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  • Biochemistry and Biotechnology
  • 1960-1964  (58)
  • 1964  (58)
  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 6 (1964), S. 309-319 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The authors describe continuous cultivations of Escherichia coli possessing high penicillin-acylase activity in corn-steep liquor, peptone, and ammonium phenylacetate containing nutrient medium. If the cultivation is performed in the absence of ammonium phenylacetate the enzymic activities of cells from the batch as well as the continuous cultures are very low. The enzymic activity of cells in the batch process was considerably increased by the addition of 0.015% ammonium phenylacetate to the nutrient medium. Further increase of ammonium phenylacetate concentration did not result in any further increase of acylase activity. Continuous cultivation of the bacteria at the above ammonium phenyl-acetate concentration was unsuccessful, as the enzymic activity of the bacteria constantly decreased during the process. On increasing the concentration of ammonium phenylacetate in the medium to 0.15% the authors succeeded in maintaining the enzymic activity of the bacteria at the same level as in the batch process performed at 0.015% concentration, throughout the whole continuous cultivation. At the dilution rate D = 0.5 hr.-1 the concentration of cells in the culture effluent from the fermentor at the steady state was equal to cell concentration at the end of batch cultivation. In relation to the cultivation time the output of cells in continuous cultivation is almost seven times higher as compared with the batch process.
    Additional Material: 3 Ill.
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  • 2
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Steam-sterilizable membrane probes for monitoring the dissolved oxygen level in fermentors, or the oxygen content of gas streams, are described. The probes have a silver cathode, a lead anode, and an acetate buffer as an electrolyte. The membrane is Teflon. The current output of the probes in the absence of oxygen is negligible.
    Additional Material: 5 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 6 (1964), S. 191-221 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Distillers' yeasts, strains of Saccharomyces cerevisiae, although capable of sexual reproduction, in distillery practice reproduce asexually, by budding. A cell may bring forth another one in 50 min. With every ounce of whiskey produced, 30 billion new cells come to existence. Within a few days, in 4-8 propagation stages, a test tube full of culture will populate 100,000 gallons beer with 150 million cells per ml. Rate of reproduction, the number of new cells per each original cell varies from 5 to 50 in the various propagation stages. The number of new cells produced in a given nutrient is independent from the number of initial cells; and the utilization of the nutrient increases with the dilution of the substrate. Although distillers' yeasts may reproduce at such extremes as 1-46°C, 2½-10½ pH, presence of 0-15% alcohol by volume, and 0.1-25% sugar content, in distillery practice the factors are so selected to maintain conditions close to the optimum. When placed in the nutrient the initial cells will measure the chemical and physical characteristics of the new living space and the cell population, and will prepare a design of reproduction best suited to the conditions. The design includes a symmetry in the grouping of the cells and a rhythmic timing in starting new buds. Each healthy cells is biologically equal to the others and is capable of performing all functions characteristic of the strain. In spite of the sensitive coordination system between the individual cells that regulates their activity, marked differences exist among the cells to the degree of cell individuality. Distillers yeasts are superbly equipped to live and reproduce.
    Additional Material: 23 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 6 (1964), S. 469-471 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: It was found that moderate pressures (16.0 to 43.1 atmospheres) perceptibly decreased the rate of α-amylase catalyzed starch hydrolysis. The decreased rate was apparently due to pressure induced deactivation of the enzyme.
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 6 (1964), S. 491-496 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 6 (1964), S. 223-234 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method of performing electrophoretic separations in a thin horizontal layer of continuously flowing buffer has been developed. The theoretical considerations that make possible the use of a thin layer for stabilization against convection effects, without the assistance of a packing material, are discussed. The apparatus is described together with an example of its performance, and a comparison with that of other workers.
    Additional Material: 5 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 6 (1964), S. 235-240 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The operation and construction of a liquid-liquid chromatography apparatus containing differentially permeable partions is described. The method is of value for the separation of heat sensitive and nonvolatile solutes.
    Additional Material: 2 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 6 (1964), S. 367-379 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Continuous flow-type reactors have been used to study the kinetics of biological systems for quite some time. For continuous media sterilization, tubular flow reactors are particularly useful being simple in character and easy to control. However, one aspect quite often neglected in sterilization calculations is the residence time distribution of the reactor system. Serious errors in estimating the degree of bacterial destruction can be encountered if the residence time distribution is neglected; especially when a high degree of destruction is desired. This paper reports a study made to characterize and use the residence time distribution of a tubular reactor in the interpretation of high-temperature, short exposure time data for inactivation of Bacillus stearothermophilus spores. Mathematical models accounting for the residence time distribution of the tubular reactor have been proposed and employed to obtain high-temperature death-rate data.
    Additional Material: 8 Ill.
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  • 9
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A cultivation apparatus made up from six small (80-800 ml.) glass units with independent pH-, aeration-, and foam control is described. Exchangeable attachments made it possible to run the unit both batchwise and continuous and to connect up the units in various fashions.
    Additional Material: 14 Ill.
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  • 10
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Two sources of oxygen for man in closed-cycle space system environment have been considered in previous studies: (1) photosynthesis using algae, and (2) electrolysis of water. The latter system appears to be the most promising from the standpoints of energy and weight requirements and ability to operate in a zero gravity field. The surplus hydrogen produced by the electrolysis of water may be utilized together with waste carbon dioxide, part of the oxygen, and waste urea by bacteria of the genus Hydrogenomonas to produce cellular protein which might be used as a source of food. A continuous culture system for the propagation of hydrogen-fixing bacteria consists of a baffled borosilicate glass culture vessel provided with an impeller, a reservoir vessel for the culture medium, and an overflow vessel for collecting the bacterial cells removed from the culture vessel. Complete feedback control of all parameters affecting growth can be provided by hydrogen, oxygen, and carbon dioxide sensors, and a pH electrode in the culture medium. In addition, total pressure is monitored. Cell density is controlled in the optimum range by means of a photoelectric cell which dictates the amount of fresh medium to be added and the amount of cells to be removed. Operating data indicate that some of the key parameters are the ratio of hydrogen, oxygen, and carbon dioxide in solution. The harvested bacterial substance is high in protein, which contains all the essential amino acids.
    Additional Material: 2 Ill.
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