ALBERT

Description: It is essential to predict the impact of elevated PCO2 on marine organisms and habitats to anticipate the severity and consequences of future ocean chemistry change. Despite the importance of carry-over effects in the evolutionary history of marine organisms, few studies have considered links between life-history stages when determining how marine organisms will respond to elevated PCO2, and none have considered the link between adults and their offspring. Herein, we exposed adults of wild and selectively bred Sydney rock oysters, Saccostrea glomerata to elevated PCO2 during reproductive conditioning and measured the development, growth and survival response of their larvae. We found that elevated PCO2 had a negative impact on larvae of S. glomerata causing a reduction in growth, rate of development and survival. Exposing adults to elevated PCO2 during reproductive conditioning, however, had positive carry-over effects on larvae. Larvae spawned from adults exposed to elevated PCO2 were larger and developed faster, but displayed similar survival compared with larvae spawned from adults exposed to ambient PCO2. Furthermore, selectively bred larvae of S. glomerata were more resilient to elevated PCO2 than wild larvae. Measurement of the standard metabolic rate (SMR) of adult S. glomerata showed that at ambient PCO2, SMR is increased in selectively bred compared with wild oysters and is further increased during exposure to elevated PCO2. This study suggests that sensitive marine organisms may have the capacity to acclimate or adapt to elevated PCO2 over the next century and a change in energy turnover indicated by SMR may be a key process involved.

Description: The activities of proteinases, lipases/esterases and citrate synthase of Calanus finmarchicus copepodites (CV) were analysed. Analysis was performed at 30°C for copepods from seven stations (126-9, 127-17, 131-17, 133-6, 134-19, 135-16, 136-8). In addition, thermal profiles (5-50°C) of these enzymes were analysed for copepods from 3 stations (127-17, 133-6, 135-16). C. finmarchicus of station 127-19 have been acclimated on board to two different temperatures (4 and 15°C) for two weeks. Thermal profiles (5-60°C) of lipases/esterases and proteinases of adult females from each treatment were analysed. Groups of 10 individuals were used to prepare enzyme extracts for analysis. From each station/treatment, three groups were analysed, each of which was measured in triplicates. The activity of proteinases was determined photometrically after Saborowski et al. (2004, hdl:10013/epic.20836), modified after Kreibich et al. (2008, doi:10.1007/s10152-008-0112-0). Azocasein was used as substrate. The lypolytic activity of lipases and esterases in the extract was analysed fluorometrically after Knotz et al. (2006, doi:10.1016/j.cbpa.2006.07.019) using 4-methylumbelliferyl butyrate as substrate. Citrate synthase activity was analysed photometrically after Stitt (1984) modified by Saborowski and Buchholz (2002) with oxaloacetic acid as substrate. For detailed description please contact the author.

Description: In Gobabeb, Namibia SWD, LWD and DIR are each measured redundant with two instruments of the same make (not in this data base). The differences between the pairs are used in the quality control. This is done manually by inspecting plots of half-day diurnal courses of the pairs and their differences. Values are removed mostly in the morning due to daily cleaning. Other reasons for larger differences are birds, insects, or people at the station. There are regular fog events varying in frequency over the year. Usually, the fog appears in the second half of the night and disappears a few hours after sunrise. The case temperatures of pyrgeometers practically never drop below dewpoint but there can be water deposition of the dome.

Description: In Gobabeb, Namibia SWD, LWD and DIR are each measured redundant with two instruments of the same make (not in this data base). The differences between the pairs are used in the quality control. This is done manually by inspecting plots of half-day diurnal courses of the pairs and their differences. Values are removed mostly in the morning due to daily cleaning. Other reasons for larger differences are birds, insects, or people at the station. There are regular fog events varying in frequency over the year. Usually, the fog appears in the second half of the night and disappears a few hours after sunrise. The case temperatures of pyrgeometers practically never drop below dewpoint but there can be water deposition of the dome.

Description: In Gobabeb, Namibia SWD, LWD and DIR are each measured redundant with two instruments of the same make (not in this data base). The differences between the pairs are used in the quality control. This is done manually by inspecting plots of half-day diurnal courses of the pairs and their differences. Values are removed mostly in the morning due to daily cleaning. Other reasons for larger differences are birds, insects, or people at the station. There are regular fog events varying in frequency over the year. Usually, the fog appears in the second half of the night and disappears a few hours after sunrise. The case temperatures of pyrgeometers practically never drop below dewpoint but there can be water deposition of the dome.

Description: In Gobabeb, Namibia SWD, LWD and DIR are each measured redundant with two instruments of the same make (not in this data base). The differences between the pairs are used in the quality control. This is done manually by inspecting plots of half-day diurnal courses of the pairs and their differences. Values are removed mostly in the morning due to daily cleaning. Other reasons for larger differences are birds, insects, or people at the station. There are regular fog events varying in frequency over the year. Usually, the fog appears in the second half of the night and disappears a few hours after sunrise. The case temperatures of pyrgeometers practically never drop below dewpoint but there can be water deposition of the dome.

Description: In Gobabeb, Namibia SWD, LWD and DIR are each measured redundant with two instruments of the same make (not in this data base). The differences between the pairs are used in the quality control. This is done manually by inspecting plots of half-day diurnal courses of the pairs and their differences. Values are removed mostly in the morning due to daily cleaning. Other reasons for larger differences are birds, insects, or people at the station. There are regular fog events varying in frequency over the year. Usually, the fog appears in the second half of the night and disappears a few hours after sunrise. The case temperatures of pyrgeometers practically never drop below dewpoint but there can be water deposition of the dome.

Description: In Gobabeb, Namibia SWD, LWD and DIR are each measured redundant with two instruments of the same make (not in this data base). The differences between the pairs are used in the quality control. This is done manually by inspecting plots of half-day diurnal courses of the pairs and their differences. Values are removed mostly in the morning due to daily cleaning. Other reasons for larger differences are birds, insects, or people at the station. There are regular fog events varying in frequency over the year. Usually, the fog appears in the second half of the night and disappears a few hours after sunrise. The case temperatures of pyrgeometers practically never drop below dewpoint but there can be water deposition of the dome.

Description: In Gobabeb, Namibia SWD, LWD and DIR are each measured redundant with two instruments of the same make (not in this data base). The differences between the pairs are used in the quality control. This is done manually by inspecting plots of half-day diurnal courses of the pairs and their differences. Values are removed mostly in the morning due to daily cleaning. Other reasons for larger differences are birds, insects, or people at the station. There are regular fog events varying in frequency over the year. Usually, the fog appears in the second half of the night and disappears a few hours after sunrise. The case temperatures of pyrgeometers practically never drop below dewpoint but there can be water deposition of the dome.