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  • 1
    Publication Date: 2021-05-19
    Description: Study of survey health management and diseases in hatcheries and fish farms can help us to knowledge and application control methods such as: prevention, treatment and increase high levels of production in hatchery and farms, finally. This survey carried out from 2005 to 2008 for 4 years in sturgeon hatcheries and farms of Golestan province. Sturgeon fishes include Huso Huso, Ship sturgeon, Acipenser persicus collected and for virology, bacteriology, fungius and hematology examined. Also, physicochemical parameters measured and recorded in different stages of culture. Results of this study showed that all of samples in virology was negative and did not observe any doubetful causes. In bacteriology CFU was variation from 3/9 ×105 to 6/9×10. The most parasites that detected in this survey was Cocolanus espherolanus , Sceria binopsulus semiarmatus and Amphilina fuliacea that separates from Acipenser Percicus, especially. The results about hematology parameters some important hematological indices of ship sturgeon include: The total RBC for female and mail specimens measured as 5.3±1.5 ×105, 4.8±0.5 ×105 per mm 3 respectively. The amount of haematocrit and hemoglobin for female and mail determined: 34.3±2.8, 35±1.4 percent and 10.3±0.9, 8.9±0.8 gr/dl .The MCV: 216.3± 96.2, 736.5± 102.5, MCH: 720.2±309.5, 186±0.7 and MCHC: 30±0.8, 25.5±3.4 percent respectively.The total WBC were (female, male): 21320±1054, 20580±777 per mm 3 and neutrophil: 16.4±2.5, 17±1.4 percent and lymphocyte: 74.4±2.4, 73.5± 0.7 percent and eosinophil: 6±1.4, 6.4±0.5 percent, monocyte: 2.8±0.8, 3.5±0.7 percent. There was not any significant differences (p〉0.05) between mentioned parameters in male and female (students t-test). Also evaluation of hematological parameters in bluga ( Huso huso) include: total RBC were (male , female) 5±0.3 ×105 , 4.9±0.6 ×105 per mm 3 ,respectively and hematocrit: 33.2±6.7 , 35.4±3.4 percent and hemoglobin: 11.2±1.5 , 12.2±1gr/dl and MCV: 669.9±172.2, 723.9±982.4 and MCH: 226.2±42.5, 249.5±35.4 and MCHC: 34.1±2.4, 34.6±3.6 percent respectively. The totals WBC were (male, female): 24800±707.1, 23042±1375.4 per mm 3 and neutrophil: 18.5±0.7, 21.4±1.1 percent and lymphocyte: 73.5±1.4, 68.4±1.1 percent and eosinophil: 5±2.8, 7±1.2 percent and monocyte: 3.5±3.5, 3.2±0.8 percent. According to statistically study the count of lymphocyte had significant difference between male and female fish and this count in male was higher than female. (p≥0.05).
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physicochemical ; Hematology ; Fish ; Sturgeon ; Huso huso ; Ship ; Acipenser percicus ; Bacteriology ; Parasitology ; Health management ; Diseases ; Survey ; Aquaculture ; Hatcheries ; Samples ; Sceria binopsulus ; Amphilina fuliacea ; Female ; Male
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 395pp.
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  • 2
    Publication Date: 2021-05-19
    Description: This investigation carried out for the first time in Iran inorder to prodcution of monosex female and also sterilization in Rainbow trout. In this study, the eggs of general females were fertilized with the sperm of sex reversed male and so monosex female population was produced in second generation and sterilization carried out with oral administration of 17α methy 1 testosterone and immenrsion and oral administiration methods were used in embryonic stage and from commencing of acitve feeding of larvae, respectiverly. For sex reversal , 13 treatments were considered totally, that the most percentage of male (100%) was observedc in a treatment including of orally administration of 0.5 ppm hormone for 60 days after commencing active feeding (P〈0.001). In the other treamtnet, different percentages of sex ratio including male, female, intersex and sterility were observed. The offspring of genral eggs fertilization with the sperm of masculinized fish were 100% female, chisquare test was shown the treatment of orally administration of 30 ppm hormone for 120 days after commencing active feeding that had been considered for sterilization, was produced 90% sterile fish (P〈0.001) and was changed the sex ratio significancthy. Morphological changes of the gonads and sperm ducts in matured fish and also histological changes in the gonads of fish in the treamtints were considerable.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Morphological ; Histological ; Monosex ; Female ; Male ; Sterilization ; Rainbow trout ; Eggs ; Fertilized ; Sperm ; Population ; Sex ; Fish ; Oncorhynchus mykiss ; Rainbow trout
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 58pp.
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  • 3
    Publication Date: 2021-05-19
    Description: During last 65 years the catch of mullets had increasing trends with some fluctuations in the Iranian coastal water of the Caspian Sea .In this period about 138 thousand tons of mullets have been caught. Mullet’s account for 35% of total catch annually .In recent years species composition of mullets has chanched in the Iranian coastal water of the Caspian Sea and catch composition of golden grey mullet increased from 76% in 1995 to 98% in 2006. In this survey some biological characteristics of golden grey mullet have been studied in Iranian coastal water of the Caspian Sea .Fish samples have been gathered from commercial catch of beach seine cooperatives monthly in Iranian coastal water of the Caspian Sea over 2006 and 2007. In the laboratory fishes have been measured biometrically and biological parameters have been calculated .Also catch statistics of mullets during 2006-2007 have been obtained and discussed. Results showed that the catch of mullets in beach seine cooperatives during 2006 and 2007 was 4181 and 3685 tons respectively that golden grey mullet contribute 99% and 98% of the catch composition of mullets respectively. Length range of golden grey mullet caught by beach seine cooperatives was 19-50.2 cm with mean length of 32.7 ± 6.4 (± SD) and weight range was 67-1475 gr with mean weight of 411 ± 255 gr. The age structure of this species was comprised 2-10 years old fish with mean age of 4.42 years old. In this survey totally the sex ratio of male:female of golden grey mullet was 356: 434 that was significant variation from equal sex ratio. Pick of the spawning in Guilan province was in October and in Mazandaran and Golestan provinces was in November. In October the proportion of spawning females declined from western area (Guilan province) towards eastern parts (Golestan province).The highest proportion of spawning females was in December in Golestan province. The highest GSI index value was observed in September and October and it was decreased in November and December and it was consistent during January till April. The mean absolute fecundity was 700881 ± 429987 eggs with minimum and maximum fecundity of 200112 and 2282862 eggs respectively. The Lm 50% for female golden grey mullet was calculated as 33.6 cm.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biological ; Commercial ; Golden grey mullet ; Liza aurata ; Species ; Survey ; Samples ; Male ; Female ; Sex ratio ; Spawning ; GSI ; Fecundity ; Coastal waters
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 56pp.
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  • 4
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: The biological aspects of Sepia pharaonis was studied during years 2006-07. The studied area restricted to the Bahrekan in Khouzestan province covering the depths of 2 up to 25m. The sampling methods were gillnet and bottom trawl. A total of 310 specimens collected, of which there wasn’t found any cuttlefish in the study area from July to October (5 months). The collected samples were transferred to the laboratory ashore for further biological measurements consist of: Mantle length, Body weight, sex determination. Gonado-Somatic Index, and determination of Spermatophoric Index, Spawning season, Food preference, Maturity stages and chemical analysis for food value determination. The results showed that the overall sex ratio is about M:F= 2:1 with percentage of 67.41% for males and 32.50% for females. Males are significantly bigger than females with average mantle length (ML) of 233.3 and 269.3 mm for female and male, respectively; with body weight of 1102.3 and 1450.6 g. The mantle length body weight relationship was found W=0.001 ML 2.540 (R2= 0.92) Female as: W= 0.0015 ML 4797 (R2= 0.93) male From point of feeding, the food preferences results indicated that fish is considered as main food, crabs as minor food and other marine organisms such as bivalvia and gastropods as random food. The highest vacuity Index (CV) and empty stomachs was determined for March-April and the lowest value was is December. Also, the maximum GSI was estimated for March-April months in which showing coherrances with the lowest food preference. The maximum spermatophoricfilaments were 856 and 45 for male pharaoh cuttlefish with mantle length of 300 and 185 mm, and on the other hand this values for fecundity were estimated 1589 and 53 for female specimens with 254 and 198 mm mantle length. The spawning season occurs in April- March in which accompany with migration of pharaoh cuttlefish towards shallow waters. The fishing season would be in this period in w.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biological ; Chemical ; Sepia pharaonis ; Gillnet ; Sampling ; Specimens ; Weight ; Sex ; Gonado-somatic index ; Spawning ; Maturity ; Female ; Male ; Bivalvia ; Gastropods
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 85pp.
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  • 5
    Publication Date: 2021-05-19
    Description: 0Genetic polymorphism by means of biochemical genetic markers using polyacrylamide gel electrophoresis system in four barbus fish species has been investigated. Species scientific name were Barbus sharpeyi ; Gunther, 1847 , Barbus grypus ; Heckel, 1843, Barbus xanthopterus ; Heckel, 1843 and Barbus esocinus ; Heckel, 1843.Sampling site and location were mainly in the rivers located in Khoozestan province such as Karoon and Karkheh rivers and the Dez dam. Different organs such as blood, muscle, kidney, eye and heart were sampled and analysed for the presence of tf, es, sod and pgm alleles. High polymorphism and presence of different alleles scored, but populations were not in the H-W equilibrium. Considring results, using current and avaiable genetic markers such as microsattelits is recommended for future works.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chemistry ; Genetics ; Genetics Markers ; Distinguish ; Barbus ; Species ; Barbus sharpeyi ; Barbus xanthopterus ; Barbus esocinus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 62pp.
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  • 6
    Publication Date: 2021-05-19
    Description: This study was aimed to investigate the effects of different doses of two hormones and an anti-aromatase, i.e. 17 methyl testosterone (MT), methyl di hydrotestosterone (MDHT) or mestanolone and letozole in masculinization of Nile tilapia (Oreochromis niloticus) under the condition of brackish water in Bafgh station situated in Yazd province in center of Iran. Each experiment in this study was consisted of 5 treaments with 3 replicates each. A number of 1725 larvaes was distributed randomly among 15 replicates at the beginning of each experiment. Each experiment lasted 45 days and the larvaes were reared in aerated flow-through pots and fiberglass tanks filled with brackish water. The averages for temperature, salinity, pH and dissolved oxygen of water were 26.9 ê, 8 g/l, 7.6 and 5.78% respectively during this study. In experiment 1, three different doses of 40, 60 and 100 mg MT/k of feed were fed to different groups of 7 day post fertilization (dpf) larvaes for 45 days from the beginning of the experiment. The results showed that the larvaes in 40 mg group were 100 percent masculinized based on squash test performed at the end of the experiment but masculinization rates of those in 60 and 100 mg groups were 99.7 and 96.2 perecent respectively. Based on Dunkan test, total body length and weight averages measured in biometry 3 (at the end of the experiment) were not significantly different among groups but in biometry 2 (30 days after the beginning of experiment), they were significantly lesser only in 40 mg group (P〈0.05). There was significant differences in survival rate of different groups of larvaes in this experiment based on chi-square test (χ=31.166, P〈0.05) and the values in 40 and 100 mg groups (74.5 and 82.9% respectively) were lesser than those in 60 mg, control 1 and control 2 groups (84.3, 89.0 and 87.0 respectively). In experiment 2, masculinization rates of two different groups of larvaes immersed in 1800 µg MDHT/liter once in 10dpf and twice in 10 and 14dpf were 80.0 and 91.9 percent respectively. There were no significant differences in total body length and weight averages measured in biometry 2 between different groups but significant differences were observed in total body length only in biometry 3 (P〈0.05) where the highest values occurred in experiment 1 and control 1 groups and the lowest one in experiment 2. Significant differences observed in survival rate of different groups of larvaes in this experiment based on chi-square test (χ=15.165, P〈0.05) and the rates in experiment 1, control 2 and 3 groups (89.9, 86.4 and 89.9% respectively) were higher than those in experiment 2 and control 1 groups (82.0 and 82.3 respectively). In experiment 3, three different doses of anti-aromatse letrozole (200, 300 and 400 mg/k feed) were fed to different groups of 7 day post fertilization (dpf) larvaes for 45 days from the beginning of the experiment. The larvaes in 400 group .were all masculinized whereas the masculinization rates in 200 and 300 mg groups were 99.0 and 99.6% respectively. There were significant differences in total body length and weight averages measured in biometry 2 and 3 among groups in this experiment (P〈0.05) where the highest and the lowest values occurred in control 2 and experime2 groups respectively. Based on chi-square, the survival rate of different groups was significantly different (χ=41.119, P〈0.05) and the lowest rate occurred in experiment 2 group. No significant differences observed in gender ratios whithin all control groups in this study based on chi-square test. According to the findings aquired under the condition of brackish water at the present study, it would be potentially recommended to use MT and letrozole for the production of all male polpulations of Nile tilapia fish in order to provide fish farmers with no harmful environmental impacts on water sources in rivers and seas which occured due to the uncontroled breeding of tilapia. However, more research is needed to draw firm conclusions to use hormones and in especial anti-aromase letrozole because of the shortage of sufficient data in current references.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Masculinization ; Nile tilapia ; 17α-methyl testosterone ; Methyl di hydro testosterone ; Mestanolone ; Body weight gain ; Total body lenght ; Brackish water ; Male ; Oreochromis niloticus ; Hormones ; Temperature ; Salinity ; pH ; Dissolved oxygen ; Fertilization ; Survival rate ; Larvae ; Investigation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 61pp.
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  • 7
    Publication Date: 2021-05-19
    Description: Biological characteristics of Liza klunzingeri were studied in two coastal areas, Sajaphi and Bahrekan, of eastern Khuzestan during March to February 2007. Among total 1880 measured fish specimens, 947 specimens were analyzed. The mean value of Gonado-somatic Index (GSI) for the male and female fish were calculated as 0.96± 1.39 and 3.25 ± 3.26 respectively. The GSI value was highest in November and lowest in July. The mean value of condition factor (K) was 1.25± 0.14 in male and 1.21± 0.15 for female. The highest K value were observed in June and the lowest value in February. The lenght at first maturity regardless of sexuality, was found to be 14.5 cm and the time of spawning based on reproduction pattern were determined in Nov- Dec. The length-weight relationship were calculated as Y=0.024L^2.76 (n=226R2=0.72) for males, Y=0.011L^3.00 (n=444R2= 0.78) for females and Y=0.0208L^2.82 (n=670R2 =0.82) for total fishes and also it’s found significant in level lengthweight relationship in (P〈0.05). According to biological characteristics and referring to American fisheries society (AFS) indices and Fuzzy logic expert system, Lize klunzingeri is classified as low vulnerable species.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Lize klunzinger ; Gonado-somatic Index ; GSI ; Condition factor ; Biological characteristics ; Female ; Male ; Specimens ; Fisheries
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 39pp.
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  • 8
    Publication Date: 2021-05-19
    Description: Cheshmehkileh River and adjacent mountainous streams, play a strategic role as a historical axis for anthropogenic civilization, human welfare also habitat and migration pathway of commercial – biologic valuable fishes e.g. Caspian trout, Caspian kuttum, members of Cyprinidae family in south Caspian Sea drainage. Treats such as overfishing of Caspian trout and Red spotted trout stocks in mountainous headwaters, barriers construction and manipulations those are out of river carrying capacity developed by human activities, affected normal function of river as well. Sand mining big factories establishment next to the river, legal and illegal trade of river sediments, direct entry of Tonekabon landfill leakage into the river, development of Rainbow trout farms since 3 decades and huge effluents into the river containing dead fish and types of solids, escapement of cultured Rainbow trouts into the river, … are major minimum factors which needs basic information for integrating inclusively drainage management system. Cheshmehkileh River contains Headwaters of Dohezar (Daryasar & Nusha), Sehezar and Valamroud rivers during 13 monthly sampling phases between September 2009 and October 2010 based on macrozoobenthoses investigations by EPT, EPT/C EPA protocols, measurements of nominated physic-chemical and microbiologic parameters. Probability of Rainbow trouts escapement and invasion, existence, nutrition in Cheshmehkileh environment indeed investigated. Data analysis explained significant differences (P〈0.05) between groups of measured parameters in different sampling stations. Dendogram of clustered analysis based on consolidation of major biologic/ physic-chemical and microbiologic parameters, separated stations No. 1, 3, 2, 4 in one group and remained classified in different groups. Station 8 and 9 similarly separated which expressed general similarities according to Sehezar river environment which were differs in comparison with other stations. Station 11 separated according to its natural quality of water and environment. Similarities between station 10 to Sehezar river stations 8 and 9 expressed general influence of Sehezar River more than Dohezar River in Cheshmehkileh condition especially in station No. 10. High scores of EPT and EPT/C indices in upstream stations 1, 3 and 8 also low score of indices in stations 7, 13 and 6 expressed levels of environment quality between these groups of stations. Maximum average biomass of macroinvertebrates belongs to Trichoptera order in Cheshmehkileh River. Significant decrease of biomass in stations 11, 12 and 13 in comparison with other stations stated environment degradation in mentioned stations relevant to excessive sand mining as well. Pollution resistant groups of invertebrates significantly increased in downstreams against upstream stations. Also disappearing of Plecoptera order in station No. 7, 9, 10 and 13 stated low quality of environment in comparison with upstream stations. Confirmation of effects quality and quantity for point and non-point sources of imported pollutants require specific management considerations in order to present exploitations, pollutants control and emergencies for river monitoring in forthcoming years.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: River ; Pollution ; Aquaculture ; EPTC ; Assessment ; Macroinvertebrates ; Chemistry ; Microbiology
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 138pp.
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  • 9
    Publication Date: 2021-05-19
    Description: The most important habitats of mudskippers are muddy areas in tidal zone of tropical mangrove forests. Mudskippers are related to Oxudercinae subfamily of Gobiid fishes. Three most distributed species of Hormozgan mudskippers were Periophthalmus waltoni, Boleophthalmus dussumieri and Scartelaos tenuis. These fishes can be considered as euryhaline and eurythermal aquatic species, because they can tolerate a wide range of salinity and temperature. A research was done since september 2008 to september 2009 in two important mangrove regions of Hormuzgan (Tyab and Khamir) to determine some ecological characteristics of inhabited mudskipper species. Results showed that nitrate levels are significantly different between tidal lines and seasons (P〈0.05). Maximum nitrite concentrations were recorded 53.2 and 92.5 µg/l in Khamir and Tyab respectively. The annual correlation matrix showed that a positive correlation between phosphate concentration and nitrite and silicate (P〈0.05). Silicate concentration was very high, because of too low density of diatoms and radiolarians. Some species of diatoms, dinoflagellates, cyanobacteria and larvae of crustacea and echinoderms were observed with different density and diversity. Sediment composition of the studied areas were categorized in three classes (clay, sand and clay - sand). Polychaetes formed dominant group of benthic fauna in Tyab and Khamir areas. High density of capitellid worms was possibly related to some environmntal stress caused by activity of fishing and cargo vessels. It was not observed significant difference between fishes length in two areas (P〈0.05); Mean lengths of P. waltoni, B. dussumieri and S. tenuis were calculated 9.85, 14.7 and 11.5 cm respectively. Spawning period of each three species in both areas were obtained from late winter to late spring based on gonadosomatic index values. Male to female sex ratio of P. waltoni, B. dussumieri and S. tenuis were calculated 1:0.45, 1:0.41and 1:0.74 respectively. Absolute fecundity of P. waltoni, B. dussumieri and S. tenuis were estimated 3558 ± 2202, 3952 ± 1030 and 6742 ± 1939 respectively. P. waltoni feeds mainly on fiddler crab, S. tenuis uses crustaceans and gastropods and B. dussumieri has a vegetarian diet.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Mudskippers ; Ecology ; Periophthalmus waltoni ; Boleophthalmus dussumieri ; Scartelaos tenuis ; Female ; Male ; Benthic fauna
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 97pp.
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  • 10
    Publication Date: 2021-05-19
    Description: Aquaculture for human consuming species is being considered as the first substitution of catching aquatic species due to increase of human population and decrease of wild aquatic stocks. In this study, the hybrid sturgeon Bester (female beluga x male sterlet ) was produced for the first time in Iran. Sperm of 1.35 kg male Acipenser ruthenus was used to fertilize the eggs of 125 kg female Huso huso in Shahid Marjani Sturgeon propagation center (Agh Ghala, Golestan province). The fries of bester and control treatment of beluga were transported to International Sturgeon Research Institute (Rasht) after about one month by reaching to 490 mg and 377 mg of weight respectively. All fishes fed by artificial concentrated food (48-50% protein and 15-17% fat) after a period of feeding with Artemia and Daphnia. Sorting was carried out according to increase of fish weight for both fishes. Results showed that the imported sterlet spawners were not at the high maturation stages and especially the males had not suitable sperm quality. It showed that up to 2 months of age , these was no significant difference between bester and beluga weight but from this age up to 2 months of age the weight of beluga was greater. Meanwhile from 2 months old up to the end of the study (21 months) the weight of bester sample was significantly greater than beluga. The comparison of FCR for the whole rearing period showed no difference between bester and beluga (2.4 and 2.3 respectively). In general, the increase and decrease pattern of GR and SGR were coincided to each other, but showed monthly differences. Growth rate (GR) and specific growth rate (SGR) of bester were greater than beluga from 4th and 3rd month of rearing period respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Aquaculture ; Beluga ; Sterlet ; Bester ; Growth Rate ; Aquatic ; Species ; Population ; Female ; Male ; Acipenser ruthenus ; Huso huso ; Sturgeon ; Artemia
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 55pp.
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