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  • Cell & Developmental Biology  (1,384)
  • 1990-1994  (1,384)
  • 1992  (1,384)
  • 1
    Electronic Resource
    Electronic Resource
    1,25-dihydroxy-vitamin-D3 enhances antiproliferative effect and transcription of TGF-β1 on human keratinocytes in culture (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 151 (1992), S. 579-587 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Both TGF-β and 1,25-dihydroxy-vitamin-D3 (1,25(OH)2D3) have been reported to decrease the proliferation of normal human keratinocytes. The effect and expression of TGF-β in keratinocytes treated with 1,25(OH)2D3 was investigated. Human keratinocytes were grown in the presence of various concentrations of TGF-β and/or 1,25(OH)2D3 prior to enumeration. TGF-β, alone, has a half maximal dose of inhibition (ED50) of approximately 750 pg/ml after seven days in culture in Keratinocyte Growth Medium (KGM®; Clonetics) supplemented with 1.5 mM calcium. When 1,25(OH)2D3 (10-7M) was also added to cultures with various concentrations of TGF-β, the ED50 shifted an average of 2-fold less. The presence of TGF-β (10 pg/ml) augmented the potency of 1,25(OH)2D3 by at least 10-fold. In keratinocyte cultures, the antiproliferative effect of the two compounds together is synergistic. In keratinocytes grown for 1 week in the presence of 1,25(OH)2D3 at 10-6M, the TGF-β1 message increased approximately 5-fold. An increase is detected within 2 hours of exposure to 1,25(OH)2D3. There was only a 50% increase in the levels of TGF-β2 and no detection of TGF-β3. When keratinocyte cultures were treated with 1,25(OH)2D3 and neutralizing antibodies to TGF-β, the induced-antiproliferative activity was blocked by more than 50%. The keratinocytes produced more active than latent TGF-β after growth with high doses of 1,25(OH)2D3. © 1992 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041510318
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  • 2
    Electronic Resource
    Electronic Resource
    1,25-Dihydroxycholecalciferol modulates 3H-Thymidine Incorporation in FRTL5 Cells (1992)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 49 (1992), S. 304-309 
    Details
    ISSN: 0730-2312
    Keywords: thyroid follicular cells ; cell proliferation ; cAMP ; protein kinase C ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: 1,25-dihydroxycholecalciferol (1,25(OH)2D3) possesses proliferation and differentiation modulating effects in many cell types in vitro. We studied the effect of 1,25(OH)2D3 on 3H-thymidine incorporation in FRTL5 cells, a cultured rat thyroid follicular cell line. 1,25(OH)2D3 alone at 10-11 and 10-9 M exerted no effect on 3H-thymidine incorporation. However, at 10-7 M, 1,25(OH)2D3 slightly enhanced 3H-thymidine incorporation. In the presence of 5% calf serum, 1,25(OH)2D3 increased 3H-thymidine incorporation induced by calf serum in a dose-dependent manner. 1,25(OH)2D3 also enhanced 3H-thymidine incorporation induced by PMA, an extrinsic stimulator of protein kinase C, without directly affecting PMA-induced protein kinase C translocation. In contrast to the stimulatory effects of 1,25(OH)2D3 on the calf serum and PMA-induced 3H-thymidine incorporation, 1,25(OH)2D3 inhibited the increase in 3H-thymidine incorporation induced by TSH in a dose-dependent manner. This effect of 1,25(OH)2D3 on TSH-induced 3H-thymidine incorporation may be, in part, due to post-cAMP pathways since 1,25(OH)2D3 also inhibited the increase in 3H-thymidine incorporation induced by Bu2cAMP without affecting the TSH-induced increase in cAMP. The stimulatory effect of insulin on 3H-thymidine incorporation, a cAMP-independent process, was also inhibited by 1,25(OH)2D3. We conclude that 1,25(OH)2D3 affects 3H-thymidine incorporation in FRTL5 cells raising the possibility of a physiologic role for 1,25(OH)2D3 in the growth and function of thyroid follicular cells.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240490314
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  • 3
    Electronic Resource
    Electronic Resource
    13-cis-Retinoic acid in chemopreventiion of superficial bladder cancer (1992)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 50 (1992), S. 148-152 
    Details
    ISSN: 0730-2312
    Keywords: 13-cis- retinoic acid ; bladder carcinoma ; chemoprevention ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Animal studies indicate that 13-cis-retinoic acid (CRA) inhibits bladde tumor growth and is effedctive in treating patients with serious dermatologic disorders. A trial of CRA in patients at high risk for rec urrent, TA, T1 tumors was initiated at an experiemtnal dose o f0.5 mg/kg/d in three divided doeses, increasing to 1/mg/kg/d at foru weeks. Treatment of twenty eligible patients lasted for six months with an additional 24 month follow-up period. One patient was later excluded due to toxicity resulting in an early dose a reductionm.Eight patients stopped treatment before three months; of these five, had recurrences swithin three months, one developed pulmonary metastasis, and one developed a T2G3 tumore. Four patients stopped treatment between three and six months; three of them had rercurrences before one year and one had no evidence of disease years. Seven patients completed the course; of these three had recurrences within six months, and three more had recurrences at 8, 15, and 45 months, respectively.Toxicity was nearly universal; cheilosis, conjuctivitis, joint and eye pain, flashing lights, and erythrocyte sedimentation rate (ESR) over 60 were all noted. The lack of positive resutls and the frequency and severity of toxicity led to termination of the study. © 1992 Wiley-Liss, Inc.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240501328
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  • 4
    Electronic Resource
    Electronic Resource
    1993 Keystone symposia on molecular and cellular biology (1992)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 50 (1992), S. 219-219 
    Details
    ISSN: 0730-2312
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240500212
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  • 5
    Electronic Resource
    Electronic Resource
    1α,25-Dihydroxyvitamin D3 modulation in lipid metabolism in established bone marrow-derived stromal cells, MC3T3-G2/PA6 (1992)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 48 (1992), S. 424-430 
    Details
    ISSN: 0730-2312
    Keywords: adipocyte ; adipogenesis ; osteoporosis ; phospholipids ; preadipocyte ; triacylglycerol ; vitamin D derivatives ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: MC3T3-G2/PA6 (PA6) cells established from newborn mouse calvaria are preadipocytic stromal cells, which differentiate into adipocytes in response to glucocorticoids. We examined the effects of 1α,25-dihydroxyvitamin D3[1α,25(OH)2D3] on adipogenesis in PA6 cells were cultured with 10-8 M dexamethasone, adipocytes containing oil red O-positive droplets first appeared on day 7 (3 days after confluence was attained) and the maximal synthesis of neutral lipids occurred on day 12. Simultaneous addition of 1α,25(OH)2D3 at 10-9 M completely blocked this dexamethasone-induced neutral lipid synthesis throughout the 14-day culture period. Dose-response studies of vitamin D3 derivatives showed that 1α,25(OH)2D3 was the most potent in inhibiting neutral lipid synthesis in PA6 cells, followed by 1α-hydroxyvitamin D3, 25-hydroxyvitamin D3 and 24R,25-dihydroxyvitamin D3, in that order. Dexamethasone greatly enhanced incorporation of [14C]-acetic acid into triacylglycerol in PA6 cells. The incorporation was markedly inhibited by the addition of 10-9 M 1α,25(OH)2D3 Instead, 1α,25(QH)2D3 greatly increased incorporation of [14C]-acetic acid into phospholipids, such as phosphatidylcholine and phosphatidylethanolamine, irrespective of the presence or absence of dexamethasone. These results suggest that 1α,25(OH)2D3 modulation of lipid metabolism in bone marrow stromal cells is receptor mediated.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240480411
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  • 6
    Electronic Resource
    Electronic Resource
    240 kDa immunoanalogue of vertebrate α-spectrin occurs in Paramecium cells (1992)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 23 (1992), S. 111-121 
    Details
    ISSN: 0886-1544
    Keywords: anti-α-spectrin immunocytochemistry ; phalloidin staining ; cortex ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Polypeptide of 240 kDa immunorelated to vertebrate α-spectrin was detected in Paramecium cells. The antigen was identified by monospecific antibody directed against α-subunit of spectrin, which was isolated from chicken erythrocytes by affinity and anion-exchange chromatography. Immunoblotting tests demonstrated that the anti-α-spectrin cross reacted with the 240 kDa polypeptide of Paramecium as well as that of various vertebrate cells. In Paramecium, the antigen was detected in cytoskeletal fraction and in contractile extract of the cells. Immuno-fluorescent and immunoelectron microscopy observations revealed cortical localization of α-spectrin immunoanalogue in Paramecium. The label was distinctly seen on the whole surface of trichocyst tips. The antigen was also distributed close to the inner alveolar membrane forming a regular, continuous, lattice-like structure. When stained with rhodamine-phalloidin, Paramecium cells displayed a similar fluorescent network, which underlay contours of cortical units. Basal bodies of cilia were labeled with phalloidin as well. Detection of α-spectrin immunoanalogue in Paramecium cortex may provide a new insight into arrangement of cortical elements in this organism. © 1992 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970230204
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  • 7
    Electronic Resource
    Electronic Resource
    A cell attachment peptide from human C-reactive proteinPresented in part at the 31st annual meeting of the American Society for Cell Biology, December 12, 1991, Boston, MA. (Abstract #2564). (1992)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 50 (1992), S. 83-92 
    Details
    ISSN: 0730-2312
    Keywords: acute phase reactant ; serum amyloid P-component ; tissue repair ; extracellular matrix proteins ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The serum acute phase reactant, C-reactive protein (CRP), is selectively deposited at sites of tissue damage and degraded by neutrophils into biologically active peptides. A synthetic peptide corresponding to residues 27-38 present in each of the five identical subuints of CRP mediated cell attachment activity in vitro. Although the CRP-derived peptide contains a Tuftsin (TKPR)-like sequence at its amino-terminus, the Tuftsin tetrapeptide itself, as well as several synthetic peptides of CRP, failed to inhibit the cell-attachment activity to the CRP-derived peptide. Peptides containing the sequences responsible for the cell attachment activity of the extracellular matrix proteins, fibronectin (Fn) and laminin, failed to inhibit the CRP-derived peptide cell attachment activity. However the addition of the RGDS and RGDSPASSLP cell-binding peptides of Fn to cells enhanced attachment to the active peptide from CRP. In the converse experiment, the cell-binding peptide of CRP did not influence cell attachment to Fn or laminin. A peptide corresponding to the same stretch of amino acid residues within the homologous Pentraxin, serum amyloid P-component (SAP), displayed nearly identical cell- attachment activity. Several monoclonal antibodies (mAb) specific for the CRP-derived cell-binding peptide neutralized its cell-attachment activity. These mAbs reacted with intact CRP and neutralized the cell-binding activity of CRP itself. The findings suggest that a peptide with cell-binding activity could be generated from the breakdown of the CRP and then contribute directly to cellular events leading to tissue repair. © 1992 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240500113
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  • 8
    Electronic Resource
    Electronic Resource
    A combined pseudoreplica-immunochemical technique for research and diagnostic Virology (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 21 (1992), S. 59-64 
    Details
    ISSN: 1059-910X
    Keywords: Vesicular stomatitis virus ; Cytomegalovirus ; Herpes simplex virus ; Human parvovirus B19 ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Pseudoreplica and immunochemical techniques were combined in a single protocol for identification of virus in research and clinical specimens. Stock preparations of vesicular stomatitis virus (VSV), cytomegalovirus (CMV), and herpes simplex virus (HSV) were used to develop the technique. Traditional pseudoreplicas of viral stock solutions were prepared but not negatively stained. The virus was then immunolabeled in two stages. Virus-specific polyclonal antisera were used in the first stage; colloidal gold congugated antibodies were used as indicator antibody in the second stage. After immunolabeling, the grids were negatigvely stained. As a demonstration of the clinical usefulness of this approach, it was employed to antenatally identify human parvovirus B19 particles in ascites from a 22 week gestational fetus with nonimmune hydrops fetalis. The combined pseudoreplica-immunochemical approach offers several advantages over both the pseudoreplica and immunochemical methods when used in isolation. Advantages include relative purification of samples, concentration of virus, morphological preservation, and enhanced diagnostic specificity.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070210109
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  • 9
    Electronic Resource
    Electronic Resource
    A comparative overview of mammalian fertilization. Edited by Bonnie S. Dunbar and Michael G. O'Rand, Plenum Publishing Corp., New York, 1991, 457 pp, $95 (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 32 (1992), S. 409-409 
    Details
    ISSN: 1040-452X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080320418
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  • 10
    Electronic Resource
    Electronic Resource
    A comparison between in vitro fertilization and microinjection of immobilized spermatozoa from bulls producing spermatozoa with defects (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 33 (1992), S. 489-491 
    Details
    ISSN: 1040-452X
    Keywords: Oocytes ; Cattle ; Sperm ; Fertility ; Microinjection ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The objectives of this study were to compare the fertilization rate of bovine in vitro matured oocytes by in vitro fertilization (IVF) and by microinjection of a single spermatozoon (MI) and to relate these rates with fertility reported for these bulls in artificial breeding. Bull A (Holstein) had a nonreturn rate of 75%. Semen from this bull is routinely used in our standard IVF procedure. Bull B (Ayrshire), used regularly in artificial breeding and related to bull D, had a nonreturn rate of 69.2%. Bull C (Brown Swiss), with a chromosomal translocation and trisomy, achieved a nonreturn rate of 42%. Bull D (Ayrshire) produced nonmotile spermatozoa (SPZ) and had an abnormality described as “tail stump defect.” No pregnancies sired by bull D have been reported. Oocytes were either fertilized in vitro by capacitated SPZ or by microinjection of a single immobilized SPZ into the ooplasma. SPZ were treated with 0.1 μM A23187 and used for IVF. For microinjection SPZ were cocultured for 5 h with bovine oviduct epithelial cells (BOEC) and then immobilized by freezing and thawing twice without cryoprotectant. A single batch of killed SPZ (stored at - 25°C) was used for all microinjections. All oocytes were cultured in Medium 199 for 22 h at 39°C and subsequently fixed, stained, and examined for evidence of fertilization (i.e., female and male pronucleus formation, SPZ decondensation). Fertilization rates following IVF with semen from bulls A, B, C, and D were 80%, 54%, 1%, and 2%, and following microinjection were 39%, 22%, 21%, and 34%, respectively. © 1992 Wiley-Liss, Inc.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080330416
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