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  • Biochemistry and Biotechnology  (39)
  • Analytical Chemistry and Spectroscopy
  • Wiley-Blackwell  (39)
  • 1960-1964  (39)
  • 1961  (39)
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  • Wiley-Blackwell  (39)
Years
  • 1960-1964  (39)
Year
  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 95-109 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A production model consisting of one or more exponential decay functions is proposed to represent the rate of accumulation of a product per unit weight of cell. This, together with a cell age distribution function, is used to describe the product accumulation in a fermentation process.This postulate offers a unified concept applicable to both batch and continuous processes as well as problems arising from environmental changes. The idea is compatible with the current understanding of microbial physiology. It offers an interpretation for the controversy over cell-product ratios observed in some batch and continuous fermentations when treated as conventional chemical reaction. The proposed postulate fits reasonably well with the lysine and lactic acid processes.
    Additional Material: 6 Ill.
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 1-6 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An empirical relationship of oxygen transfer rate and mould viscosity is discussed, which leads to equations for predicting the OTR of mould fermentations.
    Additional Material: 4 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 29-39 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Laboratory scale experiments have been carried out with sulphate-reducing bacteria growing under anaerobic conditions to investigate the removal of iron from iron-containing solutions. The complete removal of iron, as the insoluble sulphide, has been achieved from both ferrous and ferrous-ferric sulphate solutions. It has been found that under certain conditions a magnetic iron sulphide is produced. This aggregates under the influence of a magnetic field and settles at a rate of some 30 in./min. The magnetic sulphide has only been obtained from intermittent continuous cultures. With the system employed, rates of removal of up to 150 mg of iron per litre of culture vessel per hour have been obtained.
    Additional Material: 1 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 41-50 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method has been developed for the recovery of enterotoxin from bacterial culture supernatants of staphylococcus strain S-6 by use of the ion-exchange resin Amberlite XE-64 (IRC-50). The enterotoxin from 200 ml of bacterial culture supernatant diluted with one volume of water and adjusted to pH 6 can be removed with 1 g of resin pretreated with 0·02 M sodium phosphate, pH 6·2. The enterotoxin is recovered from the resin with 0·2 M sodium phosphate, pH 6·2, with subsequent precipitation with EtOH at -10°C, dialysis, and freeze drying. Partially purified preparations containing 1 emetic dose per 50μg were obtained.
    Additional Material: 4 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 115-117 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Tab.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 119-123 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An analytical method has been devised for the rapid determination of prednisolone produced in a fermentative oxidation process. It allows frequent checking of concentrations of the prednisolone formed during the oxidation of hydrocortisone.The estimation of prednisolone in the presence of hydrocortisone is based upon the diversity of colour shown by the chromogens produced from these steroids by sulphuric acid and ethanol. Using the optimal reagent mixture and conditions, the interference of hydrocortisone is negligible. The steroids are extracted from the fermentation broth with ethyl acetate. One test takes about 20 min.
    Additional Material: 3 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 161-174 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The preparation of soybeans for fermentation is a very important part of the process for making miso, a traditional Japanese food. In general, soybeans from the United States are smaller and more pigmented than Japanese soybeans. Twenty-eight American varieties, four Japanese varieties and one Chinese variety have been studied with respect to absorption of water and cooking. A variety of soaking and cooking conditions have been investigated. Some U.S. varieties absorb water and cook unevenly. Unevenness in cooking is not associated with the size of the beans within a variety.
    Additional Material: 1 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 199-218 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A process is described for the production of a specific-molecular-weight dextran, e.g. clinical dextran, by using a modified form of controlled synthesis. The synthesis is carried out directly in a medium containing appropriate amounts of sucrose and low-molecular-weight dextran after inoculation with a culture of actively growing bacteria. The importance of an inoculum substantially freed from substances affecting the synthesis of dextran is demonstrated. The technique appears to offer a decided advantage over other well-known procedures. Technical details of the process and equipment are given.
    Additional Material: 10 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 219-233 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Several strains of Pseudomonas, Nocardia and incompletely identified soil isolates have been grown in a mineral salts plus hydrocarbon medium, and the fatty acids produced by the organisms have been isolated, identified and estimated. The results of an estimation of the percentage conversion to these acids under varying experimental conditions is discussed in relation to the metabolic systems involved. Some indication has been obtained that the hydrocarbon breakdown pathway by these organisms is that of ω oxidation followed by β oxidation. Preliminary experiments carried out with one strain of organism, Pseudomonas aeruginosa 5940, indicate that there may be some difference in the utilization of odd- and even-chain hydrocarbons by this organism. An improvement of 3 ·8-fold was obtained by using nitrate instead of ammonium nitrogen; 13-fold by using continuous instead of batch operation; and 8-fold by the use of liquid instead of solid paraffins, giving a total improvement of yield of 400-fold.
    Additional Material: 2 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 175-180 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A simple procedure for the disintegration of yeast cells, by which litre quantities of cell extract (approximately 11 per cent dry weight) may be obtained, is described. The fresh yeast cake is treated with dry ice in a high speed electric homogenizer after which it is left to thaw out at 0°. No addition of buffer has to be made, and the cell juice obtained after centrifugation of the resulting slurry appears to contain different subcellular particles and various enzymes in a relatively native state.When the extract is incubated at higher temperatures, proteolysis will rapidly change the electrophoretic and enzymatic properties, which indicates that procedures involving autolysis may be dangerous when information about the chemical composition of the native enzymes is desired.
    Additional Material: 3 Ill.
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