Publication Date:
2024-05-05
Description:
During expedition EMB201 in the Baltic Sea we investigated the local producers of glycerol dialkyl glycerol tetraethers (GDGT) and their thriving depth by a combined 16S rRNA gene amplicon sequencing/ CARD-FISH and lipidomic approach. Water samples were taken in December 2018 by a pump-CTD, a giant water sampler and with Niskin bottles at the surface, suboxic and sulfidic zones of the Landsort Deep, Fårö Basin and East Gotland Basin. This data set contains the CARD-FISH and lipidomics data, while the 16S rRNA gene sequencing data is available on ENA. Cell abundance was analysed in an aliquot of 40 ml filtered (pore size 0.22 micro m) sea water fixed with particle-free formaldehyde. Archaeal cells on the filters were specifically hybridized via catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) using the Cren537 probe. Cells on the hybridized filter were counter-stained with 40,6-diamidin-2-phenylindol (DAPI). For lipid analysis, 150–600 L sea water were filtered with a flow rate of 1.5 L min-1 on pre-ashed, 142-mm-diameter, 0.7µm pore size glass fibre GF/F filters, and frozen at –20 °C. The filters were lyophilized before different extraction methods were used to obtain intact and core GDGTs by ultra-sonification in different solvent mixtures. The combined supernatants were phase separated before analysis by high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS2) for intact polar lipids and by high performance liquid chromatography coupled to atmospheric pressure chemical ionization mass spectrometry (HPLC APCI-MS; ThermoScientific) for core GDGTs.
Keywords:
Archaea; Baltic Sea; BaltRap; BUCKET; Bucket water sampling; CARD-FISH; Catalyzed reporter deposition fluorescence in situ hybridisation (CARD-FISH); Cells, 4',6-Diamidin-2-phenylindol stained; Core acyclic glycerol dialkyl glycerol tetraether; Core crenarchaeol; Core crenarchaeol regio-isomer; Core dicyclic glycerol dialkyl glycerol tetraether; core lipids; Core monocyclic glycerol dialkyl glycerol tetraether; Core tricyclic glycerol dialkyl glycerol tetraether; Crenarchaeota, targeted with Cren537 oligonucleotide FISH-probe; CTD, Sea-Bird, SBE 911plus; CTD/Rosette; CTD-RO; DATE/TIME; DEPTH, water; Dihexose acyclic glycerol dialkyl glycerol tetraether; Dihexose-archaeol; Dihexose crenarchaeol; Dihexose dicyclic glycerol dialkyl glycerol tetraether; Dihexose monocyclic glycerol dialkyl glycerol tetraether; Dihexose tricyclic glycerol dialkyl glycerol tetraether; Eastern Gotland Basin; Elisabeth Mann Borgese; EMB201; EMB201_10-3; EMB201_10-9; EMB201_12-0; EMB201_12-4; EMB201_12-5; EMB201_5-3; EMB201_7-0; EMB201_7-1; EMB201_9-0; EMB201_9-1; Event label; Fårö Basin; glycerol dialkyl glycerol tetraethers; GOFLO; Go-Flo bottles; GPUMP; High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC; Intact polar lipids; Landsort Deep; LATITUDE; LC-MS/MS; Location; LONGITUDE; Monohexose acyclic glycerol dialkyl glycerol tetraether; Monohexose-archaeol; Monohexose crenarchaeol; Monohexose crenarchaeol regio-isomer; Monohexose dicyclic glycerol dialkyl glycerol tetraether; Monohexose-macroarchaeol; Monohexose monocyclic glycerol dialkyl glycerol tetraether; Monohexose tricyclic glycerol dialkyl glycerol tetraether; Other event; Oxygen, dissolved; Phosphohexose acyclic glycerol dialkyl glycerol tetraether; Phosphohexose crenarchaeol; Phosphohexose dicyclic glycerol dialkyl glycerol tetraether; Phosphohexose monocyclic glycerol dialkyl glycerol tetraether; Phosphohexose tricyclic glycerol dialkyl glycerol tetraether; Sample code/label; Ships non-toxic pump; Site; Stat. 06; Stat. TF 271 Stat. 4; Station 04 (TF 271); Station 09; Station label; Temperature, water; Ultra high performance liquid chromatography (UHPLC), Dionex Corporation, UltiMate 3000 RS; coupled with Single quadrupole mass spectrometer, Thermo Scientific, MSQ Plus
Type:
Dataset
Format:
text/tab-separated-values, 371 data points
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