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  • Articles  (16)
  • 2015-2019  (16)
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  • Articles  (16)
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  • 11
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    Plant Beneficial Endophytic Bacteria from the EthnomedicinalMussaenda roxburghii(Akshap) of Eastern Himalayan Province, India (2015)
    Hindawi
    Details
    Publication Date: 2015-01-01
    Description: Mussaenda roxburghiiare very important ethnomedicinal plant, used for its various applications from the ancient period. The role of their associated plant beneficial endophytic bacteria was evaluated, which were previously untapped. Among the isolates, PAK6 was identified as efficient phosphate solubilizer, quantified by the molybdenum blue method. Four isolates PAK1, PAK2, PAK3, and PAK8 were able to synthesize significant level of IAA in the presence and absence of tryptophan. Isolates PAK1 and PAK9 were able to produce siderophore on CAS agar media, PAK2 and PAK9 were able to produce HCN, and PAK7 and PAK8 were able to grow on N2-free medium. All the isolates were able to produce a moderate level of polysaccharide and tolerate up to 10% of NaCl. Isolates PAK3, PAK6, PAK7, and PAK8 were able to grow well at pH 5.0 and isolates PAK2, PAK7, and PAK8 were able to tolerate 600 μg mL−1of Al+3, while all the isolates except PAK1 showed a tolerance to 600 μg mL−1of Mn+2tested. Endophytic bacterial isolates PAK6 and PAK9 were effective againstSclerotinia sclerotiorumandSclerotium rolfsii.
    Print ISSN: 2356-6582
    Electronic ISSN: 2314-7563
    Topics: Biology
    Published by Hindawi
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  • 12
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    Sodium Butyrate Plus EGF and PDGF-BB Aids Cutaneous Wound Healing in Diabetic Mice (2015)
    Hindawi
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    Publication Date: 2015-01-01
    Description: Topical application of growth factors is known to aid defective and/or delayed wound healing in diabetic patients. In this study, the effect of topical application of sodium butyrate (Na-Bu), EGF, and PDGF-BB was analysed on the acute cutaneous wound healing in streptozotocin (STZ) induced diabetic mouse model. Two cutaneous wounds were created, one on each of the dorsolateral sides of the diabetic mice. Na-Bu, EGF, and PDGF-BB were applied to the wound either individually or in various combinations. The wound healing was monitored visually and scored as percentage wound closure. The tissue samples were collected from the wound site at 1, 7, and 14 days after wounding from the treated and untreated diabetic wounds and analysed for the levels of EGF-R,β-PDGF-R, HDAC1, p21, and phosphorylated and hypophosphorylated pRb proteins. Our results indicate that application of EGF plus PDGF-BB at the initial stages followed by subsequent addition of Na-Bu along with these growth factors helps wound healing in diabetic mice. It appears that, in addition to cell proliferative agents, a cell differentiation agent, Na-Bu, is necessary for diabetic wound healing. Topical application of EGF plus PDGF-BB along with Na-Bu could be developed as therapeutic agents to treat and manage human diabetic wounds.
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    Electronic ISSN: 2314-7563
    Topics: Biology
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  • 13
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    Histological and Biochemical Evaluation of the Kidney following Chronic Consumption of Hibiscus sabdariffa (2015)
    Hindawi
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    Publication Date: 2015-01-01
    Description: Hibiscus sabdariffa L. has been used traditionally as herbal medicine and has been documented to have a broad range of therapeutic effects. The present study aimed to investigate the effects of chronic administration of aqueous extract of flowers of Hibiscus sabdariffa on the histology of the kidney and some biochemical indices of renal function in male Wistar rats. Twenty (20) Wistar rats were randomly divided into four (4) groups of five rats each. The extract was administered orally in doses 200, 500, and 800 mg/kg body weight for 21 days. The kidney was harvested and processed histologically and blood samples were taken for biochemical assays. The histological results showed dose dependent pathological states and the biochemical analysis revealed a dose dependant variation in renal indices. These results suggest that chronic administration of aqueous extract of Hibiscus sabdariffa may be toxic to the kidney.
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    Topics: Biology
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  • 14
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    Direct Organogenesis from Rhizome Explants in Marsilea quadrifolia L.: A Threatened Fern Species (2015)
    Hindawi
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    Publication Date: 2015-01-01
    Description: An efficient micropropagation protocol has been developed for Marsilea quadrifolia L. through direct organogenesis. The mature rhizomes were used as explants and successfully sterilized using 0.1% HgCl2 for the establishment of cultures. The multiple shoots were differentiated from the explants on Murashige and Skoog (MS) medium augmented with 6-benzylaminopurin (BAP). Full strength MS medium was reported to be effective for the induction of sporophytes from the rhizomes after four weeks of inoculation. Maximum response (96%) with average of 6.2 shoots (2.72 cm length) was achieved on full strength of MS medium augmented with 0.5 mg/L BAP in culture initiation experiments. The cultures were further proliferated in clusters (79.0±0.37 shoots per explant) with stunted growth on half strength MS medium supplemented with 0.25 mg/L BAP after four weeks. These stunted shoots were elongated (5.30 cm long) on half MS medium devoid of growth hormones. Root induction and proliferation (3.0–4.0 cm long) were observed after 4th subculture of sporophytes on hormone-free half strength MS medium. The rooted plantlets were hardened in the fern house for 4-5 weeks and transferred to the field with 92% survival rate. There were no observable differences in between in vivo grown and in vitro propagated plantlets in the field.
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    Topics: Biology
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  • 15
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    Seasonal Changes in Ovarian Follicle Growth in Iran Viper (Vipera albicornuta) (2015)
    Hindawi
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    Publication Date: 2015-01-01
    Description: TheVipera albicornutais an economically important snake of Iran, its venom is used for antivenin production, and we need to breed the snake in the captivity. In order to know about the viper’s reproductive biology, seasonal alterations in ovarian weight, morphology, and the follicles developmental stages inVipera albicornutawere studied using macroscopic parameters and histological examination of reproductive tissues during a year. Twenty-four female vipers were collected from mountainous zone of Bostanabad, East Azerbaijan, and Tarom in Zanjan through years 2011-2012. Evaluation of the reproductive parameters of this viper is performed for the first time in Iran. Our observation revealed that vitellogenesis cycle begins in autumn and continues till early summer; ovaries and follicles are in previtellogenesis stage in autumn and vitellogenesis in winter and during spring. Weight of ovary is heaviest in spring, ovulation occurs in late spring till early summer, and copulation is prior to ovulation.
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    Topics: Biology
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  • 16
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    Role of Green Tea in Reducing Epidermal Thickness upon Ultraviolet Light-B Injury in BALB/c Mice (2015)
    Hindawi
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    Publication Date: 2015-01-01
    Description: The main environmental source for skin damage is ultraviolet (UV) radiation. Many adverse effects have been recognized as the result of prolonged cutaneous exposure to solar ultraviolet radiation, such as erythema, edema, apoptosis, hyperplastic responses, photo-aging, and skin cancer development. Green tea provides photo-protection against UV radiation through many mechanisms including anti-inflammatory, immunomodulatory, and antioxidant properties. The aim of this study was to evaluate the effect of green tea in reducing epidermal thickness on mouse’s skin exposed to UVB irradiation. Thirty mice (Mus musculus species, BALB/c strain) underwent this study and were divided into 3 groups: control group (n=10 mice), without UVB exposure and green tea administration; exposure group (n=10 mice), which were exposed to UVB light only; and treatment group (n=10 mice), which were exposed to UVB light and treated with 1 mL of green tea through oral gavage. Mice from both groups (exposure and treatment) were subjected to UVB irradiation 4 days/week (20 minutes/day, 4 weeks). It concluded that oral administration of green tea was provided photo-protection against UVB induced hyperplasia; therefore, it can be regarded as a natural alternative for photo-protection.
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    Topics: Biology
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