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  • National Academy of Sciences  (19,456)
  • 2005-2009  (19,456)
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  • 11
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    National Academy of Sciences
    In:  PNAS Proceedings of the National Academy of Sciences of the United States of America, 106 . pp. 20602-20609.
    Publication Date: 2019-09-23
    Description: Throughout Earth's history, the oceans have played a dominant role in the climate system through the storage and transport of heat and the exchange of water and climate-relevant gases with the atmosphere. The ocean's heat capacity is ≈1,000 times larger than that of the atmosphere, its content of reactive carbon more than 60 times larger. Through a variety of physical, chemical, and biological processes, the ocean acts as a driver of climate variability on time scales ranging from seasonal to interannual to decadal to glacial–interglacial. The same processes will also be involved in future responses of the ocean to global change. Here we assess the responses of the seawater carbonate system and of the ocean's physical and biological carbon pumps to (i) ocean warming and the associated changes in vertical mixing and overturning circulation, and (ii) ocean acidification and carbonation. Our analysis underscores that many of these responses have the potential for significant feedback to the climate system. Because several of the underlying processes are interlinked and nonlinear, the sign and magnitude of the ocean's carbon cycle feedback to climate change is yet unknown. Understanding these processes and their sensitivities to global change will be crucial to our ability to project future climate change.
    Type: Article , PeerReviewed
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  • 12
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    National Academy of Sciences
    In:  Proceedings of the National Academy of Sciences of the United States of America (PNAS), 103 (31). pp. 11647-11652.
    Publication Date: 2019-09-23
    Description: The green lineage is reportedly 1,500 million years old, evolving shortly after the endosymbiosis event that gave rise to early photosynthetic eukaryotes. In this study, we unveil the complete genome sequence of an ancient member of this lineage, the unicellular green alga Ostreococus tauri (Prasinophyceae), This cosmopolitan marine primary producer is the world's smallest free-living eukaryote known to date. Features likely reflecting optimization of environmentally relevant pathways, including resource acquisition, unusual photosynthesis apparatus, and genes potentially involved in C4 photosynthesis, were observed, as was downsizing of many gene families. Overall, the 12.56-Mb nuclear genome has an extremely high gene density, in part because of extensive reduction of intergenic regions and other forms of compaction such as gene fusion. However, the genome is structurally complex. It exhibits previously unobserved levels of heterogeneity for a eukaryote. Two chromosomes differ structurally from the other eighteen. Both have a significantly biased G+C content, and, remarkably, they contain the majority of transposable elements. Many chromosome 2 genes also have unique codon usage and splicing, but phylogenetic analysis and composition do not support alien gene origin. In contrast, most chromosome 19 genes show no similarity to green lineage genes and a large number of them are specialized in cell surface processes. Taken together, the complete genome sequence, unusual features, and downsized gene families, make O. tauri an ideal model system for research on eukaryotic genome evolution, including chromosome specialization and green lineage ancestry. © 2006 by The National Academy of Sciences of the USA.
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  • 13
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    National Academy of Sciences
    In:  PNAS Proceedings of the National Academy of Sciences of the United States of America, 106 (31). pp. 12788-12793.
    Publication Date: 2016-11-14
    Description: Understanding the ecological impacts of climate change is a crucial challenge of the twenty-first century. There is a clear lack of general rules regarding the impacts of global warming on biota. Here, we present a metaanalysis of the effect of climate change on body size of ectothermic aquatic organisms (bacteria, phyto- and zooplankton, and fish) from the community to the individual level. Using long-term surveys, experimental data and published results, we show a significant increase in the proportion of small-sized species and young age classes and a decrease in size-at-age. These results are in accordance with the ecological rules dealing with the temperature–size relationships (i.e., Bergmann's rule, James' rule and Temperature–Size Rule). Our study provides evidence that reduced body size is the third universal ecological response to global warming in aquatic systems besides the shift of species ranges toward higher altitudes and latitudes and the seasonal shifts in life cycle events.
    Type: Article , PeerReviewed
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  • 14
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    National Academy of Sciences
    In:  PNAS Proceedings of the National Academy of Sciences of the United States of America, 102 (8). pp. 2826-2831.
    Publication Date: 2016-11-14
    Description: Contemporary climate change is characterized both by increasing mean temperature and increasing climate variability such as heat waves, storms, and floods. How populations and communities cope with such climatic extremes is a question central to contemporary ecology and biodiversity conservation. Previous work has shown that species diversity can affect ecosystem functioning and resilience. Here, we show that genotypic diversity can replace the role of species diversity in a species-poor coastal ecosystem, and it may buffer against extreme climatic events. In a manipulative field experiment, increasing the genotypic diversity of the cosmopolitan seagrass Zostera marina enhanced biomass production, plant density, and faunal abundance, despite near-lethal water temperatures due to extreme warming across Europe. Net biodiversity effects were explained by genotypic complementarity rather than by selection of particularly robust genotypes. Positive effects on invertebrate fauna suggest that genetic diversity has second-order effects reaching higher trophic levels. Our results highlight the importance of maintaining genetic as well as species diversity to enhance ecosystem resilience in a world of increasing uncertainty.
    Type: Article , PeerReviewed
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  • 15
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    National Academy of Sciences
    In:  PNAS Proceedings of the National Academy of Sciences of the United States of America, 104 (14). pp. 6049-6054.
    Publication Date: 2016-11-14
    Description: Lytic viral infection and programmed cell death (PCD) are thought to represent two distinct death mechanisms in phytoplankton, unicellular photoautotrophs that drift with ocean currents. Here, we demonstrate an interaction between autocatalytic PCD and lytic viral infection in the cosmopolitan coccolithophorid, Emiliania huxleyi. Successful infection of E. huxleyi strain 374 with a lytic virus, EhV1, resulted in rapid internal degradation of cellular components, a dramatic reduction in the photosynthetic efficiency (F-v/F-m), and an up-regulation of metacaspase protein expression, concomitant with induction of caspase-like activity. Caspase activation was confirmed through in vitro cleavage in cell extracts of the fluorogenic peptide substrate, IETD-AFC, and direct, in vivo staining of cells with the fluorescently labeled irreversible caspase inhibitor, FITC-VAD-FMK. Direct addition of z-VAD-FMK to infected cultures abolished cellular caspase activity and protein expression and severely impaired viral production. The absence of metacaspase protein expression in resistant E. huxleyi strain 373 during EhV1 infection further demonstrated the critical role of these proteases in facilitating viral lysis. Together with the presence of caspase cleavage recognition sequences within virally encoded proteins, we provide experimental evidence that coccolithoviruses induce and actively recruit host metacaspases as part of their replication strategy. These findings reveal a critical role for metacaspases in the turnover of phytoplankton biomass upon infection with viruses and point to coevolution of host-virus interactions in the activation and maintenance of these enzymes in planktonic, unicellular protists.
    Type: Article , PeerReviewed
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  • 16
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    National Academy of Sciences
    In:  PNAS Proceedings of the National Academy of Sciences of the United States of America, 106 . pp. 197-202.
    Publication Date: 2016-11-14
    Description: Fisheries can have a large impact on marine ecosystems, because the effects of removing large predatory fish may cascade down the food web. The implications of these cascading processes on system functioning and resilience remain a source of intense scientific debate. By using field data covering a 30-year period, we show for the Baltic Sea that the underlying mechanisms of trophic cascades produced a shift in ecosystem functioning after the collapse of the top predator cod. We identified an ecological threshold, corresponding to a planktivore abundance of ≈17 × 1010 individuals, that separates 2 ecosystem configurations in which zooplankton dynamics are driven by either hydroclimatic forces or predation pressure. Abundances of the planktivore sprat above the threshold decouple zooplankton dynamics from hydrological circumstances. The current strong regulation by sprat of the feeding resources for larval cod may hinder cod recovery and the return of the ecosystem to a prior state. This calls for the inclusion of a food web perspective in management decisions.
    Type: Article , PeerReviewed
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  • 17
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    National Academy of Sciences
    In:  PNAS Proceedings of the National Academy of Sciences of the United States of America, 105 (30). pp. 10438-10443.
    Publication Date: 2016-10-25
    Description: Marine primary productivity is iron (Fe)-limited in vast regions of the contemporary oceans, most notably the high nutrient low chlorophyll (HNLC) regions. Diatoms often form large blooms upon the relief of Fe limitation in HNLC regions despite their prebloom low cell density. Although Fe plays an important role in controlling diatom distribution, the mechanisms of Fe uptake and adaptation to low iron availability are largely unknown. Through a combination of nontargeted transcriptomic and metabolomic approaches, we have explored the biochemical strategies preferred by Phaeodactylum tricornutum at growth-limiting levels of dissolved Fe. Processes carried out by components rich in Fe, such as photosynthesis, mitochondrial electron transport, and nitrate assimilation, were down-regulated. Our results show that this retrenchment is compensated by nitrogen (N) and carbon (C) reallocation from protein and carbohydrate degradation, adaptations to chlorophyll biosynthesis and pigment metabolism, removal of excess electrons by mitochondrial alternative oxidase (AOX) and non-photochemical quenching (NPQ), and augmented Fe-independent oxidative stress responses. Iron limitation leads to the elevated expression of at least three gene clusters absent from the Thalassiosira pseudonana genome that encode for components of iron capture and uptake mechanisms.
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  • 18
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    National Academy of Sciences
    In:  PNAS Proceedings of the National Academy of Sciences of the United States of America, 106 (49). pp. 20578-20583.
    Publication Date: 2016-10-25
    Description: The El Niño/Southern Oscillation (ENSO) phenomenon, originating in the Tropical Pacific, is the strongest natural interannual climate signal and has widespread effects on the global climate system and the ecology of the Tropical Pacific. Any strong change in ENSO statistics will therefore have serious climatic and ecological consequences. Most global climate models do simulate ENSO, although large biases exist with respect to its characteristics. The ENSO response to global warming differs strongly from model to model and is thus highly uncertain. Some models simulate an increase in ENSO amplitude, others a decrease, and others virtually no change. Extremely strong changes constituting tipping point behavior are not simulated by any of the models. Nevertheless, some interesting changes in ENSO dynamics can be inferred from observations and model integrations. Although no tipping point behavior is envisaged in the physical climate system, smooth transitions in it may give rise to tipping point behavior in the biological, chemical, and even socioeconomic systems. For example, the simulated weakening of the Pacific zonal sea surface temperature gradient in the Hadley Centre model (with dynamic vegetation included) caused rapid Amazon forest die-back in the mid-twenty-first century, which in turn drove a nonlinear increase in atmospheric CO2, accelerating global warming.
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  • 19
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    National Academy of Sciences
    In:  PNAS Proceedings of the National Academy of Sciences of the United States of America, 104 . pp. 6556-6561.
    Publication Date: 2016-10-25
    Description: The Younger Dryas cold interval represents a time when much of the Northern Hemisphere cooled from �12.9 to 11.5 kiloyears B.P. The cause of this event, which has long been viewed as the canonical example of abrupt climate change, was initially attributed to the routing of freshwater to the St. Lawrence River with an attendant reduction in Atlantic meridional overturning circulation. However, this mechanism has recently been questioned because current proxies and dating techniques have been unable to confirm that eastward routing with an increase in freshwater flux occurred during the Younger Dryas. Here we use new geochemical proxies (�Mg/Ca, U/Ca, and 87Sr/86Sr) measured in planktonic foraminifera at the mouth of the St. Lawrence estuary as tracers of freshwater sources to further evaluate this question. Our proxies, combined with planktonic �18Oseawater and �13C, confirm that routing of runoff from western Canada to the St. Lawrence River occurred at the start of the Younger Dryas, with an attendant increase in freshwater flux of 0.06 +- 0.02 Sverdrup (1 Sverdrup = 106 m3s-1). This base discharge increase is sufficient to have reduced Atlantic meridional overturning circulation and caused the Younger Dryas cold interval. In addition, our data indicate subsequent fluctuations in the freshwater flux to the St. Lawrence River of ~0.06–0.12 Sverdrup, thus explaining the variability in the overturning circulation and climate during the Younger Dryas.
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  • 20
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    Washington, DC : National Academy of Sciences
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