Publication Date:
2024-05-24
Description:
Contaminants of emerging concern and ocean changes are key environmental stressors for marine species with possibly synergistic, but still unexplored, deleterious effects. In the present study the influence of a simulated ocean acidification scenario (pH = 7.6) was investigated on metabolism and sub-lethal effects of carbamazepine, CBZ (1 µg/L), chosen as one of the most widely diffused pharmaceuticals in marine organisms. A multidisciplinary approach was applied on mussels, M. galloprovincialis, integrating measurement of drug bioaccumulation with changes in the whole transcriptome, responsiveness of various biochemical and cellular biomarkers including immunological parameters, lipid and oxidative metabolism, onset of genotoxic effects. Chemical analyses revealed a limited influence of hypercapnia on accumulation and excretion of CBZ, while a complex network of biological responses was observed in gene expression profile and functional changes at cellular level. The modulation of gamma-aminobutyric acid (GABA) pathway suggested similarities with the Mechanism of Action known for vertebrates: immune responses, cellular homeostasis and oxidative system represented the processes targeted by combined stressors. The overall elaboration of results through a quantitative Weight of Evidence model, revealed clearly increased cellular hazard due to interactions of CBZ with acidification compared to single stressors.
Keywords:
Acetylcholinesterase activity, per protein mass; Acetylcholinesterase activity, per protein mass, standard error; Acyl-CoA oxidase activity, per protein mass; Acyl-CoA oxidase activity, per protein mass, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbamazepine, per dry mass; Carbamazepine, standard deviation; Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Catalase activity, per protein mass; Catalase activity, per protein mass, standard error; Coast and continental shelf; Containers and aquaria (20-1000 L or 〈 1 m**2); DNA fragmentation; DNA fragmentation, standard error; Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Glutathione, total, per unit wet mass; Glutathione, total, per unit wet mass, standard error; Glutathione reductase, per protein mass; Glutathione reductase, per protein mass, standard error; Glutathione S-transferase, activity per protein mass; Glutathione S-transferase, activity per protein mass, standard error; Granulocytes/Hyalinocytes ratio; Granulocytes/Hyalinocytes ratio, standard error; Hemocytes lysosomal membranes stability; Hemocytes lysosomal membranes stability, standard error; Hemocytes micronuclei frequency; Hemocytes micronuclei frequency, standard error; Immunology/Self-protection; Laboratory experiment; Lipofuscin; Lipofuscin, standard error; Malondialdehyde, per wet mass; Malondialdehyde, per wet mass, standard error; Mediterranean Sea; Mollusca; Mytilus galloprovincialis; Neutral lipids; Neutral lipids, standard error; OA-ICC; Ocean Acidification International Coordination Centre; Organic toxins; Other metabolic rates; Other studied parameter or process; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; Phagocytosis; Phagocytosis, standard error; Potentiometric; Potentiometric titration; Registration number of species; Salinity; Salinity, standard deviation; Sample code/label; Selenium dependent glutathione peroxidases activity, per protein mass; Selenium dependent glutathione peroxidases activity, per protein mass, standard error; Single species; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Total glutathione peroxidases activity, per protein mass; Total glutathione peroxidases activity, unit per protein mass, standard error; Total oxyradical scavenging capacity against hydroxyl radicals, unit per protein mass; Total oxyradical scavenging capacity against hydroxyl radicals, unit per protein mass, standard error; Total oxyradical scavenging capacity against peroxyl radicals, unit per protein mass; Total oxyradical scavenging capacity against peroxyl radicals, unit per protein mass, standard error; Total oxyradical scavenging capacity against peroxynitrite, unit per protein mass; Total oxyradical scavenging capacity against peroxynitrite, unit per protein mass, standard error; Treatment; Type; Uniform resource locator/link to reference
Type:
Dataset
Format:
text/tab-separated-values, 331 data points
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