ALBERT

Description: In the present work, the performance of carbon nanotubes (c-CNTs) functionalized polydimethylsiloxane (PDMS) based coatings as extractive phases for in-tube solid phase microextraction (IT-SPME) coupled to Capillary LC (CapLC) has been evaluated. Carboxylic-single walled carbon nanotubes (c-SWNTs) and carboxylic-multi walled carbon nanotubes (c-MWNTs) have been immobilized on the activated surface of PDMS capillary columns. The effect of different percentages of diphenyl groups in the PDMS extractive phase has also been evaluated. The extraction capability of the capillary columns has been tested for different organic pollutants, nitrogen heterocyclic compounds and polycyclic aromatic compounds (PAHs). The results indicated that the use of the c-CNTs-PDMS capillary columns improve pyriproxyfen and mainly PAH extraction. Triazines were better extracted by unmodified TRB-35 and modified c-CNTs-PDMSTRB-5. The results showed that the extraction capability of the c-CNT capillary columns depends not only on the polarity of the analytes (as it occurs with PDMS columns) but also on the interactions that the analytes can establish with the immobilized c-CNTs on the PDMS columns. The extraction efficiency has been evaluated on the basis of the preconcentration rate that can be achieved, and, in this sense, the best c-CNTs-PDMS capillary column for each group of compounds can be proposed.

Description: Publication date: Available online 7 August 2015 Source: FEBS Open Bio Author(s): Kimberley J. Anderson, Aaron P. Russell, Victoria C. Foletta The function of the stress-responsive N-myc downstream-regulated gene 2 (NDRG2) in the control of myoblast growth, and the amino acids contributing to its function, are not well characterised. Here, we investigated the effect of increased NDRG2 levels on the proliferation, differentiation and apoptosis in skeletal muscle cells under basal and stress conditions. NDRG2 overexpression increased C2C12 myoblast proliferation and the expression of positive cell cycle regulators, cdk2, cyclin B and cyclin D, and phosphorylation of Rb, while the serine/threonine-deficient NDRG2, 3A-NDRG2, had less effect. The onset of differentiation was enhanced by NDRG2 as determined through the myogenic regulatory factor expression profiles and myocyte fusion index. However, the overall level of differentiation in myotubes was not different. While NDRG2 up-regulated caspase 3/7 activities during differentiation, no increase in apoptosis was measured by TUNEL assay or through cleavage of caspase 3 and PARP proteins. During H 2 O 2 treatment to induce oxidative stress, NDRG2 helped protect against the loss of proliferation and ER stress as measured by GRP78 expression with 3A-NDRG2 displaying less protection. NDRG2 also attenuated apoptosis by reducing cleavage of PARP and caspase 3 and expression of pro-apoptotic Bax while enhancing the pro-survival Bcl-2 and Bcl-xL levels. In contrast, Mcl-1 was not altered, and NDRG2 did not protect against palmitate-induced lipotoxicity. Our findings show that NDRG2 overexpression increases myoblast proliferation and caspase 3/7 activities without increasing overall differentiation. Furthermore, NDRG2 attenuates H 2 O 2 -induced oxidative stress and specific serine and threonine amino acid residues appear to contribute to its function in muscle cells.

Description: Declines in Ogallala aquifer levels used for irrigation has prompted research to identify methods for optimizing water use efficiency (WUE) of cotton (Gossypium hirsutum L). In this experiment, conducted at Lubbock, TX, USA in 2014, our objective was to test two canopy temperature based stress indices, each at two different irrigation trigger set points: the Stress Time (ST) method with irrigation triggers set at 5.5 (ST_5.5) and 8.5 h (ST_8.5) and the Crop Water Stress Index (CWSI) method with irrigation triggers set at 0.3 (CWSI_0.3) and 0.6 (CWSI_0.6). When these irrigation triggers were exceeded on a given day, the crop was deficit irrigated with 5 mm of water via subsurface drip tape. Also included in the experimental design were a well-watered (WW) control irrigated at 110% of potential evapotranspiration and a dry land (DL) treatment that relied on rainfall only. Seasonal crop water use ranged from 353 to 625 mm across these six treatments. As expected, cotton lint yield increased with increasing crop water use but lint yield WUE displayed asignificant (p ≤ 0.05) peak near 3.6 to 3.7 kg ha−1 mm−1 for the ST_5.5 and CWSI_0.3 treatments, respectively. Our results suggest that WUE may be optimized in cotton with less water than that needed for maximum lint yield.

Description: The Saudi Arabia (SA) climate varies greatly, depending on the geography and the season. According to K ppen and Geiger, the climates of SA is “desert climate”. The analysis of the seasonal rainfall detects that spring and winter seasons have the highestrainfall incidence, respectively. Through the summer,small quantities of precipitation are observed, while autumn received more precipitation more than summer season considering the total annual rainfall. In all seasons, the SW area receives rainfall, with a maximum in spring, whereas in the summer season, the NE and NW areas receive very little quantities of precipitation. The Rub Al-Khali (the SE region) is almost totally dry. The maximum amount of annual rainfall does not always happen at the highest elevation. Therefore, the elevation is not the only factor in rainfall distribution.A great inter-annual change in the rainfall over the SA for the period (1978–2009) is observed. In addition, in the same period, a linear decreasing trend is found in the observed rainfall, whilst in the recent past (1994–2009) a statistically significant negative trend is observed. In the Southern part of the Arabian Peninsula (AP) and along the coast of the Red Sea, it is interesting to note that rainfall increased, whilst it decreased over most areas of SA during the 2000–2009 decade, compared to 1980–1989.Statistical and numerical models are used to predict rainfall over Saudi Arabia (SA). The statistical models based on stochastic models of ARIMA and numerical models based on Providing Regional Climates for Impact Studies of Hadley Centre (PRECIS). Climate and its qualitative character and quantified range of possible future changes are investigated. The annual total rainfall decreases in most regions of the SA and only increases in the south. The summertime precipitation will be the highest between other seasons over the southern, the southwestern provinces and Asir mountains, while the wintertime rainfall will remain the lowest.The climate in the SA is instructed by the El Niño Southern Oscillation (ENSO) and other circulations such as centers of high and low pressure, the North Atlantic Oscillation (NAO) and SOI. Strength and oscillation of subtropical jet stream play a big role in pulling hot, dry air masses of SA.

Description: Publication date: Available online 12 August 2015 Source: FEBS Open Bio Author(s): Yang Song, Yonghong Zhang, Haoxiang Jiang, Yanting Zhu, Lu Liu, Wei Feng, Lan Yang, Yibin Wang, Manxiang Li Activation of the Notch3 cascade is involved in the development of pulmonary arterial hypertension by stimulating the proliferation of vascular smooth muscle cells. However, the detailed molecular mechanisms underlying this effect are still unclear. The present study aims to address this issue. We demonstrated that over-expression of intracellular domain of the Notch3 receptor (NICD3) by adenovirus transfection dramatically induced proliferation of primary cultured pulmonary artery smooth muscle cells. This was accompanied with up-regulation of Hes1 protein and down-regulation of p27Kip1protein. More importantly, we observed that prior silencing of Hes1 with siRNA blocked NICD3 over-expression-induced p27Kip1 reduction and cell proliferation. The present study suggests that Hes1 lies downstream of NICD3 and particularly mediates Notch3 signaling-induced proliferation of pulmonary arterial smooth muscle cells by down-regulation of p27Kip1 expression.

Description: Publication date: Available online 11 August 2015 Source: FEBS Open Bio Author(s): Xueying Zhang, Yuanzhe Jin, Xiaohong Zhang, Mingming Lei, Yingzi Lin, Ian L. Megson, Jun Wei, Bo Yu A number of studies have reported an association between increased levels of antibodies against oxidized low-density lipoprotein (oxLDL) and cardiovascular disease, but the anti-oxLDL antibody has not been confirmed to serve as an effective biomarker for prediction of acute myocardial infarction (AMI). Apolipoprotein B 100 (ApoB 100 )-derived peptide fragments generated by proteolytic degradation and aldehyde modification are the major antigens in oxLDL, and so the present work was undertaken to detect circulating IgG for Apo-B 100 -derived peptide antigens. An in-house enzyme-linked immunosorbent assay (ELISA) was developed with eight ApoB 100 -derived peptide antigens (Ag1-Ag8) to detect circulating anti-ApoB 100 IgG levels in 267 patients with AMI and 201 control subjects. Binary logistic regression analysis revealed that circulating IgG for Ag1 was significantly higher in the patient group than the control group ( P 〈0.001) after adjustment for age, gender, smoking, hypertension, diabetes and circulating levels of cholesterol, HDL, LDL, ApoA and ApoB 100 . None of the other seven antigens detected an increase in IgG levels in AMI patients compared with control subjects. Spearman correlation analysis showed no correlation between IgG antibody for Ag1 and clinical characteristics. In conclusion, the linear peptide antigens derived from ApoB 100 may be suitable for the development of an ELISA antibody test for prediction of AMI, although further confirmation is still needed in large-scale clinical studies.

Description: Publication date: Available online 11 August 2015 Source: FEBS Open Bio Author(s): Yu-E. Hao, Dong-Fang He, Rong-Hua Yin, Hui Chen, Jian Wang, Shao-Xia Wang, Yi-Qun Zhan, Chang-Hui Ge, Chang-Yan Li, Miao Yu, Xiao-Ming Yang G protein-coupled receptor kinase interactor 2 (GIT2) is a signaling scaffold protein involved in regulation of cytoskeletal dynamics and the internalization of G protein-coupled receptors (GPCRs). The short-splice form of GIT2 is expressed in peripheral T cells and thymocytes. However, the functions of GIT2 in T cells have not yet been determined. We show that treatment with Con A in a model of polyclonal T-lymphocyte activation resulted in marked inhibitions in the intrahepatic infiltration of inflammatory cells, cytokine response and acute liver failure in Git2 -/- mice. CD4 + T cells from Git2 -/- mice showed significant impairment in proliferation, cytokine production and signal transduction upon TCR-stimulated activation. Our results suggested that GIT2 plays an important role in T-cell function in vivo and in vitro .

Description: In the existing attribute-based encryption (ABE) scheme, the authority (i.e., private key generator (PKG)) is able to calculate and issue any user’s private key, which makes it completely trusted, which severely influences the applications of the ABE scheme. To mitigate this problem, we propose the black box traceable ciphertext policy attribute-based encryption (T-CP-ABE) scheme in which if the PKG re-distributes the users’ private keys for malicious uses, it might be caught and sued. We provide a construction to realize the T-CP-ABE scheme in a black box model. Our scheme is based on the decisional bilinear Diffie-Hellman (DBDH) assumption in the standard model. In our scheme, we employ a pair (ID, S) to identify a user, where ID denotes the identity of a user and S denotes the attribute set associated with her.

Description: Publication date: September 2015 Source: Journal of Hydrology: Regional Studies, Volume 4, Part B Author(s): Alberto Pistocchi, Costanza Calzolari, Francesco Malucelli, Fabrizio Ungaro Study region The plains of Emilia Romagna, Italy. Study focus Urban expansion is among the main causes of increase in flood frequency and intensity in small rural catchments in Europe, and our study region is paradigmatic in this respect. We present here a regional screening-level assessment of soil sealing impacts in terms of increased flood peak discharges and flooding volumes on the secondary drainage network of the plains. We estimate flood peak discharges and flooding volumes through a simple kinematic model with runoff coefficients for the land use of 2008 and 1976. Additionally, we calculate an equivalent compensatory flood detention volume that would enable preserving flood peak discharges as prior to soil sealing (principle of “hydraulic invariance”). The proposed approach is simple and readily applicable to any region facing similar issues, for screening-level assessment of flood hazards over an extended stream network. New hydrological insights for the region The analysis highlights a significant increase in flood hazards throughout the secondary stream network. The impact. Widespread and relatively uniform, is more apparent in smaller catchments and in the case of more permeable soils. This demands retrofitting of the majority of the drainage network and/or significantly higher costs from flooding damages. The analysis suggests that costs of additional flooding after soil sealing may be higher than those of soil sealing impacts compensation through flood detention (hydraulic invariance).

Description: Recently, non-standard Lagrangians have gained a growing importance in theoretical physics and in the theory of non-linear differential equations. However, their formulations and implications in general relativity are still in their infancies despite some advances in contemporary cosmology. The main aim of this paper is to fill the gap. Though non-standard Lagrangians may be defined by a multitude form, in this paper, we considered the exponential type. One basic feature of exponential non-standard Lagrangians concerns the modified Euler-Lagrange equation obtained from the standard variational analysis. Accordingly, when applied to spacetime geometries, one unsurprisingly expects modified geodesic equations. However, when taking into account the time-like paths parameterization constraint, remarkably, it was observed that mutually discrete gravity and discrete spacetime emerge in the theory. Two different independent cases were obtained: A geometrical manifold with new spacetime coordinates augmented by a metric signature change and a geometrical manifold characterized by a discretized spacetime metric. Both cases give raise to Einstein’s field equations yet the gravity is discretized and originated from “spacetime discreteness”. A number of mathematical and physical implications of these results were discussed though this paper and perspectives are given accordingly.

Description: Publication date: Available online 1 August 2015 Source: Dendrochronologia Author(s): Katarina Čufar, Willy Tegel, Maks Merela, Bernd Kromer, Anton Velušček We present dendrochronological dating of Eneolithic pile dwellings on Ljubljansko barje, Slovenia, from the 4th millennium BC, partly included on the UNESCO world heritage list in 2011. Samples of oak ( Quercus sp.) timbers from the posts on which the dwellings were built have been collected over the past 20 years. They have been dendrochronologically cross-dated and (pre) dated by 14 C wiggle-matching. We describe the construction of a 442-year chronology BAR-3330 based on 106 cross-dated tree-ring series of wood from six pile-dwelling sites. Comparison of BAR-3330 with reference chronologies of more than 500 km distant areas north of the Alps showed that it can be teleconnected and dated with a combined German Swiss chronology. The time span of BAR-3330 was defined in this way as 3771–3330 BC. We were thus able to date exactly building activities on the pile dwellings Strojanova voda (SV), Hočevarica (HO), Maharski prekop (MP), Črešnja pri Bistri (CR), Spodnje mostišče (SM) and Stare gmajne (SG), in which early copper metallurgy played an important role. This is the first dendrochronological dating of prehistoric pile dwellings south of the Alps using reference chronologies from the north based on teleconnection. It provides an opportunity to continue filling the spatial and temporal gaps in the absolute chronology of the 4th millennium BC in the area south and south east of the Alps. Graphical abstract

Description: The use of a novel micro pressurized liquid extraction (µPLE) method for the isolation of 16 priority pollutant polycyclic aromatic hydrocarbons (PAHs) from various solid samples is explored. The technique employs rapid heating in a single static extraction mode to remove analytes in a matter of seconds from 5–10 mg samples using only 125 µL of solvent. For example, results show that 30 s extractions with toluene at 200 °C produce respective PAH recovery ranges of 90%–130% and 88%–114% from samples of soil and smoked chicken. Comparatively, solids containing significant amounts of biochar were more challenging to extract from. For instance, when using a pure biochar sample matrix, recoveries for the 16 PAHs range from only 33%–66% after 60 s of extraction with toluene at 200 °C. Overall, these extraction results agree very well with those reported when using conventional methods on similar samples. Therefore, the findings indicate that µPLE can potentially provide an alternative sample preparation method for PAHs that is both very rapid and requires little solvent.

Description: Publication date: Available online 3 August 2015 Source: FEBS Open Bio Author(s): Johan Vande Voorde, Peter Vervaeke, Sandra Liekens, Jan Balzarini Mycoplasmas may colonize tumour tissue in patients. The cytostatic activity of gemcitabine was dramatically decreased in Mycoplasma hyorhinis -infected tumour cell cultures compared with non-infected tumour cell cultures. This mycoplasma-driven drug deamination could be prevented by exogenous administration of the cytidine deaminase (CDA) inhibitor tetrahydrouridine, but also by the natural nucleosides or by a purine nucleoside phosphorylase inhibitor. The M. hyorhinis -encoded CDA Hyor gene was cloned, expressed as a recombinant protein and purified. CDA Hyor was found to be more catalytically active than its human equivalent and efficiently deaminates (inactivates) cytosine-based anticancer drugs. CDA Hyor expression at the tumour site may result in selective drug inactivation and suboptimal therapeutic efficiency.

Description: Digital publishing resources contain a lot of useful and authoritative knowledge. It may be necessary to reorganize the resources by concepts and recommend the related concepts for e-learning. A recommender system is presented in this paper based on the semantic relatedness of concepts computed by texts from digital publishing resources. Firstly, concepts are extracted from encyclopedias. Information in digital publishing resources is then reorganized by concepts. Secondly, concept vectors are generated by skip-gram model and semantic relatedness between concepts is measured according to the concept vectors. As a result, the related concepts and associated information can be recommended to users by the semantic relatedness for learning or reading. History data or users’ preferences data are not needed for recommendation in a specific domain. The technique may not be language-specific. The method shows potential usability for e-learning in a specific domain.

Description: Mangroves are found throughout the tropics, providing critical ecosystem goods and services to coastal communities and supporting rich biodiversity. Globally, mangroves are being rapidly degraded and deforested at rates exceeding loss in many tropical inland forests. Madagascar contains around 2% of the global distribution, >20% of which has been deforested since 1990, primarily from over-harvest for forest products and conversion for agriculture and aquaculture. While historically not prominent, mangrove loss in Madagascar’s Mahajamba Bay is increasing. Here, we focus on Mahajamba Bay, presenting long-term dynamics calculated using United States Geological Survey (USGS) national-level mangrove maps contextualized with socio-economic research and ground observations, and the results of contemporary (circa 2011) mapping of dominant mangrove types. The analysis of the USGS data indicated 1050 hectares (3.8%) lost from 2000 to 2010, which socio-economic research suggests is increasingly driven by commercial timber extraction. Contemporary mapping results permitted stratified sampling based on spectrally distinct and ecologically meaningful mangrove types, allowing for the first-ever vegetation carbon stock estimates for Mahajamba Bay. The overall mean carbon stock across all mangrove classes was estimated to be 100.97 ± 10.49 Mg C ha−1. High stature closed-canopy mangroves had the highest average carbon stock estimate (i.e., 166.82 ± 15.28 Mg C ha−1). These estimates are comparable to other published values in Madagascar and elsewhere in the Western Indian Ocean and demonstrate the ecological variability of Mahajamba Bay’s mangroves and their value towards climate change mitigation.

Description: By recourse to tempered ultradistributions, we show here that the effect of a q-Fourier transform (qFT) is to map equivalence classes of functions into other classes in a one-to-one fashion. This suggests that Tsallis’ q-statistics may revolve around equivalence classes of distributions and not individual ones, as orthodox statistics does. We solve here the qFT’s non-invertibility issue, but discover a problem that remains open.

Description: Aquifers within the Columbia River Basalt Group (CRBG) provide a critical water supply throughout much of the Pacific Northwest of the United States. Increased pumping has resulted in water level declines in this region. Recharge into this aquifer system is generally not well understood. Recent suggestions of probable decades-long droughts in the 21st century add to this problem. We show that geophysical methods can provide useful parameters for improved modeling of aquifers in a primary CRBG aquifer located on the eastern edge of the Columbia Plateau. Groundwater models depend in part on the area, thickness, porosity, storativity, and nature of confinement of this aquifer, most of which are poorly constrained by existing well information and previous stress tests. We have made use of surface gravity measurements, borehole gravity measurements, barometric efficiency estimates, earth tidal response, and earthquake seismology observations to constrain these parameters. We show that the aquifer, despite its persistent drawdown, receives a great deal of recharge annually. Much of the recharge to the aquifer is due to leakage from overlying flows, ultimately tied to precipitation, an important result for future aquifer management in times of sustained drought.

Description: Low flows of the Ilmenau River (1434 km2) in northwest Germany have decreased by about 25% over the last 50 years. In the same period, moderate climate changes have taken place and annual groundwater abstractions for sprinkler irrigation have increased by up to 50 hm3 (million m3), with a strong variation due to the respective prevailing weather conditions. Time-series analyses with multiple regression analysis allow detecting and quantifying different influences on low flows. It is also shown that farmers allocate irrigation water volumes carefully according to seasonal precipitation and temperatures. Decline of groundwater levels in summer and the low flow situation are aggravated by the cumulative effect of higher irrigation in drier years. Groundwater recharge and recovery of the water table have been observed subsequently during the winter season.

Description: Cities’ energy usage accounts for two thirds of global primary energy consumption. Energy efficiency in urban areas is, therefore, one of the most important topics to consider when dealing with urban sustainability. This paper evaluates the goals for increasing energy efficiency and use of renewable energy sources in the areas of transportation, buildings and consumers’ awareness, as stated in the Climate action plan, for the municipality of Eskilstuna, Sweden. The efforts of the municipality to successfully reach their energy efficiency goals, are described in this paper including future perspectives. The results show that although the municipality counts with the advantage of owning and working together with the local housing company and energy provider, in order to reach the established goals, additional strategies need to be considered. For an increased use of renewable energy sources, analysis of rooftops suitable for photovoltaic (PV) installation should be carried out as well as the integration of goals for self-consumption. In the transport field, the city needs to prepare for large-scale electric vehicle (EV) market penetration and to consider different bike or car sharing options. Finally, more specific awareness campaigns are needed to engage the citizens in reducing their energy consumption and living a more sustainable life.

Description: Publication date: Available online 3 August 2015 Source: SoftwareX Author(s): Gianmarco Alberti Correspondence Analysis (CA) is a statistical exploratory technique frequently used in many research fields to graphically visualize the structure of contingency tables. Many programs, both commercial and free, perform CA but none of them as yet provides a visual aid to the interpretation of the results. The ‘CAinterprTools’ package, designed to be used in the free R statistical environment, aims at filling that gap. A novel-to-medium R user has been considered as target. 15 commands enable to easily obtain charts that help (and are relevant to) the interpretation of the CA’s results, freeing the user from the need to inspect and scrutinize tabular CA outputs, and to look up values and statistics on which further calculations are necessary. The package also implements tests to assess the significance of the input table’s total inertia and individual dimensions.

Description: Publication date: July 2015 Source: Global Ecology and Conservation, Volume 4 Author(s): David M.P. Jacoby, John M. Casselman, Vicki Crook, Mari-Beth DeLucia, Hyojin Ahn, Kenzo Kaifu, Tagried Kurwie, Pierre Sasal, Anders M.C. Silfvergrip, Kevin G. Smith, Kazuo Uchida, Alan M. Walker, Matthew J. Gollock With broad distributions, diadromous fishes can be exposed to multiple threats at different stages of development. For the primarily catadromous eels of the family Anguillidae, there is growing international concern for the population abundance and escapement trends of some of these species and yet incomplete knowledge of their remarkable life-histories hampers management and conservation. Anguillids experience a suite of pressures that include habitat loss/modification, migration barriers, pollution, parasitism, exploitation, and fluctuating oceanic conditions that likely have synergistic and regionally variable impacts, even within species. In beginning to redress this rather fragmented picture, we evaluated the extinction risk of these species using the IUCN Red List of Threatened Species Categories and Criteria to infer population-wide trends from catch and monitoring data. Here we consolidate and build upon these species assessments by presenting an overview of the current state of global eel data and conservation, categorising the knowledge gaps and geographic regions where resources are needed and discussing future recommendations to improve our understanding of anguillids. We find stark disparity between the quality and length of data available to assess population trends and conservation priorities in temperate and tropical anguillids. Of the 13 species assessed, four were listed as ‘Threatened’ (Vulnerable, Endangered or Critically Endangered); four were Near Threatened, three were Data Deficient and two were deemed Least Concern. Comparing with other diadromous species, we examine the multiple threats that impact eels during their different life-history stages, highlighting the challenges of applying the Red List Categories and Criteria to geographically-expansive, catadromous and panmictic groups of species.

Description: Publication date: September 2015 Source: Journal of Hydrology: Regional Studies, Volume 4, Part B Author(s): M.A. Sarr, O. Seidou, Y. Tramblay, S. El Adlouni Study region The study considers six precipitation stations located in Senegal, West Africa. Senegal is located in the Sahel, an area that is threatened by climate variability and change. Both droughts and extreme rainfall have been an issue in recent years. Study focus Two different statistical downscaling techniques were applied to the outputs of four regional climate models at six selected precipitation stations in Senegal. First, the delta-change method was applied to the mean annual precipitation as well as the 5, 10, 20, 50 and 100-year return period daily precipitation events. Second, a quantile–quantile transformation (QQ) was used to downscale the monthly distributions of precipitation simulated by regional climate models (RCMs). The 5, 10, 20, 50 and 100-year daily precipitation events were afterward calculated. All extreme events were calculated assuming that maximum annual daily precipitations follow the generalized extreme value (GEV) distribution. The two-sided Kolmogorov–Smirnov (KS) test was finally used to assess the performance of the quantile–quantile transformation as well as the GEV distribution fit for the annual maximum daily precipitation. New hydrological insights for the region Results show that the two downscaling techniques generally agree on the direction of the change when applied to the outputs of same RCM, but some cases lead to very different projections of the direction and magnitude of the change. Projected changes indicate a decline in mean precipitation except for one RCM over one region in Senegal. Projected changes in extreme precipitations are not consistent across stations and return periods. The choice of the downscaling technique has more effect on the estimation of extreme daily precipitations of return period equal or greater than ten years than the choice of the climate models.

Description: Drought is a serious natural hazard with far-reaching impacts including soil damages, economic losses, and threatening the livelihood and health of local residents. The goal of the present work was to monitor the vegetation health across Lebanon in 2014 using remote sensing techniques. Landsat images datasets, with a spatial resolution of 30 m and from different platforms, were used to identify the VCI (Vegetation Condition Index) and TCI (Temperature Condition Index). The VCI was based on the Normalized Difference Vegetation Index (NDVI) datasets. The TCI used land surface temperature (LST) datasets. As a result, the VHI (Vegetation Health Index) was produced and classified into five categories: extreme, severe, moderate, mild, and no drought. The results show practically no extreme drought (~0.27 km2) in the vegetated area in Lebanon during 2014. Moderate to severe drought mainly occurred in the north of Lebanon (i.e., the Amioun region and the plain of Akkar). The Tyr region and the Bekaa valley experienced a low level of drought (mild drought). This approach allows decision makers to monitor, investigate and resolve drought conditions more effectively.

Description: Despite significant advances in watershed science and technology, water availability, water quality, and water related health problems remain a significant worldwide concern [1]. While the concept of watershed-scale management to address these concerns remains intact, most scientists recognize that application of natural science concepts and advanced technologies are not sufficient to adequately address watershed-scale water management issues. There is a significant need for a paradigm shift, i.e., namely increased public interaction and participation in watershed management and decision-making. The effective application of an integrated approach requires developing new scientific concepts on integration of natural and social sciences. In recent years, concepts, such as integrated watershed management and/or holistic approaches to water resource management, have been widely promoted (e.g., [2–6]). [...]

Description: Publication date: Available online 19 August 2015 Source: FEBS Open Bio Author(s): Ajay Srivastava Malignant pleural mesothelioma (MPM) is an aggressive form of thoracic cancer with poor prognosis. While some studies have identified the molecular alterations associated with MPM, little is known about their role in MPM. For example, fragile X mental retardation ( FMR ) gene is up-regulated in MPM but its role in MPM is unknown. Here, utilizing Drosophila genetics, I investigate the possible role FMR may be playing in MPM. I provide evidence which suggests that FMR may contribute to tumorigenesis by up-regulating a matrix metalloprotease (MMP) and by degrading the basement membrane (BM), both important for tumor metastasis. I also demonstrate a novel link between FMR and the JNK pathway and suggest that the effects of FMR in MPM could in part be mediated by up-regulation of the JNK pathway.

Description: Many studies have revealed the cyclicity of past ocean/atmosphere dynamics at a wide range of time scales (from decadal to millennial time scales), based on the spectral analysis of time series of climate proxies obtained from deep sea sediment cores. Among the many techniques available for spectral analysis, the maximum entropy method and the Thomson multitaper approach have frequently been used because of their good statistical properties and high resolution with short time series. The novelty of the present study is that we compared the two methods by according to the performance of their statistical tests to assess the statistical significance of their power spectrum estimates. The statistical significance of maximum entropy estimates was assessed by a random permutation test (Pardo-Igúzquiza and Rodríguez-Tovar, 2000), while the statistical significance of the Thomson multitaper method was assessed by an F-test (Thomson, 1982). We compared the results obtained in a case study using simulated data where the spectral content of the time series was known and in a case study with real data. In both cases the results are similar: while the cycles identified as significant by maximum entropy and the permutation test have a clear physical interpretation, the F-test with the Thomson multitaper estimator tends to find as no significant the peaks in the low frequencies and tends to give as significant more spurious peaks in the middle and high frequencies. Nevertheless, the best strategy is to use both techniques and to use the advantages of each of them.

Description: Publication date: Available online 19 August 2015 Source: Journal of Asian Ceramic Societies Author(s): Satyanarayan Patel, Aditya Chauhan, Rahul Vaish, P. Thomas In the present work, lead-free ferroelectric 0.715Bi 0.5 Na 0.5 TiO 3 -0.065BaTiO 3 -0.22SrTiO 3 (BNT-BT-ST) bulk ceramics with 3BaO-3TiO 2 -B 2 O 3 (BTBO) glass additive were fabricated by conventional solid state reaction route. The effect of glass content on microstructure and energy storage properties of BNT-BT-ST ceramics was investigated. The maximum energy storage of ∼203 kJ/m 3 was achieved for BNT-BT-ST ceramic with addition of 4 wt.% glass. The 4 wt.% glass addition improves energy storage density and energy storage efficiency by ∼15% and ∼52% higher than that of the pure BNT-BT-ST, respectively. The effect of temperature on the energy storage was also estimated. It was observed the temperature has similar effect on energy storage improvement in all compositions. The energy storage density ( U ) dependent scaling behavior on remnant polarization ( P r ), maximum polarization ( P max ), electric field ( E ) and temperature ( T ) was also studied. The results of this study are expected to largely benefit the field of ferroelectric based capacitors in discerning the dependency of U on hysteresis parameters ( P r , P max , and E ) and T .

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): David Granot, Nitsan Lugassi, Jayaram Kottapalli, Gilor Kelly Water is the major factor limiting the growth and development of many land plants, and stomata, composed of two guard cells, are the chief gates controlling plants’ water loss. Many environmental and physiological stimuli control stomatal opening, but they all do so through the regulation of guard-cell osmolarity. Increased guard-cell osmolarity leads to the opening of the stomata and decreased osmolarity causes the stomata to close. The prevailing paradigm is that sugars act as osmoticum in the guard cells, thereby contributing to the opening of the stomata. In contrast, we discovered that sugars close stomata via a non-osmotic mechanism. Furthermore, our results show that the guard cells’ response to sugars is dependent on the sugar-sensing enzyme hexokinase (HXK), which triggers the abscisic acid-signaling pathway within the guard cells, leading to stomatal closure. These findings reveal a feedback-inhibition mechanism that is mediated by a product of photosynthesis, sugar via HXK. HXK in the guard cells senses the sugar level and stimulates stomatal closure, thereby coordinating the sugar level with the rate of transpiration. Increased expression of HXK in guard cells decreases the transpiration rate and improves whole-plant water-use efficiency, with no negative effects on photosynthesis, growth or yield.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): T.S. Mammadov, Sh. Balapour Hydrological regime of large bodies of water, especially in land, is an integral feature of the climatic variability of most territories of their pool and adjacent regions. These waters include the Caspian Sea. 130 rivers flow to the Caspian Sea, which and none emerges, with catchments basin exceeds approximately 10 times the area of the sea itself and equal 3.5 million quadrat.km, can play the role of climate and environmental indicator not only regional but also global change. Currently, the Caspian region is undergoing major changes in the environment that affect the living conditions of the population of the Caspian States. Ecological problems of the Caspian Sea and coastal zones are the result of extensive economic development in the countries of the region. The coastline of the Azerbaijan sector of the Caspian Sea is 825 km. The coastal zone is densely populated and intensively developed. About 40% of the population and the country's industrial facilities are concentrated in the coastal zone. The coast and the bottom are rich with oil and gas. In the region has developed agricultural activities. The coastal area has its own resort and recreational resources. The largess trade port and ferry terminal are in the Caspian Sea in Baku. Socio-economic and ecological problems of the coastal zone of the Caspian Sea have arisen as a result of the exploitation of natural resources of the sea and coastal areas. These problems are further exacerbated by abrupt changes in sea level, play an important role in global climate change. Therefore, it is imperative to examine the causes of sea level change, predicting its long-term fluctuations. Vulnerability assessment of the socio-economic conditions, natural resources of the coastal zone of the Caspian Sea for the forthcoming climate change is an urgent task for the Republic of Azerbaijan.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Robert J. Henry, Parimalan Rangan, Agnelo Furtado Cereals are key foods providing a significant part of the energy (calories) and protein in human diets globally. Cereals are consumed as intact grain products, such as rice, or as ground ingredients, such as wheat in breads, noodles or pasta. The dominance of cereals in human foods makes nutritional attributes of cereals important to the health of human populations. Functional traits influencing the processing or end use quality attributes of cereal based foods are key to human preferences and consumption. Adaptation of cereal crops to variable or changing climates requires that essential quality attributes are retained. Advances in cereal genomics are delivering insights into the molecular basis of nutritional and functional quality traits in cereals that will be critical to retaining essential quality traits. New genetic resources are emerging within the gene pools of the domesticated species. New species 1 adapted to new or different environments may also be options for accelerated domestication to satisfy food demand. Genomic analysis of the diversity of rice genetic resource 2 will provide more options for rice adaptation. New insights into the molecular genetic basis of wheat quality 3 and the influence of the environment on expression of these traits will support the retention of the essential functional properties of wheat during climate adaptation. New cereals for use as whole grain or ground to flour for other food products may be based upon the traditional species such as rice and wheat but may also include new options exploiting genomics tools to allow accelerated domestication of new species.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): William M. Fonta, Safiétou Sanfo, Boubacar Ibrahim, Boubacar Barry Innovative financing arrangements such as index-base crop insurance (IBCI) schemes are increasingly becoming popular in West Africa for managing catastrophic agricultural risks. Recently, an IBCI pilot project was launched in Burkina Faso by PlaNet Guarantee 1 1 A member of the Planet Finance Group and the Global Index Insurance Facility program (GIIF). . However, similar to many existing IBCI schemes in the region, the enrolment rate is still very low. One possible explanation for this is based on the fact that remote sensing data is used as the basis for the design. Although the use of remote sensing data is appealing in many respect, it has several limitations. One major limitation is that it fails to take into account sensitive phases of the crops cycle, which may be more prone to climate and other environmental stresses (Muller, 2014). In this paper, we highlights the importance of using field facts in the design of innovative IBCI schemes in rural Burkina Faso. Farmers’ awareness and perception of climate hazards in relation to crop productivity and their willingness to participate (WTP) in IBCI in South-western Burkina were captured through household surveys and focus group discussions. Empirical findings indicate that farmers are aware of the effects of climate hazard on farm productivity and consider mid-season dry spells, during sowing, flowering and ripening depending on the crop type, as the most significant climate risk affecting local crop productivity. Specifically, 98% of the sampled farmers are willing to insure maize, cotton and sorghum, but only if the most sensitive periods of these crops to dry spell are taken into account in designing the crop insurance contracts. Furthermore, Probit regression analysis indicates that the probability to participate increases with years of farming experience, past experience of climate hazards, educational attainment of household head and insecurity to climate hazard, and decreases with farmer's age and household size.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Samuel I. Haruna, Nsalambi V. Nkongolo Cover crops have been known to reduce soil erosion, among other benefits, and increase water infiltration, organic matter and soil microbial activity. This study was conducted at Lincoln University's Freeman farm during 2011 and 2012 to assess the effects of cover crop management on soil physical and biological properties. The soil of the experimental site was a Waldron silt loam soil (Fine, smectitic, calcareous, mesic Aeric Fluvaquents). The field was 4.05 ha in size and subdivided into 48 plots, each measuring 12.2 m x 21.3 m. The cropping pattern for the plots was a corn ( Zea mays L.)/soybean ( Glycine max ) rotation. The cover crop of choice was cereal rye ( Secale cereale ). Half of the total plots had cover crop management while the other half had no-cover crop. Soil samples were collected at four depths; 0-10, 10-20, 20-40 and 40-60 cm. Samples were oven dried at 105 o C for 72 h for soil physical properties analysis. Air dried soil samples were also sent to a commercial laboratory for analysis of soil biological properties. Results showed a significant effect (p〈 0.05) of cover crop on the selected soil physical and biological properties. A 3.5% decrease was also observed in soil bulk density in cover crop plots as compared with no-cover crop plots. The carbon to nitrogen (C/N) ratio decreased with increasing sampling depth for the first three depths and increased slightly in the fourth depth (p〈0.05). C/N ratio also showed a 5.6% increase in no-cover crop plots as compared with cover crop plots. The cover crop used in this study was capable of significantly improving soil physical and biological properties.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Amélie C.M. Gaudin, Tor Tolhurst, Alan Ker, Ralph Martin, Willima Deen A key strategy for climate change adaptation in the rain-fed northern Corn Belt is to decrease cropping system vulnerability to changes in precipitation patterns by building resilience. Using 50- year of county level yield and environmental data from Iowa and Ontario, we first demonstrate that sensitivity of corn yield to precipitation, particularly in July and August, has increased over the past five decades despite no changes in precipitation patterns. This can be attributed to steady improvement in corn yield potential and so plant water demand since the mid-20th century and removal of non-water constraints to crop production. Such vulnerability of corn-based cropping systems to water limitations is of increasing concern as climate change models predict higher summer temperatures and year-to-year variations in precipitations in this region. As suggested in the ecology literature, increasing agroecosystem temporal and spacial diversity is one of the key management strategies to deal with impending weather variability. Using yield and environmental data from a 30-year long-term rotation and tillage trial in Ontario, we show that diversification of short corn-based rotations using small grains and forage crops increases corn yield stability and resilience to both limiting and excess soil moisture1. We also demonstrate the importance of conservation tillage and measured the impact of rotation and tillage history on plants ability to access water resources, plant available soil water and their combined effects on timing of physiological water stress and grain yield when drought occurs at reproductive stages. Our results emphasize the growing importance of developing strategies for managing soil moisture in rain-fed regions and the significance of agroecological approaches to develop hardy agricultural systems and protect food and feed production against the upcoming extreme weather events.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): A. Singh, L. Billa, M.B. Sokoto, A. Lado, M.I. Abdoul-Aziz, S. Singh Changing climate presents one of the most challenging threats to food crop production throughout the agro-ecologies. Annual food crops on which we rely most on are sensitive to temperature and precipitation change as it not only affects the temperature and moisture of the surroundings but affects the levels in soils as well. While climate change will have global impacts on crop production, regional variation will be play significant role in tackling climate change problem. This study examines 10-30 years data on the regional variation in temperature and precipitation from one location each in Arid and semi-Arid climate, one location in Dry sub-Humid climate and six locations in the Tropical Forest climate. The data collected were critically analysed using 5-10 years moving average plots, linear trend analysis and t-test using SPSS ® software. Depending on the location, results showed significant (P〈0.05) increase in temperature ranging from 0.015-0.045 o C. Increase was mostly in the last 5-10 years compared to 1983-1990. Also, this increment was higher in semi-Arid & Dry-humid climate (0.044-0.087 o C) than in Tropical rainforest where the increase was 0.015 o C. No significant (P>0.05) increase in temperature was observed in the Arid climate in the last 10 years. In tropical rainforest climate, increase in temperature (0.044 o C) was higher in the metropolis area than the countryside. From the locations examined in this study, there was no significant change in the precipitation pattern in the last 10-30 years. Simple plots overlaying temperature changes on crop production data revealed mixed response. Groundnut ( Arachis hypogea ), Bambara groundnut ( Vigna subterannea ) and hungry rice ( Digeteria exilis ) showed tolerance to increased temperatures in the Semi-Arid tropics. Rice ( Oryza sativa ) in Tropical Malaysia showed increased in yield with temperature. What may be important to note here is the resilience of the genotypes to temperature changes so that these genotypes could be further studied for adaptation to climate change. No doubt that there is evidence of increase in temperature, regional variations will exist and will play an important role in mitigating climate change.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Abdullah A. Jaradat Eleven genotypes of Carthamus tinctorius, Cicer arietinum, Glycine max, Triticum spp. [ aestivum and durum ], and Zea mays , respectively producing oil, proteins, oil-protein, carbohydrates-protein, and carbohydras as the main biochemical seed components (Products) were subjected to four combinations of abiotic stresses imposed by manipulating planting dates and population densities (Management). Each genotype was planted in three replicates in RCBD on the same land area for three consecutive years as an additional edaphic stress [Phase I], followed by three more years in a crop rotation [Phase II]. Annually, three random plants per genotype and replicate were sampled at vegetative and physiological maturity stages. All samples were assayed for 10 nutrients, using LECO analyzer and ICP instrument. Temporal variation in nutrient density and stability were estimated using several multivariate statistical methods. Validation coefficients of determination [Q2] increased steadily from 25 [Phase I] to 53% [Phase II]. Invariably, Sulfur, Phosphorus, Zinc, and Copper, in decreasing order, were most important in determining the amount of explained variance. Abiotic stress significantly increased nutrient densities in 32, decreased it in 46, and did not affect it in 22% of the 50 nutrient-Product combinations. Temporal variation of nutrient densities decreased in 48, increased in 34, and remained stable in 18% of these nutrient-Product combinations. Carbon:Nitrogen ratio, as covariate, impacted nutrient densities, and stability of all nutrients; increased in carbohydrates, protein, and oil-protein; and decreased in oil. Nutrient densities averaged over Products discriminated between Phase I and Phase II [91.1 vs. 96.0% correct classification]. Discrimination between Products decreased from 73.5% in Phase I to 62.5% in Phase II. Carbohydrates, oil, carbohydrates-protein, and oil-protein, in decreasing order, exhibited the largest misclassification. Largest variation in nutrient densities was explained by Year x Product (Phase) [10-73%], followed by Year x Genotype (Products x Phase) [9-25%], thus illustrating the dynamic nutrient response to abiotic stress.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Rebecca J. Thistlethwaite, Daniel K.Y. Tan, Thomas N. Buckley, Richard M. Trethowan Extreme heat waves continue to occur across Australia, particularly in the Northern regions of New South Wales where temperatures exceed 35 °C throughout summer. Wheat germplasm with superior tolerance to short but extreme heat waves (e.g., 1-3 days exceeding 35 °C) compared to currently available wheat varieties was identified. Combinations of experimental approaches were used to impose heat stress in the greenhouse and the field. Two replicates of 120 genotypes were planted across four consecutive sowing dates with later sowings experiencing progressively higher temperatures. Using in-field heat chambers, high temperature stress was applied to a subset of ten genotypes thought to have a superior tolerance to heat at anthesis. To compensate for random seasonal weather effects, these experiments were concurrently replicated by imposing heat stress in a greenhouse facility. This provided side-by-side comparison of a range of commercial genotypes grown widely in northern NSW. Yield and quality stability were the primary measures of temperature tolerance. Other variables that discriminated for response to high temperature included seed set, seed size and seed yield.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Helder Fraga, Aureliano C. Malheiro, José Moutinho-Pereira, João A. Santos Introduction Viticulture is a climate sensitive crop since optimum growth requirements are generally restricted by atmospheric conditions. Given the projected climate change scenarios, grapevine growth may be further challenged in the future. Owing to the importance of the vitivinicultural sector in Europe, the assessment of future climatic impacts in viticulture is of utmost relevance for the winemaking sector. Methods This study was conducted using a 9-member ensemble of regional climate models under the International Panel on Climate Change 4.5 and 8.5 representative concentration pathways (RCPs) until 2050. To evaluate the current and future optimum condition for quality winemaking, the growing degree day is calculated over Europe at a very high spatial resolution (〈1 km). Changes in ensemble means are analysed and the climate signal is isolated for each future RCP. Results As expected, a significant warming is anticipated over Europe in both future scenarios, strengthened in RCP8.5. Over southern Europe, the projected warming is expected to have detrimental impacts on winegrape development and quality, requiring additional measures to deal with heat stress. Over central Europe, growing conditions are expected to change into warmer growing seasons, which may result in changes in wine typicity. Conversely, over northern Europe, the warmer climate may prove more suitable for winegrape growth, leading to a northward shift of the current optimum growth conditions for grapevines. Discussion Climate change is thus expected to impose new challenges for the European winemaking sector. Adaptation measures need to be adequately and timely planned in order to cope with climate change impacts on viticulture. Over the Mediterranean-like climatic regions, these measures may be required for the future suitability of this crop and adaptation to harsh environmental conditions. Over the Atlantic/Continental regions, despite the general increase in suitability, additional measures are also required to adapt to the new climatic conditions.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): P.V.V. Prasad, S.V.K. Jagadish The increased probability of occurrence of more intense and frequent heat stress episodes and extended warmer nights in the future are major challenges towards sustaining agricultural production. Cereals, millets and oil seed crops respond differently to increasing temperature at different growth and developmental stages but are highly susceptible to heat stress during the gametogenesis and the flowering stages. Interestingly, the duration of stress exposure induces differential responses i.e. season-long exposure to high-temperature and short episodes of heat stress coinciding with reproductive processes. Season-long high-temperature stress decreased biomass production, seed number, individual seed weight and yield of all grain crops. Short duration heat stress coinciding with either gametogenesis or anthesis leads to negative impact of seed-set, while stress exposure at post-anthesis stages decreased seed filling duration leading to decreased seed weight. Based on extensive research we have identified temperature thresholds across field crops and quantified the above changes. Impact of heat stress on pollen production, pollen viability, pollen lipid profiles, alternations in the pollen and stigmatic surface, pollen reactive oxygen species production, their membrane damage etc., differentiating contrasting cultivars across different field crops will be highlighted. In addition, considering the differential rate of increase in minimum night temperature, different physiological routes inducing yield losses under high day and high night temperature will be presented. Genetic variability across field crops in response to heat stress and more interestingly options from wild species of wheat (higher heat tolerance at flowering), rice (heat escape through early morning flowering) and their usefulness in heat stress tolerance breeding will be an interesting addition to our talk. The current progress achieved, opportunities available, unaddressed challenges and future direction of research that could help in crop improvement to sustain global food production under future hotter climates will be discussed.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Hideki Ueyama, Norihiro Hoshi, Hirohisa Nesumi Rural districts located in mountainous or hilly regions are not only important in terms of food production but are also where civilization, culture and spirituality originated and are preserved. The policies supporting the sustainable development of agriculture in such regions are important in many countries. These policies will gain importance in the face of climate change because the complex terrain results in small and widely distributed farm lands with limited productivity, and these will be the first to be negatively affected. Many attempts have been made to use various meteorological conditions to increase the productivity of agriculture in the hilly and mountainous terrains of Japan. However, most of the meteorological data collected has not been fully used. We proposed that to optimize agricultural output from the mountainous and hilly regions, it is necessary to acquire precise meteorological data with three characteristics: precise position estimates, substantial data collection, and the availability of continuous data covering the past, present and future. We have developed a support system for the management of a database of precise meteorological data, including air temperature, humidity, solar radiation, precipitation and reference evapotranspiration. Data in the database have been developed to estimate data, having a few tens of meters resolution, based on a nearby existing observation station and its data: past, present and future. This system, with its precision meteorological data, should make citrus cultivation more profitable because Japanese citrus orchards are located in hilly areas, and the gradient of ∼45% of the orchard area has over a 15 degree slope. We expect that this system will contribute to developing advanced and flexible management systems of cultivation that respond to meteorological fluctuations. We will present the support system under the development of citrus orchards.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Fariba Shafiei Koij, Jalal Saba Beans due to having a high protein are important in food regime in developing countries including Iran. Beans are planted on five continents of the world and its cultivated area is 36148114 hectare all over the world. The cultivated area of this crop in Iran is 98000 hectare and its production is 253000 ton (FAO, 2013). One of the most important methods to increase quality and quantity of agricultural products is plant breeding. Selection is the basic stage of plant breeding and applying it required evaluating available germplasm. The fundamental method to develop variation for selection is crossing genotypes where selecting the parents may be very important and existing genetic differences between the crossing parents be required. Grouping the genotypes can make the opportunity to the breeder so that can select appropriate parents for crossing. The aims of the present research were to group 45 lines of white bean and to determine their important traits in order to explain their differences. To do this the lines evaluated in a randomized complete block design with three replications on the research farm of University of Zanjan. Protein content and nine agronomic traits were evaluated. Genotypes divided in 4 groups by cluster analysis among which the genotypes of the fourth group were superior in terms of all traits. Based on principal component analysis the first three principal components were selected that were justified 70% of total variations. Grouping of lines in biplots had much more conformity with the results from dendrogram and showed more importance of the first principal component, which justifies much of total variance. As a result, grain yield, biomass, number of seed per pod, and stem diameter can be recommended as the most important traits to select lines of white bean.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): José Filipe T. Ganança, José Gregório F. Freitas, Humberto Gil M. Nóbrega, Vanessa Rodrigues, Gonçalo Antunes, Miguel Rodrigues, Miguel Ângelo A. Pinheiro de Carvalho, Vincent Lebot Taro ( Colocasia esculenta (L.) Schott) is a vegetative propagated tropical root occupying the 9th position among world food crops. Taro is an important staple food for many local populations of Asia and Africa. The crop is dependent of wet and highly irrigated growth conditions. Under the scenario of undergoing climatic changes, is estimated that taro production could decrease in the next 30 years as result of drought constraints. The project EU Aid Taro, DCI-Food/2009/45 “adapting propagated crops to climatic and commercial changes” aims to study ways to adapt this clonally propagated root to these climatic changes. A set of drought stress assays were realized at ISOPlexis Genebank (Madeira University), using local taro cultivars (Macaronesian taro genepool) and elite cultivars and seedlings provided by the Secretariat of the Pacific Community (SPC). The 1st pilot assay was realized in a small greenhouse, included 3 local cultivars and aimed to modelate the wattering regimes and their influence on crop growth. The 2nd full assay was realized in two greenhouses, during a full plant growth cycle. Thirty four taro cultivars, 15 elite lines provided by SPC, and 19 local cultivars, from Madeira (10), Canary Islands (6), Azores (2), and Cyprus (1) were screened. Plants were individually cultivated in pots under 2 contrasting wattering regimes, high (0,36 liters/day) and low (0,18 liters/day). Data for 14 morpho-agronomic traits, biomass and yield parameters were collected from 5 plants for each cultivar, per treatment. Preliminary results show significant differences in the ability to cope with drought stress among elite and local varieties. A classification of cultivars according to their relative drougth tolerance was made. The most resilient of these varieties will be subject to a new complete assay, in order to better assess their level of tolerance and recommend for breeding programs in Asian countries.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Fereshteh Pourazari, Giulia Vico, Birgitta Båth, Martin Weih One of the most important resources within agriculture is nitrogen (N), and depletion of N resources is an important element in the evaluation of sustainability in agriculture. Therefore, identifying crops with high nitrogen use efficiency (NUE) is important for the sustainability of the system. In an energy crop context, sustainability in crop production could aim at enhanced energy output with maintained or reduced depletion of N resources. Crops with different photosynthetic pathways (C 3 vs. C 4 ) and life histories (perennials vs. annuals) are expected to differ in NUE and also energy harvest per unit N lost from the system. The aim is to characterize the growth, NUE and energy output per unit N lost for three common crops frequently used for energy; maize, winter wheat and perennial grassland (ley). These crops differ in photosynthetic and life history strategies. Above ground biomass of wheat, grassland ley and maize was sampled within a long-term experiment in Central Sweden. The experiment has a split-plot design with four replicates. Four aboveground harvests were conducted during the growth period and plant N contents were determined. Biomass growth, yield (above ground biomass in ley and maize, grain biomass in wheat) and some functional traits were assessed, and the NUE components N uptake efficiency, yield-specific N efficiency and yield N concentration were calculated according to Weih, et al. (2011). Energy output per N lost with the harvested product was calculated assuming crop-specific higher heating values for biofuel use. The N uptake efficiency and yield-specific N efficiency were higher in maize than wheat and ley. The yield N concentration was higher in the perennial ley than the annual crops, and lowest in maize. Energy output per N lost in the harvested product was greater in maize compared to wheat and ley. The results are discussed in a agricultural sustainability perspective.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Maendy Fritz, Anja Hartmann Perennial crops can be used as renewable resource but only rare information concerning their site-specific biomass production are available. Also, their yield stability as well as the quality of the produced biomass, whether for energetic or material utilisation, is relatively unknown. Perennial crops offer several ecological benefits such as protection of soil and waterbodies, long flowering and cover for wild animals during winter. Most of these crops have a high yield potential and are – after planting and establishing is completed – labour efficient to cultivate. Concerning climate change and the presumed higher risk of extreme weather conditions, perennial crops might be a good supplement to common annual crops. The aim of a Bavarian project, started 2014, is to evaluate several perennial crops at six Bavarian sites with varying soils and climatic conditions. Cup plant ( Silphium perfoliatum ), sida ( Sida hermaphrodita ), tall wheatgrass ( Agropyron elongatum ), switchgrass ( Panicum virgatum ) and miscanthus ( Miscanthus x giganteus ) are cultivated in field trials in 100 m 2 plots and four replications. The annual crops maize and rye are similarly grown as references. Besides plant development and yield measurement, the potential methane yield and/or fuel properties will be determined to assess the quality and suitability of these crops for different usages. Trials concerning cultivation techniques and weed control complement the agricultural investigations. Ecological aspects of perennial cropping will be evaluated by measuring carbon levels in the soil as well as stocking rates of earthworms used as indicator for changes in soil biota as a result of perennial crop cultivation. Yield and quality results from foregoing trials for the various crops and first data of the now started field trials will be presented. The data and experiences will allow detailed consulting of farmers who are interested in increasing the biodiversity in their fields and minimising their climate-caused production risks.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Abdullah A. Jaradat Macro- and micro-nutrients estimated in leaves, stems and seed of eleven genotypes of five physiologically diverse crop species (chickpeas, corn, safflower, soybean, and wheat, respectively producing protein, carbohydrates, oil, protein-oil, and carbohydrates-protein, as main seed storage macromolecules) were used in assessing species and genotypic responses to multiple, long-term abiotic stresses. Crops were subjected to two phases, three years each, of multiple abiotic stresses by manipulating length of the growing season and population density under typical management practices of each crop in the upper Midwest, USA. In Phase II, crops were rotated to release the additional edaphic stress of no-rotation in Phase I. Nutrient densities were estimated using LECO analyzer (Carbon and Nitrogen) or ICP instrument (Calcium, Copper, Iron, Manganese, Magnesium, Phosphorus, Potassium, Sulfur and Zinc). Comparisons of functional relationships among nutrients were based on statistics (α, β, and R2) derived from 1000-permutations using reduced major axis (RMA) regression analyses. Genotypic differences in nutrients functional relationships were modulated, in decreasing order, by Carbon:Nitrogen ratio, stress treatments, and storage macromolecules. Comparisons between the β statistics for each nutrient in Phases I and II suggested that differences in nutrient functional relationships between crop species were significantly larger than differences between genotypes within species. Nitrogen, rather than Carbon content, followed by plant density, but not short growing season, influenced some (Phosphorus, Potassium, Sulfur, and Zinc) nutrient relationships and their allocations to leaves, stems and seed of crop species. Functional relationships between Copper, Iron, Sulfur and Zinc, at the seed storage macromolecules level in Phase I and II indicated that oil and protein producing crop species are more prone to larger adverse effects of abiotic stresses than those producing carbohydrates alone or in combination with protein. A thorough understanding of these relationships is critical for screening genetic diversity and designing nutritionally-balanced crop genotypes under abiotic stress.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Jana Zinkernagel, Nadine Schmidt, Katrin Kahlen Today, vegetable production is already limited by local climate and water availability. Knowledge has to be generated for crop management adapted to climate change. Evaluating potential consequences for vegetable production in a model growing region is the scope of this investigation. Simulations of climate conditions and in that respect the use of different climate models allow estimating a broad range of scenarios affecting cultivation. Analysis focuses on climatic parameters relevant for future irrigation demand. By estimating the climatic water balance (CWB), seasonal water demand and crop specific vulnerabilities to water deficit can be revealed. These objectives require knowledge of potential changes in time and duration of vegetable cultivation, gained from analysing thermal growing season (TGS). A broad range of scenarios was achieved for the German region “Hessian Reed” by applying A1B scenario and using the regional models WETTREG 2010, C-CLM and REMO, driven by the global models ECHAM5 and HadCM3, respectively. Simulations cover the period from 1971 to 2100; the validation is based on the period 1971 to 2000. For onion, crop specific CWB was calculated by employing crop coefficients of the Geisenheim Irrigation Scheduling, following FAO-56. TGS was derived from simulated onset and duration of plant growth stages based on temperature sum. Precipitation pattern vary between the models, but still follow a similar trend to higher evaporative demand. CWB decreases by 3 mm/d (WETTREG) and 2 mm/d (C-CLM), respectively. Maximum duration of dry periods increases from 33 to 53 d and occur more frequently from 343 to 457 times for REMO, representing maxima compared to C-CLM and WETTREG. TGS shifts from DOY 60 to 21 for sowing date, but extends from DOY 183 to 208 due to lower temperature (C-CLM). In contrast to the expected increasing irrigation demand in case of unchanged TGS, CWB becomes less negative with TGS-shift.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): S.C. Bertolli, G.M. Souza The strategy adopted by plants for exploit environments with low moisture may depend on the neighbor's identity. Roots of the plants have the ability to discriminate self (roots of the same plant) of non-self (roots of other plants), even without physical contact and while other plants are genetically identical. We investigated if there is any change in the plant behavior that self ( Sd ) and non-self ( NSd ) discriminate in situations of water limitation, and whether such behavior can be varied in individuals more or less kin. It was used two Eucalyptus urophylla groups: clones (high kinship- Hk ); and population (low kinship- Lk ). Split-root plants were planted so that each pot contained either two roots of the same plant ( Sd ) or of two different plants ( NSd ). The plants were subjected to full irrigation ( Irr ) or water deficit ( WD ) for thirty days, and biomass parameters of were evaluated. Plants of both groups and both discrimination conditions reduced their total dry mass ( DMt ) under WD . In Hk group, the WD increased the root/shoot ratio (23% compared to irrigated plants) by NSd , indicating that even when plants show a reduction in growth mediated by lack of water, there is an increase in biomass allocation to the roots when the plants interact each plants. In Hk group, the NSd reduced the dry mass ( DMr ) and volume ( Vr ) of roots only in Irr . In Lk group, DMt , DMr , Vr , and leaf area were reduced in NSd under Irr condition, whereas under WD , the NSd reduced just the specific leaf area (21% compared to Sd ), indicating a change in biomass allocation to leaves. The results suggest that the discrimination is more evident in population group and in non-stressful environments, and although not so pronounced in situations of WD , these responses also occur as observed in Lk plants.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): I. McFarlane, J.R. Park Break crops and multi-crop rotations are common in arable farm management, and the soil quality inherited from a previous crop is one of the parameters that determine the gross margin that is achieved with a given crop from a given parcel of land. In previous work we developed a dynamic economic model to calculate the potential yield and gross margin of a set of crops grown in a selection of typical rotation scenarios, and we reported use of the model to calculate coexistence costs for GM maize grown in a crop rotation. The model predicts economic effects of pest and weed pressures in monthly time steps. Validation of the model in respect of specific traits is proceeding as data from trials with novel crop varieties is published. Alongside this aspect of the validation process, we are able to incorporate data representing the economic impact of abiotic stresses on conventional crops, and then use the model to predict the cumulative gross margin achievable from a sequence of conventional crops grown at varying levels of abiotic stress. We report new progress with this aspect of model validation. In this paper, we report the further development of the model to take account of abiotic stress arising from drought, flood, heat or frost; such stresses being introduced in addition to variable pest and weed pressure. The main purpose is to assess the economic incentive for arable farmers to adopt novel crop varieties having multiple ‘stacked’ traits introduced by means of various biotechnological tools available to crop breeders.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Martin Erbs, Remy Manderscheid, Adam Luig, Henning Kage, Hans-Joachim Weigel Among the parameters of global climate change the increases of the atmospheric CO 2 concentration and of temperature, both of which affect plant growth and development, are of prime importance. However, with respect to the importance of these climate changes for global food security little is known on the possible interactions of these prominent changes on crop growth. This is holds also true for anticipated increases in mean temperatures as well as for short-term high temperature events (i.e. heat stress). Form previous studies it is known that wheat is particularly sensitive to temperatures of 30 °C and above during the anthesis and early grain filling. Short-term heat stress may decrease yield by impairing grain set. Higher average temperatures during grain filling may influence yield by reducing grain filling duration. If and to what extend elevated CO 2 may affect crop responses to heat stresses remains a matter of debate, particularly because of a lack of suitable experiments. While there are a few model projections addressing the interactive impacts of these climate change elements on crops, these models nearly exclusively rely on data from non-field experiments. At the Thünen-Institute of Biodiversity (Braunschweig, Germany) for the first time field experiments with winter wheat were conducted, in which elevated canopy temperature treatments (free-air temperature enrichment = FATE) were applied to the plants in 2013 and combined with free-air CO 2 enrichment (FACE, 600 μmol mol -1 ) in 2014 and 2015. The temperature treatments comprised 1.) at anthesis a short-term heat stress treatment (5 or 12 h d -1 for 5 days, maximum increase up to +6 °C) and 2.) during grain filling a long-term increase (24 h d -1 for 28 days, average increase up to +3 °C). Here we present details of the experimental set-up, the system performance and among others results of heat stress effects at anthesis on yield components.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): Renan Le Roux, Etienne Neethling, Cornelis Van Leeuwen, Laure De Resseguier, Malika Madelin, Cyril Bonnefoy, Gerard Barbeau, Hervé Quenol Firstly this study illustrate regional climate projections for several European vineyards areas at mean- and long-term. Secondly, from a network of data loggers, a modeling approach at local scale is presented to reveal climate variability as an important component to reduce local uncertainties and its impacts on plant behaviour. 3 First part of study concerns all of ADVICLIM vineyards sites. Downscaled Regional Euro-Cordex models are used to map average temperatures and growing degree days differences between 1970-2000, 2020-2050 and 2050-2070. Simulated data from two scenarios are used to show the potential impact of climate change on grapevine behavior at regional scale. Second part of study draws on data obtained from two sites: Saint-Emilion and Coteaux du Layon, France. Daily temperature data are used to quantify and model local climate variability in relation with local environmental features .1,2 At regional scale, a large variability of climate appears inter- and intrasites Projected directional climate change demonstrate significant warming and changes in rainfall patterns, however results reveal great uncertainties as a result of natural climate variability, climate models and socio-economic scenarios. Local scale study show important variability of temperature and growing degree days over very short distances which is related to grapevine phenology and productivity. Regional models are not accurate enough to take into account local climate variability. For this reason Working with nested scales (local and regional) allow to better frame adaptations options to future climate change.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 29 Author(s): L.A. Avila, M.G. Marchezan, E.R. Camargo, D. Agostinetto, C.E. Schaedler, B.S. Moraes, A.C. Langaro, C. Oliveira Extreme rainfall events are expected to be more frequent with the climate change, increasing the chances of prolonged flooding in rice producing areas. Therefore, it is important to understand the response of rice plants to flooding events that would result in plant submersion and it is interaction with herbicide applications. To achieve the objectives two studies were conducted in Southern Brazil (Pelotas, RS). Experiment 1 was conducted with ten rice cultivars and six submersion periods (one, five, seven, 15 e 20 days of plant submersion plus a check treatment. In the check treatment plants were keep under a flooding regime (water layer of 10 cm).). Experiment 2 was conducted with two cultivars, two water regimes (flooded and submerged) and six herbicides (clomazone, bispyribac sodium, penoxsulam, imazethapyr + imazapic, imazapyr + imazapic and an untreated check). Shoot dry matter, chlorophyll levels, total carotenoids, total phenolic content, levels of hydrogen peroxide and activity of catalase (CAT), ascorbate peroxidase (APX) and superoxide dismutase (SOD) were assessed in plant samples. The results observed in the first experiment showed that cultivars Puitá INTA CL, IRGA 417 and IRGA 422 CL were the most tolerant to submergence. The most susceptible cultivars were IRGA 424, BRS Querência, EPAGRI 108 and BRS Taim. In general, the cultivar Puitá INTA CL has lower oxidative damage than BRS Querência when submerged. In order to cope with excessive free radicals, BRS Querência demonstrated higher activity of SOD, APX and CAT enzymes compared to cultivar Puitá INTA CL. Imidazolinone formulated mixtures of herbicides imazethapyr + imazapic and imazapyr + imazapic caused greater reduction in total chlorophyll content and carotenoids.

Description: Publication date: Available online 20 August 2015 Source: Cell Reports Author(s): Bettina Schreiner, Elisa Romanelli, Pawel Liberski, Barbara Ingold-Heppner, Bettina Sobottka-Brillout, Tom Hartwig, Vijay Chandrasekar, Helge Johannssen, Hanns Ulrich Zeilhofer, Adriano Aguzzi, Frank Heppner, Martin Kerschensteiner, Burkhard Becher Although the importance of reactive astrocytes during CNS pathology is well established, the function of astroglia in adult CNS homeostasis is less well understood. With the use of conditional, astrocyte-restricted protein synthesis termination, we found that selective paralysis of GFAP + astrocytes in vivo led to rapid neuronal cell loss and severe motor deficits. This occurred while structural astroglial support still persisted and in the absence of any major microvascular damage. Whereas loss of astrocyte function did lead to microglial activation, this had no impact on the neuronal loss and clinical decline. Neuronal injury was caused by oxidative stress resulting from the reduced redox scavenging capability of dysfunctional astrocytes and could be prevented by the in vivo treatment with scavengers of reactive oxygen and nitrogen species (ROS/RNS). Our results suggest that the subpopulation of GFAP + astrocytes maintain neuronal health by controlling redox homeostasis in the adult CNS. Graphical abstract Teaser Schreiner et al. examine the functional contribution of astrocytes to tissue homeostasis in the adult CNS and identify the redox-scavenging capacity of GFAP + astrocytes as a key factor for neuronal health in vivo. The importance of the metabolic integrity of the glia-neuron interface highlights potential therapies for the treatment of neurodegenerative diseases.

Description: Publication date: Available online 20 August 2015 Source: Cell Reports Author(s): Priya Srikanth, Karam Han, Dana G. Callahan, Eugenia Makovkina, Christina R. Muratore, Matthew A. Lalli, Honglin Zhou, Justin D. Boyd, Kenneth S. Kosik, Dennis J. Selkoe, Tracy L. Young-Pearse Genetic and clinical association studies have identified disrupted in schizophrenia 1 ( DISC1 ) as a candidate risk gene for major mental illness. DISC1 is interrupted by a balanced chr(1;11) translocation in a Scottish family in which the translocation predisposes to psychiatric disorders. We investigate the consequences of DISC1 interruption in human neural cells using TALENs or CRISPR-Cas9 to target the DISC1 locus. We show that disruption of DISC1 near the site of the translocation results in decreased DISC1 protein levels because of nonsense-mediated decay of long splice variants. This results in an increased level of canonical Wnt signaling in neural progenitor cells and altered expression of fate markers such as Foxg1 and Tbr2. These gene expression changes are rescued by antagonizing Wnt signaling in a critical developmental window, supporting the hypothesis that DISC1-dependent suppression of basal Wnt signaling influences the distribution of cell types generated during cortical development. Graphical abstract Teaser Srikanth et al. report the generation of isogenic hiPSC lines with engineered mutations in two locations within the DISC1 gene. This disease-relevant disruption shows a loss of long isoforms, which, in turn, affects neural progenitor cell proliferation, baseline WNT signaling, and the expression of NPC fate markers such as FoxG1 and Tbr2.

Description: Publication date: Available online 20 August 2015 Source: Cell Reports Author(s): Ivana Vonkova, Antoine-Emmanuel Saliba, Samy Deghou, Kanchan Anand, Stefano Ceschia, Tobias Doerks, Augustinus Galih, Karl G. Kugler, Kenji Maeda, Vladimir Rybin, Vera van Noort, Jan Ellenberg, Peer Bork, Anne-Claude Gavin Many cellular processes involve the recruitment of proteins to specific membranes, which are decorated with distinctive lipids that act as docking sites. The phosphoinositides form signaling hubs, and we examine mechanisms underlying recruitment. We applied a physiological, quantitative, liposome microarray-based assay to measure the membrane-binding properties of 91 pleckstrin homology (PH) domains, the most common phosphoinositide-binding target. 10,514 experiments quantified the role of phosphoinositides in membrane recruitment. For most domains examined, the observed binding specificity implied cooperativity with additional signaling lipids. Analyses of PH domains with similar lipid-binding profiles identified a conserved motif, mutations in which—including some found in human cancers—induced discrete changes in binding affinities in vitro and protein mislocalization in vivo. The data set reveals cooperativity as a key mechanism for membrane recruitment and, by enabling the interpretation of disease-associated mutations, suggests avenues for the design of small molecules targeting PH domains. Graphical abstract Teaser Vonkova et al. systematically quantify the lipid-binding properties of 91 pleckstrin homology (PH) domains using a physiological, quantitative, liposome microarray-based assay. The data set reveals that cooperativity between lipids is a key mechanism for membrane recruitment of PH domains.

Description: Publication date: Available online 20 August 2015 Source: Cell Reports Author(s): Ju-Hyun Lee, Mary Kate McBrayer, Devin M. Wolfe, Luke J. Haslett, Asok Kumar, Yutaka Sato, Pearl P.Y. Lie, Panaiyur Mohan, Erin E. Coffey, Uday Kompella, Claire H. Mitchell, Emyr Lloyd-Evans, Ralph A. Nixon Presenilin 1 (PS1) deletion or Alzheimer’s disease (AD)-linked mutations disrupt lysosomal acidification and proteolysis, which inhibits autophagy. Here, we establish that this phenotype stems from impaired glycosylation and instability of vATPase V0a1 subunit, causing deficient lysosomal vATPase assembly and function. We further demonstrate that elevated lysosomal pH in Presenilin 1 knockout (PS1KO) cells induces abnormal Ca 2+ efflux from lysosomes mediated by TRPML1 and elevates cytosolic Ca 2+ . In WT cells, blocking vATPase activity or knockdown of either PS1 or the V0a1 subunit of vATPase reproduces all of these abnormalities. Normalizing lysosomal pH in PS1KO cells using acidic nanoparticles restores normal lysosomal proteolysis, autophagy, and Ca 2+ homeostasis, but correcting lysosomal Ca 2+ deficits alone neither re-acidifies lysosomes nor reverses proteolytic and autophagic deficits. Our results indicate that vATPase deficiency in PS1 loss-of-function states causes lysosomal/autophagy deficits and contributes to abnormal cellular Ca 2+ homeostasis, thus linking two AD-related pathogenic processes through a common molecular mechanism. Graphical abstract Teaser Lee et al. present evidence establishing that Presenilin 1 loss of function elevates lysosomal pH via loss of V0a1 vATPase subunits. Besides disrupting autophagy, elevated lysosomal pH hyperactivates the TRPML1 calcium channel, causing increased lysosomal calcium efflux and cytosolic calcium elevation, thus linking two AD-related presenilin phenotypes to vATPase deficiency.

Description: Publication date: Available online 20 August 2015 Source: Cell Reports Author(s): Soraia Barão, Annette Gärtner, Eduardo Leyva-Díaz, Galina Demyanenko, Sebastian Munck, Tine Vanhoutvin, Lujia Zhou, Melitta Schachner, Guillermina López-Bendito, Patricia F. Maness, Bart De Strooper ΒACE1 is the major drug target for Alzheimer’s disease, but we know surprisingly little about its normal function in the CNS. Here, we show that this protease is critically involved in semaphorin 3A (Sema3A)-mediated axonal guidance processes in thalamic and hippocampal neurons. An active membrane-bound proteolytic CHL1 fragment is generated by BACE1 upon Sema3A binding. This fragment relays the Sema3A signal via ezrin-radixin-moesin (ERM) proteins to the neuronal cytoskeleton. APH1B-γ-secretase-mediated degradation of this fragment stops the Sema3A-induced collapse and sensitizes the growth cone for the next axonal guidance cue. Thus, we reveal a cycle of proteolytic activity underlying growth cone collapse and restoration used by axons to find their correct trajectory in the brain. Our data also suggest that BACE1 and γ-secretase inhibition have physiologically opposite effects in this process, supporting the idea that combination therapy might attenuate some of the side effects associated with these drugs. Graphical abstract Teaser Barão et al. show that the Alzheimer’s-disease-related proteases, BACE1 and APH1B-γ-secretase, control axonal guidance by regulating growth cone dynamics. BACE1 cleaves CHL1, inducing growth cone collapse. Subsequently, γ-secretase activity stops the collapse and axonal growth resumes. Therefore, testing of inhibitors of these proteases in humans should proceed with caution.

Description: Publication date: Available online 20 August 2015 Source: Cell Reports Author(s): Alexander Harms, Frédéric Valentin Stanger, Patrick Daniel Scheu, Imke Greet de Jong, Arnaud Goepfert, Timo Glatter, Kenn Gerdes, Tilman Schirmer, Christoph Dehio Toxin-antitoxin (TA) modules are ubiquitous molecular switches controlling bacterial growth via the release of toxins that inhibit cell proliferation. Most of these toxins interfere with protein translation, but a growing variety of other mechanisms hints at a diversity that is not yet fully appreciated. Here, we characterize a group of FIC domain proteins as toxins of the conserved and abundant FicTA family of TA modules, and we reveal that they act by suspending control of cellular DNA topology. We show that FicTs are enzymes that adenylylate DNA gyrase and topoisomerase IV, the essential bacterial type IIA topoisomerases, at their ATP-binding site. This modification inactivates both targets by blocking their ATPase activity, and, consequently, causes reversible growth arrest due to the knotting, catenation, and relaxation of cellular DNA. Our results give insight into the regulation of DNA topology and highlight the remarkable plasticity of FIC domain proteins. Graphical abstract Teaser Harms et al. reveal that the FicTA toxin-antitoxin module acts via adenylylation of DNA gyrase and topoisomerase IV. This modification inactivates both targets by blocking the ATPase activity that is central to their enzymatic functions, and it reversibly inhibits bacterial growth via the knotting, catenation, and relaxation of cellular DNA.

Description: Publication date: Available online 20 August 2015 Source: Cell Reports Author(s): Takaharu Kanno, Davide G. Berta, Camilla Sjögren The structural maintenance of chromosome (SMC) protein complexes cohesin and condensin and the Smc5/6 complex (Smc5/6) are crucial for chromosome dynamics and stability. All contain essential ATPase domains, and cohesin and condensin interact with chromosomes through topological entrapment of DNA. However, how Smc5/6 binds DNA and chromosomes has remained largely unknown. Here, we show that purified Smc5/6 binds DNA through a mechanism that requires ATP hydrolysis by the complex and circular DNA to be established. This also promotes topoisomerase 2-dependent catenation of plasmids, suggesting that Smc5/6 interconnects two DNA molecules using ATP-regulated topological entrapment of DNA, similar to cohesin. We also show that a complex containing an Smc6 mutant that is defective in ATP binding fails to interact with DNA and chromosomes and leads to cell death with concomitant accumulation of DNA damage when overexpressed. Taken together, these results indicate that Smc5/6 executes its cellular functions through ATP-regulated intermolecular DNA linking. Graphical abstract Teaser Kanno et al. have found that Smc5/6 interacts with DNA by two different mechanisms. One is based on electrostatic interactions that require ATP binding to Smc6. The other leads to topological entrapment and demands ATP hydrolysis by the complex. The results show that Smc5/6 is an ATP-dependent intermolecular DNA linker.

Description: Publication date: September 2015 Source: Journal of Hydrology: Regional Studies, Volume 4, Part B Author(s): J.S. Lessels, T.F.A. Bishop Study region South eastern Australia. Study focus This region is characterised with rainfall events that are associated with large exports of nutrients and sediments. Many water quality monitoring schemes use a form of event-based sampling to quantify these exports. Previous water quality studies that have evaluated different sampling schemes often rely on continuously monitored water quality data. However, many catchment authorities only have access to limited historical data which consists of event-based and monthly routine samples. Therefore there is a need to develop a method that assesses the importance of sampling events using information from limited historical data. This work presents a simulation based approach using unconditional simulation based on historical stream discharge. Such an approach offers site-specific information on optimal sampling schemes. A linear mixed model is used to model the relationship between total phosphorus and stream discharge and the auto-correlation of total phosphorus. New hydrological insights for the region The inclusion of event-based sampling improved annual load estimates of all sites with a maximum RMSE difference of 16.11 tonnes between event-based and routine sampling. Based on the accuracy of annual loads, event-based sampling was found to be more important in catchments with a large relief and high annual rainfall in this region. Using this approach, different sampling schemes can be compared based on limited historical data.

Description: The irrigation water available for agriculture will be scarce in the future due to increased competition for water with other sectors, and the issue may become more serious due to climate change. In Chile, the table grape is only cultivated under irrigation. A five-year research program (2007–2012) was carried out in the Aconcagua Valley, the central area of grapes in Chile, to evaluate the response of table grape vines (Vitis vinifera L., cv Thompson Seedless) to different volumes of irrigation water. Four irrigation treatments were applied: 60, 88, 120 and 157% of crop evapotranspiration (ETc) during the first four years, and 40, 54, 92 and 108% of ETc in the last year. Irrigation over 90%–100% of ETc did not increase fruit yield, whereas the application of water below 90% ETc decreased exportable yield, berry size and pruning weight. For example, 60% ETc applied water reduced exportable yield by 20%, and only 40% of the berries were in the extra and large category size, while pruning weight was 30% lower in comparison to the treatment receiving more water.

Description: Leaf length is a key factor in the economic value of different grass species and cultivars in forage production. It is also important for the survival of individual plants within a sward. The objective of this paper is to discuss the basis of within-species variation in leaf length. Selection for leaf length has been highly efficient, with moderate to high narrow sense heritability. Nevertheless, the genetic regulation of leaf length is complex because it involves many genes with small individual effects. This could explain the low stability of QTL found in different studies. Leaf length has a strong response to environmental conditions. However, when significant genotype × environment interactions have been identified, their effects have been smaller than the main effects. Recent modelling-based research suggests that many of the reported environmental effects on leaf length and genotype × environment interactions could be biased. Indeed, it has been shown that leaf length is an emergent property strongly affected by the architectural state of the plant during significant periods prior to leaf emergence. This approach could lead to improved understanding of the factors affecting leaf length, as well as better estimates of the main genetic effects.

Description: Publication date: 18 August 2015 Source: Cell Reports, Volume 12, Issue 7 Author(s): Marvin L. Meistrich, Gunapala Shetty In this issue of Cell Reports , DeFalco et al. (2015) characterize a novel macrophage population associated with the peritubular lamina of mouse testes. These macrophages may create a niche not for the self-renewal of stem cells but rather the induction of their differentiation.

Description: We develop isometry and inversion formulas for the Segal–Bargmann transform on odd-dimensional hyperbolic spaces that are as parallel as possible to the dual case of odd-dimensional spheres.

Description: Soil water potential (Ψ) controls the dynamics of water in soils and can therefore affect greenhouse gas fluxes. We examined the relationship between soil moisture content (θ) at five different levels of water potential (Ψ = 0, −0.05, −0.1, −0.33 and −15 bar) and greenhouse gas (carbon dioxide, CO2; nitrous oxide, N2O and methane, CH4) fluxes. The study was conducted in 2011 in a silt loam soil at Freeman farm of Lincoln University. Soil samples were collected at two depths: 0–10 and 10–20 cm and their bulk densities were measured. Samples were later saturated then brought into a pressure plate for measurements of Ψ and θ. Soil air samples for greenhouse gas flux analyses were collected using static and vented chambers, 30 cm in height and 20 cm in diameter. Determination of CO2, CH4 and N2O concentrations from soil air samples were done using a Shimadzu Gas Chromatograph (GC-14). Results showed that there were significant correlations between greenhouse gas fluxes and θ held at various Ψ in the 0–10 cm depth of soil group. For instance, θ at Ψ = 0 positively correlated with measured CO2 (p = 0.0043, r = 0.49), N2O (p = 0.0020, r = 0.64) and negatively correlated with CH4 (p = 0.0125, r = −0.44) fluxes. Regression analysis showed that 24%, 41% and 19% of changes in CO2, N2O and CH4 fluxes, respectively, were due to θ at Ψ = 0 (p 〈 0.05). This study stresses the need to monitor soil water potential when monitoring greenhouse gas fluxes.

Description: Publication date: Available online 19 August 2015 Source: Geoscience Frontiers Author(s): Noreen Mary Vielreicher, David Ian Groves, Neal Jesse McNaughton The Neoarchaean Kalgoorlie Gold Field contains the giant Golden Mile and world-class Mt Charlotte deposits, which have been the subject of much research for over 100 years. The Golden Mile deposit is a complex array of ductile to brittle vein and breccia lodes that are predominantly hosted in the highly-fractionated Golden Mile Dolerite sill. The Fimiston lodes comprise an array of narrow lodes that evolved broadly syn- to late-formation of the regional D2 NW-trending foliation. The lodes are characterized by pyrite veinlets and disseminations, quartz veinlets and breccias, and banded quartz-carbonate veins with sericite, carbonate, and pyrite-dominated alteration. Bonanza Green-Leader, or Oroya-style, lodes, with grades in excess of 1000g/t Au, are similar to the Fimiston-style lodes, but are characterized by abundant visible gold, native tellurium and more abundant telluride minerals within roscoelite-bearing alteration zones. The arguably structurally younger Mt Charlotte-style lodes are characterized by a pipe-shaped, coarse-grained quartz, carbonate and scheelite vein-stockwork with distinct vertically-zoned, carbonate-sericite-albite-pyrite±pyrrhotite dominant alteration assemblages around veins within Unit 8 of the Golden Mile Dolerite and porphyry dykes. The network of steep- and gently-dipping extension and shear fracture-fill veins are associated with NE-trending fault sets that cross cut the regional NW-trend. The deposit area is intruded by swarms of porphyry dykes, including syn-volcanic mafic dykes, early and volumetrically most significant c. 2.67 Ga feldspar-phyric porphyry dykes, as well as later c. 2.66‒2.65Ga calc-alkaline hornblende-phyric dykes associated with younger c. 2.65‒2.64Ga lamprophyre dykes. All post-volcanic dykes have similar orientations to the Fimiston lodes. The feldspar dykes are clearly overprinted by all styles of mineralization, although the relationship between hornblende-phyric and lamprophyre dykes and gold mineralization is more ambiguous. Most agree that gold mineralization was post-peak regional metamorphism of host rocks, although its relative structural timing is controversial. Direct timing constraints on gold mineralization indicate that Fimiston- and Mt Charlotte-style mineralization formed within a relative short period of time around 2.64Ga, and, as such, support a model of progressive deformation of a rheologically heterogeneous rock package late in the structural history. Fluid charactersitics, combined with the structural, metamorphic and absolute timing, support description of gold mineralization at the Golden Mile as orogenic and mesozonal, and this allows direct correlation with orogenic gold deposits worldwide, which classically formed during accretion along convergent margins throughout Earth history. Graphical abstract

Description: Estimates of bed roughness used for predictions of sediment transport are usually derived either from simple scalars of the physical roughness (i.e., ripple height or grain size) or from the hydrodynamic roughness length (Zo) based upon velocity gradient estimates in the benthic boundary layer. Neither parameter accounts for irregular bed features. This study re-evaluates the relation between hydrodynamic roughness and physical bed roughness using high-resolution seabed scanning in the inlet of a shallow lagoon. The statistically-robust relationship, based on a 1D statistical analysis of the seabed elevation at different locations of the Cabras lagoon. Sardinia, has been obtained between Zo and the topographical bed roughness Ks by defining Ks = 2*STD + skin friction, with STD the standard deviation of the seabed elevation variations. This correlation between Ks and Zo demonstrates that the roughness length is directly influenced by irregular bed features, and that the Reynolds number accounts for the total drag of the bed: the data points collapse on the Law of the Wall curves with a fitting factor x = 0.5. Further testing must be done in other locations and in the fully-rough domain in order to test how widely those new parameters can be applied.

Description: A device (prototype) with a working volume of 200 L was used to deplete olive mill wastewater (OMW) of polyphenols. The OMW transformed into feedstock by means of the device was then used for feeding a lab-scale photobioreactor, just for testing the production of bioH2. The main novelty of this prototype consists in the combination of several adsorbent matrices and the exploitation of their synergic action. In this investigation, three matrices have been used: active carbon, Azolla and zeolite. The device was operated at an olive oil company located in the heart of the Chianti zone (Province of Florence, Italy). The efficiency of polyphenol removal obtained using the device was ≥96%. The multi-matrix effluent (MMeff) generated was then used to obtain three different culture broths containing 25%, 50% and 100% of MMeff, respectively. The diluted (with water) culture broths were suitable for hydrogen generation, with the highest hydrogen production rate (12.7 mL H2/Lculture/h) being obtained using 50% MMeff. The hydrogen yields were: 334 mL H2/L of MMeff, when feeding the photofermenter with pure effluent (100%); 1308 mL H2/L of MMeff, with the half-diluted effluent (50%, v/v); and 432 mL H2/L of MMeff, with the highest-diluted effluent (25%, v/v).

Description: Using torus gauge fixing, Hahn in 2008 wrote down an expression for a Chern-Simons path integral to compute the Wilson Loop observable, using the Chern-Simons action \(S_{CS}^\kappa\), \(\kappa\) is some parameter. Instead of making sense of the path integral over the space of \(\mathfrak{g}\)-valued smooth 1-forms on \(S^2 \times S^1\), we use the Segal Bargmann transform to define the path integral over \(B_i\), the space of \(\mathfrak{g}\)-valued holomorphic functions over \(\mathbb{C}^2 \times \mathbb{C}^{i-1}\). This approach was first used by us in 2011. The main tool used is Abstract Wiener measure and applying analytic continuation to the Wiener integral. Using the above approach, we will show that the Chern-Simons path integral can be written as a linear functional defined on \(C(B_1^{\times^4} \times B_2^{\times^2}, \mathbb{C})\) and this linear functional is similar to the Chern-Simons linear functional defined by us in 2011, for the Chern-Simons path integral in the case of \(\mathbb{R}^3\). We will define the Wilson Loop observable using this linear functional and explicitly compute it, and the expression is dependent on the parameter \(\kappa\). The second half of the article concentrates on taking \(\kappa\) goes to infinity for the Wilson Loop observable, to obtain link invariants. As an application, we will compute the Wilson Loop observable in the case of \(SU(N)\) and \(SO(N)\). In these cases, the Wilson Loop observable reduces to a state model. We will show that the state models satisfy a Jones type skein relation in the case of \(SU(N)\) and a Conway type skein relation in the case of \(SO(N)\). By imposing quantization condition on the charge of the link \(L\), we will show that the state models are invariant under the Reidemeister Moves and hence the Wilson Loop observables indeed define a framed link invariant. This approach follows that used in an article written by us in 2012, for the case of \(\mathbb{R}^3\).

Description: The microbiological and chemical drinking water quality of 20 vulnerable Finnish small groundwater supplies was studied in relation to environmental risk factors associated with potential sources of contamination. The microbiological parameters analyzed included the following enteric pathogens: Giardia and Cryptosporidium, Campylobacter species, noroviruses, as well as indicator microbes (Escherichia coli, intestinal enterococci, coliform bacteria, Clostridium perfringens, Aeromonas spp. and heterotrophic bacteria). Chemical analyses included the determination of pH, conductivity, TOC, color, turbidity, and phosphorus, nitrate and nitrite nitrogen, iron, and manganese concentrations. Giardia intestinalis was detected from four of the water supplies, all of which had wastewater treatment activities in the neighborhood. Mesophilic Aeromonas salmonicida, coliform bacteria and E. coli were also detected. None of the samples were positive for both coliforms and Giardia. Low pH and high iron and manganese concentrations in some samples compromised the water quality. Giardia intestinalis was isolated for the first time in Finland in groundwater wells of public water works. In Europe, small water supplies are of great importance since they serve a significant sector of the population. In our study, the presence of fecal indicator bacteria, Aeromonas and Giardia revealed surface water access to the wells and health risks associated with small water supplies.

Description: Publication date: Available online 24 August 2015 Source: Journal of Asian Ceramic Societies Author(s): Dmytro Demirskyi, Yoshio Sakka, Oleg Vasylkiv The in situ synthesis/consolidation of B 4 C-TaB 2 eutectic composites by spark plasma sintering (SPS) is reported. The microstructure–property relations were determined for composites with the B 4 C-TaB 2 eutectic composition as functions of TaB 2 content, and TaB 2 -TaB 2 interlamellar spacing. A clear maximum in fracture toughness was identified (∼4.5 MPa m 1/2 ) for eutectic composites with interlamellar spacing between 0.9 and 1.1 μm. The composites with the hypereutectic composition of 40 mol.% TaB 2 obtained by SPS exhibited lower Vickers hardness (25–26 GPa) but higher indentation fracture toughness (up to 4.9 MPa m 1/2 ) than eutectic composites with 30–35 mol.% of TaB 2 .

Description: Publication date: September 2015 Source: Journal of Hydrology: Regional Studies, Volume 4, Part B Author(s): Hayet Chihi, Ghislain de Marsily, Habib Belayouni, Houcine Yahyaoui Study region “Jeffara de Medenine” aquifer system in south-eastern Tunisia. Study focus This study investigates the role of fault structures in the distribution of hydrogeochemical facies and groundwater compartmentalization for the aquifer system. New hydrological insights for the region The proposed methodology, including seismic structural study, hierarchical cluster analysis and geostatistical methods, allowed an efficient multi-element characterization of the spatial patterns of the structural elements in the aquifers and of the hydrogeological parameters used in a spatial cross-correlation to explore the dependence of the geochemical properties in each “geochemical population” on the hosting structural compartment to delineate the different geochemical compartments. The tectonic studies showed that the lateral extent of the aquifers is controlled by normal faults. The multivariate statistical analysis revealed a strong spatial coherence between hydrogeochemical facies clustering and the reservoir compartments at both large and small scales. The kriged maps of major-ion concentrations and of total dissolved solids in the aquifers were then analyzed and compared with the reservoir facies distribution for each compartment, the geometric characteristics of the aquifer, and the piezometric level trends. This allowed to characterize the hydraulic behavior of the Medenine fault and to understand the underlying physical and chemical processes having led to the spatial distribution of the geochemical properties, and thus, the hydrogeochemical functioning of the aquifers.

Description: Publication date: September 2015 Source: Journal of Hydrology: Regional Studies, Volume 4, Part B Author(s): Marnie L. Atkins, Isaac R. Santos, Damien T. Maher Study region This study investigates dissolved methane distribution in groundwater from the Richmond River Catchment (New South Wales, Australia) before proposed coal seam gas (CSG, or coal bed methane) development. Study focus Unconventional gas exploration has rapidly expanded in recent years. However, the impact of these operations on groundwater systems is poorly understood. A total of 91 groundwater samples were analyzed from 6 geological units. Our observations act as regional baseline research prior to CSG extraction and may assist with long term impact assessment. New hydrological insights for the region Methane was found in all geological units ranging between 0.26 and 4427 μg L −1 (median 10.68 μg L −1 ). Median methane concentrations were highest in chloride-type groundwater (13.26 μg L −1 , n = 58) while bicarbonate-type groundwater had lower concentrations (3.71 μg L −1 ). Groundwater from alluvial sediments had significantly higher median methane concentrations (91.46 μg L −1 ) than groundwater from both the basalt aquifers (0.7 μg L −1 ) and bedrock aquifers (4.63 μg L −1 ); indicating geology was a major driver of methane distribution. Methane carbon stable isotope ratios ranged from –90.9‰ to –29.5‰, suggesting a biogenic origin with some methane oxidation. No significant correlations were observed between methane concentrations and redox indicators (nitrate, manganese, iron and sulphate) except between iron and methane in the Lismore Basalt ( r 2 = 0.66, p 〈 0.001), implying redox conditions were not the main predictor of methane distribution.

Description: For 20 years, the number of resource policy approaches with direct and indirect relations to raw materials, resource and material efficiency has grown enormously at national and international level. This discussion paper makes an inventory of different political and regulatory approaches that contain a direct or indirect reference to resources such as construction materials, industrial minerals, or metals. They are examined and evaluated regarding foci and resource priorities as well as further categories such as target lines, governance levels, indicators used, integration into wider target systems, specification, and implementation. The aim is to provide an overview of the spectrum of resource objectives in international, European, and national strategies, programs, and initiatives. The closer analysis of raw material targets embedded in the policy programs and legal approaches reveals that most goals lack a time frame and a concrete vision, thus remain at a strategic level. To complement the overview, the state of research in the field of modeling and simulation is briefly discussed. Concluding remarks concerning their relation to the objectives identified and the task of target setting complete the discussion.

Description: The identification of an appropriate justification for Agri-Environment-Climate (AEC) payments is a crucial issue in the new Rural Development Programme (RDP). Given the environmental importance of grasslands in Trentino (Italy), the Management Authority in charge of the RDP decided to integrate an approach based on Ecosystem Services (ES) into the calculation of AEC payments. The paper presents the methodology used for this approach as well as the preliminary results. The first step entails building a probabilistic model for the ES, named Sustainable Fodder Production. Model outputs are then integrated with the accounting results based on the Farm Accountancy Data Network (FADN) database (2009–2012) with the aim of calculating the additional costs and income waived due to the environmental commitments deriving from the sustainable management of permanent grassland in livestock farming. Sustainability measures imply more extensive management practices that maintain meadows in a healthy state.

Description: Bubbles generated by breaking waves can drive significant gas exchange between the ocean and atmosphere, but the role of bubble-mediated gas transfer in estuaries is unknown. Here, backscatter data from 41 acoustic Doppler current profiler stations was analyzed to assess subsurface bubble distributions in nine estuaries along the U.S. East and Gulf Coast. Wind speed, wind direction, and current velocity were the dominant controls on bubble entrainment, but the relative importance of these physical drivers depended on local geomorphology. Bubble entrainment in high-current or shallow, long-fetch estuaries began at wind speeds 〈5 m s−1. In deep or fetch-limited estuaries, bubble entrainment was less frequent and generally began at higher wind speeds. Data observed during several storms suggests that episodic bubble-driven gas exchange may be an important component of annual CO2 fluxes in large, shallow estuaries but would be less significant in other coastal systems.

Description: Publication date: Available online 13 August 2015 Source: Cell Reports Author(s): Kavita R. Sharma, Brittany L. Enzmann, Yvonne Schmidt, Dani Moore, Graeme R. Jones, Jane Parker, Shelley L. Berger, Danny Reinberg, Laurence J. Zwiebel, Bernhard Breit, Jürgen Liebig, Anandasankar Ray The sophisticated organization of eusocial insect societies is largely based on the regulation of complex behaviors by hydrocarbon pheromones present on the cuticle. We used electrophysiology to investigate the detection of cuticular hydrocarbons (CHCs) by female-specific olfactory sensilla basiconica on the antenna of Camponotus floridanus ants through the utilization of one of the largest family of odorant receptors characterized so far in insects. These sensilla, each of which contains multiple olfactory receptor neurons, are differentially sensitive to CHCs and allow them to be classified into three broad groups that collectively detect every hydrocarbon tested, including queen and worker-enriched CHCs. This broad-spectrum sensitivity is conserved in a related species, Camponotus laevigatus , allowing these ants to detect CHCs from both nestmates and non-nestmates. Behavioral assays demonstrate that these ants are excellent at discriminating CHCs detected by the antenna, including enantiomers of a candidate queen pheromone that regulates the reproductive division of labor. Graphical abstract Teaser Sharma et al. show that ants can detect a number of hydrocarbons present on the cuticle, therefore recognizing different castes such as workers and queens from their own colony as well as different colonies. They also show that ants are able to smell and discriminate minor differences among hydrocarbons.

Description: Publication date: Available online 13 August 2015 Source: Cell Reports Author(s): Sanjeev Kumar, Jing Liu, Paul Pang, A. Michaela Krautzberger, Antoine Reginensi, Haruhiko Akiyama, Andreas Schedl, Benjamin D. Humphreys, Andrew P. McMahon After acute kidney injury (AKI), surviving cells within the nephron proliferate and repair. We identify Sox9 as an acute epithelial stress response in renal regeneration. Translational profiling after AKI revealed a rapid upregulation of Sox9 within proximal tubule (PT) cells, the nephron cell type most vulnerable to AKI. Descendants of Sox9 + cells generate the bulk of the nephron during development and regenerate functional PT epithelium after AKI-induced reactivation of Sox9 after renal injury. After restoration of renal function post-AKI, persistent Sox9 expression highlights regions of unresolved damage within injured nephrons. Inactivation of Sox9 in PT cells pre-injury indicates that Sox9 is required for the normal course of post-AKI recovery. These findings link Sox9 to cell intrinsic mechanisms regulating development and repair of the mammalian nephron. Graphical abstract Teaser Surviving tubular epithelial cells repair the nephron after acute kidney injury (AKI). Kumar et al. identify Sox9 activation as a rapid response to AKI within repairing cells of the damaged proximal tubule segment. Sox9 activation is required for a normal repair process.

Description: Publication date: Available online 13 August 2015 Source: Cell Reports Author(s): Rheinallt M. Jones, Chirayu Desai, Trevor M. Darby, Liping Luo, Alexandra A. Wolfarth, Christopher D. Scharer, Courtney S. Ardita, April R. Reedy, Erin S. Keebaugh, Andrew S. Neish An optimal gut microbiota influences many beneficial processes in the metazoan host. However, the molecular mechanisms that mediate and function in symbiont-induced host responses have not yet been fully characterized. Here, we report that cellular ROS enzymatically generated in response to contact with lactobacilli in both mice and Drosophila has salutary effects against exogenous insults to the intestinal epithelium via the activation of Nrf2 responsive cytoprotective genes. These data show that the xenobiotic-inducible Nrf2 pathway participates as a signaling conduit between the prokaryotic symbiont and the eukaryotic host. Indeed, our data imply that the capacity of lactobacilli to induce redox signaling in epithelial cells is a highly conserved hormetic adaptation to impel cellular conditioning to exogenous biotic stimuli. These data also highlight the role the microbiota plays in eukaryotic cytoprotective pathways and may have significant implications in the characterization of a eubiotic microbiota. Graphical abstract Teaser Jones et al. report that the commensal gut bacterial taxa lactobacilli are able to mediate beneficial cytoprotective effects in the gut of both flies and mice. These highly conserved events are mediated by ROS-dependent activation of the Nrf2 xenobiotic pathway and conserved effector genes.

Description: Publication date: Available online 13 August 2015 Source: Cell Reports Author(s): Neel Mehta, Arthur H. Cheng, Cheng-Kang Chiang, Lucia Mendoza-Viveros, Harrod H. Ling, Abhilasha Patel, Bo Xu, Daniel Figeys, Hai-Ying M. Cheng The pacemaker properties of the suprachiasmatic nucleus (SCN) circadian clock are shaped by mechanisms that influence the expression and behavior of clock proteins. Here, we reveal that G-protein-coupled receptor kinase 2 (GRK2) modulates the period, amplitude, and entrainment characteristics of the SCN. Grk2 -deficient mice show phase-dependent alterations in light-induced entrainment, slower recovery from jetlag, and longer behavioral rhythms. Grk2 ablation perturbs intrinsic rhythmic properties of the SCN, increasing amplitude and decreasing period. At the cellular level, GRK2 suppresses the transcription of the mPeriod1 gene and the trafficking of PERIOD1 and PERIOD2 proteins to the nucleus. Moreover, GRK2 can physically interact with PERIOD1/2 and promote PERIOD2 phosphorylation at Ser545, effects that may underlie its ability to regulate PERIOD1/2 trafficking. Together, our findings identify GRK2 as an important modulator of circadian clock speed, amplitude, and entrainment by controlling PERIOD at the transcriptional and post-translational levels. Graphical abstract Teaser Mehta et al. demonstrate the importance of GRK2 in regulating both the pace of the circadian clock and its response to environmental time cues (i.e., light). GRK2 functionally interacts with the molecular clock at the transcriptional and post-translational levels, dampening mPeriod1 gene transcription and suppressing nuclear trafficking of PERIOD1/2 proteins.

Description: Publication date: Available online 13 August 2015 Source: Cell Reports Author(s): Juan F. Linares, Angeles Duran, Miguel Reina-Campos, Pedro Aza-Blanc, Alex Campos, Jorge Moscat, Maria T. Diaz-Meco The mTORC1 complex is central to the cellular response to changes in nutrient availability. The signaling adaptor p62 contributes to mTORC1 activation in response to amino acids and interacts with TRAF6, which is required for the translocation of mTORC1 to the lysosome and the subsequent K63 polyubiquitination and activation of mTOR. However, the signal initiating these p62-driven processes was previously unknown. Here, we show that p62 is phosphorylated via a cascade that includes MEK3/6 and p38δ and is driven by the PB1-containing kinase MEKK3. This phosphorylation results in the recruitment of TRAF6 to p62, the ubiquitination and activation of mTOR, and the regulation of autophagy and cell proliferation. Genetic inactivation of MEKK3 or p38δ mimics that of p62 in that it leads to inhibited growth of PTEN-deficient prostate organoids. Analysis of human prostate cancer samples showed upregulation of these three components of the pathway, which correlated with enhanced mTORC1 activation. Graphical abstract Teaser Linares et al. identify a kinase cascade that regulates the phosphorylation of the signal adaptor p62 in response to amino acids to control mTORC1 activation. This nutrient-sensing mechanism is relevant for autophagy regulation and tumor growth.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 28 Author(s): Yui Masuki This study clarifies the role of an NGO in transforming the discriminatory social status of the Dalits (Untouchables), and its implications for sustainable community development. Subduing the discriminatory relationship between Dalits and other local people will not only uplift them socioeconomically, but also expand their opportunity for communication, enabling the construction of inter-personal relationships between the discriminating and the discriminated against. The NGO plays an important role in facilitating this communication and implementing national and global level sanitation. This study illustrates the dynamic process of transforming discriminatory social relationships and the complicated mechanism of Dalit discrimination in contemporary India.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 28 Author(s): Mohammad Razif, Soemarno, Bagyo Yanuwiadi, Arief Rachmansyah, Prawira Fajarindra Belgiawan This study aims to implement the linear regression method to generate a linear regression equation that can be used in the study of EIA (Environmental Impact Assessment) to predict the cost of WWTP (Wastewater Treatment Plant) of the data wastewater flowrate with case study of ten malls in Surabaya city.Some previous researchers have produced data on a linear regression WWTP construction costs and wastewater flowrate.In the EIA study in Indonesia, management and monitoring costs include costs of construction, operation and maintenance costs, and monitoring costs.Therefore, these costs will be calculated for WWTP of malls with unit prices prevailing in Surabaya city. The methodology of the study consists of the calculation of the construction cost, the calculation of operation and maintenance cost, the calculation of monitoring cost, and the process of making a linear regression curve. The result showed that from the ten malls, the smallest flowrate was 0.88 l/sec and 6.72 l/sec the largest, while the calculation of the cost was gained at the least as 577x10 6 IDR and the biggest cost was 2163 x10 6 IDR. The linear regression curve between the flow rate WWTP (as independentvariables in the X axis) and the total cost WWTP (as dependent variables in the Y-axis) has produced a regression equation of Y = 358 + 271X. The result of the linear regression equation can be used directly by the EIA committee in Surabaya city and activityinitiator of mall to predict the cost of WWTP quickly and to decide the environmental feasibility of the mall.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 28 Author(s): Obie Farobie, Yukihiko Matsumura Biodiesel was produced via non-catalytic transesterification in supercritical methanol using a novel spiral reactor. This spiral reactor could serve as a heat exchanger, thus it provided the advantage of being able to recover the heat. Transesterification was carried out at 270-400°C, a pressure of 20 MPa, oil-to-methanol molar ratio of 1:40, and reaction time of 3-30min. Using this technique, a complete conversion of fatty acid methyl ester (FAME) (100 wt%) was obtained in a short reaction time of 10 min at 350°C and oil-to-methanol molar ratio of 1:40 under a reactor pressure of 20 MPa. The result revealed that biodiesel yield conducted in spiral reactor is higher than that in batch reactor at the same reaction conditions. The kinetic model of canola oil conversion to biodiesel in supercritical methanol was also determined.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 28 Author(s): Supriyono, Hedi Surahman, Yuni Krisyuningsih Krisnandi, Jarnuzi Gunlazuardi Relationship between loading level and some physical properties of the SnO 2 -F film has been investigated. SnO 2 -F thin films were prepared by spray pyrolysis technique using modified respiratory therapist nebulizer at substrate temperature in the range of 400-520̊C. A stannous chloride solution was used as precursor and ammonium fluoride (NH 4 F) as dopant with 20% [F]/[Sn] ratio. The sheet resistance was found to decrease withthe increasing loading level, and sheet resistancebecame steady after the loading level was greater than0.45 mg/cm 2 . The transmittance was found to decrease with the increase of loading level but the decrease was not significant. The transparent conductive oxide of SnO 2 -F thin films were obtained, with transmittance was greater than 82.5% and sheet resistance of 21.4 Ω/sq. This results are comparable with the reported values (81.9% and 21.8 Ω/sq) [1] and this film can be used for efficient support of dye-sensitized solar cells electrode.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 28 Author(s): Aziza Hamed Al-Farsi, Hameed Sulaiman, Hassan Ali Al-Reasi Protection of the ecosystems ultimately contributes towards their sustainability. Presence of high levels of toxic metals in seafood is considered as an environmental warning for possible influences on the ecosystem components and public health. This research quantified the concentrations of cadmium (Cd), chromium (Cr), copper (Cu), manganese (Mn), lead (Pb), vanadium (V), and zinc (Zn) in sediments and edible soft tissue of shellfish Strombus ( Conomurex ) persicus from the Sea of Oman. In both matrices, metals exhibited no general trend in the distribution of metals between the sampling sites. In shellfish, metal levels were below the international maximum permissible guidelines, illustrating safe consumption of this seafood item. The calculated risk factor ( E r i ) for Cr, Cu, Pb, and Zn revealed no ecological damage should be anticipated at the reported metal concentrations in the sediment samples. However, Cd had higher E r i range, suggesting moderate to considerable ecological risk. For transfer of metals from sediment to S . persicus , bioaccumulation factors (BAF) estimated for Cd, Cu, and Zn were above 1.0, indicating tendency of these metals to accumulate in S . persicus . While the BAF and E r i values for Zn were insignificantly correlated, BAF values for Cd and Cu were inversely related to their corresponding E r i values. Nevertheless, correlation was not statistically significant for Cu. For Cd, it seems that sediments having higher E r i may not be necessarily resulting in higher tissue Cd burdens of S . persicus . In conclusion, it appears that the use of correlation between BAF and E r i to examine transfer of metals from abiotic component to organisms in natural waters is limited.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 28 Author(s): Tito Sumandono, Henderson Saragih, Migirin, Takashi Watanabe, Rudianto Amirta In this research, decolorization of Remazol Brilliant Blue R (RBBR) was investigated using a new isolated white rot fungus, strain KRUS-G, collected from Mulwarman University Botanical Garden,Samarinda, East Kalimantan, Indonesia. The results showed that strain KRUS-G decolorized RBBR efectively compared to Phanerochaetecrysosporium and Ceriporiopsissubvermispora . The highest decolorization was obtained at pH 4 with 89% loss of RBBR used. The effective decolorization was also observed in the high concentration of RBBR (1500 ppm).Concentration of RBBR at 500, 1000, and 1500 ppm caused slightly decreasing pattern on mycelia growth. Rapid decolorization was occurred within 4 days of incubation. We suggest that laccase played an important role on the decolorizationeven high activity ofMnP was also detected.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 28 Author(s): Arry Y. Nurhayati, Yuda C. Hariadi, P. Lestari Demands on an early detection method for lead (Pb), found as a pollutant in soil, are urgently needed to improve awareness on the continuing usage of waste water and industrial effluent for the agricultural practices especially in East Java, Indonesia. Marsilea crenata plant grown in a glasshouse under various concentration of Pb to indicate its tolerance to Pb stress is measured by its weekly biolectrical responses. The result shows that increasing Pb concentration tends to inhibit the plant's growth. It is concluded that the method is viable as an early detection remediation process to protect agricultural economy and human security.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 28 Author(s): Santosa S. Putra, Mick v.d. Wegen, J. Reyns, Arthur v. Dam, D.P. Solomatine, J.A. Roelvink A model calibration based on the distributed multi stations approach is necessary towards model implementation in the operational phase. In this study, a tree dimensional (3D) hydrodynamic and salinity dynamic model of an estuary was simulated using DFlow Flexible Mesh program, which is developed by Deltares. Specifically, this research was focused on the Columbia Estuary case study, which is situated in Oregon, United States. The preconfigured model was calibrated based on 15 measurement stations that are spread along the estuary. Furthermore, a detail portion data with an average interval of 1 minute were used during the calibration process. The model performances were improved by considering the data denial concept. The data denial concept was introduced by neglecting inconsistence data across its temporal and spatial variability. In this particular case, it was revealed that the downstream data, which have high salinity value, tends to produce high contribution to the root mean square error of the model result. In conclusion, the upstream data have immense variable fluctuation rate and therefore it is more sensitive to give lower coefficient of determination. Therefore, there must be a trade of between good estuary model performance and upstream station data reliability.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 28 Author(s): Hoang Trung Thanh, Helmut Yabar, Yoshiro Higano Hanoi, the capital of Vietnam, is home to approximately 7 million people. In 2011, the city generated about 2,372,500 tons of municipal solid waste (MSW) (accounting for 11% of national generation) and the collection rate reached 85%, of which 84% was sent directly to landfills (without landfill gas capture systems). This conventional practice has caused not only adverse environmental impacts but also increased greenhouse gas (GHG) emissions and loss of recyclable resources. Since most of the waste generated in the city is organic waste (it accounts for 71% of municipal solid waste), there is high potential for organic waste recovery of MSW in Hanoi. This paper analyzes the potential for environmental benefits of introducing composting of municipal organic waste by proposing five alternative scenarios that range from current situation to composting of both commercial and household organic waste. In order to evaluate the environmental performance of the scenarios, we used three indicators: organic fertilizer production, landfill life extension, and GHG emission reduction. The results show that composting could produce a huge amount of organic fertilizer (i.e. from 6,424to 218,650 tons/year) depending on the scenarios. Diversion of organic waste to composting could reduce the amount of waste disposed in landfills resulting in extending landfill life significantly. Therefore, landfill lifecould be extended from 0.5 to 8.7 years compared to the current situation. Current MSW management practices contributed the highest amount of GHG emission accounting for 1,322,928 tonsCO 2 -eq/year, whereas the proposed scenarios decrease emissions in accordance with increasing the amount of organic waste used for composting. The estimated emission reduction from the proposed scenarios ranges from 15% to 98% compared to the current situation. The results suggest that composting could bring significant environmental benefits and is a key solution toward sustainable solid waste management for Hanoi city. In addition, composting highlights the potential of climate protection in the waste management sector.

Description: Publication date: 2015 Source: Procedia Environmental Sciences, Volume 28 Author(s): Yusuf Wibisono, Slamet Widodo The use of crude vegetable oils without degumming, during biodiesel production, might decrease the conversion rate and be difficult to separate glycerol from biodiesel. Ultrafiltration is promising technology for gum removal from crude vegetable oils. However, since the oil constituents have very close molecular weight, degumming process by membrane is relatively difficult. In order to understand the membrane degumming process of corn-oil, a prediction of concentration boundary layer thickness was calculated using a CFD model. An artificial neural network is developed to learn the relationship between Reynolds and Schmidt numbers of feed solution which affects the boundary layer thickness along the membrane tube.

Description: Publication date: Available online 29 July 2015 Source: Geodesy and Geodynamics Author(s): Zhou Rui, Wu Xiaoping How to deal with colored noises of GOCE (Gravity field and steady – state Ocean Circulation Explorer) satellite has been the key to data processing. This paper focused on colored noises of GOCE gradient data and the frequency spectrum analysis. According to the analysis results, gravity field model of the optimal degrees 90–240 is given, which is recovered by GOCE gradient data. This paper presents an iterative Wiener filtering method based on the gravity gradient invariants. By this method a degree-220 model was calculated from GOCE SGG (Satellite Gravity Gradient) data. The degrees above 90 of ITG2010 were taken as the prior gravity field model, replacing the low degree gravity field model calculated by GOCE orbital data. GOCE gradient colored noises was processed by Wiener filtering. Finally by Wiener filtering iterative calculation, the gravity field model was restored by space-wise harmonic analysis method. The results show that the model's accuracy matched well with the ESA's (European Space Agency) results by using the same data.

Description: Data on fresh fruit and vegetable shrink in supermarkets is important to help understand where and how much shrink could potentially be reduced by supermarkets to increase their profitability. This study provides: (1) shrink estimates for 24 fresh fruits and 31 fresh vegetables in U.S. supermarkets in 2011 and 2012; and (2) retail-level food loss. For each covered commodity, supplier shipment data was aggregated from a sample of 2900 stores from one national and four regional supermarket retailers in the United States, and this sum was then compared with aggregated point-of-sale data from the same stores to estimate the amount of shrink by weight and shrink rates. The 2011–2012 average annual shrink rates for individual fresh vegetables varied from 2.2 percent for sweet corn to 62.9 percent for turnip greens and for individual fresh fruit ranged from 4.1 percent for bananas to 43.1 percent for papayas. When these shrink estimates were used in the Loss-Adjusted Food Availability data series, annual food loss for these commodities totaled 5.9 billion pounds of fresh fruit and 6.1 billion pounds of fresh vegetables. This study extends the literature by providing important information on where and how much shrink could potentially be reduced. Precise comparisons across studies are difficult. This information, combined with information on available and cost-effective technologies and practices, may help supermarkets target food loss reduction efforts though food loss will never be zero.

Description: Heat flow of the sedimentary succession of the Eastern Canada Sedimentary Basins varies from 40 mW/m2 close to the exposed shield in the north to high 60–70 mW/m2 in the southwest–northeast St. Lawrence corridor. As high fluid flow rates are required for a successful geothermal application, the most important targets are deep existing permeable aquifers rather than hard rock, which would need to be fracked. Unfortunately, the ten most populated Québec urban centers are in the areas where the Grenville (Canadian Shield) is exposed or at shallow depths with sedimentary cover where temperatures are 30 °C or less. The city of Drummondville will be the exception, as the basement deepens sharply southwest, and higher temperatures reaching >120 °C are expected in the deep Cambrian sedimentary aquifers near a 4–5-km depth. Deep under the area where such sediments could be occurring under Appalachian nappes, temperatures significantly higher than 140 °C are predicted. In parts of the deep basin, temperatures as high as 80 °C–120 °C exist at depths of 3–4 km, mainly southeast of the major geological boundary: the Logan line. There is a large amount of heat resource at such depths to be considered in this area for district heating.

Description: This research looks at coupling desalination with renewable energy sources to create a high-value product (treated water) from two low value resources (brackish groundwater and intermittent solar energy). Desalination of brackish groundwater is already being considered as a potential new water supply in Texas. This research uses Texas as a testbed for spatially-resolved analysis techniques while considering depth to brackish groundwater, water quality, and solar radiation across Texas to determine the locations with the best potential for integrating solar energy with brackish groundwater desalination. The framework presented herein can be useful for policymakers, regional planners, and project developers as they consider where to site desalination facilities coupled with solar photovoltaics. Results suggest that the northwestern region of Texas—with abundant sunshine and groundwater at relatively shallow depths and low salinity in areas with freshwater scarcity—has the highest potential for solar powered desalination. The range in capacity for solar photovoltaic powered reverse osmosis desalination was found to be 1.56 × 10—6 to 2.93 × 10—5 cubic meters of water per second per square meter of solar panel (m3/s/m2).

Description: Ground source heat pump (GSHP) systems have been proven to have higher efficiency compared to conventional air source heat pump systems for space heating and cooling applications. While vertical ground heat exchangers (GHE) are favorable in GSHP installation, this type of configuration requires higher capital costs as opposed to horizontal configuration. Numerical simulation has been used to accurately predict the thermal performance of GHE. In this paper, numerical analysis of thermal performance for slinky horizontal GHE loops in different orientations and operation modes is discussed. It was found that the loop orientation is not so important due to the little effect it has on thermal performance. While the mean heat exchange rate of copper loop increases 48% compared to HDPE loop, the analysis supports the common claim that heat exchange rate is predominantly limited by the thermal conductivity of the ground. With the same amount of circulation work, the mean heat exchange rate increases by 83%–162% when operated in parallel loops operations. The performance in these operations can be further optimized to 10%–14% increase when spacing between adjacent loops was provided. The spacing helps to minimize interference of heat flow that would penalize the overall thermal performance.

Description: Small Island Developing States (SIDS) of the Pacific over the last decade have established some of the most ambitious renewable energy targets in the world. The promotion of renewable energy has been motivated by a desire to lessen dependence on fossil fuels, given the adverse economic impacts of high oil prices on these countries. Efforts to attract development assistance and to strengthen the position of Pacific SIDS in climate change negotiations have likely also played a role. This paper explores the development of renewable energy resources in the Pacific through a public policy lens. The ambitious renewable energy targets established by Pacific SIDS are argued to be appropriate in some cases, but in other cases are criticised on economic grounds. A potential trade-off is identified between the risk mitigation benefits and poverty alleviation benefits of different renewable technology investments, with questions raised about whether support for the former rather than the latter by development partners is appropriate. A number of institutional and financial challenges to the development of renewable energy resources in Pacific SIDS are also discussed.

Description: Publication date: 4 August 2015 Source: Cell Reports, Volume 12, Issue 5 Author(s): Obinna Chijioke, Anne Müller, Regina Feederle, Mario Henrique M. Barros, Carsten Krieg, Vanessa Emmel, Emanuela Marcenaro, Carol S. Leung, Olga Antsiferova, Vanessa Landtwing, Walter Bossart, Alessandro Moretta, Rocio Hassan, Onur Boyman, Gerald Niedobitek, Henri-Jacques Delecluse, Riccarda Capaul, Christian Münz

Description: Publication date: Available online 6 August 2015 Source: Cell Reports Author(s): Matthew T. Maurano, Hao Wang, Sam John, Anthony Shafer, Theresa Canfield, Kristen Lee, John A. Stamatoyannopoulos Although DNA methylation is commonly invoked as a mechanism for transcriptional repression, the extent to which it actively silences transcription factor (TF) occupancy sites in vivo is unknown. To study the role of DNA methylation in the active modulation of TF binding, we quantified the effect of DNA methylation depletion on the genomic occupancy patterns of CTCF, an abundant TF with known methylation sensitivity that is capable of autonomous binding to its target sites in chromatin. Here, we show that the vast majority (>98.5%) of the tens of thousands of unoccupied, methylated CTCF recognition sequences remain unbound upon abrogation of DNA methylation. The small fraction of sites that show methylation-dependent binding in vivo are in turn characterized by highly variable CTCF occupancy across cell types. Our results suggest that DNA methylation is not a primary groundskeeper of genomic TF landscapes, but rather a specialized mechanism for stabilizing intrinsically labile sites. Graphical abstract Teaser Alterations of DNA methylation in malignancy and development are frequently interpreted as affecting transcriptional activity. Maurano et al. find that, upon genomic abrogation of DNA methylation, binding of the canonically methylation-sensitive transcriptional regulator CTCF is largely unaffected. Their results suggest that a limited set of methylation-sensitive CTCF sites are variable across cell types and that key sequence and chromatin features predict methylation sensitivity of CTCF binding.

Description: Publication date: Available online 6 August 2015 Source: Cell Reports Author(s): Swea-Ling Khaw, Chua Min-Wen, Cheng-Gee Koh, Bing Lim, Ng Shyh-Chang Oocyte factors not only drive somatic cell nuclear transfer reprogramming but also augment the efficiency and quality of induced pluripotent stem cell (iPSC) reprogramming. Here, we show that the oocyte-enriched factors Tcl1 and Tcl1b1 significantly enhance reprogramming efficiency. Clonal analysis of pluripotency biomarkers further show that the Tcl1 oocyte factors improve the quality of reprogramming. Mechanistically, we find that the enhancement effect of Tcl1b1 depends on Akt, one of its putative targets. In contrast, Tcl1 suppresses the mitochondrial polynucleotide phosphorylase (PnPase) to promote reprogramming. Knockdown of PnPase rescues the inhibitory effect from Tcl1 knockdown during reprogramming, whereas PnPase overexpression abrogates the enhancement from Tcl1 overexpression. We further demonstrate that Tcl1 suppresses PnPase’s mitochondrial localization to inhibit mitochondrial biogenesis and oxidation phosphorylation, thus remodeling the metabolome. Hence, we identified the Tcl1-PnPase pathway as a critical mitochondrial switch during reprogramming. Graphical abstract Teaser Khaw et al. identify the oocyte Tcl1-PnPase pathway as a critical mitochondrial switch during reprogramming into iPSCs. They find that Tcl1 suppresses the mitochondrial localization of PNPase, thus inhibiting mitochondrial biogenesis and oxidation phosphorylation.