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  • Biochemistry and Biotechnology
  • 1970-1974  (501)
  • 1965-1969  (352)
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Keywords
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  • 1
    Electronic Resource
    Electronic Resource
    Starter populations as determined under steady state conditionsContribution No. 1561 from the WHOI. Supported in part by Grant No. 861 of the National Science Foundation. (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 279-283 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Continuous cultures of some heterotrophic Spirilli (Sp. serpens, Sp. curvatum, Sp. sp.) exhibit threshold concentrations of lactate, the growth limiting carbon and energy source, around 15 mg./l. This value corresponds to a threshold population density of 0.92 mg. dry weight/l. at a dilution rate of D = 0.185 hr.-1 (= 0.5 maximum growth rate). The minimum population density, which is regarded as equivalent to the starter population in batch culture, was dependent on the growth rate and the redox potential. The existence of minimum populations and the pronounced effect of population density on growth at low nutrient levels seem to be widespread phenomena closely related to growth under suboptimal conditions.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070207
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  • 2
    Electronic Resource
    Electronic Resource
    Resistance of microorganisms to hydrostatic pressure (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 139-159 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A small fraction, about 0.05%, of the microorganisms in milk are resistant to pressure to at least 150,000 p.s.i. The pressure-resistant microorganisms were shown to be mainly bacterial spores. Some of the pressure-resistant organisms were identified. The lethal effect of pressure is more pronounced in the solid phase than in the liquid phase of water. B. subtilis was found to be capable of surviving solid-phase transitions from Ice II, III, and V to Ice I. The lethal effect of high pressure in the liquid phase of water was found not to be counteracted by an increase in temperature. The pressure resistance of B. subtilis was found to be maximum around neutral or slightly basic pH. The damaging effect of low and high pH are counteracted by sodium chloride and glucose. It is suggested that the primary mechanism of protein denaturation by pressures above 30,000 p.s.i. is ionization and formation of ionic bonds between charged groups on proteins, altering their solubility.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070124
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  • 3
    Electronic Resource
    Electronic Resource
    Electrochemical observations in microbiological processes. Growth of Thiobacillus Thiooxidans. II (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 491-506 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The electrochemical activity of the individual chemicals in the nutrient medium for growth of Thiobacillus thiooxidans was studied along with the effect of the gases in equilibrium with their solutions. Several chemicals were active individually; however, the magnitudes, as measured by changes in half-cell potential, were less than that observed when T. thiooxidans was present in their composite mixture. Sterilized Skerman's mineral salts and the American Type Culture Collection (without ferrous sulfate) media were not sensitive electrochemically to changes from pure oxygen to nitrogen atmospheres. When T. thiooxidans was present in these media, the biological half-cell emf became sensitive to changes in the oxygen content of the atmosphere in equilibrium with the organism and nutrient. The ability of T. thiooxidans to make an electrochemical contribution, as registered by a platinum electrode, is substantiated further by these investigations.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070405
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  • 4
    Electronic Resource
    Electronic Resource
    Production of Bacillus cereus exopenicillinase on a pilot-plant scale (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 517-528 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A substrain of Bacillus cereus 569/H produced above 10,000 units of penicillinase/ ml. when grown in a pilot-plant fermentor using a medium containing Casamino acids techn. (Difco) or N-Z-Amine type B (Sheffield), and salts. Simplified purification and concentration procedures give an overall yield of 50-65% enzyme. The freeze-dried enzyme preparation had a good storage stability in vacuumsealed ampules kept at 4, 30, and 37°C. In vials containing air in the head space, partial inactivation occurred in two months at 30 and 37°C. The freezedried preparation showed satisfactory performance in the production of yoghurt fermented milk.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070407
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  • 5
    Electronic Resource
    Electronic Resource
    Process equipment (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 8-10 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070104
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  • 6
    Electronic Resource
    Electronic Resource
    Kinetics and continuous culture (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 13-16 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070106
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  • 7
    Electronic Resource
    Electronic Resource
    Amino acids (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 21-29 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070108
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  • 8
    Electronic Resource
    Electronic Resource
    An automatic aeration control system for biosynthetic processes (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 91-100 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An automatic aeration controller was constructed to assure the optimal amount of oxygen for aerobic biosynthetic processes. A platinum electrode and a KCl bridge for the calomel electrode were inserted hermetically into the fermentor and were sterilized with the culture medium. The electrode potential developed at the surface of the Pt electrode continuously indicated the actual oxygen concentration of the medium. This served as the controlled variable of the system. The concentration of dissolved oxygen resulted from the actual demand and supply of the microorganism. The electrode potential, which was the measured variable of the process, operated a closed-loop automatic aeration system. The controller activated an air inlet valve in order to balance the controlled variable. The automatic controller operated in fermentors of industrial scale for long periods in a satisfactory manner.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070120
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  • 9
    Electronic Resource
    Electronic Resource
    Improved model of a steam-sterilizable electrode for dissolved oxygen determination in culture media (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 8 (1966), S. 621-624 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260080412
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  • 10
    Electronic Resource
    Electronic Resource
    Masthead (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965) 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260070201
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