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  • 1
    Electronic Resource
    Electronic Resource
    Purification and Properties of a Nucleotide Pyrophosphatase from Lentil Seedlings (2000)
    Springer
    The protein journal 19 (2000), S. 209-214 
    Details
    ISSN: 1573-4943
    Keywords: Lens esculenta ; Leguminosae ; nucleotide pyrophosphatase ; nucleotides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A nucleotide pyrophosphatase (EC 3.6.1.9) was purified to homogeneity from lentil seedlings. The enzyme is a single polypeptide chain of 75 ± 2 kDa that exhibits hydrolytic activities toward pyrophosphate linkages of several substrates. Reduced and oxidized forms of NAD(P) were shown to be hydrolyzed to nicotinamide mononucleotide and AMP. Other dinucleotides such as FAD and dinucleoside oligophosphates were hydrolyzed as well, but with lower efficiency. Pyrophosphatase activity was increased in the presence of divalent cations such as Ca2+, Mg2+, and Mn2+, whereas Cu2+, Zn2+, and Ni2+ ions inhibited this activity. The active site in the enzyme was not defined, but histidine residue(s) seemed to be crucial for the enzymatic activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007007803996
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  • 2
    Electronic Resource
    Electronic Resource
    Catalase Activity of Nodules in the Ureide- and Amide-transporting Legume Plants (2000)
    Springer
    Russian journal of plant physiology 47 (2000), S. 721-727 
    Details
    ISSN: 1608-3407
    Keywords: Leguminosae ; symbiotic nitrogen fixation ; effective and ineffective symbiosis ; root nodules ; catalase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relations of catalase activity to the efficiency of symbiotic dinitrogen fixation and leghemoglobin (Lb) content were investigated in roots and nodules of several legume plant species together with the catalase distribution between the inner bacteroidal and the outer cortical nodule tissues. The catalase activity in the nodules exceeded that of the roots of the amide- and ureide-synthesizing plant species by one and two orders of magnitude. During the growth period, catalase activity and Lb content changed in parallel and reached their highest levels early in the stage of flowering or fruit formation, depending on plant species. In the case of effective symbiosis, catalase activity in the nodules was 2.5–5 times higher than in the case of ineffective symbiosis. Catalase activity in the bacteroidal zone of the nodules was several times higher than that of the cortical tissue, and two nodule tissues differed in catalase activity more notably in the plant species exporting ureides. The authors suggest that high catalase activity in the nodules, especially in their bacteroidal zone, is essential for the efficient functioning of the symbiotic system of dinitrogen fixation in both ureide- and amide-transporting plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026694709210
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  • 3
    Electronic Resource
    Electronic Resource
    Software dependent phenetic relationships of non-nodulating African species ofAcacia (2000)
    Springer
    Plant systematics and evolution 220 (2000), S. 139-146 
    Details
    ISSN: 1615-6110
    Keywords: Acacia ; classification ; Leguminosae ; morphology ; phenetics ; RAPD ; software ; taxonomy ; UPGMA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The phenetic analysis of non-nodulatingAcacia species by Harrier et al. (1997) was repeated to illustrate how different computer programs may generate alternative UPGMA trees for the very same data, even in the absence of data input order effects (ties). For example, all Harrier et al.'s UPGMA dendrograms produced by software from the Scottish Agricultural Statistics Service differed from those obtained by the packages NTSYS and MVSP87. Particularly, the positions ofA. albida, A. rovumae, andA. pentagona, as well as the relationships betweenDiacanthae andTriacanthae were affected by this phenomenon. Hence, whenever clustering techniques are used, care should be taken to consider possible software-dependent caveats and artefacts. Nevertheless, all programs provided clusterings that largely coincided with the subgeneric and sectional groupings proposed by Vassal (1972) although the positions of some species varied depending on whether morphological or molecular data were considered (e.g.A. albida andA. rovumae).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00985042
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  • 4
    Electronic Resource
    Electronic Resource
    Secretory cavities and pellucid dots in leaflets ofLonchocarpus (Leguminosae, Papilionoideae, Millettieae) (2000)
    Springer
    Plant systematics and evolution 221 (2000), S. 61-68 
    Details
    ISSN: 1615-6110
    Keywords: Leguminosae ; Papilionoideae ; Lonchocarpus ; leaflets ; secretory cavities ; false secretory cavities ; pellucid dots ; glands ; anatomy ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The occurrence of leaflet secretory cavities in 23 Brazilian species ofLonchocarpus was surveyed anatomically and compared with data from external morphology (presence of pellucid dots) to evaluate their taxonomic significance. This study revealed three cases: presence of secretory cavities associated with pellucid dots, pellucid dots corresponding to false secretory cavities, and absence of pellucid dots and secretory cavities. These results indicate that in this genus the “glandular pellucid dots” cited in morphological descriptions do not always correspond to secretory cavities, and that their nature must always be confirmed by histological sections. Secretory cavities have systematic significance at subgeneric taxonomic level for the genusLonchocarpus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01086380
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  • 5
    Electronic Resource
    Electronic Resource
    Single-flowered vetch (Vicia articulata Hornem.): A relic crop in Italy (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 461-465 
    Details
    ISSN: 1573-5109
    Keywords: C-banding ; crop genetic resources ; Leguminosae ; Sardinia ; seed quality ; Vicia articulata Hornem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Single-flowered vetch (Vicia articulata Hornem.), a crop native of the Mediterranean area, is widespread mainly in south Europe, in west Asia and Australia for forage, green manure and human food. In Italy this crop was still cultivated in the 1950s but, later, no records concerning its cultivation were available. This strongly suggested to place V. articulata among the Italian crop species that disappeared in the 20th century. In the course of a collecting expedition in Sardinia, a relic population, mistaken for lentil, was found. This population has been characterised from a botanical, nutritional and cytological point of view. V. articulata appears to be lower in nutritional quality than lentil. However, within the framework of a sustainable agriculture, V. articulata could find interesting opportunities. In fact, its role in soil conservation and rescue of marginal areas could be interesting, as well as its potentialities as a source of useful genes in breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008711022396
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  • 6
    Electronic Resource
    Electronic Resource
    In vitro organogenesis and plant formation in Acacia sinuata (2000)
    Springer
    Plant cell, tissue and organ culture 61 (2000), S. 23-28 
    Details
    ISSN: 1573-5044
    Keywords: Acacia sinuata ; Leguminosae ; regeneration ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vitro morphogenesis via organogenesis was achieved from callus cultures derived from hypocotyl explants of Acacia sinuata on MS (Murashige and Skoog, 1962) medium. Calli were induced from hypocotyl explants excised from 7-day-old seedlings on MS medium containing 3% sucrose, 0.8% agar, 6.78 μM 2,4-dichlorophenoxyacetic acid and 2.22 μM 6-benzylaminopurine. Regeneration of adventitious buds from callus was achieved when they were cultured on MS medium supplemented with 10% coconut water, 13.2 μM 6-benzylaminopurine and 3.42 μM indoleacetic acid. Addition of gibberellic acid (1.73 μM) favored shoot elongation. Regenerated shoots produced prominent roots when transferred to half strength MS medium supplemented with 7.36 μM indolebutyric acid. Rooted plantlets, thus developed were hardened and successfully established in the soil. This protocol yielded an average of 20 plants per hypocotyl explant over a period of 4 months.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006442818277
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