ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
Collection
Publisher
Years
  • 1
    Publication Date: 2012-10-13
    Description:    Activator/Dissociation ( Ac/Ds ) transposable elements have been used in maize insertional mutagenesis as a complement to Mutator ( Mu ). In this study, to further improve the efficiency of the Ac / Ds mutagenesis system, we adopted apt1 - m1 (Ac) on the long arm of chromosome 9 (9L) as a donor Ac to create an Ac insertion library. This system is based on the negative selection pressure against the donor Ac , and it was highly efficient for isolating new transposition events. We obtained 9,625 transposition events from 1083 F1 ears with an average transposition rate of 8.66 % (rates ranged from 1.11 to 29.73 %). We also adopted a modified PCR-based genome walking strategy to improve the efficiency of the new method for isolating transposon-flanking sequences. This method is more efficient than the Southern-based method that was used in previous studies. A validation step was developed to distinguish transposon tags derived from newly transposed Ac or Ds elements. Using this PCR-based method, we isolated 67 inheritable flanking sequences from the apt1 - m1 (Ac) transposition library; of these, 51 were confirmed as tr- Ac -flanking sequences and 11 were tr- Ds -flanking sequences. Similar to other Ac donors from different loci, the apt1 - m1 (Ac) system also exhibited a preference for short distance transposition. In this study, we have further improved the Ac mutagenesis system in maize for gene isolation and functional genomics studies. Content Type Journal Article Pages 1-11 DOI 10.1007/s10709-012-9685-2 Authors Fei Wang, School of Life Sciences, Shanghai Key Laboratory of Bio-energy Crop, Shanghai University, 333 Nanchen Road, Shanghai, 200444 People’s Republic of China Pengfei Li, School of Life Sciences, Shanghai Key Laboratory of Bio-energy Crop, Shanghai University, 333 Nanchen Road, Shanghai, 200444 People’s Republic of China Yuanping Tang, School of Life Sciences, Shanghai Key Laboratory of Bio-energy Crop, Shanghai University, 333 Nanchen Road, Shanghai, 200444 People’s Republic of China Jun Fan, School of Life Sciences, Shanghai Key Laboratory of Bio-energy Crop, Shanghai University, 333 Nanchen Road, Shanghai, 200444 People’s Republic of China Dabin Xu, School of Life Sciences, Shanghai Key Laboratory of Bio-energy Crop, Shanghai University, 333 Nanchen Road, Shanghai, 200444 People’s Republic of China Shengming Guo, School of Life Sciences, Shanghai Key Laboratory of Bio-energy Crop, Shanghai University, 333 Nanchen Road, Shanghai, 200444 People’s Republic of China Zhengkai Xu, School of Life Sciences, Shanghai Key Laboratory of Bio-energy Crop, Shanghai University, 333 Nanchen Road, Shanghai, 200444 People’s Republic of China Rentao Song, School of Life Sciences, Shanghai Key Laboratory of Bio-energy Crop, Shanghai University, 333 Nanchen Road, Shanghai, 200444 People’s Republic of China Journal Genetica Online ISSN 1573-6857 Print ISSN 0016-6707
    Print ISSN: 0016-6707
    Electronic ISSN: 1573-6857
    Topics: Biology
    Published by Springer
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    Publication Date: 2012-10-20
    Description:    We analyze here the presence and abundance of three types of transposable elements (TEs), i.e. Gypsy , RTE and Mariner , in the genome of the grasshopper Eyprepocnemis plorans . PCR experiments allowed amplification, cloning and sequencing of these elements ( EploGypI , EploRTE5 , EploMar20 ) from the E. plorans genome. Fluorescent in situ hybridization (FISH) showed that all three elements are restricted to euchromatic regions, thus being absent from the pericentromeric region of all A chromosomes, which contain a satellite DNA (satDNA) and ribosomal DNA (rDNA), and being very scarce in B chromosomes mostly made up of these two types of repetitive DNA. FISH suggested that EploGypI is the most abundant and EploMar20 is the least abundant, with EploRTE5 showing intermediate abundance. An estimation of copy number, by means of quantitative PCR, showed that EploGypI is, by far, the most abundant element, followed by EploRTE5 and EploMar20 , in consistency with FISH results. RNA isolation and PCR experiments on complementary DNA (cDNA) showed the presence of transcripts for the three TE elements. The implications of the preferential location of these TE elements into euchromatin, the significance of TE abundance in the giant genome of this species, and a possible relationship between TEs and B chromosome mutability, are discussed. Content Type Journal Article Pages 1-10 DOI 10.1007/s10709-012-9686-1 Authors Eugenia E. Montiel, Departamento de Genética, Universidad de Granada, 18071 Granada, Spain Josefa Cabrero, Departamento de Genética, Universidad de Granada, 18071 Granada, Spain Juan Pedro M. Camacho, Departamento de Genética, Universidad de Granada, 18071 Granada, Spain Mª Dolores López-León, Departamento de Genética, Universidad de Granada, 18071 Granada, Spain Journal Genetica Online ISSN 1573-6857 Print ISSN 0016-6707
    Print ISSN: 0016-6707
    Electronic ISSN: 1573-6857
    Topics: Biology
    Published by Springer
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 3
    Publication Date: 2012-10-20
    Description:    The major histocompatibility complex (MHC) is central to the vertebrate immune system and its highly polymorphic genes are considered to influence several life-history traits of individuals. To characterize the MHC in a natural population of blue tits ( Cyanistes caeruleus ) we investigated the class I exon 3 diversity of more than 900 individuals. We designed two pairs of motif-specific primers that reliably amplify independent subsets of MHC alleles. Applying denaturing gradient gel electrophoresis (DGGE) we obtained 48 independently inherited units of unique band patterns (DGGE-haplogroups), which were validated in a segregation analysis within 105 families. In a second approach, we extensively sequenced 6 unrelated individuals to confirm that DGGE-haplogroup composition reflects individual allelic variation. The highest number of different DGGE-haplogroups in a single individual corresponded in 19 MHC exon 3 sequences, suggesting a minimum of 10 amplified MHC class I loci in the blue tit. In total, we identified 50 unique functional and 3 non-functional sequences. Functional sequences showed high levels of recombination and strong positive selection in the antigen binding region, whereas nucleotide diversity was comparatively low in the range of all passerine species. Finally, in a phylogenetic comparison of passerine MHC class I exon 3 sequences we discuss conflicting evolutionary signals possibly due to recent gene duplication, recombination events and concerted evolution. Our results indicate that the described method is suitable to effectively explore the MHC diversity and its ecological impacts in blue tits in future studies. Content Type Journal Article Pages 1-16 DOI 10.1007/s10709-012-9679-0 Authors R. Wutzler, Department of Behavioural Ecology and Evolutionary Genetics, Max Planck Institute for Ornithology, 82305 Seewiesen, Germany K. Foerster, Comparative Zoology, Institute of Evolution and Ecology, University of Tübingen, D-72076 Tübingen, Germany B. Kempenaers, Department of Behavioural Ecology and Evolutionary Genetics, Max Planck Institute for Ornithology, 82305 Seewiesen, Germany Journal Genetica Online ISSN 1573-6857 Print ISSN 0016-6707
    Print ISSN: 0016-6707
    Electronic ISSN: 1573-6857
    Topics: Biology
    Published by Springer
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 4
    Publication Date: 2012-04-17
    Description:    The wolf fish Hoplias malabaricus includes well differentiated sex systems (XY and X 1 X 2 Y in karyomorphs B and D, respectively), a nascent XY pair (karyomorph C) and not recognized sex chromosomes (karyomorph A). We performed the evolutionary analysis of these sex chromosomes, using two X chromosome-specific probes derived by microdissection from the XY and X 1 X 2 Y sex systems. A putative-sex pair in karyomorph A was identified, from which the differentiated XY system was evolved, as well as the clearly evolutionary relationship between the nascent XY system and the origin of the multiple X 1 X 2 Y chromosomes. The lack of recognizable signals on the sex chromosomes after the reciprocal cross-FISH experiments highlighted that they evolved independently from non-homologous autosomal pairs. It is noteworthy that these distinct pathways occur inside the same nominal species, thus exposing the high plasticity of sex chromosome evolution in lower vertebrates. Possible mechanisms underlying this sex determination liability are also discussed. Content Type Journal Article Pages 1065-1072 DOI 10.1007/s10709-011-9610-0 Authors Marcelo de Bello Cioffi, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil Antonio Sánchez, Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain Juan Alberto Marchal, Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain Nadezda Kosyakova, Institute of Human Genetics, Jena University Hospital, Kollegiengasse 10, 07743 Jena, Germany Thomas Liehr, Institute of Human Genetics, Jena University Hospital, Kollegiengasse 10, 07743 Jena, Germany Vladimir Trifonov, Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia Luiz Antonio Carlos Bertollo, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil Journal Genetica Online ISSN 1573-6857 Print ISSN 0016-6707 Journal Volume Volume 139 Journal Issue Volume 139, Number 8
    Print ISSN: 0016-6707
    Electronic ISSN: 1573-6857
    Topics: Biology
    Published by Springer
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 5
    Publication Date: 2012-04-17
    Description: Erratum to: Salamandra salamandra (Amphibia: Caudata: Salamandridae) in Portugal: not all black and yellow Content Type Journal Article Category Erratum Pages 1107-1107 DOI 10.1007/s10709-011-9612-y Authors Davide M. Reis, Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal Regina L. Cunha, Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal Cláudia Patrão, Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal Rui Rebelo, Centro de Biologia Ambiental e Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa, Bloco C2, Campo Grande, 1749-016 Lisbon, Portugal Rita Castilho, Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal Journal Genetica Online ISSN 1573-6857 Print ISSN 0016-6707 Journal Volume Volume 139 Journal Issue Volume 139, Number 9
    Print ISSN: 0016-6707
    Electronic ISSN: 1573-6857
    Topics: Biology
    Published by Springer
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 6
    Publication Date: 2012-04-17
    Description:    Salinomys delicatus is considered a rare species due to its restricted and patchy distribution, poor records and low abundances. It is also the phyllotine with the lowest known diploid chromosome number (2n = 18), however its sex chromosome system has never been described. Here, we studied the chromosomes of six females and three males with bands G, C, DAPI/CMA 3 and meiosis. In males, the chromosome number was 2n = 19, with one large metacentric X-chromosome and two medium-sized acrocentrics absent in females. The karyotype of females was the same as previously described (2n = 18, FN = 32), with X-chromosomes being metacentric and the largest elements of the complement. In males, the two acrocentrics and the large metacentric form a trivalent in meiotic prophase. This indicates that S. delicatus has XY 1 Y 2 sex chromosomes, which is confirmed by G and DAPI bands. Constitutive heterochromatin (CH) is restricted to small pericentromeric blocks in all chromosomes. The X-chromosome shows the largest block of centromeric CH, which could favor the establishment of this X-autosome translocation. This sex chromosome system is rare in mammals and, compared with other phyllotine rodents, S. delicatus seems to have undergone a major chromosome restructuring during its karyotypic evolution. Content Type Journal Article Pages 1143-1147 DOI 10.1007/s10709-011-9616-7 Authors C. Lanzone, Grupo de Investigaciones de la Biodiversidad, IADIZA, CONICET, CCT-Mendoza, CC 507, CP5500 Mendoza, Argentina D. Rodríguez, Grupo de Investigaciones de la Biodiversidad, IADIZA, CONICET, CCT-Mendoza, CC 507, CP5500 Mendoza, Argentina P. Cuello, Grupo de Investigaciones de la Biodiversidad, IADIZA, CONICET, CCT-Mendoza, CC 507, CP5500 Mendoza, Argentina S. Albanese, Grupo de Investigaciones de la Biodiversidad, IADIZA, CONICET, CCT-Mendoza, CC 507, CP5500 Mendoza, Argentina A. Ojeda, Grupo de Investigaciones de la Biodiversidad, IADIZA, CONICET, CCT-Mendoza, CC 507, CP5500 Mendoza, Argentina V. Chillo, Grupo de Investigaciones de la Biodiversidad, IADIZA, CONICET, CCT-Mendoza, CC 507, CP5500 Mendoza, Argentina D. A. Martí, Laboratorio de Genética Evolutiva, Universidad Nacional de Misiones, CONICET, Félix de Azara 1552, CP3300 Posadas, Misiones, Argentina Journal Genetica Online ISSN 1573-6857 Print ISSN 0016-6707 Journal Volume Volume 139 Journal Issue Volume 139, Number 9
    Print ISSN: 0016-6707
    Electronic ISSN: 1573-6857
    Topics: Biology
    Published by Springer
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 7
    Publication Date: 2012-04-17
    Description:    The relative role of sexual reproduction and mutation in shaping the diversity of clonally propagated crops is largely unknown. We analyzed the genetic diversity of yam—a vegetatively-propagated crop—to gain insight into how these two factors shape its diversity in relation with farmers’ classifications. Using 15 microsatellite loci, we analyzed 485 samples of 10 different yam varieties. We identified 33 different genotypes organized in lineages supported by high bootstrap values. We computed the probability that these genotypes appeared by sexual reproduction or mutation within and between each lineage. This allowed us to interpret each lineage as a product of sexual reproduction that has evolved by mutation. Moreover, we clearly noted a similarity between the genetic structure and farmers’ classifications. Each variety could thus be interpreted as being the product of sexual reproduction having evolved by mutation. This highly structured diversity of farmer-managed varieties has consequences for the preservation of yam diversity. Content Type Journal Article Pages 1055-1064 DOI 10.1007/s10709-011-9607-8 Authors N. Scarcelli, Institut de Recherche pour le Développement (IRD), Equipe DYNADIV, UMR Diversité Adaptation Développement des Plantes (DIADE), 911 Avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France S. Tostain, Institut de Recherche pour le Développement (IRD), Equipe DYNADIV, UMR Diversité Adaptation Développement des Plantes (DIADE), 911 Avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France Y. Vigouroux, Institut de Recherche pour le Développement (IRD), Equipe DYNADIV, UMR Diversité Adaptation Développement des Plantes (DIADE), 911 Avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France V. Luong, Institut de Recherche pour le Développement (IRD), Equipe DYNADIV, UMR Diversité Adaptation Développement des Plantes (DIADE), 911 Avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France M. N. Baco, Université de Parakou, BP 123 Parakou, Benin C. Agbangla, Laboratoire de Génétique, Université d’Abomey-Calavi, FAST, BP 526 Cotonou, Benin O. Daïnou, Laboratoire de Génétique, Université d’Abomey-Calavi, FAST, BP 526 Cotonou, Benin J. L. Pham, Institut de Recherche pour le Développement (IRD), Equipe DYNADIV, UMR Diversité Adaptation Développement des Plantes (DIADE), 911 Avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France Journal Genetica Online ISSN 1573-6857 Print ISSN 0016-6707 Journal Volume Volume 139 Journal Issue Volume 139, Number 8
    Print ISSN: 0016-6707
    Electronic ISSN: 1573-6857
    Topics: Biology
    Published by Springer
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 8
    facet.materialart.
    Unknown
    Springer
    In: Genetica
    Publication Date: 2012-04-17
    Description:    The notion of intragenomic bet-hedging is introduced by modeling a system where one locus is seen as setting the “environment” for selection in a two-locus genetic system. Using a spatially structured simulation model I show that bet-hedging alleles with a lower mean fitness and lower variance of fitness across genotypes at a different locus can go to fixation, potentially providing a mechanism for the reduction of severe heterozygote advantage. Content Type Journal Article Pages 1019-1021 DOI 10.1007/s10709-011-9603-z Authors Jostein Starrfelt, Norwegian Institute for Water Research, Gaustadalléen 21, 0349 Oslo, Norway Journal Genetica Online ISSN 1573-6857 Print ISSN 0016-6707 Journal Volume Volume 139 Journal Issue Volume 139, Number 8
    Print ISSN: 0016-6707
    Electronic ISSN: 1573-6857
    Topics: Biology
    Published by Springer
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 9
    Publication Date: 2012-04-17
    Description:    The leucine-rich repeat (LRR) receptor kinase (RLK) proteins constitute a large superfamily in the plant genome, and carry out key functions in a variety of biological pathways. In an effort to determine the evolutionary fate of members of a large gene family such as plant LRR RLK proteins we conducted in silico analysis using complete genome sequencing datasets, genome-wide transcriptome databases, and bioinformatics tools. A total of 292 and 165 LRR RLK genes were retrieved from the rice and Arabidopsis genomes, respectively, formed by diverse duplication events for gene expansion. The phylogenic analyses of the LRR RLK genes suggested combinations of LRR domains and RLK domains in the ancient plant genome prior to the divergence of rice and Arabidopsis , followed by massive independent expansions during speciation. The somewhat high frequencies (50–73%) of expressional divergence of members of duplicate gene pairs formed by whole/segmental genome duplication (W/SGD) and tandem duplication (TD) events of Arabidopsis and TD events of rice support the idea of their functional diversity for gene retention. By contrast, a relatively low degree (at least 20%) of members of rice LRR RLK gene pairs formed by W/SGD appear to be divergent in expression following the duplication event. At least 7 pairs of co-expressed gene clusters, including each of the tentative orthologous LRR RLK genes between rice and Arabidopsis, were enriched to an orthologous set between members of each of the pairs as compared to those of the random pairs, suggesting some degree of functional conservation of individual genes. These results may shed some light on the crucial functions of the plant LRR RLK genes with regard to a variety of biological processes. Content Type Journal Article Pages 1023-1032 DOI 10.1007/s10709-011-9604-y Authors Sun-Goo Hwang, Plant Genomics Lab, Department of Applied Plant Sciences, Kangwon Natl University, Chuncheon, 200-713 Korea Dong Sub Kim, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 580-185 Korea Cheol Seong Jang, Plant Genomics Lab, Department of Applied Plant Sciences, Kangwon Natl University, Chuncheon, 200-713 Korea Journal Genetica Online ISSN 1573-6857 Print ISSN 0016-6707 Journal Volume Volume 139 Journal Issue Volume 139, Number 8
    Print ISSN: 0016-6707
    Electronic ISSN: 1573-6857
    Topics: Biology
    Published by Springer
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 10
    Publication Date: 2012-04-17
    Description:    The fire salamander complex is quite diverse in the Iberian Peninsula where nine subspecies of Salamandra salamandra are currently recognized. Here, we analysed the geographical distribution of the subspecies S. s. gallaica and S. s. crespoi using partial sequences of the mitochondrial cytochrome b gene of 168 individuals from 12 locations in Portugal. Our results support the existence of a deep lineage divergence between the two subspecies, with non-overlapping geographical distributions except in two contact zones: one in Sesimbra on the western coast, and another in Alcoutim on the southeastern border with Spain. Moreover, S. s. crespoi displays signs of gene flow among the sampled locations whereas S. s. gallaica shows evidence of some restriction to gene flow. Present-day genetic make-up of S. s. gallaica and S. s. crespoi is a result of past historical events, fine-tuned by contemporary Iberian geoclimate. Humid mountain areas were found to harbour increased genetic diversity possibly acting as past refugia during drier interglacial periods. To analyse wider geographical patterns and lineage splitting events within S. salamandra we performed a Bayesian dating analysis completing our data set with previously published sequences. The observed divergences were associated to successive biogeographic scenarios, and to other Iberian species showing similar trends. Content Type Journal Article Pages 1095-1105 DOI 10.1007/s10709-011-9609-6 Authors Davide M. Reis, Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal Regina L. Cunha, Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal Cláudia Patrão, Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal Rui Rebelo, Centro de Biologia Ambiental e Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa, Bloco C2, Campo Grande, 1749-016 Lisbon, Portugal Rita Castilho, Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal Journal Genetica Online ISSN 1573-6857 Print ISSN 0016-6707 Journal Volume Volume 139 Journal Issue Volume 139, Number 9
    Print ISSN: 0016-6707
    Electronic ISSN: 1573-6857
    Topics: Biology
    Published by Springer
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...