Publication Date:
2024-05-27
Description:
Ocean acidification (OA) appears to have diverse impacts on calcareous coccolithophores, but the cellular processes underlying these responses are not well understood. Here we use stable boron and carbon isotopes, B/Ca ratios, as well as inorganic and organic carbon production rates to investigate the carbon utilization and the internal pH regulation at the site of calcification in Emiliania huxleyi, Pleurochrysis carterae and Calcidiscus leptoporus cultured over a wide pCO2 range (180 to 1000 μatm). Despite large variability, the geochemistry data indicate species-specific modes of pH control and differences in the utilization of inorganic carbon. Boron isotope data suggest that all three species generally upregulate the pH of the calcification fluid (pHCF) compared to surrounding seawater, which coincides with relatively constant growth rates and cellular ratios of inorganic to organic carbon. Furthermore, species exhibit different strategies in regulating their pHCF, i.e., two species maintain homeostasis (pHCF = ∼ 8.7), while one species shows a constant offset to the surrounding seawater (ΔpH = ∼0.6 units) over the entire tested pCO2 range. In addition to these different strategies, carbon isotope data suggests that high plasticity in the utilization of dissolved inorganic carbon might be an explanation for species-specific differences in coccolithophore responses to OA.
Keywords:
Acid-base regulation; Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Biomass/Abundance/Elemental composition; Boron/Calcium ratio; Boron/Calcium ratio, standard deviation; Bottles or small containers/Aquaria (〈20 L); Calcidiscus leptoporus; Calcification/Dissolution; Calcifying fluid, pH; Calcifying fluid, pH, standard deviation; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, inorganic, particulate, per cell; Carbon, inorganic, particulate, production per cell; Carbon, organic, particulate, per cell; Carbon, organic, particulate, production per cell; Carbon, organic, particulate, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chromista; Difference δ13C, particulate inorganic carbon and dissolved inorganic carbon; Difference δ13C, particulate inorganic carbon and dissolved inorganic carbon, standard deviation; Difference δ13C, particulate organic carbon and dissolved inorganic carbon; Difference δ13C, particulate organic carbon and dissolved inorganic carbon, standard deviation; Emiliania huxleyi; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Haptophyta; Identification; Iron/Calcium ratio; Iron/Calcium ratio, standard deviation; Laboratory experiment; Laboratory strains; Magnesium/Calcium ratio; Magnesium/Calcium ratio, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Other studied parameter or process; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate inorganic carbon/particulate organic carbon ratio; Particulate inorganic carbon/particulate organic carbon ratio, standard deviation; Particulate inorganic carbon per cell, standard deviation; Pelagos; pH; pH, difference; pH, difference, standard deviation; pH, standard deviation; Phytoplankton; Pleurochrysis carterae; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Registration number of species; Salinity; Single species; Species; Temperature, water; Temperature, water, standard deviation; Type; Uniform resource locator/link to reference; δ11B; δ11B, standard deviation; δ13C, dissolved inorganic carbon; δ13C, dissolved inorganic carbon, standard deviation; δ13C, particulate inorganic carbon; δ13C, particulate inorganic carbon, standard deviation; δ13C, particulate organic carbon; δ13C, particulate organic carbon, standard deviation
Type:
Dataset
Format:
text/tab-separated-values, 1945 data points
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