; SFC-MS ; Hamster feces ; Lipids ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Components of hamster feces ranging from low molecular weight fatty acids through the expected range of triglycerides have been eluted in a single SFC run with simultaneous pressure and temperature programming. Temperature programming from 140°C to 240°C was required to provide optimum conditions for separation of the fatty acids and to move the elution region of the sterol esters away from that of the triglycerides. Data from chemical ionization and electron impact mass spectrometry of compounds separated by SFC were used to confirm identities suggested by retention measurements and to provide tentative identities of unknown compounds.SFC with flame ionization detection was used to compare Soxhlet extraction, off-line supercritical fluid extraction (SFE), and on-line SFE of the feces. Although samples obtained by Soxhlet extraction and SFE produced very similar chromatograms, SFE required far less time and consumed much smaller quantities of organic solvent.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240140610
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  • 7
    Electronic Resource
    Electronic Resource
    Separation and identification of unsaturated fatty acid isomers in blood serum and therapeutic oil preparations in the form of their oxazoline derivatives by GC-MS (1994)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 17 (1994), S. 519-521 
    Details
    ISSN: 0935-6304
    Keywords: Capillary gas chromatography ; Mass spectrometry ; Fatty acids ; 2-Substituted 4,4-dimethyloxazoline derivatives ; Lipids ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Fatty acids from blood serum lipids and fish oil preparations are derivatized with 2-amino-2-methyl-1-propanol to form 2-substituted 4,4-dimethyloxazolines. The derivatives are separated on a 25 m × 0.25 mm FFAP column and identified by mass spectrometry using electron impact ionization. The positions of the double bonds of mono- and polyunsaturated fatty acids can be easily and reliably recognized from a characteristic fragmentation feature of the oxazolines. Several unsaturated C16 to C22 fatty acid isomers are identified whose exact structure has not been derived from the commonly used methyl esters.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240170703
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  • 8
    Electronic Resource
    Electronic Resource
    3,6-Epoxyalkansäuren (Tetrahydrofuran-2-essigsäuren) - neue Bestandteile des Humanplasmas (1989)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1989 (1989), S. 449-452 
    Details
    ISSN: 0170-2041
    Keywords: 3,6-Epoxyalkanoic acids ; Fatty acids ; Lipids ; Plasma, human ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: 3,6-Epoxyalkanoic Acids (Tetrahydrofuran-2-acetic Acids) - New Compounds of Human PlasmaHuman plasma contains cis,trans isomers of 3,6-epoxyalkanoic acids 2a-i, a previously unknown class of natural products. These compounds occur in human plasma mainly as free acids.
    Notes: Humanplasma enthält die cis,trans-isomeren 3,6-Epoxyalkansäuren (2,5-substituierte Tetrahydrofuranmonocarbonsäuren) 2a-i. Diese bisher unbekannte Naturstoffklasse liegt im Humanplasma hauptsächlich als freie Säuren vor.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.198919890179
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  • 9
    Electronic Resource
    Electronic Resource
    High-power 13C and 1H nuclear magnetic resonance in dry gluten (1988)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 26 (1988), S. 245-251 
    Details
    ISSN: 0749-1581
    Keywords: Gluten ; Relaxation ; Solid-state NMR ; Lipids ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: High-resolution, solid-state 13C NMR spectra of wheat gluten obtained under a variety of acquisition conditions are reported. These spectra are compared with the results from a 1H relaxation study of dry gluten in which the transverse relaxation times, the spin-lattice relaxation times in the rotating frame and the spin-lattice relaxation times in the laboratory frame at three different Larmor frequencies are characterized. It is concluded that 13C experiments, the proton transverse relaxation measurements and the rotating frame results distinguish between protein and lipid motional environments, whilst the laboratory frame proton spin-lattice relaxation results detect two separate protein environments. The relationship between the laboratory frame spin-lattice relaxation time constants and Larmor frequency is consistent with a model of macromolecular motion in which the polymer chains are held between cross-link points. Observed changes in the various proton relaxation times on removal of lipid or disruption of disulphide cross-links are interpreted in terms of the frequency and anisotropy of molecular motions in the gluten system.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260260313
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  • 10
    Electronic Resource
    Electronic Resource
    F-Säure-haltige Phospholipidmoleküle in Zuckerrohrzellen (1993)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1993 (1993), S. 121-124 
    Details
    ISSN: 0170-2041
    Keywords: Furan fatty acids ; Saccharum spec. ; Cell suspension culture ; Phospholipid molecular species ; Fatty acids ; Lipids ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: F Acid-Containing Phospholipids in Sugar Cane CellsHeterotrophic suspension cultures of sugar cane (Saccharum spec.) cells contain the F acids 1b-f. These acids are bound preferably to phosphatidylethanolamine 3 and phosphatidylcholine 4. Structures of F acid-containing phospholipid molecular species 3b-d, 4b and 4c were established by enzymatic positional hydrolysis and GC/MS analysis of the respective (trimethylsilyl)diacylglycerol derivatives 5.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.199319930122
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