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  • Spektralphotometrie, Atomabsorption  (19)
  • Immunocytochemistry  (17)
  • Springer  (36)
  • 1975-1979  (36)
  • 1978  (36)
Collection
Keywords
Publisher
  • Springer  (36)
Years
  • 1975-1979  (36)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Phytochrome photoreversibility: Empirical test of the hypothesis that it varies as a consequence of pigment compartmentation (1978)
    Springer
    Planta 141 (1978), S. 129-134 
    Details
    ISSN: 1432-2048
    Keywords: Avena ; Immunocytochemistry ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome of oat (Avena sativa L., cv. Garry) coleoptile cells in the red-light-absorbing form, Pr, is diffusely distributed while after conversion to the far-red-light-absorbing form, Pfr, it is observed only in very small areas within the cell. Comparison of phytochrome photoversibility measurements to the distribution of the pigment within the cell indicates that the spectral assay is not influenced by the observed compartmentalization of the chromoprotein. However, the observed compartmentalization of phytochrome is correlated with a loss in spectrophotometrically detectable Pr.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387878
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  • 2
    Electronic Resource
    Electronic Resource
    Anreicherung und Bestimmung von Blei in Boden- und Sedimentaufschlüssen und -extrakten mit der Flammenatomabsorption (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 291 (1978), S. 213-216 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Blei in Boden, Sedimenten ; Spektralphotometrie, Atomabsorption ; Flamme, Reextraktion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung In Vorversuchen wurde gezeigt, da\ beim Aufbewahren der organischen APDC/MIBK-Extrakte starke Bleiverluste auftraten. Daher war es notwendig, das Blei mit SalpetersÄure in die wÄ\erige Phase zu reextrahieren. Wiederfindungsversuche bestÄtigten, da\ Extraktion und Reextraktion quantitativ verlaufen. Das Blei wird dabei um den Faktor fünf angereichert. Es werden mittlere Standardabweichungen für vier Konzentrationsbereiche angegeben. Als Nachweisgrenze wurde eine Konzentration von 0,16 mg Pb/l gefunden, die durch die Anreicherung auf ca. 0,03 mg Pb/l gesenkt wird. Arbeitsvorschriften für das Verfahren sind angegeben.
    Notes: Summary Preliminary investigations have shown, that considerable losses of lead occur during storage of the organic APDC/MIBK-extracts. Therefore, it was necessary to reextract lead into the aqueous phase by nitric acid. Investigations of recoveries confirmed, that extraction and reextraction of lead were quantitative. In this way the concentration of lead is increased by a factor of five. Mean standard deviations for four concentration ranges are given. A detection limit of 0.16 mg of Pb/l is obtained, which is lowered by the preceding enrichment to about 0.03 mg of Pb/l. Details of the procedure are given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00480692
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  • 3
    Electronic Resource
    Electronic Resource
    Zur AAS-Bestimmung von Quecksilber in Zahnwurzeln und Kieferknochen (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 293 (1978), S. 141-144 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Quecksilber in Zähnen, Knochen ; Spektralphotometrie, Atomabsorption ; HNO3/HF-Aufschluß, Kaltdampfverfahren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es wird ein Verfahren zur Bestimmung von Quecksilber in Zahnwurzeln und Kieferknochen beschrieben. Die homogenisierten Proben werden in Teflonbomben unter Druck mit einem Gemisch von HNO3/HF aufgeschlossen. Die Quecksilberbestimmung erfolgt mit einem Atomabsorptionsspektrophotometer nach dem Kaltdampfverfahren. Unter Einsatz von 200 mg Probe können Quecksilbergehalte bis zu 0,1 μg/g bestimmt werden. Die relativen Standardabweichungen liegen im Arbeitsbereich von 10–100 ng Quecksilber zwischen 2,7 und 5,6 %. Es wurden Quecksilbergehalte von 〈0,1–187 μg/g in den Proben gefunden.
    Notes: Summary The homogenized samples are decomposed with a mixture of HNO3/HF in teflon vessels under pressure. The determination of mercury is carried out with an atomic absorption spectrophotometer by means of the cold-vapour technique. The limit of detection is 0.1 μg/g for a 200 mg sample. A relative standard deviation of 2.7–5.6% is achieved in the working range of 10–100 ng mercury. Mercury contents of 〈0.1–187 μg/g were found in the samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00486994
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  • 4
    Electronic Resource
    Electronic Resource
    Extraction and atomic-absorption spectrometric determination of traces of bismuth with zinc dibenzyldithiocarbamate in aluminium alloys and solder alloys (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 282-284 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Wismut in Aluminiumlegierungen, Lötlegierungen ; Spektralphotometrie, Atomabsorption ; Zinkdibenzyldithiocarbamat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A sensitive method for the extraction and atomic-absorption spectrometric determination of bismuth with zinc dibenzyldithiocarbamate (ZnDBC) has been developed for aluminium alloys and solder alloys. Bismuth is extracted from relatively strong acidic solution into methyl isobutyl ketone (MIBK) as BiDBC complex and its concentration is determined by spraying the MIBK extract into an air-acetylene flame. Iron and copper up to 1 mg resp. 100 μg did not interfere with the extraction of bismuth in the range of 0–80 μg. The limit of detection was about 0.12 ppm in MIBK.
    Notes: Zusammenfassung Ein empfindliches Verfahren zur Wismutbestimmung mit Zinkdibenzyldithiocarbamat wurde ausgearbeitet. Wismut wird dabei aus relativ stark saurer Lösung als Bi-DBC-Komplex mit Methylisobutylketon extrahiert und zur Messung der Extrakt direkt in die Luft-Acetylen-Flamme gesprüht. Bis zu 1 mg Fe und 100 μg Cu stören die Bestimmung von 0–80 μg Bi nicht. Die Nachweisgrenze beträgt 0,12 ppm in Methylisobutylketon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493696
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  • 5
    Electronic Resource
    Electronic Resource
    Comparative study of sodium borohydride tablet and tin(II) chloride reducing systems in the determination of mercury by atomic-absorption spectrophotometry (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 273-277 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Quecksilber ; Spektralphotometrie, Atomabsorption ; Kaltdampf, Vergleich der Reduktion mit NaBH4-Tablette oder SnCl2-Lösung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary An atomic-absorption spectrophotometric method for the rapid and precise determination of mercury has been studied by using the sodium borohydride tablet reduction and cold-vapour atomicabsorption procedure coupled with amalgamation on gold. The comparison of the sodium borohydride tablet and tin(II) chloride solution reducing systems are described in detail. The use of the sodium borohydride tablet wrapped in a wafer sheet is very simple and gives more precise results. From the comparison of the effect of diverse ions, it is obvious that both reducing systems have their merits and demerits.
    Notes: Zusammenfassung Ein atomabsorptions-spektralphotometrisches Kaltdampf-Verfahren zur schnellen Quecksilberbestimmung wird beschrieben, das auf der Reduktion mit Hilfe der Natriumborhydridtablette und der Amalgamierung mit Gold beruht. Der Vergleich mit der Zinn(II)-Chloridmethode wird diskutiert. Genaue Ergebnisse werden durch einfaches Einhüllen der Reduktionstablette in ein Waffelblatt erzielt, wodurch der Reaktionsbeginn verzögert wird. In bezug auf Störelemente ergeben sich für beide Verfahren Vor- und Nachteile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493694
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  • 6
    Electronic Resource
    Electronic Resource
    Bestimmung von Blei in kleinen Proben der marinen Diatomee Skeletonema costatum (Grev.) Cleve durch flammenlose Atom-Absorptions-Spektrometrie nach Flußsäureveraschung (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 278-281 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Blei in Algen, Diatomeen ; Spektralphotometrie, Atomabsorption ; flammenlos, Störungen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es wird eine nasse HF-HClO4Veraschungsmethode von biologischem Material in offenen PTFE-Reagensgläsern beschrieben. In anschließenden flammenlosen AAS-Messungen mit Hilfe der Deuterium-Untergrundkompensation und des automatischen Probenwechslers AS-1 konnte gezeigt werden, daß Silicium vollständig entfernt worden, kein meßbarer Matrix-Effekt festzustellen war und die Bleikonzentrationsbestimmung durch eine Eichgerade und durch Standard-Addition zu gleichen Werten führte. Die Methode ist mit einem Variations-Koeffizient von 0,45% behaftet.
    Notes: Summary A wet HF-HClO4 decomposition method for biological material in open PTFE-test-tubes is described. Flameless AAS measurements with the aid of deuterium background compensation using the auto sampling system AS-1 showed that Si was completely eliminated by the decomposition, that no measurable matrix effect could be detected, and that the values found for the lead concentration by calibration curve and standard addition were identical. The coefficient of variation for the method was V=0.45%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493695
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  • 7
    Electronic Resource
    Electronic Resource
    Determination of organosilicon compounds in water by atomic absorption spectroscopy (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 362-364 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Siliciumorganoverbindungen in Wasser ; Spektralphotometrie, Atomabsorption ; Extraktionsverfahren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary An extraction technique is described for the determination of ppm concentrations of both hydrophobic and hydrophilic methylsiloxane compounds in water, based on a single extraction with a mixed solvent of (1∶1) l-pentanol and methyl isobutyl ketone followed by the determination of the silicone compounds extracted into the mixed solvent by atomic absorption spectroscopy. The method is applicable over the concentration range 0.3–30 ppm Si. Standard deviation in the case of 10 ppm Si is 0.313.
    Notes: Zusammenfassung Zur Bestimmung von ppm-Konzentrationen an hydrophoben und hydrophilen Methylsiloxanverbindungen im Wasser wird ein Extraktionsverfahren beschrieben. Hierbei wird nach einfacher Extraktion mit l-Pentanol/Methylisobutylketon (1∶1) das Silicium im Extrakt mit Hilfe der AAS bestimmt. Der erfaßbare Konzentrationsbereich liegt bei 0,3–30 ppm Si. Die Standardabweichung beträgt bei 10 ppm Si 0,313.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00481552
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  • 8
    Electronic Resource
    Electronic Resource
    Localization of thyrotropin (TSH) in the dog pituitary gland (1978)
    Springer
    Cell & tissue research 186 (1978), S. 399-412 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Dog ; Pars distalis ; Thyrotropin (TSH) ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using the immunoperoxidase technique and antisera to the specific beta (β) subunits of bovine and rat TSH1, selective immunocytochemical staining was localized in a specific cell population in the pars distalis of the dog pituitary gland. These TSH cells were found to be positive to aldehyde fuchsin, alcian blue, periodic acid-Schiff (PAS) and aniline blue. With the performic acidalcian blue (pH 0.2) -PAS-orange G procedure these cells stained blue-purple, demonstrating FSH/LH cells (blue or turquoise), ACTH/MSH cells (redpurple) and PRL cells (orange-red). The TSH cells were further differentiated from other functional cell types of the pars distalis on the basis of their typical cytological features, intraglandular distribution and by immunocytochemical double staining. In the pars distalis of adult male dogs the TSH cells were mostly shown to be smaller in size and less numerous than in bitches in the anestrous phase of the sexual cycle. Moreover, cytological alterations in the immunoreactive thyrotrophs in the pituitary of male and female dogs generally paralleled the spontaneous changes in thyroid function associated with thyroid atrophy and/or pituitary insufficiency, and thyroid hyperplasia or goiter. In conclusion, because of their specificity and high potency, the antisera to the β-subunits of bovine and rat TSH represent an effective tool for the selective immunocytochemical localization of TSH in the dog pituitary. This allows the study of the morphology and function of TSH cells under different physiological, pathological and experimental conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224930
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  • 9
    Electronic Resource
    Electronic Resource
    Immunocytochemical identification of an LHRH-producing system originating in the preoptic nucleus of the duck (1978)
    Springer
    Cell & tissue research 188 (1978), S. 99-106 
    Details
    ISSN: 1432-0878
    Keywords: LHRH-neurosecretion ; Avian hypothalamus ; Vasotocin neurosecretion ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A fluorescent technique applying specific LHRH and vasotocin antisera was used for the immunocytochemical localization of the respective neurosecretory systems in the hypothalamus of gonadectomized, testosteronetreated and/or serotonin injected male domestic ducks. An immunoreactive (IR) LHRH-producing system, with perikarya located in the preoptic nucleus, could be traced through the ventral hypothalamus down to the external layer of the rostral and caudal ME, in close vicinity to the hypophysial portal system. An IR-vasotocin system originating in the paraventricular and supraoptic nuclei ran through the ventral hypothalamus, but terminated in (i) the external layer of the rostral ME, and (ii) in the posterior lobe of the hypophysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220517
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  • 10
    Electronic Resource
    Electronic Resource
    Immunocytochemical study of the hypothalamo-neurohypophysial system (1978)
    Springer
    Cell & tissue research 188 (1978), S. 119-132 
    Details
    ISSN: 1432-0878
    Keywords: Neurophysin ; Paraventricular nucleus ; Supraoptic nucleus ; Sheep ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An antiserum cross-reactive against ovine neurophysins-I-II and -III has been used in conjunction with the immunoperoxidase histochemical procedure to localize the cells of the sheep paraventricular (PVN) and supraoptic nuclei (SON). In order to describe the topographical distribution of the SON and PVN a study was made on the serial sections cut (a) transversely from rostral to caudal positions and (b) sagittally from lateral to medial positions of the hypothalamus. The cells of the SON, when examined in the transverse aspect, extended approximately 1900 μ caudally and when examined in the sagittal plane were contained within a lateral-medial distance of 4830 μ. In each case the SON cells lay adjacent to the optic chiasm. As sections were cut transversely, the cells of the PVN first appeared in a rostral position defined as 0 μ and close to the ventral lining of the third ventricle. This general ventral and ventro-lateral distribution of cells maintained up to a caudal distance of approximately 840 μ. From positions 1260–2310 μ there was a dramatic dorsal shift of the PVN cells which by this time had also extended laterally. The total rostral-caudal distance occupied by the PVN cells was 3150 μ. That the lateral-medial distance occupied by the PVN was small (1050 μ) was determined on examining the magnocellular nuclei in sagittal section.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220519
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