ALBERT

Description: During software development activities, it is important for Information and Communication Technology (ICT) practitioners to know and understand practices and guidelines regarding information privacy, as software requirements must comply with data privacy laws and members of development teams should know current legislation related to the protection of personal data. In order to gain a better understanding on how industry ICT practitioners perceive the practical relevance of software privacy and privacy requirements and how these professionals are implementing data privacy concepts, we conducted a survey with ICT practitioners from software development organizations to get an overview of how these professionals are implementing data privacy concepts during software design. We performed a systematic literature review to identify related works with software privacy and privacy requirements and what methodologies and techniques are used to specify them. In addition, we conducted a survey with ICT practitioners from different organizations. Findings revealed that ICT practitioners lack a comprehensive knowledge of software privacy and privacy requirements and the Brazilian General Data Protection Law (Lei Geral de Proteção de Dados Pessoais, LGPD, in Portuguese), nor they are able to work with the laws and guidelines governing data privacy. Organizations are demanded to define an approach to contextualize ICT practitioners with the importance of knowledge of software privacy and privacy requirements, as well as to address them during software development, since LGPD must change the way teams work, as a number of features and controls regarding consent, documentation, and privacy accountability will be required.

Description: In this paper, a direct power predictive controller (DPPC) is derived for a current source inverter (CSI) based single stage photovoltaic (PV) system. The equations of the dynamics, including AC and DC filters, are formulated directly for the PV power and for the active and reactive power injected in the grid. Then, the prediction equations are synthesized straight for the power, and, at each time instant, the optimal switching vector that guarantees simultaneously the control of the power generated by the PV arrays, and the control of the reactive power in the connection to the grid is chosen. This approach aims to guarantee fast and accurate tracking of the power. The proposed system is then validated through simulation and experimental results, showing that the PV system is able to follow the power references, guaranteeing a fast response to a step in the power, and decoupled active and reactive power control, with minimum total harmonic distortion (

Description: The CE 1755 Lisbon tsunami was the largest historical tsunami to affect the Atlantic coasts of Europe and North Africa. This study presents the results obtained from the application of different sedimentological techniques (e.g., grain size, morphoscopy, microtextural analysis, geochemistry, radiocarbon dating) on sediments retrieved from the Alcabrichel River alluvial plain (of about 500 m far away from its mouth and approximatively 50 km northwest of Lisbon, Portugal). The results allowed the identification of a sandy layer that was associated with the CE 1755 tsunami. Furthermore, a new microtextural semi-quantitative classification was applied to enhance the identification of extreme marine inundation deposits. Based on sedimentological data, three different tsunami inundation phases were identified, including two inundations and a likely backwash. This innovative work offers physical evidence of the spatial presence of the CE 1755 tsunami event on the western coast of Europe. It also enables a reconstruction of tsunami inundation dynamics, with two flooding waves and an interspersed backwash.

Description: Atherosclerosis is one of the most serious and common forms of cardiovascular disease and a major cause of death and disability worldwide. It is a multifactorial and complex disease that promoted several hemodynamic studies. Although in vivo studies more accurately represent the physiological conditions, in vitro experiments more reliably control several physiological variables and most adequately validate numerical flow studies. Here, a hemodynamic study in idealized stenotic and healthy coronary arteries is presented by applying both numerical and in vitro approaches through computational fluid dynamics simulations and a high-speed video microscopy technique, respectively. By means of stereolithography 3D printing technology, biomodels with three different resolutions were used to perform experimental flow studies. The results showed that the biomodel printed with a resolution of 50 μm was able to most accurately visualize flow due to its lowest roughness values (Ra = 1.8 μm). The flow experimental results showed a qualitatively good agreement with the blood flow numerical data, providing a clear observation of recirculation regions when the diameter reduction reached 60%.

Description: Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (Seriola fasciata) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (Bodianus scrofa) caught in the Selvagens Islands (Portugal).

Description: Diarrhetic shellfish toxins (DSTs) are among the most prevalent marine toxins in Europe’s and in other temperate coastal regions. These toxins are produced by several dinoflagellate species; however, the contamination of the marine trophic chain is often attributed to species of the genus Dinophysis. This group of toxins, constituted by okadaic acid (OA) and analogous molecules (dinophysistoxins, DTXs), are highly harmful to humans, causing severe poisoning symptoms caused by the ingestion of contaminated seafood. Knowledge on the mode of action and toxicology of OA and the chemical characterization and accumulation of DSTs in seafood species (bivalves, gastropods and crustaceans) has significantly contributed to understand the impacts of these toxins in humans. Considerable information is however missing, particularly at the molecular and metabolic levels involving toxin uptake, distribution, compartmentalization and biotransformation and the interaction of DSTs with aquatic organisms. Recent contributions to the knowledge of DSTs arise from transcriptomics and proteomics research. Indeed, OMICs constitute a research field dedicated to the systematic analysis on the organisms’ metabolisms. The methodologies used in OMICs are also highly effective to identify critical metabolic pathways affecting the physiology of the organisms. In this review, we analyze the main contributions provided so far by OMICs to DSTs research and discuss the prospects of OMICs with regard to the DSTs toxicology and the significance of these toxins to public health, food safety and aquaculture.

Description: Porphyrins and derivatives form one of the most abundant classes of biochromes. They result from the breakdown of heme and have crucial physiological functions. Bilins are well-known representatives of this group that, besides significant antioxidant and anti-mutagenic properties, are also photosensitizers for photodynamic therapies. Recently, we demonstrated that the Polychaeta Eulalia viridis, common in the Portuguese rocky intertidal, holds a high variety of novel greenish and yellowish porphyrinoid pigments, stored as granules in the chromocytes of several organs. On the follow-up of this study, we chemically characterized pigment extracts from the worm’s skin and proboscis using HPLC and evaluated their light and dark toxicity in vivo and ex vivo using Daphnia and mussel gill tissue as models, respectively. The findings showed that the skin and proboscis have distinct patterns of hydrophilic or even amphiphilic porphyrinoids, with some substances in common. The combination of the two bioassays demonstrated that the extracts from the skin exert higher dark toxicity, whereas those from the proboscis rapidly exert light toxicity, then becoming exhausted. One particular yellow pigment that is highly abundant in the proboscis shows highly promising properties as a natural photosensitizer, revealing that porphyrinoids from marine invertebrates are important sources of these high-prized bioproducts.

Description: Fatigue is one of the main causes for in service failure of mechanical components and structures. With the development of new materials, such as high strength aluminium or titanium alloys with different microstructures from steels, materials no longer have a fatigue limit in the classical sense, where it was accepted that they would have ‘infinite life’ from 10 million (107) cycles. The emergence of new materials used in critical mechanical parts, including parts obtained from metal additive manufacturing (AM), the need for weight reduction and the ambition to travel greater distances in shorter periods of time, have brought many challenges to design engineers, since they demand predictability of material properties and that they are readily available. Most fatigue testing today still uses uniaxial loads. However, it is generally recognised that multiaxial stresses occur in many full-scale structures, being rare the occurrence of pure uniaxial stress states. By combining both Ultrasonic Fatigue Testing with multiaxial testing through Single-Input-Multiple-Output Modal Analysis, the high costs of both equipment and time to conduct experiments have seen a massive improvement. It is presently possible to test materials under multiaxial loading conditions and for a very high number of cycles in a fraction of the time compared to non-ultrasonic fatigue testing methods (days compared to months or years). This work presents the current status of ultrasonic fatigue testing machines working at a frequency of 20 kHz to date, with emphasis on multiaxial fatigue and very high cycle fatigue. Special attention will be put into the performance of multiaxial fatigue tests of classical cylindrical specimens under tension/torsion and flat cruciform specimens under in-plane bi-axial testing using low cost piezoelectric transducers. Together with the description of the testing machines and associated instrumentation, some experimental results of fatigue tests are presented in order to demonstrate how ultrasonic fatigue testing can be used to determine the behaviour of a steel alloy from a railway wheel at very high cycle fatigue regime when subjected to multiaxial tension/torsion loadings.

Description: Due to its properties, paper represents an alternative to perform point-of-care tests for colorimetric determination of glucose levels, providing simple, rapid, and inexpensive means of diagnosis. In this work, we report the development of a novel, rapid, disposable, inexpensive, enzyme-free, and colorimetric paper-based assay for glucose level determination. This sensing strategy is based on the synthesis of gold nanoparticles (AuNPs) by reduction of a gold salt precursor, in which glucose acts simultaneously as reducing and capping agent. This leads to a direct measurement of glucose without any enzymes or depending on the detection of intermediate products as in conventional enzymatic colorimetric methods. Firstly, we modelled the synthesis reaction of AuNPs to determine the optical, morphological, and kinetic properties and their manipulation for glucose sensing, by determining the influence of each of the reaction precursors towards the produced AuNPs, providing a guide for the manipulation of nucleation and growth. The adaptation of this synthesis into the developed paper platform was tested and calibrated using different standard solutions with physiological concentrations of glucose. The response of the colorimetric signals obtained with this paper-based platform showed a linear behavior until 20 mM, required for glycemic control in diabetes, using the Red × Value/Grey feature combination as a calibration metric, to describe the variations in color intensity and hue in the spot test zone. The colorimetric sensor revealed a detection limit of 0.65 mM, depending on calibration metric and sensitivity of 0.013 AU/mM for a linear sensitivity range from 1.25 to 20 mM, with high specificity for the determination of glucose in complex standards with other common reducing interferents and human serum.

Description: Energy harvesting systems for low-power devices are increasingly being a requirement within the context of the Internet of Things and, in particular, for self-powered sensors in remote or inaccessible locations. Triboelectric nanogenerators are a suitable approach for harvesting environmental mechanical energy otherwise wasted in nature. This work reports on the evaluation of the output power of different polymer and polymer composites, by using the triboelectric contact-separation systems (10 N of force followed by 5 cm of separation per cycle). Different materials were used as positive (Mica, polyamide (PA66) and styrene/ethylene-butadiene/styrene (SEBS)) and negative (polyvinylidene fluoride (PVDF), polyurethane (PU), polypropylene (PP) and Kapton) charge materials. The obtained output power ranges from 0.2 to 5.9 mW, depending on the pair of materials, for an active area of 46.4 cm2. The highest response was obtained for Mica with PVDF composites with 30 wt.% of barium titanate (BT) and PA66 with PU pairs. A simple application has been developed based on vertical contact-separation mode, able to power up light emission diodes (LEDs) with around 30 cycles to charge a capacitor. Further, the capacitor can be charged in one triboelectric cycle if an area of 0.14 m2 is used.